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Q9Z1B8

- PHF1_MOUSE

UniProt

Q9Z1B8 - PHF1_MOUSE

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Protein
PHD finger protein 1
Gene
Phf1, Plc1, Tctex-3, Tctex3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci (1 Publication). According to other reports, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2. Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri87 – 14256PHD-type 1
Add
BLAST
Zinc fingeri186 – 24055PHD-type 2
Add
BLAST

GO - Molecular functioni

  1. methylated histone binding Source: UniProtKB
  2. protein binding Source: UniProtKB
  3. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: UniProtKB
  2. chromatin modification Source: UniProtKB-KW
  3. negative regulation of histone H3-K27 methylation Source: UniProtKB
  4. positive regulation of histone H3-K27 methylation Source: UniProtKB
  5. regulation of transcription, DNA-templated Source: UniProtKB-KW
  6. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

DNA damage, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_222475. PRC2 methylates histones and DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
PHD finger protein 1
Short name:
Protein PHF1
Alternative name(s):
Polycomb-like protein 1
Short name:
mPCl1
T-complex testis-expressed 3
Gene namesi
Name:Phf1
Synonyms:Plc1, Tctex-3, Tctex3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:98647. Phf1.

Subcellular locationi

Nucleus. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
Note: Localizes specifically to the promoters of numerous target genes. Localizes to double-strand breaks (DSBs) sites following DNA damage. Colocalizes with NEK6 in the centrosome By similarity.1 Publication

GO - Cellular componenti

  1. ESC/E(Z) complex Source: Ensembl
  2. cytoplasm Source: UniProtKB-KW
  3. microtubule organizing center Source: UniProtKB-SubCell
  4. nucleus Source: UniProtKB
  5. site of double-strand break Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 559559PHD finger protein 1
PRO_0000059289Add
BLAST

Proteomic databases

PaxDbiQ9Z1B8.
PRIDEiQ9Z1B8.

PTM databases

PhosphoSiteiQ9Z1B8.

Expressioni

Tissue specificityi

Testis-specific.

Gene expression databases

BgeeiQ9Z1B8.
CleanExiMM_PHF1.
GenevestigatoriQ9Z1B8.

Interactioni

Subunit structurei

Associated component of the PRC2 complex. Interacts with p53/TP53 By similarity. Interacts with CHMP1.1 Publication

Protein-protein interaction databases

BioGridi204083. 2 interactions.
IntActiQ9Z1B8. 1 interaction.
MINTiMINT-4107427.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1B8.
SMRiQ9Z1B8. Positions 28-147, 186-240.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 8658Tudor
Add
BLAST

Domaini

The Tudor domain recognizes and binds H3K36me3 (1 Publication).

Sequence similaritiesi

Belongs to the Polycomblike family.
Contains 1 Tudor domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG244542.
GeneTreeiENSGT00390000009222.
HOGENOMiHOG000010307.
HOVERGENiHBG004755.
InParanoidiQ9Z1B8.
KOiK11467.
OMAiGEPPDRS.
OrthoDBiEOG73V6JJ.
PhylomeDBiQ9Z1B8.
TreeFamiTF106420.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR025894. Mtf2_C_dom.
IPR002999. Tudor.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF14061. Mtf2_C. 1 hit.
PF00628. PHD. 1 hit.
[Graphical view]
SMARTiSM00249. PHD. 2 hits.
SM00333. TUDOR. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 2 hits.
PROSITEiPS01359. ZF_PHD_1. 2 hits.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z1B8-1 [UniParc]FASTAAdd to Basket

« Hide

MAQLPRLSRL GAPSLWDPAS PAPTSGPRPR LWEGQDVLAR WTDGLLYLGT    50
IKKVDSAREV CLVQFEDDSQ FLVLWKDISP AALPGEELLC CVCRSETVVP 100
GNRLVSCEKC RHAYHQDCHV PRAPAPGEGE GASWVCRQCV FAIATKRGGA 150
LKKGPYARAM LGMKLSLPYG LKGLDWDAGH LSNRQQSYCY CGGPGEWNLK 200
MLQCRSCLQW FHEACTQCLS KPLLYGDRFY EFECCVCRGG PEKVRRLQLR 250
WVDVAHLVLY HLSVCCKKKY FDFDREILPF TSENWDSLLL GELSDTPKGE 300
RSSQLLSALN SHKDRFISGR EIKKRKCLFG LHARTPPPVE LLTGDGAPTS 350
FPSGQGPGGG VSRPLGKRWR SEPEPLRRRQ KGKVEELGPP TAAHSRHGSR 400
EQRALQASVS PPPPSPNQSY EGSSGYNFRP TDARCLPSSP IRMFASFHPS 450
ASTAGTSGDS EPPDRSPLGL HIGFPTDTPK SSPHSVTASS SSVPALTPGF 500
SRHSPPSPLC RSLSPGTGGG VRGGVSYLSR GDPVRVLARR VRPDGSVQYL 550
VEWGGGGIF 559
Length:559
Mass (Da):61,140
Last modified:December 1, 2000 - v2
Checksum:i6D2EE5F53D6164C2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti249 – 2491L → F in AAD00518. 1 Publication
Sequence conflicti344 – 3441G → E in BAA25074. 1 Publication
Sequence conflicti377 – 3771R → S in BAA25074. 1 Publication
Sequence conflicti555 – 5551G → R in BAA25074. 1 Publication
Sequence conflicti558 – 5592IF → HLPDSLLLLPSPFTHWHFHA LDL in BAA25074. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011550 mRNA. Translation: BAA25074.1.
U81490 mRNA. Translation: AAD00518.1.
CCDSiCCDS37518.1.
RefSeqiNP_033369.2. NM_009343.2.
UniGeneiMm.258953.

