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Protein

Glycogen [starch] synthase, muscle

Gene

Gys1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.By similarity

Catalytic activityi

UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

Enzyme regulationi

Allosteric activation by glucose-6-phosphate. Phosphorylation reduces the activity towards UDP-glucose. When in the non-phosphorylated state, glycogen synthase does not require glucose-6-phosphate as an allosteric activator; when phosphorylated it does (By similarity).By similarity

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39UDP-glucoseBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glycogen biosynthetic process Source: MGI
  • heart development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-3322077. Glycogen synthesis.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiGT3. Glycosyltransferase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen [starch] synthase, muscle (EC:2.4.1.11)
Gene namesi
Name:Gys1
Synonyms:Gys, Gys3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:101805. Gys1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • inclusion body Source: MGI
  • membrane Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001947652 – 738Glycogen [starch] synthase, muscleAdd BLAST737

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8Phosphoserine; by AMPK and PKA1 Publication1
Modified residuei11PhosphoserineBy similarity1
Modified residuei412PhosphoserineCombined sources1
Modified residuei641Phosphoserine; by DYRK2, GSK3-alpha, GSK3-beta and PASKBy similarity1
Modified residuei645PhosphoserineCombined sources1
Modified residuei649PhosphoserineCombined sources1
Modified residuei652PhosphoserineBy similarity1
Modified residuei653PhosphoserineCombined sources1
Modified residuei657PhosphoserineCombined sources1
Modified residuei672PhosphoserineCombined sources1
Modified residuei698PhosphoserineBy similarity1
Modified residuei709PhosphoserineCombined sources1
Modified residuei711PhosphoserineBy similarity1
Modified residuei722PhosphothreonineCombined sources1
Modified residuei724PhosphothreonineCombined sources1
Modified residuei728PhosphoserineCombined sources1

Post-translational modificationi

Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Phosphorylated at Ser-641 by DYRK2, leading to inactivation. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme (By similarity). Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z1E4.
MaxQBiQ9Z1E4.
PaxDbiQ9Z1E4.
PeptideAtlasiQ9Z1E4.
PRIDEiQ9Z1E4.

PTM databases

iPTMnetiQ9Z1E4.
PhosphoSitePlusiQ9Z1E4.
SwissPalmiQ9Z1E4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003865.
GenevisibleiQ9Z1E4. MM.

Interactioni

Subunit structurei

Interacts with GYG1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200132. 1 interactor.
IntActiQ9Z1E4. 4 interactors.
MINTiMINT-4097092.
STRINGi10090.ENSMUSP00000003964.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1E4.
SMRiQ9Z1E4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 3 family.Curated

Phylogenomic databases

eggNOGiKOG3742. Eukaryota.
COG0438. LUCA.
GeneTreeiENSGT00390000018612.
HOGENOMiHOG000160890.
HOVERGENiHBG001960.
InParanoidiQ9Z1E4.
KOiK00693.
OMAiFYGHMDF.
OrthoDBiEOG091G0304.
PhylomeDBiQ9Z1E4.
TreeFamiTF300306.

