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Protein

Eukaryotic translation initiation factor 3 subunit G

Gene

Eif3g

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit can bind 18S rRNA.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72689. Formation of a pool of free 40S subunits.
R-MMU-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit GUniRule annotation
Short name:
eIF3gUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 RNA-binding subunitUniRule annotation
Short name:
eIF-3 RNA-binding subunitUniRule annotation
Eukaryotic translation initiation factor 3 subunit 4UniRule annotation
eIF-3-deltaUniRule annotation
eIF3 p42UniRule annotation
eIF3 p44UniRule annotation
Gene namesi
Name:Eif3g
Synonyms:Eif3p42, Eif3s4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1858258. Eif3g.

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Nucleus UniRule annotation
  • Cytoplasmperinuclear region UniRule annotation

  • Note: Colocalizes with AIFM1 in the nucleus and perinuclear region.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001235112 – 320Eukaryotic translation initiation factor 3 subunit GAdd BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineBy similarity1
Modified residuei11PhosphoserineBy similarity1
Modified residuei38PhosphothreonineUniRule annotationBy similarity1
Modified residuei41PhosphothreonineCombined sources1
Modified residuei42PhosphoserineCombined sources1
Modified residuei189PhosphoserineBy similarity1
Modified residuei223PhosphoserineBy similarity1
Modified residuei264PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z1D1.
MaxQBiQ9Z1D1.
PaxDbiQ9Z1D1.
PRIDEiQ9Z1D1.

2D gel databases

REPRODUCTION-2DPAGEQ9Z1D1.

PTM databases

iPTMnetiQ9Z1D1.
PhosphoSitePlusiQ9Z1D1.
SwissPalmiQ9Z1D1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000070319.
ExpressionAtlasiQ9Z1D1. baseline and differential.
GenevisibleiQ9Z1D1. MM.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts (via C-terminus) with AIFM1 (via N-terminus) (By similarity).UniRule annotation

Protein-protein interaction databases

BioGridi207295. 5 interactors.
IntActiQ9Z1D1. 6 interactors.
MINTiMINT-1572167.
STRINGi10090.ENSMUSP00000004206.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1D1.
SMRiQ9Z1D1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini239 – 317RRMUniRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the eIF-3 subunit G family.UniRule annotation
Contains 1 RRM (RNA recognition motif) domain.UniRule annotation

Phylogenomic databases

eggNOGiKOG0122. Eukaryota.
ENOG410Y3CW. LUCA.
GeneTreeiENSGT00510000047802.
HOGENOMiHOG000239560.
HOVERGENiHBG026850.
InParanoidiQ9Z1D1.
KOiK03248.
OMAiIVRTYKI.
OrthoDBiEOG091G0GSV.
PhylomeDBiQ9Z1D1.
TreeFamiTF101516.

