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Protein

Transcription factor 7-like 1

Gene

Tcf7l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi342 – 41069HMG boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • beta-catenin binding Source: MGI
  • chromatin binding Source: MGI
  • DNA binding Source: MGI
  • sequence-specific DNA binding Source: GO_Central
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription regulatory region DNA binding Source: GO_Central

GO - Biological processi

  • anterior/posterior axis specification, embryo Source: MGI
  • axial mesoderm morphogenesis Source: MGI
  • canonical Wnt signaling pathway Source: GO_Central
  • negative regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter during mitosis Source: MGI
  • regulation of stem cell population maintenance Source: MGI
  • skin development Source: MGI
  • somatic stem cell population maintenance Source: MGI
  • stem cell differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation, Wnt signaling pathway

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 7-like 1
Alternative name(s):
HMG box transcription factor 3
Short name:
TCF-3
Short name:
mTCF-3
Gene namesi
Name:Tcf7l1
Synonyms:Tcf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1202876. Tcf7l1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
  • transcription factor complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi383 – 3831L → P: Abolishes DNA-binding and transactivator/repressor activity; when associated with I-407. 1 Publication
Mutagenesisi407 – 4071P → I: Abolishes DNA-binding and transactivator/repressor activity; when associated with P-383. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 584584Transcription factor 7-like 1PRO_0000048615Add
BLAST

Proteomic databases

MaxQBiQ9Z1J1.
PaxDbiQ9Z1J1.
PRIDEiQ9Z1J1.

PTM databases

iPTMnetiQ9Z1J1.
PhosphoSiteiQ9Z1J1.

Expressioni

Tissue specificityi

Detected in the basal layer of epidermis and in outer root sheath and bulge of hair follicles.1 Publication

Gene expression databases

CleanExiMM_TCF3.

Interactioni

Subunit structurei

Binds the armadillo repeat of CTNNB1 and forms a stable complex.

GO - Molecular functioni

  • beta-catenin binding Source: MGI

Protein-protein interaction databases

BioGridi204008. 3 interactions.
DIPiDIP-49605N.
IntActiQ9Z1J1. 4 interactions.
STRINGi10090.ENSMUSP00000109687.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1J1.
SMRiQ9Z1J1. Positions 29-67, 341-416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7171CTNNB1-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi417 – 4237Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 2622Gly-richAdd
BLAST
Compositional biasi167 – 322156Pro-richAdd
BLAST

Domaini

The putative Groucho interaction domain between the N-terminal CTNNB1 binding domain and the HMG-box is necessary for repression of the transactivation mediated by TCF7L1 and CTNNB1.