Family and domain databases

InterProiIPR008631. Glycogen_synth.
[Graphical view]
PANTHERiPTHR10176. PTHR10176. 1 hit.
PfamiPF05693. Glycogen_syn. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1E4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLSRSLSVS SLPGLEDWED EFDPENAVLF EVAWEVANKV GGIYTVLQTK
60 70 80 90 100
AKVTGDEWGD NYYLVGPYTE QGVRTQVELL EPPTPELKRT LDSMNSKGCK
110 120 130 140 150
VYFGRWLIEG GPLVVLLDVG ASAWALERWK GELWDTCNIG VPWYDREAND
160 170 180 190 200
AVLFGFLTTW FLGEFLAQNE EKPYVVAHFH EWLAGVGLCL CRARRLPVAT
210 220 230 240 250
IFTTHATLLG RYLCAGAVDF YNNLENFNVD KEAGERQIYH RYCMERAAAH
260 270 280 290 300
CAHVFTTVSQ ITAIEAQHLL KRKPDIVTPN GLNVKKFSAM HEFQNLHAQS
310 320 330 340 350
KARIQEFVRG HFYGHLDFNL DKTLYFFIAG RYEFSNKGAD VFLEALARLN
360 370 380 390 400
YLLRVNGSEQ TVVAFFIMPA RTNNFNVETL KGQAVRKQLW DTANTVKEKF
410 420 430 440 450
GRKLYESLLV GSLPDMNKML DKEDFTMMKR AIFATQRQSF PPVCTHNMLD
460 470 480 490 500
DSSDPILTTI RRIGLFNSSA DRVKVIFHPE FLSSTSPLLP VDYEEFVRGC
510 520 530 540 550
HLGVFPSYYE PWGYTPAECT VMGIPSISTN LSGFGCFMEE HIADPSAYGI
560 570 580 590 600
YILDRRFRSL DDSCSQLTSF LYSFCQQSRR QRIIQRNRTE RLSDLLDWKY
610 620 630 640 650
LGRYYMSARH MALAKAFPDH FTYEPHEVDA TQGYRYPRPA SVPPSPSLSR
660 670 680 690 700
HSSPHQSEDE EEPRDGPLGE DSERYDEEEE AAKDRRNIRA PEWPRRASCS
710 720 730
SSTGGSKRSN SVDTGPSSSL STPTEPLSPT SSLGEERN
Length:738
Mass (Da):83,927
Last modified:December 16, 2008 - v2
Checksum:iD1F9252CA908FF69
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3L → R in CAA64322 (PubMed:8793107).Curated1
Sequence conflicti70E → V in AAD09457 (Ref. 2) Curated1
Sequence conflicti80L → M in BAE42275 (PubMed:16141072).Curated1
Sequence conflicti111 – 112GP → D in CAA64322 (PubMed:8793107).Curated2
Sequence conflicti120G → A in AAD09457 (Ref. 2) Curated1
Sequence conflicti148A → G in AAD09457 (Ref. 2) Curated1
Sequence conflicti154 – 155FG → YS in AAD09457 (Ref. 2) Curated2
Sequence conflicti191C → S in CAA64322 (PubMed:8793107).Curated1
Sequence conflicti208L → V in AAD09457 (Ref. 2) Curated1
Sequence conflicti228N → I in AAD09457 (Ref. 2) Curated1
Sequence conflicti581 – 582QR → HG in CAA64322 (PubMed:8793107).Curated2
Sequence conflicti640A → V in AAD09457 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94616 mRNA. Translation: CAA64322.1.
U53218 mRNA. Translation: AAD09457.1.
AK050813 mRNA. Translation: BAC34420.1.
AK171148 mRNA. Translation: BAE42275.1.
CH466603 Genomic DNA. Translation: EDL22855.1.
BC019389 mRNA. Translation: AAH19389.1.
BC131687 mRNA. Translation: AAI31688.1.
BC131688 mRNA. Translation: AAI31689.1.
BC152550 mRNA. Translation: AAI52551.1.
CCDSiCCDS21244.1.
RefSeqiNP_109603.2. NM_030678.3.
UniGeneiMm.275654.

Genome annotation databases

EnsembliENSMUST00000003964; ENSMUSP00000003964; ENSMUSG00000003865.
GeneIDi14936.
KEGGimmu:14936.
UCSCiuc009gve.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94616 mRNA. Translation: CAA64322.1.
U53218 mRNA. Translation: AAD09457.1.
AK050813 mRNA. Translation: BAC34420.1.
AK171148 mRNA. Translation: BAE42275.1.
CH466603 Genomic DNA. Translation: EDL22855.1.
BC019389 mRNA. Translation: AAH19389.1.
BC131687 mRNA. Translation: AAI31688.1.
BC131688 mRNA. Translation: AAI31689.1.
BC152550 mRNA. Translation: AAI52551.1.
CCDSiCCDS21244.1.
RefSeqiNP_109603.2. NM_030678.3.
UniGeneiMm.275654.

3D structure databases

ProteinModelPortaliQ9Z1E4.
SMRiQ9Z1E4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200132. 1 interactor.
IntActiQ9Z1E4. 4 interactors.
MINTiMINT-4097092.
STRINGi10090.ENSMUSP00000003964.

Protein family/group databases

CAZyiGT3. Glycosyltransferase Family 3.

PTM databases

iPTMnetiQ9Z1E4.
PhosphoSitePlusiQ9Z1E4.
SwissPalmiQ9Z1E4.

Proteomic databases

EPDiQ9Z1E4.
MaxQBiQ9Z1E4.
PaxDbiQ9Z1E4.
PeptideAtlasiQ9Z1E4.
PRIDEiQ9Z1E4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003964; ENSMUSP00000003964; ENSMUSG00000003865.
GeneIDi14936.
KEGGimmu:14936.
UCSCiuc009gve.1. mouse.

Organism-specific databases

CTDi2997.
MGIiMGI:101805. Gys1.

Phylogenomic databases

eggNOGiKOG3742. Eukaryota.
COG0438. LUCA.
GeneTreeiENSGT00390000018612.
HOGENOMiHOG000160890.
HOVERGENiHBG001960.
InParanoidiQ9Z1E4.
KOiK00693.
OMAiFYGHMDF.
OrthoDBiEOG091G0304.
PhylomeDBiQ9Z1E4.
TreeFamiTF300306.

Enzyme and pathway databases

UniPathwayiUPA00164.
ReactomeiR-MMU-3322077. Glycogen synthesis.

Miscellaneous databases

PROiQ9Z1E4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003865.
GenevisibleiQ9Z1E4. MM.

Family and domain databases

InterProiIPR008631. Glycogen_synth.
[Graphical view]
PANTHERiPTHR10176. PTHR10176. 1 hit.
PfamiPF05693. Glycogen_syn. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGYS1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1E4
Secondary accession number(s): P54859
, Q3TBN4, Q8BQG4, Q8VEB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: December 16, 2008
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.