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Protein

E3 ubiquitin-protein ligase ARIH2

Gene

Arih2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase mediating 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins. May play a role in myelopoiesis (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri138 – 18750RING-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri207 – 26963IBR-typeAdd
BLAST
Zinc fingeri296 – 32530RING-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase ARIH2 (EC:6.3.2.-)
Short name:
ARI-2
Short name:
Protein ariadne-2 homolog
Alternative name(s):
Triad1 protein
UbcM4-interacting protein 48
Gene namesi
Name:Arih2
Synonyms:Ari2, Triad1, Uip48
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1344361. Arih2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 492492E3 ubiquitin-protein ligase ARIH2PRO_0000055756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei352 – 3521PhosphoserineCombined sources

Post-translational modificationi

Ubiquitinated. Ubiquitination promotes proteasomal degradation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Z1K6.
MaxQBiQ9Z1K6.
PaxDbiQ9Z1K6.
PRIDEiQ9Z1K6.

PTM databases

iPTMnetiQ9Z1K6.
PhosphoSiteiQ9Z1K6.

Expressioni

Gene expression databases

BgeeiQ9Z1K6.
CleanExiMM_ARIH2.
ExpressionAtlasiQ9Z1K6. baseline and differential.
GenevisibleiQ9Z1K6. MM.

Interactioni

Subunit structurei

Interacts (via RING-type 2) with UBE2N. Interacts (via RING-type 2) with GFI1B. Interacts with GFI1; prevents its ubiquitination and proteasomal degradation (By similarity). Interacts (via RING-type 1) with UBE2L3.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
NfkbibQ607782EBI-6861719,EBI-644469

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204727. 4 interactions.
IntActiQ9Z1K6. 1 interaction.
STRINGi10090.ENSMUSP00000013338.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1K6.
SMRiQ9Z1K6. Positions 60-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili368 – 39932Sequence analysisAdd
BLAST
Coiled coili438 – 49154Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 7471Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi22 – 298Poly-Glu

Domaini

RING-type 1 and RING-type 2 are required for the inhibitory function in myelopoiesis.By similarity

Sequence similaritiesi

Belongs to the RBR family. Ariadne subfamily.Curated
Contains 1 IBR-type zinc finger.Curated
Contains 2 RING-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri138 – 18750RING-type 1; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri207 – 26963IBR-typeAdd
BLAST
Zinc fingeri296 – 32530RING-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129790.
HOGENOMiHOG000216611.
HOVERGENiHBG018737.
InParanoidiQ9Z1K6.
KOiK11969.
OMAiFRDYVEX.
OrthoDBiEOG7CZK58.
PhylomeDBiQ9Z1K6.
TreeFamiTF300805.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1K6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVDMNSQGS DSNEEDYDPN CEEEEEEEED PGDIEDYYVG VASDVEQQGA
60 70 80 90 100
DAFDPEEYQF TCLTYKESEG ALHEHMTSLA SVLKVSHSVA KLILVNFHWQ
110 120 130 140 150
VSEILDRYRS NSAQLLVEAR VQPNPSKHVP TAHPPHHCAV CMQFVRKENL
160 170 180 190 200
LSLACQHQFC RSCWEQHCSV LVKDGVGVGI SCMAQDCPLR TPEDFVFPLL
210 220 230 240 250
PNEELRDKYR RYLFRDYVES HFQLQLCPGA DCPMVIRVQE PRARRVQCNR
260 270 280 290 300
CSEVFCFKCR QMYHAPTDCA TIRKWLTKCA DDSETANYIS AHTKDCPKCN
310 320 330 340 350
ICIEKNGGCN HMQCSKCKHD FCWMCLGDWK THGSEYYECS RYKENPDIVN
360 370 380 390 400
QSQQAQAREA LKKYLFYFER WENHNKSLQL EAQTYERIHE KIQERVMNNL
410 420 430 440 450
GTWIDWQYLQ NAAKLLAKCR YTLQYTYPYA YYMESGPRKK LFEYQQAQLE
460 470 480 490
AEIENLSWKV ERADSYDRGD LENQMHIAEQ RRRTLLKDFH DT
Length:492
Mass (Da):57,697
Last modified:May 1, 1999 - v1
Checksum:iB55EA54FE8C3ADAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124664 mRNA. Translation: AAD24573.1.
AJ130975 mRNA. Translation: CAA10273.1.
BC051998 mRNA. Translation: AAH51998.1.
BC052422 mRNA. Translation: AAH52422.1.
CCDSiCCDS23534.1.
RefSeqiNP_035920.1. NM_011790.4.
XP_006511788.1. XM_006511725.2.
XP_006511789.1. XM_006511726.1.
UniGeneiMm.290447.

Genome annotation databases

EnsembliENSMUST00000013338; ENSMUSP00000013338; ENSMUSG00000064145.
GeneIDi23807.
KEGGimmu:23807.
UCSCiuc009rqo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124664 mRNA. Translation: AAD24573.1.
AJ130975 mRNA. Translation: CAA10273.1.
BC051998 mRNA. Translation: AAH51998.1.
BC052422 mRNA. Translation: AAH52422.1.
CCDSiCCDS23534.1.
RefSeqiNP_035920.1. NM_011790.4.
XP_006511788.1. XM_006511725.2.
XP_006511789.1. XM_006511726.1.
UniGeneiMm.290447.

3D structure databases

ProteinModelPortaliQ9Z1K6.
SMRiQ9Z1K6. Positions 60-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204727. 4 interactions.
IntActiQ9Z1K6. 1 interaction.
STRINGi10090.ENSMUSP00000013338.

PTM databases

iPTMnetiQ9Z1K6.
PhosphoSiteiQ9Z1K6.

Proteomic databases

EPDiQ9Z1K6.
MaxQBiQ9Z1K6.
PaxDbiQ9Z1K6.
PRIDEiQ9Z1K6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000013338; ENSMUSP00000013338; ENSMUSG00000064145.
GeneIDi23807.
KEGGimmu:23807.
UCSCiuc009rqo.1. mouse.

Organism-specific databases

CTDi10425.
MGIiMGI:1344361. Arih2.

Phylogenomic databases

eggNOGiKOG1815. Eukaryota.
ENOG410XP9Y. LUCA.
GeneTreeiENSGT00840000129790.
HOGENOMiHOG000216611.
HOVERGENiHBG018737.
InParanoidiQ9Z1K6.
KOiK11969.
OMAiFRDYVEX.
OrthoDBiEOG7CZK58.
PhylomeDBiQ9Z1K6.
TreeFamiTF300805.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiQ9Z1K6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z1K6.
CleanExiMM_ARIH2.
ExpressionAtlasiQ9Z1K6. baseline and differential.
GenevisibleiQ9Z1K6. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR031127. E3_UB_ligase_RBR.
IPR002867. IBR_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR11685. PTHR11685. 1 hit.
PfamiPF01485. IBR. 2 hits.
[Graphical view]
SMARTiSM00647. IBR. 2 hits.
SM00184. RING. 2 hits.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A family of structurally related RING finger proteins interacts specifically with the ubiquitin-conjugating enzyme UbcM4."
    Martinez-Noel G., Niedenthal R., Tamura T., Harbers K.
    FEBS Lett. 454:257-261(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
    Tissue: Liver.
  2. "Ariadne-1: a vital Drosophila gene is required in development and defines a new conserved family of ring-finger proteins."
    Aguilera M., Oliveros M., Martinez-Padron M., Barbas J.A., Ferrus A.
    Genetics 155:1231-1244(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain and Embryo.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-352, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiARI2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1K6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 1999
Last modified: July 6, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.