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Protein

Cysteine desulfurase, mitochondrial

Gene

Nfs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor (By similarity).By similarity

Catalytic activityi

L-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor.

Cofactori

pyridoxal 5'-phosphateBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei209Pyridoxal phosphateBy similarity1
Binding sitei237Pyridoxal phosphateBy similarity1
Binding sitei297Pyridoxal phosphateBy similarity1
Active sitei383Cysteine persulfide intermediateBy similarity1
Metal bindingi383Iron-sulfur (2Fe-2S); via persulfide group; shared with ISCUBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.8.1.7. 3474.
ReactomeiR-MMU-1362409. Mitochondrial iron-sulfur cluster biogenesis.
R-MMU-947581. Molybdenum cofactor biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine desulfurase, mitochondrial (EC:2.8.1.7)
Short name:
m-Nfs1
Gene namesi
Name:Nfs1
Synonyms:Nifs
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1316706. Nfs1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • mitochondrial matrix Source: MGI
  • mitochondrion Source: MGI
  • nucleolus Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 17MitochondrionSequence analysisAdd BLAST17
ChainiPRO_000000129418 – 459Cysteine desulfurase, mitochondrialAdd BLAST442

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei260N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9Z1J3.
PeptideAtlasiQ9Z1J3.
PRIDEiQ9Z1J3.

2D gel databases

REPRODUCTION-2DPAGEQ9Z1J3.

PTM databases

iPTMnetiQ9Z1J3.
PhosphoSitePlusiQ9Z1J3.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSMUSG00000027618.
CleanExiMM_NFS1.
ExpressionAtlasiQ9Z1J3. baseline and differential.
GenevisibleiQ9Z1J3. MM.

Interactioni

Subunit structurei

Binds ISCU/NIFUN. Forms a complex with LYRM4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9Z1J3. 1 interactor.
STRINGi10090.ENSMUSP00000029147.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1J3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni129 – 130Pyridoxal phosphate bindingBy similarity2
Regioni257 – 259Pyridoxal phosphate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1549. Eukaryota.
COG1104. LUCA.
GeneTreeiENSGT00530000063513.
HOGENOMiHOG000017510.
HOVERGENiHBG003708.
InParanoidiQ9Z1J3.
KOiK04487.
OMAiEPIQSGG.
OrthoDBiEOG091G072R.
PhylomeDBiQ9Z1J3.
TreeFamiTF105658.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR010240. Cys_deSase_IscS.
IPR016454. Cysteine_dSase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02006. IscS. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Mitochondrial (identifier: Q9Z1J3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGSVAGNML LRAAWRRASL AATSLALGRS SVPTRGLRLR VVDHGPHSPV
60 70 80 90 100
HSEAEAVLRP LYMDVQATTP LDPRVLDAML PYLVNYYGNP HSRTHAYGWE
110 120 130 140 150
SEAAMERARQ QVASLIGADP REIIFTSGAT ESNNIAIKGV ARFYRSRKKH
160 170 180 190 200
LVTTQTEHKC VLDSCRSLEA EGFRVTYLPV QKSGIIDLKE LEAAIQPDTS
210 220 230 240 250
LVSVMTVNNE IGVKQPIAEI GQICSSRKVY FHTDAAQAVG KIPLDVNDMK
260 270 280 290 300
IDLMSISGHK LYGPKGVGAI YIRRRPRVRV EALQSGGGQE RGMRSGTVPT
310 320 330 340 350
PLVVGLGAAC ELAQQEMEYD HKRISKLAER LVQNIMKNLP DVVMNGDPKQ
360 370 380 390 400
HYPGCINLSF AYVEGESLLM ALKDVALSSG SACTSASLEP SYVLRAIGTD
410 420 430 440 450
EDLAHSSIRF GIGRFTTEEE VDYTAEKCIH HVKRLREMSP LWEMVQDGID

LKSIKWTQH
Length:459
Mass (Da):50,570
Last modified:September 18, 2013 - v3
Checksum:i986D636C5506C7B0
GO
Isoform Cytoplasmic (identifier: Q9Z1J3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.

Note: No experimental confirmation available.
Show »
Length:397
Mass (Da):43,972
Checksum:iCB0F1E7025E4E050
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221G → R in CAA10916 (PubMed:9738949).Curated1
Sequence conflicti334N → K in CAA10916 (PubMed:9738949).Curated1
Sequence conflicti394L → F in CAA10916 (PubMed:9738949).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186471 – 62Missing in isoform Cytoplasmic. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222660 mRNA. Translation: CAA10916.1.
AK075575 mRNA. Translation: BAC35831.1.
AK132838 mRNA. Translation: BAE21386.1.
AK148527 mRNA. Translation: BAE28601.1.
AK159828 mRNA. Translation: BAE35407.1.
AL833786 Genomic DNA. No translation available.
BX649640 Genomic DNA. No translation available.
CH466551 Genomic DNA. Translation: EDL06179.1.
CCDSiCCDS16962.1. [Q9Z1J3-1]
RefSeqiNP_035041.2. NM_010911.2. [Q9Z1J3-1]
UniGeneiMm.283604.

Genome annotation databases

EnsembliENSMUST00000029147; ENSMUSP00000029147; ENSMUSG00000027618. [Q9Z1J3-1]
GeneIDi18041.
KEGGimmu:18041.
UCSCiuc008nmj.2. mouse. [Q9Z1J3-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222660 mRNA. Translation: CAA10916.1.
AK075575 mRNA. Translation: BAC35831.1.
AK132838 mRNA. Translation: BAE21386.1.
AK148527 mRNA. Translation: BAE28601.1.
AK159828 mRNA. Translation: BAE35407.1.
AL833786 Genomic DNA. No translation available.
BX649640 Genomic DNA. No translation available.
CH466551 Genomic DNA. Translation: EDL06179.1.
CCDSiCCDS16962.1. [Q9Z1J3-1]
RefSeqiNP_035041.2. NM_010911.2. [Q9Z1J3-1]
UniGeneiMm.283604.

3D structure databases

ProteinModelPortaliQ9Z1J3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z1J3. 1 interactor.
STRINGi10090.ENSMUSP00000029147.

PTM databases

iPTMnetiQ9Z1J3.
PhosphoSitePlusiQ9Z1J3.

2D gel databases

REPRODUCTION-2DPAGEQ9Z1J3.

Proteomic databases

PaxDbiQ9Z1J3.
PeptideAtlasiQ9Z1J3.
PRIDEiQ9Z1J3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029147; ENSMUSP00000029147; ENSMUSG00000027618. [Q9Z1J3-1]
GeneIDi18041.
KEGGimmu:18041.
UCSCiuc008nmj.2. mouse. [Q9Z1J3-1]

Organism-specific databases

CTDi9054.
MGIiMGI:1316706. Nfs1.

Phylogenomic databases

eggNOGiKOG1549. Eukaryota.
COG1104. LUCA.
GeneTreeiENSGT00530000063513.
HOGENOMiHOG000017510.
HOVERGENiHBG003708.
InParanoidiQ9Z1J3.
KOiK04487.
OMAiEPIQSGG.
OrthoDBiEOG091G072R.
PhylomeDBiQ9Z1J3.
TreeFamiTF105658.

Enzyme and pathway databases

BRENDAi2.8.1.7. 3474.
ReactomeiR-MMU-1362409. Mitochondrial iron-sulfur cluster biogenesis.
R-MMU-947581. Molybdenum cofactor biosynthesis.

Miscellaneous databases

ChiTaRSiNfs1. mouse.
PROiQ9Z1J3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027618.
CleanExiMM_NFS1.
ExpressionAtlasiQ9Z1J3. baseline and differential.
GenevisibleiQ9Z1J3. MM.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_00331. Cys_desulf_IscS. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR020578. Aminotrans_V_PyrdxlP_BS.
IPR010240. Cys_deSase_IscS.
IPR016454. Cysteine_dSase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF005572. NifS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR02006. IscS. 1 hit.
PROSITEiPS00595. AA_TRANSFER_CLASS_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNFS1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1J3
Secondary accession number(s): Q8C6I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: September 18, 2013
Last modified: November 30, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.