Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucose-6-phosphatase 2

Gene

G6pc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + H2O = D-glucose + phosphate.1 Publication

Pathway: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateSequence Analysis
Active sitei115 – 1151Proton donorSequence Analysis
Binding sitei168 – 1681SubstrateSequence Analysis
Active sitei174 – 1741NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Gluconeogenesis

Enzyme and pathway databases

BRENDAi3.1.3.9. 3474.
ReactomeiREACT_297294. Glucose transport.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphatase 2 (EC:3.1.3.9)
Short name:
G-6-Pase 2
Short name:
G6Pase 2
Alternative name(s):
Islet-specific glucose-6-phosphatase catalytic subunit-related protein
Gene namesi
Name:G6pc2
Synonyms:Igrp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1277193. G6pc2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424LumenalSequence AnalysisAdd
BLAST
Transmembranei25 – 4521HelicalSequence AnalysisAdd
BLAST
Topological domaini46 – 5611CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei57 – 7721HelicalSequence AnalysisAdd
BLAST
Topological domaini78 – 11538LumenalSequence AnalysisAdd
BLAST
Transmembranei116 – 13621HelicalSequence AnalysisAdd
BLAST
Topological domaini137 – 14610CytoplasmicSequence Analysis
Transmembranei147 – 16721HelicalSequence AnalysisAdd
BLAST
Topological domaini168 – 1681LumenalSequence Analysis
Transmembranei169 – 18921HelicalSequence AnalysisAdd
BLAST
Topological domaini190 – 21122CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei212 – 23221HelicalSequence AnalysisAdd
BLAST
Topological domaini233 – 25220LumenalSequence AnalysisAdd
BLAST
Transmembranei253 – 27321HelicalSequence AnalysisAdd
BLAST
Topological domaini274 – 29017CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei291 – 30717HelicalSequence AnalysisAdd
BLAST
Topological domaini308 – 31811LumenalSequence AnalysisAdd
BLAST
Transmembranei319 – 33921HelicalSequence AnalysisAdd
BLAST
Topological domaini340 – 35516CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum membrane Source: MGI
  • integral component of endoplasmic reticulum membrane Source: GO_Central
  • membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are no overt anatomical or behavioral phenotype but display a mild metabolic phenotype. Upon fasting those mice exhibit a significant decrease in blood glucose and triacylglycerol compared to wild type mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Glucose-6-phosphatase 2PRO_0000334510Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi92 – 921N-linked (GlcNAc...)By similarity

Post-translational modificationi

N-glycosylated; the non-glycosylated form is more unstable and is degraded through the proteasome.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9Z186.

Expressioni

Tissue specificityi

Specifically expressed in pancreatic islet cells, in particular those of beta-cell origin. Not detected in testis, kidney, muscle, liver, lung, spleen, brain, pituitary, gastric fundus or heart.3 Publications

Developmental stagei

Initial onset of expression in the pancreas is at E12 and prominent expression is detected at E14.1 Publication

Inductioni

Up-regulated in islet cells cultured in hyperglycemic concentrations of glucose.1 Publication

Gene expression databases

BgeeiQ9Z186.
GenevisibleiQ9Z186. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005364.

Structurei

3D structure databases

ProteinModelPortaliQ9Z186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi352 – 3554Prevents secretion from ERSequence Analysis

