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Protein

Cysteine desulfurase, mitochondrial

Gene

Nfs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor (By similarity).By similarity

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.

Cofactori

pyridoxal 5'-phosphateBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei209 – 2091Pyridoxal phosphateBy similarity
Binding sitei237 – 2371Pyridoxal phosphateBy similarity
Binding sitei297 – 2971Pyridoxal phosphateBy similarity
Active sitei383 – 3831Cysteine persulfide intermediateBy similarity
Metal bindingi383 – 3831Iron-sulfur (2Fe-2S); via persulfide group; shared with ISCUBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.8.1.7. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase, mitochondrial (EC:2.8.1.7)
Short name:
m-Nfs1
Gene namesi
Name:Nfs1
Synonyms:Nifs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1316706. Nfs1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrial matrix Source: MGI
  • mitochondrion Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1717MitochondrionSequence analysisAdd
BLAST
Chaini18 – 459442Cysteine desulfurase, mitochondrialPRO_0000001294Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei260 – 2601N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

EPDiQ9Z1J3.
MaxQBiQ9Z1J3.
PaxDbiQ9Z1J3.
PRIDEiQ9Z1J3.

2D gel databases

REPRODUCTION-2DPAGEQ9Z1J3.

PTM databases

iPTMnetiQ9Z1J3.
PhosphoSiteiQ9Z1J3.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

CleanExiMM_NFS1.
ExpressionAtlasiQ9Z1J3. baseline and differential.
GenevisibleiQ9Z1J3. MM.

Interactioni

Subunit structurei

Binds ISCU/NIFUN. Forms a complex with LYRM4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9Z1J3. 1 interaction.
STRINGi10090.ENSMUSP00000029147.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1J3.
SMRiQ9Z1J3. Positions 60-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni129 – 1302Pyridoxal phosphate bindingBy similarity
Regioni257 – 2593Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1549. Eukaryota.
COG1104. LUCA.
GeneTreeiENSGT00530000063513.
HOGENOMiHOG000017510.
HOVERGENiHBG003708.
InParanoidiQ9Z1J3.
KOiK04487.
OMAiEPIQSGG.
PhylomeDBiQ9Z1J3.
TreeFamiTF105658.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR010240. Cys_deSase_IscS.
IPR016454. Cysteine_dSase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02006. IscS. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: Q9Z1J3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGSVAGNML LRAAWRRASL AATSLALGRS SVPTRGLRLR VVDHGPHSPV
60 70 80 90 100
HSEAEAVLRP LYMDVQATTP LDPRVLDAML PYLVNYYGNP HSRTHAYGWE
110 120 130 140 150
SEAAMERARQ QVASLIGADP REIIFTSGAT ESNNIAIKGV ARFYRSRKKH
160 170 180 190 200
LVTTQTEHKC VLDSCRSLEA EGFRVTYLPV QKSGIIDLKE LEAAIQPDTS
210 220 230 240 250
LVSVMTVNNE IGVKQPIAEI GQICSSRKVY FHTDAAQAVG KIPLDVNDMK
260 270 280 290 300
IDLMSISGHK LYGPKGVGAI YIRRRPRVRV EALQSGGGQE RGMRSGTVPT
310 320 330 340 350
PLVVGLGAAC ELAQQEMEYD HKRISKLAER LVQNIMKNLP DVVMNGDPKQ
360 370 380 390 400
HYPGCINLSF AYVEGESLLM ALKDVALSSG SACTSASLEP SYVLRAIGTD
410 420 430 440 450
EDLAHSSIRF GIGRFTTEEE VDYTAEKCIH HVKRLREMSP LWEMVQDGID

LKSIKWTQH
Length:459
Mass (Da):50,570
Last modified:September 18, 2013 - v3
Checksum:i986D636C5506C7B0
GO
Isoform Cytoplasmic (identifier: Q9Z1J3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.

