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Protein

Transcription factor 7-like 1

Gene

Tcf7l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi342 – 410HMG boxPROSITE-ProRule annotationAdd BLAST69

GO - Molecular functioni

  • beta-catenin binding Source: MGI
  • chromatin binding Source: MGI
  • DNA binding Source: MGI
  • DNA binding transcription factor activity Source: MGI
  • sequence-specific DNA binding Source: GO_Central
  • transcription regulatory region DNA binding Source: GO_Central

GO - Biological processi

  • anterior/posterior axis specification, embryo Source: MGI
  • axial mesoderm morphogenesis Source: MGI
  • canonical Wnt signaling pathway Source: GO_Central
  • negative regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • regulation of stem cell population maintenance Source: MGI
  • skin development Source: MGI
  • somatic stem cell population maintenance Source: MGI
  • stem cell differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 7-like 1
Alternative name(s):
HMG box transcription factor 3
Short name:
TCF-3
Short name:
mTCF-3
Gene namesi
Name:Tcf7l1
Synonyms:Tcf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1202876 Tcf7l1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi383L → P: Abolishes DNA-binding and transactivator/repressor activity; when associated with I-407. 1 Publication1
Mutagenesisi407P → I: Abolishes DNA-binding and transactivator/repressor activity; when associated with P-383. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000486151 – 584Transcription factor 7-like 1Add BLAST584

Proteomic databases

MaxQBiQ9Z1J1
PaxDbiQ9Z1J1
PRIDEiQ9Z1J1

PTM databases

iPTMnetiQ9Z1J1
PhosphoSitePlusiQ9Z1J1

Expressioni

Tissue specificityi

Detected in the basal layer of epidermis and in outer root sheath and bulge of hair follicles.1 Publication

Gene expression databases

CleanExiMM_TCF3

Interactioni

Subunit structurei

Binds the armadillo repeat of CTNNB1 and forms a stable complex.

GO - Molecular functioni

  • beta-catenin binding Source: MGI

Protein-protein interaction databases

BioGridi204008, 3 interactors
DIPiDIP-49605N
IntActiQ9Z1J1, 5 interactors
STRINGi10090.ENSMUSP00000109687

Structurei

3D structure databases

ProteinModelPortaliQ9Z1J1
SMRiQ9Z1J1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 71CTNNB1-bindingBy similarityAdd BLAST71

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi417 – 423Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 26Gly-richAdd BLAST22
Compositional biasi167 – 322Pro-richAdd BLAST156

Domaini

The putative Groucho interaction domain between the N-terminal CTNNB1 binding domain and the HMG-box is necessary for repression of the transactivation mediated by TCF7L1 and CTNNB1.

Sequence similaritiesi

Belongs to the TCF/LEF family.Curated

Phylogenomic databases

eggNOGiKOG3248 Eukaryota
ENOG41109RU LUCA
HOVERGENiHBG000419
InParanoidiQ9Z1J1
KOiK04490

Family and domain databases

Gene3Di1.10.30.10, 1 hit
4.10.900.10, 1 hit
InterProiView protein in InterPro
IPR027397 Catenin_binding_dom_sf
IPR013558 CTNNB1-bd_N
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
IPR024940 TCF/LEF
IPR028773 TCF7L
PANTHERiPTHR10373 PTHR10373, 1 hit
PTHR10373:SF25 PTHR10373:SF25, 1 hit
PfamiView protein in Pfam
PF08347 CTNNB1_binding, 1 hit
PF00505 HMG_box, 1 hit
SMARTiView protein in SMART
SM00398 HMG, 1 hit
SUPFAMiSSF47095 SSF47095, 1 hit
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z1J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQLGGGRGG AGGGGGGSGA GATSGGDDLG ANDELIPFQD EGGEEQEPSS
60 70 80 90 100
DTASAQRDLD EVKSSLVNES ENQSSSSDSE AERRPQPARD AFQKPRDYFA
110 120 130 140 150
EVRRPQDGAF FKGGAYPGYP FLMIPDLSSP YLSNGPLSPG GARTYLQMKW
160 170 180 190 200
PLLDVPSSAT VKDTRSPSPA HLSNKVPVVQ HPHHMHPLTP LITYSNDHFS
210 220 230 240 250
PASPPTHLSP EIDPKTGIPR PPHPSELSPY YPLSPGAVGQ IPHPLGWLVP
260 270 280 290 300
QQGQPMYSLP PGGFRHPYPA LAMNASMSSL VSSRFPHMVA PAHPGLPTSG
310 320 330 340 350
IPHPAIVSPI VKQEPAAPSL SPAVSAKSPV TVKKEEEKKP HVKKPLNAFM
360 370 380 390 400
LYMKEMRAKV VAECTLKESA AINQILGRKW HNLSREEQAK YYELARKERQ
410 420 430 440 450
LHAQLYPTWS ARDNYGKKKK RKREKQLSQT QSQQQIQEAE GALASKSKKP
460 470 480 490 500
CIQYLPPEKP CDSPASSHGS ALDSPATPSA ALASPAAPAA THSEQAQPLS
510 520 530 540 550
LTTKPEARAQ LALHSAAFLS AKAAASNSSQ MGSQPPLLSR PLPLGSMPAA
560 570 580
LLTSPPTFPA TLHAHQALPV LQAQPLSLVT KSAH
Length:584
Mass (Da):62,298
Last modified:March 25, 2003 - v2
Checksum:iBA8B4F570AFC72CB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114G → P in CAA11201 (Ref. 2) Curated1
Sequence conflicti301I → M in CAA11201 (Ref. 2) Curated1
Sequence conflicti404 – 405QL → HV in CAA11201 (Ref. 2) Curated2
Sequence conflicti463S → I in CAA11201 (Ref. 2) Curated1
Sequence conflicti471A → T (Ref. 2) Curated1
Sequence conflicti471A → T (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223069 mRNA Translation: CAA11070.1
AJ223233 mRNA Translation: CAA11201.1
AF057691 mRNA Translation: AAC13696.1
RefSeqiNP_033358.2, NM_009332.3
UniGeneiMm.440067

Genome annotation databases

GeneIDi21415
KEGGimmu:21415

Similar proteinsi

Entry informationi

Entry nameiTF7L1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1J1
Secondary accession number(s): O70450, O70573
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: March 25, 2003
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health