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Q9Z186

- G6PC2_MOUSE

UniProt

Q9Z186 - G6PC2_MOUSE

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Protein
Glucose-6-phosphatase 2
Gene
G6pc2, Igrp
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis By similarity.

Catalytic activityi

D-glucose 6-phosphate + H2O = D-glucose + phosphate.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791Substrate Reviewed prediction
Active sitei115 – 1151Proton donor Reviewed prediction
Binding sitei168 – 1681Substrate Reviewed prediction
Active sitei174 – 1741Nucleophile By similarity

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-UniPathway
  2. glucose homeostasis Source: Ensembl
  3. regulation of insulin secretion Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Gluconeogenesis

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphatase 2 (EC:3.1.3.9)
Short name:
G-6-Pase 2
Short name:
G6Pase 2
Alternative name(s):
Islet-specific glucose-6-phosphatase catalytic subunit-related protein
Gene namesi
Name:G6pc2
Synonyms:Igrp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1277193. G6pc2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424Lumenal Reviewed prediction
Add
BLAST
Transmembranei25 – 4521Helical; Reviewed prediction
Add
BLAST
Topological domaini46 – 5611Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei57 – 7721Helical; Reviewed prediction
Add
BLAST
Topological domaini78 – 11538Lumenal Reviewed prediction
Add
BLAST
Transmembranei116 – 13621Helical; Reviewed prediction
Add
BLAST
Topological domaini137 – 14610Cytoplasmic Reviewed prediction
Transmembranei147 – 16721Helical; Reviewed prediction
Add
BLAST
Topological domaini168 – 1681Lumenal Reviewed prediction
Transmembranei169 – 18921Helical; Reviewed prediction
Add
BLAST
Topological domaini190 – 21122Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei212 – 23221Helical; Reviewed prediction
Add
BLAST
Topological domaini233 – 25220Lumenal Reviewed prediction
Add
BLAST
Transmembranei253 – 27321Helical; Reviewed prediction
Add
BLAST
Topological domaini274 – 29017Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei291 – 30717Helical; Reviewed prediction
Add
BLAST
Topological domaini308 – 31811Lumenal Reviewed prediction
Add
BLAST
Transmembranei319 – 33921Helical; Reviewed prediction
Add
BLAST
Topological domaini340 – 35516Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. endoplasmic reticulum membrane Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are no overt anatomical or behavioral phenotype but display a mild metabolic phenotype. Upon fasting those mice exhibit a significant decrease in blood glucose and triacylglycerol compared to wild type mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Glucose-6-phosphatase 2
PRO_0000334510Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi92 – 921N-linked (GlcNAc...) By similarity

Post-translational modificationi

N-glycosylated; the non-glycosylated form is more unstable and is degraded through the proteasome By similarity.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9Z186.

Expressioni

Tissue specificityi

Specifically expressed in pancreatic islet cells, in particular those of beta-cell origin. Not detected in testis, kidney, muscle, liver, lung, spleen, brain, pituitary, gastric fundus or heart.3 Publications

Developmental stagei

Initial onset of expression in the pancreas is at E12 and prominent expression is detected at E14.1 Publication

Inductioni

Up-regulated in islet cells cultured in hyperglycemic concentrations of glucose.1 Publication

Gene expression databases

BgeeiQ9Z186.
GenevestigatoriQ9Z186.

