Reviewed, UniProtKB/Swiss-Prot Q9Z1L0 (PK3CB_RAT)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform
      Short name=PtdIns-3-kinase subunit beta
      Short name=PI3-kinase subunit beta
      Short name=PI3K-beta
    EC=2.7.1.153
Alternative name(s):
    Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta
      Short name=PtdIns-3-kinase subunit p110-beta

Gene names

Name:

Pik3cb

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1070 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2 By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate.
Pathway	Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Subunit structure	Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphoinositide phosphorylation Inferred from electronic annotation. Source: InterPro phosphoinositide-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular component	brush border membrane Inferred from direct assay. Source: RGD phosphoinositide 3-kinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	1-phosphatidylinositol-3-kinase activity Inferred from direct assay. Source: RGD ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol-4,5-bisphosphate 3-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1070	1070	Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform		PRO_0000088789
Regions
	Domain	800 – 1050	251	PI3K/PI4K

Sequences

	Sequence		Length	Mass (Da)
	Q9Z1L0-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 4E8EB2333E96E4D5	FASTA	1,070	122,608
	10 20 30 40 50 60 MCFRSIMPPA MADTLDIWAV DSQIASDGSI SVDFLLPTGI YIQLEVPREA TISYIKQMLW 70 80 90 100 110 120 KQVHNYPMFN LLMDIDSYMF ACVNQTAVYE ELEDETRRLC DVRPFLPVLK LVTRSCDPAE 130 140 150 160 170 180 KLDSKIGVLI GKGLHEFDAL KDPEVNEFRR KMRKFSEDKI QSLVGLSWID WLKHTYPPEH 190 200 210 220 230 240 EPSVLENLED KLYGGKLVVA VHFENSQDVF SFQVSPNLNP IKINELAIQK RLTIRGKEEE 250 260 270 280 290 300 ASPCDYVLQV SGRVEYVFGD HPLIQFQYIR NCVMNRTLPH FILVECCKIK KMYEQEMIAI 310 320 330 340 350 360 EAAINRNSSS LPLPLPPKKT RVISHVWGNN NPFQIVLVKG NKLNTEETVK VHVRAGLFHG 370 380 390 400 410 420 TELLCKTVVS SEISGKNDHI WNEQLEFDIN ICDLPRMARL CFAVYAVLDK VKTKKSTKTI 430 440 450 460 470 480 NPSKYQTIRK AGKVHYPVAW VNTMVFDFKG QLRSGDVILH SWSSFPDELE EMLNPMGTVQ 490 500 510 520 530 540 TNPYAENATA LHIKFPENKK QPYYYPPFDK IIEKAAEIAS GDSANVSSRG GKKFLAVLKE 550 560 570 580 590 600 ILDRDPLSQL CENEMDLIWT LRQDCRENFP QSLPKLLLSI KWNKLEDVAQ LQALLQIWPK 610 620 630 640 650 660 LPPREALELL DFNYPDQYVR EYAVGCLRQM SDEELSQYLL QLVQVLKYEP FLDCALSRFL 670 680 690 700 710 720 LERALDNRRI GQFLFWHLRS EVHTPAVSIQ FGVILEAYCR GSVGHMKVLS KQVEALNKLK 730 740 750 760 770 780 TLNSLIKLNA MKLNRAKGKE AMHTCLKQSA YREALSDLQS PLNPCVILSE LYVEKCRYMD 790 800 810 820 830 840 SKMKPLWLVY SNRAFGEDAV GVIFKNGDDL RQDMLTLQML RLMDLLWKEA GLDLRMLPYG 850 860 870 880 890 900 CLATGDRSGL IEVVSTSETI ADIQLNSSNV AATAAFNKDA LLNWLKEYNS GDDLDRAIEE 910 920 930 940 950 960 FTLSCAGYCV ASYVLGIGDR HSDNIMVKKT GQLFHIDFGH ILGNFKSKFG IKRERVPFIL 970 980 990 1000 1010 1020 TYDFIHVIQQ GKTGNTEKFG RFRQCCEDAY LILRRHGNLF ITLFALMLTA GLPELTSVKD 1030 1040 1050 1060 1070 IQYLKDSLAL GKSEEEALKQ FKQKFDEALR ESWTTKVNWM AHTVRKDYRS

References

[1]	"Phosphatidylinositol-3 kinase and activation of phosphodiesterase 3B in adipocytes." Mulder H., Stenson Holst L., Degerman E. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ012482 mRNA. Translation: CAA10046.1.
IPI	IPI00208989.
RefSeq	NP_445933.1.
UniGene	Rn.44268
3D structure databases
SMR	Q9Z1L0. Positions 120-1065.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Z1L0.
PTM databases
PhosphoSite	Q9Z1L0.
Proteomic databases
PRIDE	Q9Z1L0.
Genome annotation databases
Ensembl	ENSRNOT00000022179; ENSRNOP00000022179; ENSRNOG00000016384; Rattus norvegicus. [Genome view]
GeneID	85243.
KEGG	rno:85243.
UCSC	NM_053481. rat.
Organism-specific databases
CTD	85243.
RGD	620917. Pik3cb.
Phylogenomic databases
eggNOG	roNOG11071.
HOVERGEN	Q9Z1L0.
InParanoid	Q9Z1L0.
PhylomeDB	Q9Z1L0.
Enzyme and pathway databases
BRENDA	2.7.1.153. 248.
Gene expression databases
Genevestigator	Q9Z1L0.
GermOnline	ENSRNOG00000016384. Rattus norvegicus.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR002420. PI3K_C2. IPR003113. PI3K_p85_bd. IPR000341. PI3K_ras_bd. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit. G3DSA:1.25.40.70. PI3Ka. 1 hit.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF02192. PI3K_p85B. 1 hit. PF00794. PI3K_rbd. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
SMART	SM00142. PI3K_C2. 1 hit. SM00143. PI3K_p85B. 1 hit. SM00144. PI3K_rbd. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	617306.

Entry information

Entry name

PK3CB_RAT

Accession

Primary (citable) accession number: Q9Z1L0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)