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Protein

Y+L amino acid transporter 1

Gene

Slc7a7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Plays a role in nitric oxide synthesis via transport of L-arginine, and is involved in the transport of L-arginine in monocytes.1 Publication

Kineticsi

  1. KM=91.5 µM for L-arginine (in the absence of NaCl)1 Publication
  2. KM=340.8 µM for L-arginine (in the presence of 0.1 M NaCl)1 Publication
  3. KM=21.7 µM for L-leucine (in the presence of 0.1 M NaCl)1 Publication
  4. KM=1.36 mM for L-alanine (in the presence of 0.1 M NaCl)1 Publication
  5. KM=47.9 µM for L-leucine (in the presence of 0.1 M LiCl)1 Publication

    GO - Molecular functioni

    • antiporter activity Source: GO_Central
    • basic amino acid transmembrane transporter activity Source: MGI
    • L-amino acid transmembrane transporter activity Source: GO_Central

    GO - Biological processi

    • basic amino acid transport Source: GOC
    • L-alpha-amino acid transmembrane transport Source: GOC
    • L-amino acid transport Source: GOC
    • regulation of arginine metabolic process Source: MGI
    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
    REACT_323105. Basigin interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Y+L amino acid transporter 1
    Alternative name(s):
    Solute carrier family 7 member 7
    y(+)L-type amino acid transporter 1
    Short name:
    Y+LAT1
    Short name:
    y+LAT-1
    Gene namesi
    Name:Slc7a7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 14

    Organism-specific databases

    MGIiMGI:1337120. Slc7a7.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei38 – 5821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei70 – 9021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei108 – 12821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei134 – 15421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei161 – 18121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei187 – 20721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei223 – 24321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei305 – 32521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei384 – 40421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei417 – 43721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei442 – 46221HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 510510Y+L amino acid transporter 1PRO_0000304935Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi3 – 31N-linked (GlcNAc...)Sequence Analysis
    Modified residuei19 – 191Phosphoserine2 Publications
    Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ9Z1K8.
    PRIDEiQ9Z1K8.

    PTM databases

    PhosphoSiteiQ9Z1K8.

    Expressioni

    Tissue specificityi

    Strongly expressed in kidney and intestine. Weaker expression observed in epididymis, testis, ovary, thyroid pancreas, sub-gland and liver.1 Publication

    Gene expression databases

    BgeeiQ9Z1K8.
    GenevisibleiQ9Z1K8. MM.

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.By similarity

    Protein-protein interaction databases

    BioGridi203319. 1 interaction.
    STRINGi10090.ENSMUSP00000000984.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z1K8.
    SMRiQ9Z1K8. Positions 46-373.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0531.
    GeneTreeiENSGT00760000119037.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ9Z1K8.
    KOiK13867.
    OMAiPIFPNCL.
    OrthoDBiEOG73BVCR.
    PhylomeDBiQ9Z1K8.
    TreeFamiTF313355.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9Z1K8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVNSTKYEVA AQHEADDGSA LGDGASPVAE QVKLKKEISL LNGVCLIVGN
    60 70 80 90 100
    MIGSGIFVSP KGVLMYSASF GLSLVIWAVG GIFSVFGALC YAELGTTIKK
    110 120 130 140 150
    SGASYAYILE AFGGFLAFIR LWTSLLIIEP TSQAVIAITF ANYMVQPLFP
    160 170 180 190 200
    SCGAPYAAGR LLAAACICLL TFINCAYVKW GTLVQDIFTY AKVLALIAVI
    210 220 230 240 250
    IAGIVRLGQG ATANFENSFE GSSFAMGDIA LALYSALFSY SGWDTLNYVT
    260 270 280 290 300
    EEIRNPERNL PLSIGISMPI VTIIYLLTNV AYYSVLDIKE ILASDAVAVT
    310 320 330 340 350
    FADQIFGVFN WIIPVAVAFS CFGGLNASIV AASRLLFVGS REGHLPDAIC
    360 370 380 390 400
    MVHVERFTPV PSLLFNGVLS LVYLCVEDIF QLINYYSFSY WFFVGLSIVG
    410 420 430 440 450
    QLYLRWKDPD RPRPLKLSLF FPIIFCLCTI FLVAVPLYSD TINSLIGIGI
    460 470 480 490 500
    ALSGLPFYFF IIRVPEHKRP LFLRRIVASI TRYLQILCMS VAAEMDLEDG
    510
    ELSKQDPKSK
    Length:510
    Mass (Da):55,677
    Last modified:May 1, 1999 - v1
    Checksum:i9F30FB1B88126F6C
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti4 – 41S → T.1 Publication

