Q9Z1K7 (APC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenomatous polyposis coli protein 2

Gene names

Name:

Apc2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	2274 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes rapid degradation of CTNNB1 and may function as a tumor suppressor. May function in Wnt signaling By similarity.
Subunit structure	Interacts with APC, CTNNB1, TP53BP2 and possibly with AXIN2 By similarity. Interacts with MAPRE3, PSRC1 and probably MAPRE1. Ref.3
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm › cytoskeleton By similarity. Note: Associated with actin filaments and the microtubule network By similarity.
Tissue specificity	Expressed in brain and other neural tissues. Ref.2
Developmental stage	Strongly expressed in fetal brain but expression decreases from P12 onward. Expressed in post-mitotic neurons. Ref.2
Sequence similarities	Belongs to the adenomatous polyposis coli (APC) family. Contains 6 ARM repeats.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cell membrane Cytoplasm Cytoskeleton Golgi apparatus Membrane Microtubule
Domain	Coiled coil Repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2274	2274	Adenomatous polyposis coli protein 2		PRO_0000313687
Regions
	Repeat	301 – 341	41	ARM 1
	Repeat	472 – 511	40	ARM 2
	Repeat	515 – 555	41	ARM 3
	Repeat	557 – 602	46	ARM 4
	Repeat	608 – 647	40	ARM 5
	Repeat	650 – 689	40	ARM 6
	Repeat	1049 – 1068	20	1
	Repeat	1140 – 1159	20	2
	Repeat	1250 – 1269	20	3
	Repeat	1375 – 1394	20	4
	Repeat	1546 – 1565	20	5
	Region	1049 – 1565	517	5 X 20 AA approximate repeat of F-X-V-E-X-T-P-X-C-F-S-R-X-S-S-L-S-S-L-S
	Region	1049 – 1565	517	Interaction with CTNNB1 By similarity
	Region	2037 – 2114	78	Interaction with MAPRE1 and MAPRE3 By similarity
	Coiled coil	5 – 59	55	Potential
	Coiled coil	832 – 856	25	Potential
	Compositional bias	1057 – 1186	130	Ser-rich
	Compositional bias	1829 – 1922	94	Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	Q9Z1K7 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 75ABDA15D0F707F5	FASTA	2,274	243,139
	10 20 30 40 50 60 MTSSMASYEQ LVRQVEALKA ENTHLRQELR DNSSHLSKLE TETSGMKEVL KHLQGKLEQE 70 80 90 100 110 120 ARVLVSSGQT EVLEQLKALQ TDISSLYNLK FHAPALGPEP AARTPEGSPV HGSGPSKDSF 130 140 150 160 170 180 GELSRATIRL LEELDQERCF LLSEIEKEEK EKLWYYSQLQ GLSKRLDELP HVDTFSMQMD 190 200 210 220 230 240 LIRQQLEFEA QHIRSLMEER FGTSDEMVQR AQIRASRLEQ IDKELLEAQD RVQQTEPQAL 250 260 270 280 290 300 LAVKPVAVEE EQEAEVPTHP EDGTPQPGNS KVEVVFWLLS MLATRDQEDT ARTLLAMSSS 310 320 330 340 350 360 PESCVAMRRS GCLPLLLQIL HGTEAGSVGR AGIPGAPGAK DARMRANAAL HNIVFSQPDQ 370 380 390 400 410 420 GLARKEMRVL HVLEQIRAYC ETCWDWLQAR DSGTETPVPI EPQICQATCA VMKLSFDEEY 430 440 450 460 470 480 RRAMNELGGL QAVAELLQVD YEMHKMTRDP LNLALRRYAG MTLTNLTFGD VANKATLCAR 490 500 510 520 530 