Sequence similaritiesi

Belongs to the glucose-6-phosphatase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG82628.
GeneTreeiENSGT00510000046465.
HOGENOMiHOG000264239.
HOVERGENiHBG003560.
InParanoidiQ9Z186.
KOiK01084.
OMAiLTWSFLW.
OrthoDBiEOG73NG4N.
PhylomeDBiQ9Z186.
TreeFamiTF324388.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z186-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDFLHRSGVL IIHHLQEDYR TYYGFLNFMS NVGDPRNIFS IYFPLWFQLN
60 70 80 90 100
QNVGTKMIWV AVIGDWFNLI FKWILFGHRP YWWIQETEIY PNHSSPCLEQ
110 120 130 140 150
FPTTCETGPG SPSGHAMGSS CVWYVMVTAA LSYTISRMEE SSVTLHRLTW
160 170 180 190 200
SFLWSVFWLI QISVCISRVF IATHFPHQVI LGVIGGMLVA EAFEHTPGVH
210 220 230 240 250
MASLSVYLKT NVFLFLFALG FYLLLRLFGI DLLWSVPIAK KWCANPDWIH
260 270 280 290 300
IDSTPFAGLV RNLGVLFGLG FAINSEMFLR SCQGENGTKP SFRLLCALTS
310 320 330 340 350
LTTMQLYRFI KIPTHAEPLF YLLSFCKSAS IPLMVVALIP YCVHMLMRPG

DKKTK
Length:355
Mass (Da):40,681
Last modified:May 1, 1999 - v1
Checksum:iC814C27DE44E306A
GO
Isoform 2 (identifier: Q9Z186-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     148-154: LTWSFLW → DASSRGL
     155-355: Missing.

Show »
Length:154
Mass (Da):17,894
Checksum:i3B97B3DEE219A09C
GO

Sequence cautioni

The sequence AAI11906.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei148 – 1547LTWSFLW → DASSRGL in isoform 2. 1 PublicationVSP_033650
Alternative sequencei155 – 355201Missing in isoform 2. 1 PublicationVSP_033651Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47787 mRNA. Translation: CAA87708.1.
AF118766
, AF118762, AF118763, AF118764, AF118765 Genomic DNA. Translation: AAD28562.1.
AK148465 mRNA. Translation: BAE28569.1.
AL929170 Genomic DNA. Translation: CAM24718.1.
AL929170 Genomic DNA. Translation: CAM24719.1.
BC111905 mRNA. Translation: AAI11906.1. Sequence problems.
CCDSiCCDS16089.1. [Q9Z186-1]
CCDS71066.1. [Q9Z186-2]
RefSeqiNP_001276785.1. NM_001289856.1.
NP_001276786.1. NM_001289857.1. [Q9Z186-2]
NP_067306.1. NM_021331.4. [Q9Z186-1]
UniGeneiMm.140768.

Genome annotation databases

EnsembliENSMUST00000005364; ENSMUSP00000005364; ENSMUSG00000005232. [Q9Z186-1]
ENSMUST00000112317; ENSMUSP00000107936; ENSMUSG00000005232. [Q9Z186-2]
GeneIDi14378.
KEGGimmu:14378.
UCSCiuc008jxy.1. mouse. [Q9Z186-1]
uc008jxz.1. mouse. [Q9Z186-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47787 mRNA. Translation: CAA87708.1.
AF118766
, AF118762, AF118763, AF118764, AF118765 Genomic DNA. Translation: AAD28562.1.
AK148465 mRNA. Translation: BAE28569.1.
AL929170 Genomic DNA. Translation: CAM24718.1.
AL929170 Genomic DNA. Translation: CAM24719.1.
BC111905 mRNA. Translation: AAI11906.1. Sequence problems.
CCDSiCCDS16089.1. [Q9Z186-1]
CCDS71066.1. [Q9Z186-2]
RefSeqiNP_001276785.1. NM_001289856.1.
NP_001276786.1. NM_001289857.1. [Q9Z186-2]
NP_067306.1. NM_021331.4. [Q9Z186-1]
UniGeneiMm.140768.

3D structure databases

ProteinModelPortaliQ9Z186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005364.

Proteomic databases

PRIDEiQ9Z186.