Note: No experimental confirmation available.
Show »
Length:397
Mass (Da):43,972
Checksum:iCB0F1E7025E4E050
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti221 – 2211G → R in CAA10916 (PubMed:9738949).Curated
Sequence conflicti334 – 3341N → K in CAA10916 (PubMed:9738949).Curated
Sequence conflicti394 – 3941L → F in CAA10916 (PubMed:9738949).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6262Missing in isoform Cytoplasmic. 1 PublicationVSP_018647Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222660 mRNA. Translation: CAA10916.1.
AK075575 mRNA. Translation: BAC35831.1.
AK132838 mRNA. Translation: BAE21386.1.
AK148527 mRNA. Translation: BAE28601.1.
AK159828 mRNA. Translation: BAE35407.1.
AL833786 Genomic DNA. No translation available.
BX649640 Genomic DNA. No translation available.
CH466551 Genomic DNA. Translation: EDL06179.1.
CCDSiCCDS16962.1. [Q9Z1J3-1]
RefSeqiNP_035041.2. NM_010911.2. [Q9Z1J3-1]
UniGeneiMm.283604.

Genome annotation databases

EnsembliENSMUST00000029147; ENSMUSP00000029147; ENSMUSG00000027618. [Q9Z1J3-1]
GeneIDi18041.
KEGGimmu:18041.
UCSCiuc008nmj.2. mouse. [Q9Z1J3-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222660 mRNA. Translation: CAA10916.1.
AK075575 mRNA. Translation: BAC35831.1.
AK132838 mRNA. Translation: BAE21386.1.
AK148527 mRNA. Translation: BAE28601.1.
AK159828 mRNA. Translation: BAE35407.1.
AL833786 Genomic DNA. No translation available.
BX649640 Genomic DNA. No translation available.
CH466551 Genomic DNA. Translation: EDL06179.1.
CCDSiCCDS16962.1. [Q9Z1J3-1]
RefSeqiNP_035041.2. NM_010911.2. [Q9Z1J3-1]
UniGeneiMm.283604.

3D structure databases

ProteinModelPortaliQ9Z1J3.
SMRiQ9Z1J3. Positions 60-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z1J3. 1 interaction.
STRINGi10090.ENSMUSP00000029147.

PTM databases

iPTMnetiQ9Z1J3.
PhosphoSiteiQ9Z1J3.

2D gel databases

REPRODUCTION-2DPAGEQ9Z1J3.

Proteomic databases

EPDiQ9Z1J3.
MaxQBiQ9Z1J3.
PaxDbiQ9Z1J3.
PRIDEiQ9Z1J3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029147; ENSMUSP00000029147; ENSMUSG00000027618. [Q9Z1J3-1]
GeneIDi18041.
KEGGimmu:18041.
UCSCiuc008nmj.2. mouse. [Q9Z1J3-1]

Organism-specific databases

CTDi9054.
MGIiMGI:1316706. Nfs1.

Phylogenomic databases

eggNOGiKOG1549. Eukaryota.
COG1104. LUCA.
GeneTreeiENSGT00530000063513.
HOGENOMiHOG000017510.
HOVERGENiHBG003708.
InParanoidiQ9Z1J3.
KOiK04487.
OMAiEPIQSGG.
PhylomeDBiQ9Z1J3.
TreeFamiTF105658.

Enzyme and pathway databases

BRENDAi2.8.1.7. 3474.

Miscellaneous databases

ChiTaRSiNfs1. mouse.
PROiQ9Z1J3.
SOURCEiSearch...

Gene expression databases

CleanExiMM_NFS1.
ExpressionAtlasiQ9Z1J3. baseline and differential.
GenevisibleiQ9Z1J3. MM.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR010240. Cys_deSase_IscS.
IPR016454. Cysteine_dSase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02006. IscS. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and characterization of mouse nifS-like protein, m-Nfs1: mitochondrial localization of eukaryotic NifS-like proteins."
    Nakai Y., Yoshihara Y., Hayashi H., Kagamiyama H.
    FEBS Lett. 433:143-148(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MITOCHONDRIAL AND CYTOPLASMIC).
    Strain: BALB/cJ.
    Tissue: Olfactory epithelium.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM MITOCHONDRIAL).
    Strain: C57BL/6J.
    Tissue: Kidney, Pancreas and Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiNFS1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1J3
Secondary accession number(s): Q8C6I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: September 18, 2013
Last modified: June 8, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.