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Protein

V-type proton ATPase 116 kDa subunit a isoform 1

Gene

Atp6v0a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-77387. Insulin receptor recycling.
R-MMU-917977. Transferrin endocytosis and recycling.
R-MMU-983712. Ion channel transport.

Protein family/group databases

TCDBi3.A.2.2.6. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase 116 kDa subunit a isoform 1
Short name:
V-ATPase 116 kDa isoform a1
Alternative name(s):
Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit
Vacuolar adenosine triphosphatase subunit Ac116
Vacuolar proton pump subunit 1
Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1
Gene namesi
Name:Atp6v0a1
Synonyms:Atp6n1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:103286. Atp6v0a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 395395CytoplasmicSequence analysisAdd
BLAST
Transmembranei396 – 41419HelicalSequence analysisAdd
BLAST
Topological domaini415 – 4162VacuolarSequence analysis
Transmembranei417 – 43317HelicalSequence analysisAdd
BLAST
Topological domaini434 – 44815CytoplasmicSequence analysisAdd
BLAST
Transmembranei449 – 47830HelicalSequence analysisAdd
BLAST
Topological domaini479 – 54264VacuolarSequence analysisAdd
BLAST
Transmembranei543 – 56220HelicalSequence analysisAdd
BLAST
Topological domaini563 – 58018CytoplasmicSequence analysisAdd
BLAST
Transmembranei581 – 60121HelicalSequence analysisAdd
BLAST
Topological domaini602 – 64645VacuolarSequence analysisAdd
BLAST
Transmembranei647 – 66620HelicalSequence analysisAdd
BLAST
Topological domaini667 – 72660CytoplasmicSequence analysisAdd
BLAST
Transmembranei727 – 75125HelicalSequence analysisAdd
BLAST
Topological domaini752 – 77221VacuolarSequence analysisAdd
BLAST
Transmembranei773 – 81139HelicalSequence analysisAdd
BLAST
Topological domaini812 – 83928CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 839839V-type proton ATPase 116 kDa subunit a isoform 1PRO_0000119212Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei257 – 2571PhosphothreonineCombined sources
Modified residuei367 – 3671PhosphothreonineBy similarity
Modified residuei371 – 3711PhosphotyrosineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z1G4.
PaxDbiQ9Z1G4.
PeptideAtlasiQ9Z1G4.
PRIDEiQ9Z1G4.

PTM databases

iPTMnetiQ9Z1G4.
PhosphoSiteiQ9Z1G4.
SwissPalmiQ9Z1G4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000019302.
CleanExiMM_ATP6V0A1.
ExpressionAtlasiQ9Z1G4. baseline and differential.
GenevisibleiQ9Z1G4. MM.

Interactioni

Subunit structurei

The V-ATPase is a heteromultimeric enzyme composed of at least thirteen different subunits. It has a membrane peripheral V1 sector for ATP hydrolysis and an integral V0 for proton translocation. The V1 sector comprises subunits A-H, whereas V0 includes subunits a, d, c, c', and c''.

