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Q9Z1K8 (YLAT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Y+L amino acid transporter 1
Alternative name(s):
Solute carrier family 7 member 7
y(+)L-type amino acid transporter 1
Short name=Y+LAT1
Short name=y+LAT-1
Gene names
Name:Slc7a7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length510 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the sodium-independent uptake of dibasic amino acids and sodium-dependent uptake of some neutral amino acids. Requires coexpression with SLC3A2/4F2hc to mediate the uptake of arginine, leucine and glutamine. Plays a role in nitric oxide synthesis via transport of L-arginine, and is involved in the transport of L-arginine in monocytes. Ref.1

Subunit structure

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc By similarity. Ref.1

Subcellular location

Basolateral cell membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Strongly expressed in kidney and intestine. Weaker expression observed in epididymis, testis, ovary, thyroid pancreas, sub-gland and liver. Ref.1

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=91.5 µM for L-arginine (in the absence of NaCl) Ref.1

KM=340.8 µM for L-arginine (in the presence of 0.1 M NaCl)

KM=21.7 µM for L-leucine (in the presence of 0.1 M NaCl)

KM=1.36 mM for L-alanine (in the presence of 0.1 M NaCl)

KM=47.9 µM for L-leucine (in the presence of 0.1 M LiCl)

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 510510Y+L amino acid transporter 1
PRO_0000304935

Regions

Transmembrane38 – 5821Helical; Potential
Transmembrane70 – 9021Helical; Potential
Transmembrane108 – 12821Helical; Potential
Transmembrane134 – 15421Helical; Potential
Transmembrane161 – 18121Helical; Potential
Transmembrane187 – 20721Helical; Potential
Transmembrane223 – 24321Helical; Potential
Transmembrane260 – 28021Helical; Potential
Transmembrane305 – 32521Helical; Potential
Transmembrane384 – 40421Helical; Potential
Transmembrane417 – 43721Helical; Potential
Transmembrane442 – 46221Helical; Potential

Amino acid modifications

Modified residue191Phosphoserine Ref.4 Ref.5
Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation3261N-linked (GlcNAc...) Potential

Natural variations

Natural variant41S → T. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9Z1K8 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 9F30FB1B88126F6C

FASTA51055,677
        10         20         30         40         50         60 
MVNSTKYEVA AQHEADDGSA LGDGASPVAE QVKLKKEISL LNGVCLIVGN MIGSGIFVSP 

        70         80         90        100        110        120 
KGVLMYSASF GLSLVIWAVG GIFSVFGALC YAELGTTIKK SGASYAYILE AFGGFLAFIR 

       130        140        150        160        170        180 
LWTSLLIIEP TSQAVIAITF ANYMVQPLFP SCGAPYAAGR LLAAACICLL TFINCAYVKW 

       190        200        210        220        230        240 
GTLVQDIFTY AKVLALIAVI IAGIVRLGQG ATANFENSFE GSSFAMGDIA LALYSALFSY 

       250        260        270        280        290        300 
SGWDTLNYVT EEIRNPERNL PLSIGISMPI VTIIYLLTNV AYYSVLDIKE ILASDAVAVT 

       310        320        330        340        350        360 
FADQIFGVFN WIIPVAVAFS CFGGLNASIV AASRLLFVGS REGHLPDAIC MVHVERFTPV 

       370        380        390        400        410        420 
PSLLFNGVLS LVYLCVEDIF QLINYYSFSY WFFVGLSIVG QLYLRWKDPD RPRPLKLSLF 

       430        440        450        460        470        480 
FPIIFCLCTI FLVAVPLYSD TINSLIGIGI ALSGLPFYFF IIRVPEHKRP LFLRRIVASI 

       490        500        510 
TRYLQILCMS VAAEMDLEDG ELSKQDPKSK 

« Hide

References

« Hide 'large scale' references
[1]"Amino acid transport of y+L-type by heterodimers of 4F2hc/CD98 and members of the glycoprotein-associated amino acid transporter family."
Pfeiffer R., Rossier G., Spindler B., Meier C., Kuehn L.C., Verrey F.
EMBO J. 18:49-57(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY, VARIANT THR-4.
Strain: BALB/c and NIH Swiss.
Tissue: Heart and Kidney.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Bone marrow.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[5]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ012754 mRNA. Translation: CAA10170.1.
AJ130943 mRNA. Translation: CAA10255.1.
AK150950 mRNA. Translation: BAE29983.1.
AK151718 mRNA. Translation: BAE30637.1.
AK152637 mRNA. Translation: BAE31379.1.
AK170851 mRNA. Translation: BAE42073.1.
BC014709 mRNA. Translation: AAH14709.1.
RefSeqNP_001240608.1. NM_001253679.1.
NP_001240609.1. NM_001253680.1.
NP_035535.2. NM_011405.4.
XP_006518811.1. XM_006518748.1.
XP_006518812.1. XM_006518749.1.
XP_006518813.1. XM_006518750.1.
XP_006518814.1. XM_006518751.1.
XP_006518815.1. XM_006518752.1.
XP_006518816.1. XM_006518753.1.
XP_006518817.1. XM_006518754.1.
XP_006518818.1. XM_006518755.1.
UniGeneMm.142455.
Mm.489701.

3D structure databases

ProteinModelPortalQ9Z1K8.
SMRQ9Z1K8. Positions 46-373.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203319. 1 interaction.

PTM databases

PhosphoSiteQ9Z1K8.

Proteomic databases

PaxDbQ9Z1K8.
PRIDEQ9Z1K8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000984; ENSMUSP00000000984; ENSMUSG00000000958.
GeneID20540.
KEGGmmu:20540.
UCSCuc007tvv.2. mouse.

Organism-specific databases

CTD9056.
MGIMGI:1337120. Slc7a7.

Phylogenomic databases

eggNOGCOG0531.
GeneTreeENSGT00730000110280.
HOGENOMHOG000098892.
HOVERGENHBG000476.
InParanoidQ9Z1K8.
KOK13867.
OMANSSIMAS.
OrthoDBEOG73BVCR.
TreeFamTF313355.

Gene expression databases

BgeeQ9Z1K8.
GenevestigatorQ9Z1K8.

Family and domain databases

InterProIPR002293. AA/rel_permease1.
[Graphical view]
PANTHERPTHR11785. PTHR11785. 1 hit.
PfamPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
ProtoNetSearch...

Other

NextBio298823.
PROQ9Z1K8.
SOURCESearch...

Entry information

Entry nameYLAT1_MOUSE
AccessionPrimary (citable) accession number: Q9Z1K8
Secondary accession number(s): Q9QWS1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: May 1, 1999
Last modified: March 19, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot