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Q9Z1B2

- GSTM5_RAT

UniProt

Q9Z1B2 - GSTM5_RAT

Protein

Glutathione S-transferase Mu 5

Gene

Gstm5

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Kineticsi

    1. KM=0.14 mM for glutathione1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei120 – 1201SubstrateBy similarity

    GO - Molecular functioni

    1. glutathione transferase activity Source: MGI
    2. protein homodimerization activity Source: MGI

    GO - Biological processi

    1. glutathione metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase Mu 5 (EC:2.5.1.18)
    Alternative name(s):
    GST class-mu 5
    Gene namesi
    Name:Gstm5
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi61964. Gstm5.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 225225Glutathione S-transferase Mu 5PRO_0000271421Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.Curated

    Proteomic databases

    PaxDbiQ9Z1B2.
    PRIDEiQ9Z1B2.

    2D gel databases

    World-2DPAGE0004:Q9Z1B2.

    Expressioni

    Tissue specificityi

    Expressed in testis and brain. Very low expression in liver, kidney, heart and lung.1 Publication

    Gene expression databases

    GenevestigatoriQ9Z1B2.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000025589.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z1B2.
    SMRiQ9Z1B2. Positions 2-225.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 9288GST N-terminalAdd
    BLAST
    Domaini94 – 212119GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni11 – 122Glutathione bindingBy similarity
    Regioni50 – 545Glutathione bindingBy similarity
    Regioni63 – 642Glutathione bindingBy similarity
    Regioni76 – 772Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Mu family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiNOG300089.
    GeneTreeiENSGT00550000074559.
    HOGENOMiHOG000115735.
    HOVERGENiHBG106842.
    InParanoidiQ9Z1B2.
    KOiK00799.
    OMAiCYNSDHE.
    OrthoDBiEOG7KH9M3.
    PhylomeDBiQ9Z1B2.
    TreeFamiTF353040.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR003081. GST_mu.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    PRINTSiPR01267. GSTRNSFRASEM.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z1B2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCSKSMVLG YWDIRGLAHA IRMLLEFTDT SYEEKQYTCG EAPDYDRSQW    50
    LDVKFKLDLD FPNLPYLMDG KNKITQSNAI LRYIARKHNM CGDTEEEKIR 100
    VDIMENQIMD FRMQLVRLCY NSNHESLKPQ YLEQLPAQLK QFSLFLGKFT 150
    WFAGEKLTFV DFLTYDVLDQ NRMFEPKCLD EFPNLKAFMC RFEALEKIAA 200
    FLQSDRCFKM PINNKMAKWG NKSIC 225
    Length:225
    Mass (Da):26,629
    Last modified:January 23, 2007 - v3
    Checksum:i45A2DC2DDCE5ACA4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U86635 mRNA. Translation: AAD00603.1.
    RefSeqiNP_742035.1. NM_172038.1.
    XP_003749426.1. XM_003749378.2.
    UniGeneiRn.9158.

    Genome annotation databases

    EnsembliENSRNOT00000072342; ENSRNOP00000065389; ENSRNOG00000049743.
    ENSRNOT00000073649; ENSRNOP00000064813; ENSRNOG00000047034.
    GeneIDi100912430.
    64352.
    KEGGirno:100912430.
    rno:64352.
    UCSCiRGD:61964. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U86635 mRNA. Translation: AAD00603.1 .
    RefSeqi NP_742035.1. NM_172038.1.
    XP_003749426.1. XM_003749378.2.
    UniGenei Rn.9158.

    3D structure databases

    ProteinModelPortali Q9Z1B2.
    SMRi Q9Z1B2. Positions 2-225.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000025589.

    2D gel databases

    World-2DPAGE 0004:Q9Z1B2.

    Proteomic databases

    PaxDbi Q9Z1B2.
    PRIDEi Q9Z1B2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000072342 ; ENSRNOP00000065389 ; ENSRNOG00000049743 .
    ENSRNOT00000073649 ; ENSRNOP00000064813 ; ENSRNOG00000047034 .
    GeneIDi 100912430.
    64352.
    KEGGi rno:100912430.
    rno:64352.
    UCSCi RGD:61964. rat.

    Organism-specific databases

    CTDi 2949.
    RGDi 61964. Gstm5.

    Phylogenomic databases

    eggNOGi NOG300089.
    GeneTreei ENSGT00550000074559.
    HOGENOMi HOG000115735.
    HOVERGENi HBG106842.
    InParanoidi Q9Z1B2.
    KOi K00799.
    OMAi CYNSDHE.
    OrthoDBi EOG7KH9M3.
    PhylomeDBi Q9Z1B2.
    TreeFami TF353040.

    Miscellaneous databases

    NextBioi 613032.
    PROi Q9Z1B2.

    Gene expression databases

    Genevestigatori Q9Z1B2.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR003081. GST_mu.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01267. GSTRNSFRASEM.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rationale for reclassification of a distinctive subdivision of mammalian class Mu glutathione S-transferases that are primarily expressed in testis."
      Rowe J.D., Patskovsky Y.V., Patskovska L.N., Novikova E., Listowsky I.
      J. Biol. Chem. 273:9593-9601(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 6-27; 36-50; 57-71; 74-87; 89-120; 157-177; 179-186; 198-215 AND 219-224, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, KINETIC PARAMETERS.
      Strain: Sprague-Dawley.
      Tissue: Testis.
    2. Lubec G., Afjehi-Sadat L.
      Submitted (NOV-2006) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 157-172, INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Spinal cord.

    Entry informationi

    Entry nameiGSTM5_RAT
    AccessioniPrimary (citable) accession number: Q9Z1B2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 98 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3