Structurei

3D structure databases

ProteinModelPortaliQ9Z186.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi352 – 3554Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG82628.
GeneTreeiENSGT00510000046465.
HOGENOMiHOG000264239.
HOVERGENiHBG003560.
InParanoidiQ9Z186.
KOiK01084.
OMAiLIQISVC.
OrthoDBiEOG73NG4N.
PhylomeDBiQ9Z186.
TreeFamiTF324388.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9Z186-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDFLHRSGVL IIHHLQEDYR TYYGFLNFMS NVGDPRNIFS IYFPLWFQLN    50
QNVGTKMIWV AVIGDWFNLI FKWILFGHRP YWWIQETEIY PNHSSPCLEQ 100
FPTTCETGPG SPSGHAMGSS CVWYVMVTAA LSYTISRMEE SSVTLHRLTW 150
SFLWSVFWLI QISVCISRVF IATHFPHQVI LGVIGGMLVA EAFEHTPGVH 200
MASLSVYLKT NVFLFLFALG FYLLLRLFGI DLLWSVPIAK KWCANPDWIH 250
IDSTPFAGLV RNLGVLFGLG FAINSEMFLR SCQGENGTKP SFRLLCALTS 300
LTTMQLYRFI KIPTHAEPLF YLLSFCKSAS IPLMVVALIP YCVHMLMRPG 350
DKKTK 355
Length:355
Mass (Da):40,681
Last modified:May 1, 1999 - v1
Checksum:iC814C27DE44E306A
GO
Isoform 2 (identifier: Q9Z186-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     148-154: LTWSFLW → DASSRGL
     155-355: Missing.

Show »
Length:154
Mass (Da):17,894
Checksum:i3B97B3DEE219A09C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei148 – 1547LTWSFLW → DASSRGL in isoform 2.
VSP_033650
Alternative sequencei155 – 355201Missing in isoform 2.
VSP_033651Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z47787 mRNA. Translation: CAA87708.1.
AF118766
, AF118762, AF118763, AF118764, AF118765 Genomic DNA. Translation: AAD28562.1.
AK148465 mRNA. Translation: BAE28569.1.
AL929170 Genomic DNA. Translation: CAM24718.1.
AL929170 Genomic DNA. Translation: CAM24719.1.
BC111905 mRNA. Translation: AAI11906.1. Sequence problems.
CCDSiCCDS16089.1. [Q9Z186-1]
CCDS71066.1. [Q9Z186-2]
RefSeqiNP_001276785.1. NM_001289856.1.
NP_001276786.1. NM_001289857.1. [Q9Z186-2]
NP_067306.1. NM_021331.4. [Q9Z186-1]
UniGeneiMm.140768.

Genome annotation databases

EnsembliENSMUST00000005364; ENSMUSP00000005364; ENSMUSG00000005232. [Q9Z186-1]
ENSMUST00000112317; ENSMUSP00000107936; ENSMUSG00000005232. [Q9Z186-2]
GeneIDi14378.
KEGGimmu:14378.
UCSCiuc008jxy.1. mouse. [Q9Z186-1]
uc008jxz.1. mouse. [Q9Z186-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z47787 mRNA. Translation: CAA87708.1 .
AF118766
, AF118762 , AF118763 , AF118764 , AF118765 Genomic DNA. Translation: AAD28562.1 .
AK148465 mRNA. Translation: BAE28569.1 .
AL929170 Genomic DNA. Translation: CAM24718.1 .
AL929170 Genomic DNA. Translation: CAM24719.1 .
BC111905 mRNA. Translation: AAI11906.1 . Sequence problems.
CCDSi CCDS16089.1. [Q9Z186-1 ]
CCDS71066.1. [Q9Z186-2 ]
RefSeqi NP_001276785.1. NM_001289856.1.
NP_001276786.1. NM_001289857.1. [Q9Z186-2 ]
NP_067306.1. NM_021331.4. [Q9Z186-1 ]
UniGenei Mm.140768.

3D structure databases

ProteinModelPortali Q9Z186.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9Z186.

Protocols and materials databases

DNASUi 14378.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000005364 ; ENSMUSP00000005364 ; ENSMUSG00000005232 . [Q9Z186-1 ]
ENSMUST00000112317 ; ENSMUSP00000107936 ; ENSMUSG00000005232 . [Q9Z186-2 ]
GeneIDi 14378.
KEGGi mmu:14378.
UCSCi uc008jxy.1. mouse. [Q9Z186-1 ]
uc008jxz.1. mouse. [Q9Z186-2 ]

Organism-specific databases

CTDi 57818.
MGIi MGI:1277193. G6pc2.

