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Protein

Voltage-dependent calcium channel subunit alpha-2/delta-3

Gene

Cacna2d3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) but not T-type (CACNA1G).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi262Divalent metal cationBy similarity1
Metal bindingi264Divalent metal cationBy similarity1
Metal bindingi266Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5576892. Phase 0 - rapid depolarisation.
R-MMU-5576893. Phase 2 - plateau phase.
R-MMU-5576894. Phase 1 - inactivation of fast Na+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent calcium channel subunit alpha-2/delta-3
Alternative name(s):
Voltage-gated calcium channel subunit alpha-2/delta-3
Cleaved into the following 2 chains:
Gene namesi
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1338890. Cacna2d3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini34 – 1007ExtracellularSequence analysisAdd BLAST974
Transmembranei1069 – 1089HelicalSequence analysisAdd BLAST21
Topological domaini1090 – 1091CytoplasmicSequence analysis2

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000304651? – 1091Voltage-dependent calcium channel subunit delta-3Sequence analysis
Signal peptidei1 – 33Sequence analysisAdd BLAST33
ChainiPRO_000030464934 – 1091Voltage-dependent calcium channel subunit alpha-2/delta-3Add BLAST1058
ChainiPRO_000030465034 – ?Voltage-dependent calcium channel subunit alpha-2-3Sequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Glycosylationi309N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi412 ↔ 1055Interchain (between alpha-2-3 and delta-3 chains)By similarity
Glycosylationi553N-linked (GlcNAc...)Sequence analysis1
Glycosylationi632N-linked (GlcNAc...)Sequence analysis1
Modified residuei924PhosphotyrosineBy similarity1

Post-translational modificationi

N-glycosylated.1 Publication
May be proteolytically processed into subunits alpha-2-3 and delta-3 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9Z1L5.
PaxDbiQ9Z1L5.
PeptideAtlasiQ9Z1L5.
PRIDEiQ9Z1L5.

PTM databases

PhosphoSitePlusiQ9Z1L5.

Expressioni

Tissue specificityi

Brain-specific. Predominantly expressed in the caudate putamen, entorhinal complex, hippocampus and cortex.2 Publications

Gene expression databases

BgeeiENSMUSG00000021991.
GenevisibleiQ9Z1L5. MM.

Interactioni

Subunit structurei

Dimer formed of alpha-2-2 and delta-2 chains; disulfide-linked. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1 (CACNA1), alpha-2 (CACNA2D), beta (CACNB) and delta (CACNA2D) subunits in a 1:1:1:1 ratio (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9Z1L5. 1 interactor.
STRINGi10090.ENSMUSP00000022567.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1L5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini256 – 438VWFAPROSITE-ProRule annotationAdd BLAST183
Domaini452 – 549CacheAdd BLAST98