Protocols and materials databases

DNASUi14378.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005364; ENSMUSP00000005364; ENSMUSG00000005232. [Q9Z186-1]
ENSMUST00000112317; ENSMUSP00000107936; ENSMUSG00000005232. [Q9Z186-2]
GeneIDi14378.
KEGGimmu:14378.
UCSCiuc008jxy.1. mouse. [Q9Z186-1]
uc008jxz.1. mouse. [Q9Z186-2]

Organism-specific databases

CTDi57818.
MGIiMGI:1277193. G6pc2.

Phylogenomic databases

eggNOGiNOG82628.
GeneTreeiENSGT00510000046465.
HOGENOMiHOG000264239.
HOVERGENiHBG003560.
InParanoidiQ9Z186.
KOiK01084.
OMAiLTWSFLW.
OrthoDBiEOG73NG4N.
PhylomeDBiQ9Z186.
TreeFamiTF324388.

Enzyme and pathway databases

UniPathwayiUPA00138.
BRENDAi3.1.3.9. 3474.
ReactomeiREACT_297294. Glucose transport.

Miscellaneous databases

NextBioi285885.
PROiQ9Z186.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z186.
GenevisibleiQ9Z186. MM.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a pancreatic islet-specific glucose-6-phosphatase catalytic subunit-related protein."
    Arden S.D., Zahn T., Steegers S., Webb S., Bergman B., O'Brien R.M., Hutton J.C.
    Diabetes 48:531-542(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, GLYCOSYLATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Pancreatic islet.
  2. "Structure and promoter activity of an islet-specific glucose-6-phosphatase catalytic subunit-related gene."
    Ebert D.H., Bischof L.J., Streeper R.S., Chapman S.C., Svitek C.A., Goldman J.K., Mathews C.E., Leiter E.H., Hutton J.C., O'Brien R.M.
    Diabetes 48:543-551(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Pancreas.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  6. "Evidence for the expression of both the hydrolase and translocase components of hepatic glucose-6-phosphatase in murine pancreatic islets."
    Goh B.-H., Efendic S., Khan A., Portwood N.
    Biochem. Biophys. Res. Commun. 307:935-941(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Identification of the beta cell antigen targeted by a prevalent population of pathogenic CD8+ T cells in autoimmune diabetes."
    Lieberman S.M., Evans A.M., Han B., Takaki T., Vinnitskaya Y., Caldwell J.A., Serreze D.V., Shabanowitz J., Hunt D.F., Nathenson S.G., Santamaria P., DiLorenzo T.P.
    Proc. Natl. Acad. Sci. U.S.A. 100:8384-8388(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION AS AN AUTOANTIGEN.
  8. "Enzymatic characterization of the pancreatic islet-specific glucose-6-phosphatase-related protein (IGRP)."
    Petrolonis A.J., Yang Q., Tummino P.J., Fish S.M., Prack A.E., Jain S., Parsons T.F., Li P., Dales N.A., Ge L., Langston S.P., Schuller A.G.P., An W.F., Tartaglia L.A., Chen H., Hong S.-B.
    J. Biol. Chem. 279:13976-13983(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, INDUCTION, TISSUE SPECIFICITY.
  9. "Identification of CD4+ T cell-specific epitopes of islet-specific glucose-6-phosphatase catalytic subunit-related protein: a novel beta cell autoantigen in type 1 diabetes."
    Mukherjee R., Wagar D., Stephens T.A., Lee-Chan E., Singh B.
    J. Immunol. 174:5306-5315(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS AN AUTOANTIGEN.
  10. "Deletion of the gene encoding the islet-specific glucose-6-phosphatase catalytic subunit-related protein autoantigen results in a mild metabolic phenotype."
    Wang Y., Martin C.C., Oeser J.K., Sarkar S., McGuinness O.P., Hutton J.C., O'Brien R.M.
    Diabetologia 50:774-778(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiG6PC2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z186
Secondary accession number(s): A2AUN2, Q2M2M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 1999
Last modified: June 24, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

G6pc2 is an autoantigen which is the natural target of a prevalent T-cell population causing insulin-dependent diabetes mellitus through destruction of pancreatic beta cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.