Phylogenomic databases

eggNOGi NOG82628.
GeneTreei ENSGT00510000046465.
HOGENOMi HOG000264239.
HOVERGENi HBG003560.
InParanoidi Q9Z186.
KOi K01084.
OMAi LIQISVC.
OrthoDBi EOG73NG4N.
PhylomeDBi Q9Z186.
TreeFami TF324388.

Enzyme and pathway databases

UniPathwayi UPA00138 .

Miscellaneous databases

NextBioi 285885.
PROi Q9Z186.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z186.
Genevestigatori Q9Z186.

Family and domain databases

Gene3Di 1.20.144.10. 1 hit.
InterProi IPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view ]
Pfami PF01569. PAP2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTi SM00014. acidPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF48317. SSF48317. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a pancreatic islet-specific glucose-6-phosphatase catalytic subunit-related protein."
    Arden S.D., Zahn T., Steegers S., Webb S., Bergman B., O'Brien R.M., Hutton J.C.
    Diabetes 48:531-542(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, GLYCOSYLATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Pancreatic islet.
  2. "Structure and promoter activity of an islet-specific glucose-6-phosphatase catalytic subunit-related gene."
    Ebert D.H., Bischof L.J., Streeper R.S., Chapman S.C., Svitek C.A., Goldman J.K., Mathews C.E., Leiter E.H., Hutton J.C., O'Brien R.M.
    Diabetes 48:543-551(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Pancreas.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  6. "Evidence for the expression of both the hydrolase and translocase components of hepatic glucose-6-phosphatase in murine pancreatic islets."
    Goh B.-H., Efendic S., Khan A., Portwood N.
    Biochem. Biophys. Res. Commun. 307:935-941(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  7. "Identification of the beta cell antigen targeted by a prevalent population of pathogenic CD8+ T cells in autoimmune diabetes."
    Lieberman S.M., Evans A.M., Han B., Takaki T., Vinnitskaya Y., Caldwell J.A., Serreze D.V., Shabanowitz J., Hunt D.F., Nathenson S.G., Santamaria P., DiLorenzo T.P.
    Proc. Natl. Acad. Sci. U.S.A. 100:8384-8388(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION AS AN AUTOANTIGEN.
  8. "Enzymatic characterization of the pancreatic islet-specific glucose-6-phosphatase-related protein (IGRP)."
    Petrolonis A.J., Yang Q., Tummino P.J., Fish S.M., Prack A.E., Jain S., Parsons T.F., Li P., Dales N.A., Ge L., Langston S.P., Schuller A.G.P., An W.F., Tartaglia L.A., Chen H., Hong S.-B.
    J. Biol. Chem. 279:13976-13983(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, INDUCTION, TISSUE SPECIFICITY.
  9. "Identification of CD4+ T cell-specific epitopes of islet-specific glucose-6-phosphatase catalytic subunit-related protein: a novel beta cell autoantigen in type 1 diabetes."
    Mukherjee R., Wagar D., Stephens T.A., Lee-Chan E., Singh B.
    J. Immunol. 174:5306-5315(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS AN AUTOANTIGEN.
  10. "Deletion of the gene encoding the islet-specific glucose-6-phosphatase catalytic subunit-related protein autoantigen results in a mild metabolic phenotype."
    Wang Y., Martin C.C., Oeser J.K., Sarkar S., McGuinness O.P., Hutton J.C., O'Brien R.M.
    Diabetologia 50:774-778(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiG6PC2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z186
Secondary accession number(s): A2AUN2, Q2M2M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 1999
Last modified: September 3, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

G6pc2 is an autoantigen which is the natural target of a prevalent T-cell population causing insulin-dependent diabetes mellitus through destruction of pancreatic beta cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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