Genome annotation databases

EnsembliENSMUST00000073724; ENSMUSP00000073402; ENSMUSG00000024193.
GeneIDi21652.
KEGGimmu:21652.
UCSCiuc008bes.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB011550 mRNA. Translation: BAA25074.1 .
U81490 mRNA. Translation: AAD00518.1 .
CCDSi CCDS37518.1.
RefSeqi NP_033369.2. NM_009343.2.
UniGenei Mm.258953.

3D structure databases

ProteinModelPortali Q9Z1B8.
SMRi Q9Z1B8. Positions 28-147, 186-240.
ModBasei Search...

Protein-protein interaction databases

BioGridi 204083. 2 interactions.
IntActi Q9Z1B8. 1 interaction.
MINTi MINT-4107427.

PTM databases

PhosphoSitei Q9Z1B8.

Proteomic databases

PaxDbi Q9Z1B8.
PRIDEi Q9Z1B8.

Protocols and materials databases

DNASUi 21652.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000073724 ; ENSMUSP00000073402 ; ENSMUSG00000024193 .
GeneIDi 21652.
KEGGi mmu:21652.
UCSCi uc008bes.1. mouse.

Organism-specific databases

CTDi 5252.
MGIi MGI:98647. Phf1.

Phylogenomic databases

eggNOGi NOG244542.
GeneTreei ENSGT00390000009222.
HOGENOMi HOG000010307.
HOVERGENi HBG004755.
InParanoidi Q9Z1B8.
KOi K11467.
OMAi GEPPDRS.
OrthoDBi EOG73V6JJ.
PhylomeDBi Q9Z1B8.
TreeFami TF106420.

Enzyme and pathway databases

Reactomei REACT_222475. PRC2 methylates histones and DNA.

Miscellaneous databases

NextBioi 300920.
PROi Q9Z1B8.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z1B8.
CleanExi MM_PHF1.
Genevestigatori Q9Z1B8.

Family and domain databases

Gene3Di 3.30.40.10. 2 hits.
InterProi IPR025894. Mtf2_C_dom.
IPR002999. Tudor.
IPR019786. Zinc_finger_PHD-type_CS.
IPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF14061. Mtf2_C. 1 hit.
PF00628. PHD. 1 hit.
[Graphical view ]
SMARTi SM00249. PHD. 2 hits.
SM00333. TUDOR. 1 hit.
[Graphical view ]
SUPFAMi SSF57903. SSF57903. 2 hits.
PROSITEi PS01359. ZF_PHD_1. 2 hits.
PS50016. ZF_PHD_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Tctex3, related to Drosophila polycomblike, is expressed in male germ cells and mapped to the mouse T-complex."
    Kawakami S., Mitsunaga K., Kikuti Y.Y., Ando A., Inoko H., Yamamura K., Abe K.
    Mamm. Genome 9:874-880(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "Misexpression of Polycomb-group proteins in Xenopus alters anterior neural development and represses neural target genes."
    Yoshitake Y., Howard T.L., Christian J.L., Hollenberg S.M.
    Dev. Biol. 215:375-387(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: NIH Swiss.
  3. "CHMP1 is a novel nuclear matrix protein affecting chromatin structure and cell-cycle progression."
    Stauffer D.R., Howard T.L., Nyun T., Hollenberg S.M.
    J. Cell Sci. 114:2383-2393(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHMP1.
  4. "Role of hPHF1 in H3K27 methylation and Hox gene silencing."
    Cao R., Wang H., He J., Erdjument-Bromage H., Tempst P., Zhang Y.
    Mol. Cell. Biol. 28:1862-1872(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: H3K36ME3-BINDING.

Entry informationi

Entry nameiPHF1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1B8
Secondary accession number(s): O54808
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: September 3, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi