Q9Z1B5 (MD2L1_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified July 9, 2014. Version 109. History...
Names and origin
|Protein names||Recommended name:|
Mitotic spindle assembly checkpoint protein MAD2A
Mitotic arrest deficient 2-like protein 1
Short name=MAD2-like protein 1
|Organism||Mus musculus (Mouse) [Reference proteome]|
|Taxonomic identifier||10090 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus|
|Sequence length||205 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate By similarity.
Monomer and homodimer. Heterotetramer with MAD1L1. Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer. Interacts with CDC20, MAD2L1BP and with ADAM17/TACE. Dimeric MAD2L1 in the closed conformation interacts with CDC20. Monomeric MAD2L1 in the open conformation does not interact with CDC20. CDC20 competes with MAD1L1 for MAD2L1 binding. Interacts with TPR. Binds to UBD during mitosis. Kinetochore association is repressed by UBD By similarity. Interacts with NEK2 By similarity.
Nucleus By similarity. Chromosome › centromere › kinetochore By similarity. Cytoplasm By similarity. Cytoplasm › cytoskeleton › spindle pole By similarity. Note: Recruited by MAD1L1 to unattached kinetochores By similarity. Recruited to the nuclear pore complex by TPR during interphase By similarity. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2 By similarity.
The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20 By similarity.
Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2 By similarity.
Belongs to the MAD2 family.
Contains 1 HORMA domain.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Initiator methionine||1||1||Removed By similarity|
|Chain||2 – 205||204||Mitotic spindle assembly checkpoint protein MAD2A||PRO_0000126118|
|Domain||14 – 197||184||HORMA|
|Region||195 – 205||11||Required for assuming the closed conformation and for interaction with CDC20 By similarity|
Amino acid modifications
|Modified residue||2||1||N-acetylalanine By similarity|
|Modified residue||170||1||Phosphoserine By similarity|
|Modified residue||195||1||Phosphoserine By similarity|
|Sequence conflict||157||1||T → A in AAD09238. Ref.1|
|Sequence conflict||178||1||C → S in AAD09238. Ref.1|
|Sequence conflict||201||1||T → I in AAD09238. Ref.1|
|||"Identification of a novel component of the spindle assembly checkpoint in mammalian cells."|
Jin D.-Y., Jeang K.-T.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|||"Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2."|
Dobles M., Liberal V., Scott M.L., Benezra R., Sorger P.K.
Cell 101:635-645(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
|||"The transcriptional landscape of the mammalian genome."|
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
|+||Additional computationally mapped references.|
|U83902 mRNA. Translation: AAD09238.1.|
AF261919 Genomic DNA. Translation: AAF69525.1.
AK082934 mRNA. Translation: BAC38700.1.
|RefSeq||NP_062372.2. NM_019499.4. |
3D structure databases
|SMR||Q9Z1B5. Positions 11-205. |
Protein-protein interaction databases
|BioGrid||207818. 31 interactions.|
|IntAct||Q9Z1B5. 30 interactions.|
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSMUST00000101343; ENSMUSP00000098897; ENSMUSG00000029910. |
ENSMUST00000116605; ENSMUSP00000112304; ENSMUSG00000029910.
|UCSC||uc009cen.1. mouse. |
|MGI||MGI:1860374. Mad2l1. |
Gene expression databases
Family and domain databases
|Gene3D||3.30.900.10. 1 hit. |
|InterPro||IPR003511. HORMA_DNA-bd. |
|PANTHER||PTHR11842:SF11. PTHR11842:SF11. 1 hit. |
|Pfam||PF02301. HORMA. 1 hit. |
|SUPFAM||SSF56019. SSF56019. 1 hit. |
|PROSITE||PS50815. HORMA. 1 hit. |
|Accession||Primary (citable) accession number: Q9Z1B5|
Secondary accession number(s): Q9JI53
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|