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ012754 mRNA. Translation: CAA10170.1.
    AJ130943 mRNA. Translation: CAA10255.1.
    AK150950 mRNA. Translation: BAE29983.1.
    AK151718 mRNA. Translation: BAE30637.1.
    AK152637 mRNA. Translation: BAE31379.1.
    AK170851 mRNA. Translation: BAE42073.1.
    BC014709 mRNA. Translation: AAH14709.1.
    CCDSiCCDS27087.1.
    RefSeqiNP_001240608.1. NM_001253679.1.
    NP_001240609.1. NM_001253680.1.
    NP_035535.2. NM_011405.4.
    XP_006518811.1. XM_006518748.2.
    XP_006518814.1. XM_006518751.2.
    XP_006518815.1. XM_006518752.1.
    XP_006518816.1. XM_006518753.1.
    XP_006518817.1. XM_006518754.2.
    XP_006518818.1. XM_006518755.2.
    UniGeneiMm.142455.
    Mm.489701.

    Genome annotation databases

    EnsembliENSMUST00000000984; ENSMUSP00000000984; ENSMUSG00000000958.
    ENSMUST00000195970; ENSMUSP00000143091; ENSMUSG00000000958.
    ENSMUST00000197440; ENSMUSP00000143743; ENSMUSG00000000958.
    GeneIDi20540.
    KEGGimmu:20540.
    UCSCiuc007tvv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ012754 mRNA. Translation: CAA10170.1.
    AJ130943 mRNA. Translation: CAA10255.1.
    AK150950 mRNA. Translation: BAE29983.1.
    AK151718 mRNA. Translation: BAE30637.1.
    AK152637 mRNA. Translation: BAE31379.1.
    AK170851 mRNA. Translation: BAE42073.1.
    BC014709 mRNA. Translation: AAH14709.1.
    CCDSiCCDS27087.1.
    RefSeqiNP_001240608.1. NM_001253679.1.
    NP_001240609.1. NM_001253680.1.
    NP_035535.2. NM_011405.4.
    XP_006518811.1. XM_006518748.2.
    XP_006518814.1. XM_006518751.2.
    XP_006518815.1. XM_006518752.1.
    XP_006518816.1. XM_006518753.1.
    XP_006518817.1. XM_006518754.2.
    XP_006518818.1. XM_006518755.2.
    UniGeneiMm.142455.
    Mm.489701.

    3D structure databases

    ProteinModelPortaliQ9Z1K8.
    SMRiQ9Z1K8. Positions 46-373.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi203319. 1 interaction.
    STRINGi10090.ENSMUSP00000000984.

    PTM databases

    PhosphoSiteiQ9Z1K8.

    Proteomic databases

    PaxDbiQ9Z1K8.
    PRIDEiQ9Z1K8.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000000984; ENSMUSP00000000984; ENSMUSG00000000958.
    ENSMUST00000195970; ENSMUSP00000143091; ENSMUSG00000000958.
    ENSMUST00000197440; ENSMUSP00000143743; ENSMUSG00000000958.
    GeneIDi20540.
    KEGGimmu:20540.
    UCSCiuc007tvv.2. mouse.

    Organism-specific databases

    CTDi9056.
    MGIiMGI:1337120. Slc7a7.

    Phylogenomic databases

    eggNOGiCOG0531.
    GeneTreeiENSGT00760000119037.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ9Z1K8.
    KOiK13867.
    OMAiPIFPNCL.
    OrthoDBiEOG73BVCR.
    PhylomeDBiQ9Z1K8.
    TreeFamiTF313355.

    Enzyme and pathway databases

    ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
    REACT_323105. Basigin interactions.

    Miscellaneous databases

    NextBioi298823.
    PROiQ9Z1K8.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ9Z1K8.
    GenevisibleiQ9Z1K8. MM.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Amino acid transport of y+L-type by heterodimers of 4F2hc/CD98 and members of the glycoprotein-associated amino acid transporter family."
      Pfeiffer R., Rossier G., Spindler B., Meier C., Kuehn L.C., Verrey F.
      EMBO J. 18:49-57(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY, VARIANT THR-4.
      Strain: BALB/c and NIH Swiss.
      Tissue: Heart and Kidney.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiYLAT1_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z1K8
    Secondary accession number(s): Q9QWS1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: May 1, 1999
    Last modified: July 22, 2015
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.