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Protein

Mitotic spindle assembly checkpoint protein MAD2A

Gene

Mad2l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic cell cycle checkpoint Source: MGI
  • mitotic sister chromatid segregation Source: MGI
  • mitotic spindle assembly checkpoint Source: MGI
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of mitotic cell cycle Source: MGI
  • negative regulation of mitotic metaphase/anaphase transition Source: MGI
  • negative regulation of protein catabolic process Source: UniProtKB
  • negative regulation of ubiquitin protein ligase activity Source: MGI
  • positive regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-141405. Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components.
R-MMU-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-MMU-141444. Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-MMU-179409. APC-Cdc20 mediated degradation of Nek2A.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle assembly checkpoint protein MAD2A
Alternative name(s):
Mitotic arrest deficient 2-like protein 1
Short name:
MAD2-like protein 1
Gene namesi
Name:Mad2l1
Synonyms:Mad2a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1860374. Mad2l1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001261182 – 205Mitotic spindle assembly checkpoint protein MAD2AAdd BLAST204

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei130PhosphoserineBy similarity1
Modified residuei170PhosphoserineBy similarity1
Modified residuei185PhosphoserineBy similarity1
Modified residuei195PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. Phosphorylated by NEK2 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z1B5.
MaxQBiQ9Z1B5.
PaxDbiQ9Z1B5.
PRIDEiQ9Z1B5.
TopDownProteomicsiQ9Z1B5.

PTM databases

iPTMnetiQ9Z1B5.
PhosphoSitePlusiQ9Z1B5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029910.
ExpressionAtlasiQ9Z1B5. baseline and differential.
GenevisibleiQ9Z1B5. MM.

Interactioni

Subunit structurei

Monomer and homodimer. Heterotetramer with MAD1L1. Formation of a heterotetrameric core complex containing two molecules each of MAD1L1 and of MAD2L1 promotes binding of another molecule of MAD2L1 to each MAD2L1, resulting in a heterohexamer. Interacts with CDC20, MAD2L1BP and with ADAM17/TACE. Dimeric MAD2L1 in the closed conformation interacts with CDC20. Monomeric MAD2L1 in the open conformation does not interact with CDC20. CDC20 competes with MAD1L1 for MAD2L1 binding. Interacts with TPR. Binds to UBD (via ubiquitin-like 1 domain) during mitosis. Kinetochore association is repressed by UBD (By similarity). Interacts with NEK2 (By similarity). Interacts with HSF1; this interaction occurs in mitosis (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Sgo2Q7TSY83EBI-2552918,EBI-2552468

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207818. 106 interactors.
IntActiQ9Z1B5. 109 interactors.
MINTiMINT-123716.
STRINGi10090.ENSMUSP00000098897.

Structurei

3D structure databases

ProteinModelPortaliQ9Z1B5.
SMRiQ9Z1B5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 197HORMAPROSITE-ProRule annotationAdd BLAST184

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 205Required for assuming the closed conformation and for interaction with CDC20By similarityAdd BLAST11

Domaini

The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20 (By similarity).By similarity

Sequence similaritiesi

Belongs to the MAD2 family.Curated

Phylogenomic databases

eggNOGiKOG3285. Eukaryota.
ENOG410XSD7. LUCA.
GeneTreeiENSGT00390000007908.
HOGENOMiHOG000199586.
HOVERGENiHBG105691.
InParanoidiQ9Z1B5.
KOiK02537.
OMAiSEVPVEW.
OrthoDBiEOG091G0JBM.
PhylomeDBiQ9Z1B5.
TreeFamiTF101084.

Family and domain databases

InterProiView protein in InterPro
IPR003511. HORMA_dom.
IPR027097. Mad2.
PANTHERiPTHR11842:SF12. PTHR11842:SF12. 1 hit.
PfamiView protein in Pfam
PF02301. HORMA. 1 hit.
SUPFAMiSSF56019. SSF56019. 1 hit.
PROSITEiView protein in PROSITE
PS50815. HORMA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z1B5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQQLAREQG ITLRGSAEIV AEFFSFGINS ILYQRGIYPS ETFTRVQKYG
60 70 80 90 100
LTLLTTTDPE LIKYLNNVVE QLKEWLYKCS VQKLVVVISN IESGEVLERW
110 120 130 140 150
QFDIECDKTA KEEGVRREKS QKAIQDEIRS VIRQITATVT FLPLLEVSCS
160 170 180 190 200
FDLLIYTDKD LVVPEKWEES GPQFITNCEE VRLRSFTTTI HKVNSMVAYK

TPVND
Length:205
Mass (Da):23,598
Last modified:January 11, 2001 - v2
Checksum:iA9F3F28BC4C9738E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti157T → A in AAD09238 (Ref. 1) Curated1
Sequence conflicti178C → S in AAD09238 (Ref. 1) Curated1
Sequence conflicti201T → I in AAD09238 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83902 mRNA. Translation: AAD09238.1.
AF261919 Genomic DNA. Translation: AAF69525.1.
AK082934 mRNA. Translation: BAC38700.1.
CCDSiCCDS20210.1.
RefSeqiNP_062372.2. NM_019499.4.
XP_006506469.1. XM_006506406.3.
UniGeneiMm.489653.

Genome annotation databases

EnsembliENSMUST00000101343; ENSMUSP00000098897; ENSMUSG00000029910.
ENSMUST00000116605; ENSMUSP00000112304; ENSMUSG00000029910.
GeneIDi56150.
KEGGimmu:56150.
UCSCiuc009cen.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiMD2L1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z1B5
Secondary accession number(s): Q9JI53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: September 27, 2017
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families