Family and domain databases

CDDicd12933. eIF3G. 1 hit.
Gene3Di3.30.70.330. 1 hit.
HAMAPiMF_03006. eIF3g. 1 hit.
InterProiIPR017334. eIF3_g.
IPR024675. eIF3g_N.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR10352. PTHR10352. 2 hits.
PfamiPF12353. eIF3g. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037949. Transl_init_eIF-3_RNA-bind. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1D1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTGDFDSKP SWADQVEEEG EDDKCVTSEL LKGIPLPTGD TSPEPELLPG
60 70 80 90 100
DPLPPPKEVI NGNIKTVTEY KIEEDGKKFK IVRTFRIETR KASKAVARRK
110 120 130 140 150
NWKKFGNSEF DPPGPNVATT TVSDDVSMTF ITSKEDLNCQ EEEDPMNKLK
160 170 180 190 200
GQKIVSCRIC KGDHWTTRCP YKDTLGPMQK ELAEQLGLST GEKEKLPGEL
210 220 230 240 250
EPVQAAQSKT GKYVPPSLRD GASRRGESMQ PNRRADDNAT IRVTNLSEDT
260 270 280 290 300
RETDLQELFR PFGSISRIYL AKDKTTGQSK GFAFISFHRR EDAARAIAGV
310 320
SGFGYDHLIL NVEWAKPSTN
Length:320
Mass (Da):35,638
Last modified:September 19, 2002 - v2
Checksum:i1B46670351E489AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17 – 18EE → AA in AAD00176 (Ref. 1) Curated2
Sequence conflicti21E → Q in AAD00176 (Ref. 1) Curated1
Sequence conflicti24K → T in AAD00176 (Ref. 1) Curated1
Sequence conflicti32K → T in AAD00176 (Ref. 1) Curated1
Sequence conflicti60I → P in AAD00176 (Ref. 1) Curated1
Sequence conflicti88E → S in AAD00176 (Ref. 1) Curated1
Sequence conflicti222 – 223AS → PA in AAD00176 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70733 mRNA. Translation: AAD00176.1.
AF108214 mRNA. Translation: AAF14221.1.
BC008511 mRNA. Translation: AAH08511.1.
CCDSiCCDS40547.1.
RefSeqiNP_058572.2. NM_016876.3.
UniGeneiMm.427309.

Genome annotation databases

EnsembliENSMUST00000004206; ENSMUSP00000004206; ENSMUSG00000070319.
GeneIDi53356.
KEGGimmu:53356.
UCSCiuc009ojm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70733 mRNA. Translation: AAD00176.1.
AF108214 mRNA. Translation: AAF14221.1.
BC008511 mRNA. Translation: AAH08511.1.
CCDSiCCDS40547.1.
RefSeqiNP_058572.2. NM_016876.3.
UniGeneiMm.427309.

3D structure databases

ProteinModelPortaliQ9Z1D1.
SMRiQ9Z1D1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207295. 5 interactors.
IntActiQ9Z1D1. 6 interactors.
MINTiMINT-1572167.
STRINGi10090.ENSMUSP00000004206.

PTM databases

iPTMnetiQ9Z1D1.
PhosphoSitePlusiQ9Z1D1.
SwissPalmiQ9Z1D1.

2D gel databases

REPRODUCTION-2DPAGEQ9Z1D1.

Proteomic databases

EPDiQ9Z1D1.
MaxQBiQ9Z1D1.
PaxDbiQ9Z1D1.
PRIDEiQ9Z1D1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004206; ENSMUSP00000004206; ENSMUSG00000070319.
GeneIDi53356.
KEGGimmu:53356.
UCSCiuc009ojm.1. mouse.

Organism-specific databases

CTDi8666.
MGIiMGI:1858258. Eif3g.

Phylogenomic databases

eggNOGiKOG0122. Eukaryota.
ENOG410Y3CW. LUCA.
GeneTreeiENSGT00510000047802.
HOGENOMiHOG000239560.
HOVERGENiHBG026850.
InParanoidiQ9Z1D1.
KOiK03248.
OMAiIVRTYKI.
OrthoDBiEOG091G0GSV.
PhylomeDBiQ9Z1D1.
TreeFamiTF101516.

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72689. Formation of a pool of free 40S subunits.
R-MMU-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

ChiTaRSiEif3g. mouse.
PROiQ9Z1D1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000070319.
ExpressionAtlasiQ9Z1D1. baseline and differential.
GenevisibleiQ9Z1D1. MM.

Family and domain databases

CDDicd12933. eIF3G. 1 hit.
Gene3Di3.30.70.330. 1 hit.
HAMAPiMF_03006. eIF3g. 1 hit.
InterProiIPR017334. eIF3_g.
IPR024675. eIF3g_N.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR10352. PTHR10352. 2 hits.
PfamiPF12353. eIF3g. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037949. Transl_init_eIF-3_RNA-bind. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3G_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1D1
Secondary accession number(s): Q9R079
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: September 19, 2002
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.