Binary interactionsi

WithEntry#Exp.IntActNotes
Psen1P497692EBI-771149,EBI-990067

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198267. 5 interactions.
IntActiQ9Z1G4. 6 interactions.
MINTiMINT-1866025.
STRINGi10090.ENSMUSP00000099399.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1G4.
SMRiQ9Z1G4. Positions 10-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the V-ATPase 116 kDa subunit family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2189. Eukaryota.
COG1269. LUCA.
GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
HOVERGENiHBG014606.
InParanoidiQ9Z1G4.
KOiK02154.
OMAiFTLYNWT.
OrthoDBiEOG091G01BI.
TreeFamiTF300346.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A1-II (identifier: Q9Z1G4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGELFRSEEM TLAQLFLQSE AAYCCVSELG ELGKVQFRDL NPDVNVFQRK
60 70 80 90 100
FVNEVRRCEE MDRKLRFVEK EIRKANIPIM DTGENPEVPF PRDMIDLEAN
110 120 130 140 150
FEKIENELKE INTNQEALKR NFLELTELKF ILRKTQQFFD EAELHHQQMA
160 170 180 190 200
DPDLLEESSS LLEPNEMGRG APLRLGFVAG VINRERIPTF ERMLWRVCRG
210 220 230 240 250
NVFLRQAEIE NPLEDPVTGD YVHKSVFIIF FQGDQLKNRV KKICEGFRAS
260 270 280 290 300
LYPCPETPQE RKEMASGVNT RIDDLQMVLN QTEDHRQRVL QAAAKNIRVW
310 320 330 340 350
FIKVRKMKAI YHTLNLCNID VTQKCLIAEV WCPVTDLDSI QFALRRGTEH
360 370 380 390 400
SGSTVPSILN RMQTNQTPPT YNKTNKFTHG FQNIVDAYGI GTYREINPAP
410 420 430 440 450
YTVITFPFLF AVMFGDFGHG ILMTLFAVWM VLRESRILSQ KHENEMFSMV
460 470 480 490 500
FSGRYIILLM GLFSIYTGLI YNDCFSKSLN IFGSSWSVRP MFTQGNWTEE
510 520 530 540 550
TLLGSSVLQL NPAIPGVFGG PYPFGIDPIW NIATNKLTFL NSFKMKMSVI
560 570 580 590 600
LGIIHMLFGV SLSLFNHIYF KKPLNIYFGF IPEIIFMSSL FGYLVILIFY
610 620 630 640 650
KWTAYDAHSS RNAPSLLIHF INMFLFSYPE SGNAMLYSGQ KGIQCFLIVV
660 670 680 690 700
AMLCVPWMLL FKPLILRHQY LRKKHLGTLN FGGIRVGNGP TEEDAEIIQH
710 720 730 740 750
DQLSTHSEDA EEFDFGDTMV HQAIHTIEYC LGCISNTASY LRLWALSLAH
760 770 780 790 800
AQLSEVLWTM VIHIGLHVRS LAGGLGLFFI FAAFATLTVA ILLIMEGLSA
810 820 830
FLHALRLHWV EFQNKFYTGT GFKFLPFSFE HIREGKFDE
Length:839
Mass (Da):96,467
Last modified:July 27, 2011 - v3
Checksum:iE102667721450C06
GO
Isoform A1-I (identifier: Q9Z1G4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     142-148: Missing.
     712-712: E → EPTEDEV

Show »
Length:838
Mass (Da):96,294
Checksum:i1A96E0FA5D521FE0
GO
Isoform A1-III (identifier: Q9Z1G4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     142-148: Missing.

Show »
Length:832
Mass (Da):95,623
Checksum:i62BD9D631F7484AF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361Q → N in AAC83083 (Ref. 1) Curated
Sequence conflicti88 – 925VPFPR → APLPW in AAC83083 (Ref. 1) Curated
Sequence conflicti112 – 1121N → D in AAC83083 (Ref. 1) Curated
Sequence conflicti190 – 1901F → S in AAC83083 (Ref. 1) Curated
Sequence conflicti262 – 2621K → T in AAC83083 (Ref. 1) Curated
Sequence conflicti337 – 3371L → P in AAC83083 (Ref. 1) Curated
Sequence conflicti357 – 3571S → F in AAC83083 (Ref. 1) Curated
Sequence conflicti415 – 4151G → R in AAC83083 (Ref. 1) Curated
Sequence conflicti518 – 5181F → L in AAC83083 (Ref. 1) Curated
Sequence conflicti683 – 6831G → W in AAC83083 (Ref. 1) Curated
Sequence conflicti803 – 8031H → L in AAC83083 (Ref. 1) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti194 – 1941L → F.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei142 – 1487Missing in isoform A1-I and isoform A1-III. 1 PublicationVSP_000342
Alternative sequencei712 – 7121E → EPTEDEV in isoform A1-I. CuratedVSP_000343

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13836 mRNA. Translation: AAC83083.1.
AF218249 mRNA. Translation: AAF59918.1.
AF218250 mRNA. Translation: AAF59919.1.
AF218251 mRNA. Translation: AAF59920.1.
AB022321 mRNA. Translation: BAA93005.1.
AL591425 Genomic DNA. Translation: CAM19437.1.
CH466677 Genomic DNA. Translation: EDL03866.1.
CCDSiCCDS25443.1. [Q9Z1G4-2]
CCDS56808.1. [Q9Z1G4-1]
CCDS56809.1. [Q9Z1G4-3]
RefSeqiNP_001229979.1. NM_001243050.1. [Q9Z1G4-3]
NP_001229980.1. NM_001243051.1. [Q9Z1G4-1]
NP_058616.1. NM_016920.3. [Q9Z1G4-2]
UniGeneiMm.340818.
Mm.475829.

