Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase Nek4

Gene

Nek4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for normal entry into proliferative arrest after a limited number of cell divisions, also called replicative senescence. Required for normal cell cycle arrest in response to double-stranded DNA damage (By similarity). Protein kinase that seems to act exclusively upon threonine residues.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mn2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35ATPPROSITE-ProRule annotation1
Active sitei131Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 20ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • manganese ion binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB
  • receptor signaling protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase Nek4 (EC:2.7.11.11 Publication)
Alternative name(s):
Never in mitosis A-related kinase 4
Short name:
NimA-related protein kinase 4
Serine/threonine-protein kinase 21 Publication
Gene namesi
Name:Nek4
Synonyms:Stk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1344404. Nek4.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell projectioncilium By similarity

GO - Cellular componenti

  • cilium Source: UniProtKB-SubCell
  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864261 – 792Serine/threonine-protein kinase Nek4Add BLAST792

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei165Phosphothreonine; by autocatalysisBy similarity1
Modified residuei340PhosphoserineBy similarity1
Modified residuei343PhosphoserineBy similarity1
Modified residuei566N6-methyllysineBy similarity1
Modified residuei675PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9Z1J2.
PRIDEiQ9Z1J2.

PTM databases

iPTMnetiQ9Z1J2.
PhosphoSitePlusiQ9Z1J2.

Expressioni

Tissue specificityi

Expressed ubiquitously among various organs and is up-regulated in the testis.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057915.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1J2.
SMRiQ9Z1J2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 261Protein kinasePROSITE-ProRule annotationAdd BLAST256

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0589. Eukaryota.
ENOG410Y7JF. LUCA.
HOGENOMiHOG000294177.
HOVERGENiHBG031740.
InParanoidiQ9Z1J2.
KOiK08857.
PhylomeDBiQ9Z1J2.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z1J2-1) [UniParc]FASTAAdd to basket
Also known as: mSTK2L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPQAAYCYMR VVGRGSYGEV TLVKHRRDGK QYVIKKLNLR NASSRERRAA
60 70 80 90 100
EQEAQLLSQL KHPNIVTYKE SWEGGDGLLY IVMGFCEGGD LYRKLKEQKG
110 120 130 140 150
QLLPESQVVE WFVQIAMALQ YLHEKHILHR DLKTQNVFLT RTNIIKVGDL
160 170 180 190 200
GIARVLENHG DMASTLIGTP YYMSPELFSN KPYNYKSDVW ALGCCVYEMA
210 220 230 240 250
TLKHAFNAKD MNSLVYRIIE GKLPPMPKVY STELAELIRT MLSRRPEERP
260 270 280 290 300
SVRSILRQPY IKHHISLFLE ATKAKTSKNN VKNCDSRAKP VAAVVSRKEE
310 320 330 340 350
SNTDVIHYQP RSSEGSALHV MGEDKCLSQE KPVDIGPLRS PASLEGHTGK
360 370 380 390 400
QDMNNTGESC ATISRINIDI LPAERRDSAN AGVVQESQPQ HVDAADEVDS
410 420 430 440 450
QCSISQEKER LQGNTKSSDQ PGNLLPRRSS DGGDGEGSEL VKPLYPSNKD
460 470 480 490 500
QKPDQDQVTG IIENQDSIHP RSQPHSSMSE PSLSRQRRQK KREQTAHSGT
510 520 530 540 550
KSQFQELPPR LLPSYPGIGK VDIIATQQND GNQGGPVAGC VNSSRTSSTA
560 570 580 590 600
SAKDRPLSAR ERRRLKQSQE EMLPSGPAVQ RTPSAVEPLK PQEEDQPIPA
610 620 630 640 650
QRFSSDCSIT QMNHTLPREK EKRLMHGLSE DELSSSTSST DKSDGDSREG
660 670 680 690 700
KSHTNEMKDL VQLMTQTLRL EAKESCEDLQ VLNPGSEFRL HRKYRDTLVL
710 720 730 740 750
HGKVAEEVEP HCTELPTGII PGSEKIRRIV EVLRADVIQG LGIQLLEQVF
760 770 780 790
DLLGEEDELE REARLQEHMG DKYTTYCVKA RQLKFFEENV SF
Length:792
Mass (Da):88,994
Last modified:May 1, 1999 - v1
Checksum:iCF9187311C807A1D
GO
Isoform 2 (identifier: Q9Z1J2-2) [UniParc]FASTAAdd to basket
Also known as: mSTK2S

The sequence of this isoform differs from the canonical sequence as follows:
     456-503: Missing.

Show »
Length:744
Mass (Da):83,542
Checksum:i82E1AAF3E9DCC72A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti499G → R in AAD16287 (PubMed:10393247).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007001456 – 503Missing in isoform 2. 1 PublicationAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223071 mRNA. Translation: CAA11072.1.
Y09234 mRNA. Translation: CAA70436.1.
AF099067 mRNA. Translation: AAD16287.1.
CCDSiCCDS26902.1. [Q9Z1J2-1]
PIRiJC7122.
RefSeqiNP_001295257.1. NM_001308328.1. [Q9Z1J2-2]
NP_035979.1. NM_011849.3.
UniGeneiMm.251494.

Genome annotation databases

GeneIDi23955.
KEGGimmu:23955.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223071 mRNA. Translation: CAA11072.1.
Y09234 mRNA. Translation: CAA70436.1.
AF099067 mRNA. Translation: AAD16287.1.
CCDSiCCDS26902.1. [Q9Z1J2-1]
PIRiJC7122.
RefSeqiNP_001295257.1. NM_001308328.1. [Q9Z1J2-2]
NP_035979.1. NM_011849.3.
UniGeneiMm.251494.

3D structure databases

ProteinModelPortaliQ9Z1J2.
SMRiQ9Z1J2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000057915.

PTM databases

iPTMnetiQ9Z1J2.
PhosphoSitePlusiQ9Z1J2.

Proteomic databases

PaxDbiQ9Z1J2.
PRIDEiQ9Z1J2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23955.
KEGGimmu:23955.

Organism-specific databases

CTDi6787.
MGIiMGI:1344404. Nek4.

Phylogenomic databases

eggNOGiKOG0589. Eukaryota.
ENOG410Y7JF. LUCA.
HOGENOMiHOG000294177.
HOVERGENiHBG031740.
InParanoidiQ9Z1J2.
KOiK08857.
PhylomeDBiQ9Z1J2.

Miscellaneous databases

PROiQ9Z1J2.
SOURCEiSearch...

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEK4_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1J2
Secondary accession number(s): O35673, Q9R1J1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.