540 RGCMEAIVAQ LGSESEELHQ VVSSILRNLS WRADINSKKV LREVGSMTAL MECVLRASKE 550 560 570 580 590 600 STLKSVLSAL WNLSAHSTEN KAAICQVDGA LGFLVSTLTY RCQGNSLAVI ESGGGILRNV 610 620 630 640 650 660 SSLIATREDY RQVLRDHNCL QTLLQHLTSH SLTIVSNACG TLWNLSARSP RDQELLWDLG 670 680 690 700 710 720 AVGMLRNLVH SKHKMIAMGS AAALRNLLAH RPAKYQAAAM AVSPGTCVPS LYVRKQRALE 730 740 750 760 770 780 AELDTRHLVH ALGHLEKQSL PEAETTSKKP LPPLRHLDGL VQDYASDSGC FDDDDAPSLA 790 800 810 820 830 840 AAATTAEPAS PAVMSMFLGG PFLQGQALAR TPPARQGGLE AEKEAGGEAA VAAKAKAKLA 850 860 870 880 890 900 LAVARIDRLV EDISALHTSS DDSFSLSSGD PGQEAPREGR AQSCSPCRGT EGGRREAGSR 910 920 930 940 950 960 AHPLLRLKAA HTSLSNDSLN SGSTSDGYCT REHMTPCPLA ALAEHRDDPV RGQTRPRRLD 970 980 990 1000 1010 1020 LDLPSRAELP ARDTAATDAR VRTIKLSPTY QHVPLLDGAA GAGVRPLVGP GTSPGARKQA 1030 1040 1050 1060 1070 1080 WIPADSLSKV PEKLVASPLP IASKVLQKLV AQDGPMSLSR CSSLSSLSST GHAVPSQAEN 1090 1100 1110 1120 1130 1140 LDSDSSLEGL EEAGPGEAEL GRAWRASGST SLPVSIPAPQ RGRSRGLGVE DATPSSSSEN 1150 1160 1170 1180 1190 1200 CVQETPLVLS RCSSVSSLGS FESRSIASSI PSDPCSGLGS GTVSPSELPD SPGQTMPPSR 1210 1220 1230 1240 1250 1260 SKTPPAPPGQ PETSQFSLQW ESYVKRFLDI ADCRERCQPP SELDAGSVRF TVEKPDENFS 1270 1280 1290 1300 1310 1320 CASSLSALAL HELYVQQDVE LRLRPPACPE RAVGGGGHRR RDEAASRLDG PAPAGSRARS 1330 1340 1350 1360 1370 1380 ATDKELEALR ECLGAAMPAR LRKVASALVP GRRSLPVPVY MLVPAPARGD DSGTDSAEGT 1390 1400 1410 1420 1430 1440 PVNFSSAASL SDETLQGPSR DKPAGPGDRQ KPTGRAAPAR QTRSHRPKAA GAGKSTEHTR 1450 1460 1470 1480 1490 1500 GPCRNRAGLE LPLSRPQSAR SNRDSSCQTR TRGDGALQSL CLTTPTEEAV YCFYDSDEEP 1510 1520 1530 1540 1550 1560 PATAPPPRRA SAIPRALKRE KPAGRKETPS RAAQPATLPV RAQPRLIVDE TPPCYSLTSS 1570 1580 1590 1600 1610 1620 ASSLSEPEAP EQPANHARGP EQGSKQDSSP SPRAEEELLQ RCISLAMPRR RTQVPGSRRR 1630 1640 1650 1660 1670 1680 KPRALRSDIR PTEITQKCQE EVAGSDPASD LDSVEWQAIQ EGANSIVTWL HQAAAKASLE 1690 1700 1710 1720 1730 1740 ASSESDSLLS LVSGVSAGST LQPSKLRKGR KPAAEAGGAW RPEKRGTTST KINGSPRLPN 1750 1760 1770 1780 1790 1800 GPEKAKGTQK MMAGESTMLR GRTVIYSAGP ASRTQSKGIS GPCTTPKKTG TSGTTQPETV 1810 1820 1830 1840 1850 1860 TKAPSPEQQR SRSLHRPGKI SELAALRHPP RSATPPARLA KTPSSSSSQT SPASQPLPRR 1870 1880 1890 1900 1910 1920 SPLATPTGGP LPGPGGSLVP KSPARALLAK QHKTQKSPVR IPFMQRPARR VPPPLARPSP 1930 1940 1950 1960 1970 1980 EPGSRGRAGA EGTPGARGSR LGLVRMASAR SSGSESSDRS GFRRQLTFIK ESPGLLRRRR 1990 2000 2010 2020 2030 2040 SELSSADSTA STSQAASPRR GRPALPAVFL CSSRCDELRV SPRQPLAAQR SPQAKPGLAP 2050 2060 2070 2080 2090 2100 LAPRRTSSES PSRLPVRASP GRPETVKRYA SLPHISVSRR SDSAVSVPTT QANATRRGSD 2110 2120 2130 2140 2150 2160 GEARPLPRVA PPGTTWRRIK DEDVPHILRS TLPATALPLR VSSPEDSPAG TPQRKTSDAV 2170 2180 2190 2200 2210 2220 VQTEDVATSK TNSSTSPSLE SRDPPQAPAS GPVAPQGSDV DGPVLTKPPA SAPFPHEGLS 2230 2240 2250 2260 2270 AVIAGFPTSR HGSPSRAARV PPFNYVPSPM AAATMASDSA VEKAPVSSPA SLLE

References

[1]	"Identification of APC2, a homologue of the adenomatous polyposis coli tumour suppressor." van Es J.H., Kirkpatrick C., van de Wetering M., Molenaar M., Miles A., Kuipers J., Destree O., Peifer M., Clevers H. Curr. Biol. 9:105-108(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Expression of Apc2 during mouse development." Yamanaka H., Hashimoto N., Koyama K., Nakagawa H., Nakamura Y., Noguchi K. Gene Expr. Patterns 1:107-114(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"p53 downstream target DDA3 is a novel microtubule-associated protein that interacts with end-binding protein EB3 and activates beta-catenin pathway." Hsieh P.-C., Chang J.-C., Sun W.-T., Hsieh S.-C., Wang M.-C., Wang F.-F. Oncogene 26:4928-4940(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PSRC1 AND MAPRE3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ130783 , AJ130784, AJ130785, AJ130786, AJ130787, AJ130788, AJ130789, AJ130790, AJ130791, AJ130792, AJ130793, AJ130794, AJ130795, AJ130796 Genomic DNA. Translation: CAA10207.1.
IPI	IPI00130238.
PIR	T30258.
UniGene	Mm.57247.
3D structure databases
HSSP	HSSP built from PDB template 1DEB based on UniProtKB P25054.
ProteinModelPortal	Q9Z1K7.
SMR	Q9Z1K7. Positions 6-57, 131-233, 286-695.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000100996.
PTM databases
PhosphoSite	Q9Z1K7.
Proteomic databases
PaxDb	Q9Z1K7.
PRIDE	Q9Z1K7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:1346052. Apc2.
Phylogenomic databases
eggNOG	NOG259696.
HOGENOM	HOG000033986.
HOVERGEN	HBG104821.
Gene expression databases
CleanEx	MM_APC2.
Genevestigator	Q9Z1K7.
Family and domain databases
Gene3D	1.25.10.10. 2 hits.
InterPro	IPR026837. APC2. IPR009234. APC_basic_dom. IPR009223. APC_Cys-rich_rpt. IPR026831. APC_dom. IPR026818. Apc_fam. IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. IPR009224. SAMP. [Graphical view]
PANTHER	PTHR12607. PTHR12607. 1 hit. PTHR12607:SF3. PTHR12607:SF3. 1 hit.
Pfam	PF05956. APC_basic. 1 hit. PF05923. APC_crr. 4 hits. PF00514. Arm. 1 hit. PF05924. SAMP. 2 hits. PF11414. Suppressor_APC. 1 hit. [Graphical view]
SMART	SM00185. ARM. 6 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50176. ARM_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

APC2_MOUSE

Accession

Primary (citable) accession number: Q9Z1K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program