Genome annotation databases

EnsembliENSMUST00000044721; ENSMUSP00000044838; ENSMUSG00000019302. [Q9Z1G4-2]
ENSMUST00000092663; ENSMUSP00000090333; ENSMUSG00000019302. [Q9Z1G4-3]
ENSMUST00000103110; ENSMUSP00000099399; ENSMUSG00000019302. [Q9Z1G4-1]
GeneIDi11975.
KEGGimmu:11975.
UCSCiuc007lmt.2. mouse. [Q9Z1G4-3]
uc007lmu.2. mouse. [Q9Z1G4-2]
uc007lmx.2. mouse. [Q9Z1G4-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13836 mRNA. Translation: AAC83083.1.
AF218249 mRNA. Translation: AAF59918.1.
AF218250 mRNA. Translation: AAF59919.1.
AF218251 mRNA. Translation: AAF59920.1.
AB022321 mRNA. Translation: BAA93005.1.
AL591425 Genomic DNA. Translation: CAM19437.1.
CH466677 Genomic DNA. Translation: EDL03866.1.
CCDSiCCDS25443.1. [Q9Z1G4-2]
CCDS56808.1. [Q9Z1G4-1]
CCDS56809.1. [Q9Z1G4-3]
RefSeqiNP_001229979.1. NM_001243050.1. [Q9Z1G4-3]
NP_001229980.1. NM_001243051.1. [Q9Z1G4-1]
NP_058616.1. NM_016920.3. [Q9Z1G4-2]
UniGeneiMm.340818.
Mm.475829.

3D structure databases

ProteinModelPortaliQ9Z1G4.
SMRiQ9Z1G4. Positions 10-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198267. 5 interactions.
IntActiQ9Z1G4. 6 interactions.
MINTiMINT-1866025.
STRINGi10090.ENSMUSP00000099399.

Protein family/group databases

TCDBi3.A.2.2.6. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

PTM databases

iPTMnetiQ9Z1G4.
PhosphoSiteiQ9Z1G4.
SwissPalmiQ9Z1G4.

Proteomic databases

MaxQBiQ9Z1G4.
PaxDbiQ9Z1G4.
PeptideAtlasiQ9Z1G4.
PRIDEiQ9Z1G4.

Protocols and materials databases

DNASUi11975.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044721; ENSMUSP00000044838; ENSMUSG00000019302. [Q9Z1G4-2]
ENSMUST00000092663; ENSMUSP00000090333; ENSMUSG00000019302. [Q9Z1G4-3]
ENSMUST00000103110; ENSMUSP00000099399; ENSMUSG00000019302. [Q9Z1G4-1]
GeneIDi11975.
KEGGimmu:11975.
UCSCiuc007lmt.2. mouse. [Q9Z1G4-3]
uc007lmu.2. mouse. [Q9Z1G4-2]
uc007lmx.2. mouse. [Q9Z1G4-1]

Organism-specific databases

CTDi535.
MGIiMGI:103286. Atp6v0a1.

Phylogenomic databases

eggNOGiKOG2189. Eukaryota.
COG1269. LUCA.
GeneTreeiENSGT00390000004941.
HOGENOMiHOG000037059.
HOVERGENiHBG014606.
InParanoidiQ9Z1G4.
KOiK02154.
OMAiFTLYNWT.
OrthoDBiEOG091G01BI.
TreeFamiTF300346.

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-77387. Insulin receptor recycling.
R-MMU-917977. Transferrin endocytosis and recycling.
R-MMU-983712. Ion channel transport.

Miscellaneous databases

ChiTaRSiAtp6v0a1. mouse.
PROiQ9Z1G4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019302.
CleanExiMM_ATP6V0A1.
ExpressionAtlasiQ9Z1G4. baseline and differential.
GenevisibleiQ9Z1G4. MM.

Family and domain databases

InterProiIPR002490. V-ATPase_116kDa_su.
IPR026028. V-type_ATPase_116kDa_su_euka.
[Graphical view]
PANTHERiPTHR11629. PTHR11629. 1 hit.
PfamiPF01496. V_ATPase_I. 1 hit.
[Graphical view]
PIRSFiPIRSF001293. ATP6V0A1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVPP1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1G4
Secondary accession number(s): A2A5A1
, Q9JHJ4, Q9JL13, Q9JL14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.