Sequence similaritiesi

Belongs to the TCF/LEF family.Curated
Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3248. Eukaryota.
ENOG41109RU. LUCA.
HOVERGENiHBG000419.
InParanoidiQ9Z1J1.
KOiK04490.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
4.10.900.10. 1 hit.
InterProiIPR027397. Catenin_binding_dom.
IPR013558. CTNNB1-bd_N.
IPR009071. HMG_box_dom.
IPR024940. TCF/LEF.
IPR028778. Tcf7l1.
[Graphical view]
PANTHERiPTHR10373. PTHR10373. 1 hit.
PTHR10373:SF25. PTHR10373:SF25. 1 hit.
PfamiPF08347. CTNNB1_binding. 1 hit.
PF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQLGGGRGG AGGGGGGSGA GATSGGDDLG ANDELIPFQD EGGEEQEPSS
60 70 80 90 100
DTASAQRDLD EVKSSLVNES ENQSSSSDSE AERRPQPARD AFQKPRDYFA
110 120 130 140 150
EVRRPQDGAF FKGGAYPGYP FLMIPDLSSP YLSNGPLSPG GARTYLQMKW
160 170 180 190 200
PLLDVPSSAT VKDTRSPSPA HLSNKVPVVQ HPHHMHPLTP LITYSNDHFS
210 220 230 240 250
PASPPTHLSP EIDPKTGIPR PPHPSELSPY YPLSPGAVGQ IPHPLGWLVP
260 270 280 290 300
QQGQPMYSLP PGGFRHPYPA LAMNASMSSL VSSRFPHMVA PAHPGLPTSG
310 320 330 340 350
IPHPAIVSPI VKQEPAAPSL SPAVSAKSPV TVKKEEEKKP HVKKPLNAFM
360 370 380 390 400
LYMKEMRAKV VAECTLKESA AINQILGRKW HNLSREEQAK YYELARKERQ
410 420 430 440 450
LHAQLYPTWS ARDNYGKKKK RKREKQLSQT QSQQQIQEAE GALASKSKKP
460 470 480 490 500
CIQYLPPEKP CDSPASSHGS ALDSPATPSA ALASPAAPAA THSEQAQPLS
510 520 530 540 550
LTTKPEARAQ LALHSAAFLS AKAAASNSSQ MGSQPPLLSR PLPLGSMPAA
560 570 580
LLTSPPTFPA TLHAHQALPV LQAQPLSLVT KSAH
Length:584
Mass (Da):62,298
Last modified:March 25, 2003 - v2
Checksum:iBA8B4F570AFC72CB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141G → P in CAA11201 (Ref. 2) Curated
Sequence conflicti301 – 3011I → M in CAA11201 (Ref. 2) Curated
Sequence conflicti404 – 4052QL → HV in CAA11201 (Ref. 2) Curated
Sequence conflicti463 – 4631S → I in CAA11201 (Ref. 2) Curated
Sequence conflicti471 – 4711A → T (Ref. 2) Curated
Sequence conflicti471 – 4711A → T (Ref. 3) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223069 mRNA. Translation: CAA11070.1.
AJ223233 mRNA. Translation: CAA11201.1.
AF057691 mRNA. Translation: AAC13696.1.
RefSeqiNP_033358.2. NM_009332.3.
UniGeneiMm.440067.

Genome annotation databases

GeneIDi21415.
KEGGimmu:21415.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223069 mRNA. Translation: CAA11070.1.
AJ223233 mRNA. Translation: CAA11201.1.
AF057691 mRNA. Translation: AAC13696.1.
RefSeqiNP_033358.2. NM_009332.3.
UniGeneiMm.440067.

3D structure databases

ProteinModelPortaliQ9Z1J1.
SMRiQ9Z1J1. Positions 29-67, 341-416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204008. 3 interactions.
DIPiDIP-49605N.
IntActiQ9Z1J1. 4 interactions.
STRINGi10090.ENSMUSP00000109687.

PTM databases

iPTMnetiQ9Z1J1.
PhosphoSiteiQ9Z1J1.

Proteomic databases

MaxQBiQ9Z1J1.
PaxDbiQ9Z1J1.
PRIDEiQ9Z1J1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi21415.
KEGGimmu:21415.

Organism-specific databases

CTDi83439.
MGIiMGI:1202876. Tcf7l1.

Phylogenomic databases

eggNOGiKOG3248. Eukaryota.
ENOG41109RU. LUCA.
HOVERGENiHBG000419.
InParanoidiQ9Z1J1.
KOiK04490.

Miscellaneous databases

ChiTaRSiTcf7l1. mouse.
PROiQ9Z1J1.
SOURCEiSearch...

Gene expression databases

CleanExiMM_TCF3.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
4.10.900.10. 1 hit.
InterProiIPR027397. Catenin_binding_dom.
IPR013558. CTNNB1-bd_N.
IPR009071. HMG_box_dom.
IPR024940. TCF/LEF.
IPR028778. Tcf7l1.
[Graphical view]
PANTHERiPTHR10373. PTHR10373. 1 hit.
PTHR10373:SF25. PTHR10373:SF25. 1 hit.
PfamiPF08347. CTNNB1_binding. 1 hit.
PF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTF7L1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1J1
Secondary accession number(s): O70450, O70573
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 25, 2003
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.