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi262 – 266MIDAS-like motif5

Domaini

The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.By similarity

Sequence similaritiesi

Contains 1 cache domain.Curated
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2353. Eukaryota.
ENOG410XPDX. LUCA.
GeneTreeiENSGT00530000062904.
HOGENOMiHOG000010247.
HOVERGENiHBG107124.
InParanoidiQ9Z1L5.
KOiK04860.
OMAiAALDGKC.
OrthoDBiEOG091G00UY.
PhylomeDBiQ9Z1L5.
TreeFamiTF315824.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamiPF08473. VGCC_alpha2. 1 hit.
PF13768. VWA_3. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1L5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPGSLCCA SRGASALLAT ALLYAALGDV VRSEQQIPLS VVKLWASAFG
60 70 80 90 100
GEIKSIAAKY SGSQLLQKKY KEYEKDVAIE EIDGLQLVKK LAKIMEEMFH
110 120 130 140 150
KKSEAVRRLV EAAEEAHLKH EFDADLQYEY FNAVLINERD KDGNFLELGK
160 170 180 190 200
EFILAPNDHF NNLPVNISLS DVQVPTNMYN KDPAIVNGVY WSESLNKVFV
210 220 230 240 250
DNFDRDPSLI WQYFGSAKGF FRQYPGIKWE PDENGVIAFD CRNRKWYIQA
260 270 280 290 300
ATSPKDVVIL VDVSGSMKGL RLTIAKQTVS SILDTLGDDD FFNIITYNEE
310 320 330 340 350
LHYVEPCLNG TLVQADRTNK EHFREHLDKL FAKGIGMLDI ALNEAFNILS
360 370 380 390 400
DFNHTGQGSI CSQAIMLITD GAVDTYDTIF AKYNWPDRKV RIFTYLIGRE
410 420 430 440 450
AAFADNLKWM ACANKGFFTQ ISTLADVQEN VMEYLHVLSR PKVIDQEHDV
460 470 480 490 500
VWTEAYIDST LPQAQKLADD QGLVLMTTVA MPVFSKQNET RSKGILLGVV
510 520 530 540 550
GTDVPVKELL KTIPKYKLGI HGYAFAITNN GYILTHPELR PLYEEGKKRR
560 570 580 590 600
KPNYSSVDLS EVEWEDRDDV LRNAMVNRKT GKFSMEVKKT VDKGKRVLVM
610 620 630 640 650
TNDYYYTDIK GTPFSLGVAL SRGHGKYFFR GNVTIEEGLH DLEHPDVSLA
660 670 680 690 700
DEWSYCNTDL HPEHRHLSQL EAIKLYLKGK EPLLQCDKEL IQEVLFDAVV
710 720 730 740 750
SAPIEAYWTS LALNKSENSD KGVEVAFLGT RTGLSRINLF VGAEQLTNQD
760 770 780 790 800
FLKAGDKENI FNADHFPLWY RRAAEQIAGS FVYSIPFSTG TVNKSNVVTA
810 820 830 840 850
STSIQLLDER KSPVVAAVGI QMKLEFFQRK FWTASRQCAS LDGKCSISCD
860 870 880 890 900
DETVNCYLID NNGFILVSED YTQTGDFFGE VEGAVMNKLL TMGSFKRITL
910 920 930 940 950
YDYQAMCRAN KESSDSAHGL LDPYKAFLSA AKWIMTELVL FLVEFNLCSW
960 970 980 990 1000
WHSDMTAKAQ KLKQTLEPCD TEYPAFVSER TIKETTGNIA CEDCSKSFVI
1010 1020 1030 1040 1050
QQIPSSNLFM VVVDSSCLCE SVAPITMAPI EIRYNESLKC ERLKAQKIRR
1060 1070 1080 1090
RPESCHGFHP EENARECGGA SSLQAQAALL LLPLVSSLFS R
Length:1,091
Mass (Da):122,778
Last modified:May 1, 1999 - v1
Checksum:i7AEE2BDA10077A0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010949 mRNA. Translation: CAA09423.1.
PIRiT30256.
RefSeqiNP_033915.1. NM_009785.1.
UniGeneiMm.386754.

Genome annotation databases

EnsembliENSMUST00000022567; ENSMUSP00000022567; ENSMUSG00000021991.
GeneIDi12294.
KEGGimmu:12294.
UCSCiuc007sui.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010949 mRNA. Translation: CAA09423.1.
PIRiT30256.
RefSeqiNP_033915.1. NM_009785.1.
UniGeneiMm.386754.

3D structure databases

ProteinModelPortaliQ9Z1L5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z1L5. 1 interactor.
STRINGi10090.ENSMUSP00000022567.

PTM databases

PhosphoSitePlusiQ9Z1L5.

Proteomic databases

MaxQBiQ9Z1L5.
PaxDbiQ9Z1L5.
PeptideAtlasiQ9Z1L5.
PRIDEiQ9Z1L5.

Protocols and materials databases

DNASUi12294.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022567; ENSMUSP00000022567; ENSMUSG00000021991.
GeneIDi12294.
KEGGimmu:12294.
UCSCiuc007sui.1. mouse.

Organism-specific databases

CTDi55799.
MGIiMGI:1338890. Cacna2d3.

Phylogenomic databases

eggNOGiKOG2353. Eukaryota.
ENOG410XPDX. LUCA.
GeneTreeiENSGT00530000062904.
HOGENOMiHOG000010247.
HOVERGENiHBG107124.
InParanoidiQ9Z1L5.
KOiK04860.
OMAiAALDGKC.
OrthoDBiEOG091G00UY.
PhylomeDBiQ9Z1L5.
TreeFamiTF315824.

Enzyme and pathway databases

ReactomeiR-MMU-5576892. Phase 0 - rapid depolarisation.
R-MMU-5576893. Phase 2 - plateau phase.
R-MMU-5576894. Phase 1 - inactivation of fast Na+ channels.

Miscellaneous databases

ChiTaRSiCacna2d3. mouse.
PROiQ9Z1L5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021991.
GenevisibleiQ9Z1L5. MM.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR013680. VDCC_a2/dsu.
IPR013608. VWA_N.
IPR002035. VWF_A.
[Graphical view]
PfamiPF08473. VGCC_alpha2. 1 hit.
PF13768. VWA_3. 1 hit.
PF08399. VWA_N. 1 hit.
[Graphical view]
SMARTiSM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
PROSITEiPS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCA2D3_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1L5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to CACNA2D1 and CACNA2D2, it does not bind gabapentin, an antiepileptic drug.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.