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Q9Z186 (G6PC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphatase 2

Short name=G-6-Pase 2
Short name=G6Pase 2
EC=3.1.3.9
Alternative name(s):
Islet-specific glucose-6-phosphatase catalytic subunit-related protein
Gene names
Name:G6pc2
Synonyms:Igrp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis By similarity.

Catalytic activity

D-glucose 6-phosphate + H2O = D-glucose + phosphate. Ref.8

Pathway

Carbohydrate biosynthesis; gluconeogenesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Specifically expressed in pancreatic islet cells, in particular those of beta-cell origin. Not detected in testis, kidney, muscle, liver, lung, spleen, brain, pituitary, gastric fundus or heart. Ref.1 Ref.6 Ref.8

Developmental stage

Initial onset of expression in the pancreas is at E12 and prominent expression is detected at E14. Ref.1

Induction

Up-regulated in islet cells cultured in hyperglycemic concentrations of glucose. Ref.8

Post-translational modification

N-glycosylated; the non-glycosylated form is more unstable and is degraded through the proteasome By similarity. Ref.1

Disruption phenotype

Mice are no overt anatomical or behavioral phenotype but display a mild metabolic phenotype. Upon fasting those mice exhibit a significant decrease in blood glucose and triacylglycerol compared to wild type mice. Ref.10

Miscellaneous

G6pc2 is an autoantigen which is the natural target of a prevalent T-cell population causing insulin-dependent diabetes mellitus through destruction of pancreatic beta cells.

Sequence similarities

Belongs to the glucose-6-phosphatase family.

Sequence caution

The sequence AAI11906.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Z186-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Z186-2)

The sequence of this isoform differs from the canonical sequence as follows:
     148-154: LTWSFLW → DASSRGL
     155-355: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Glucose-6-phosphatase 2
PRO_0000334510

Regions

Topological domain1 – 2424Lumenal Potential
Transmembrane25 – 4521Helical; Potential
Topological domain46 – 5611Cytoplasmic Potential
Transmembrane57 – 7721Helical; Potential
Topological domain78 – 11538Lumenal Potential
Transmembrane116 – 13621Helical; Potential
Topological domain137 – 14610Cytoplasmic Potential
Transmembrane147 – 16721Helical; Potential
Topological domain1681Lumenal Potential
Transmembrane169 – 18921Helical; Potential
Topological domain190 – 21122Cytoplasmic Potential
Transmembrane212 – 23221Helical; Potential
Topological domain233 – 25220Lumenal Potential
Transmembrane253 – 27321Helical; Potential
Topological domain274 – 29017Cytoplasmic Potential
Transmembrane291 – 30717Helical; Potential
Topological domain308 – 31811Lumenal Potential
Transmembrane319 – 33921Helical; Potential
Topological domain340 – 35516Cytoplasmic Potential
Motif352 – 3554Prevents secretion from ER Potential

Sites

Active site1151Proton donor Potential
Active site1741Nucleophile By similarity
Binding site791Substrate Potential
Binding site1681Substrate Potential

Amino acid modifications

Glycosylation921N-linked (GlcNAc...) By similarity

Natural variations

Alternative sequence148 – 1547LTWSFLW → DASSRGL in isoform 2.
VSP_033650
Alternative sequence155 – 355201Missing in isoform 2.
VSP_033651

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: C814C27DE44E306A

FASTA35540,681
        10         20         30         40         50         60 
MDFLHRSGVL IIHHLQEDYR TYYGFLNFMS NVGDPRNIFS IYFPLWFQLN QNVGTKMIWV 

        70         80         90        100        110        120 
AVIGDWFNLI FKWILFGHRP YWWIQETEIY PNHSSPCLEQ FPTTCETGPG SPSGHAMGSS 

       130        140        150        160        170        180 
CVWYVMVTAA LSYTISRMEE SSVTLHRLTW SFLWSVFWLI QISVCISRVF IATHFPHQVI 

       190        200        210        220        230        240 
LGVIGGMLVA EAFEHTPGVH MASLSVYLKT NVFLFLFALG FYLLLRLFGI DLLWSVPIAK 

       250        260        270        280        290        300 
KWCANPDWIH IDSTPFAGLV RNLGVLFGLG FAINSEMFLR SCQGENGTKP SFRLLCALTS 

       310        320        330        340        350 
LTTMQLYRFI KIPTHAEPLF YLLSFCKSAS IPLMVVALIP YCVHMLMRPG DKKTK 

« Hide

Isoform 2 [UniParc].

Checksum: 3B97B3DEE219A09C
Show »

FASTA15417,894

References

« Hide 'large scale' references
[1]"Molecular cloning of a pancreatic islet-specific glucose-6-phosphatase catalytic subunit-related protein."
Arden S.D., Zahn T., Steegers S., Webb S., Bergman B., O'Brien R.M., Hutton J.C.
Diabetes 48:531-542(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, GLYCOSYLATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Pancreatic islet.
[2]"Structure and promoter activity of an islet-specific glucose-6-phosphatase catalytic subunit-related gene."
Ebert D.H., Bischof L.J., Streeper R.S., Chapman S.C., Svitek C.A., Goldman J.K., Mathews C.E., Leiter E.H., Hutton J.C., O'Brien R.M.
Diabetes 48:543-551(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Pancreas.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[6]"Evidence for the expression of both the hydrolase and translocase components of hepatic glucose-6-phosphatase in murine pancreatic islets."
Goh B.-H., Efendic S., Khan A., Portwood N.
Biochem. Biophys. Res. Commun. 307:935-941(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Identification of the beta cell antigen targeted by a prevalent population of pathogenic CD8+ T cells in autoimmune diabetes."
Lieberman S.M., Evans A.M., Han B., Takaki T., Vinnitskaya Y., Caldwell J.A., Serreze D.V., Shabanowitz J., Hunt D.F., Nathenson S.G., Santamaria P., DiLorenzo T.P.
Proc. Natl. Acad. Sci. U.S.A. 100:8384-8388(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION AS AN AUTOANTIGEN.
[8]"Enzymatic characterization of the pancreatic islet-specific glucose-6-phosphatase-related protein (IGRP)."
Petrolonis A.J., Yang Q., Tummino P.J., Fish S.M., Prack A.E., Jain S., Parsons T.F., Li P., Dales N.A., Ge L., Langston S.P., Schuller A.G.P., An W.F., Tartaglia L.A., Chen H., Hong S.-B.
J. Biol. Chem. 279:13976-13983(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY, INDUCTION, TISSUE SPECIFICITY.
[9]"Identification of CD4+ T cell-specific epitopes of islet-specific glucose-6-phosphatase catalytic subunit-related protein: a novel beta cell autoantigen in type 1 diabetes."
Mukherjee R., Wagar D., Stephens T.A., Lee-Chan E., Singh B.
J. Immunol. 174:5306-5315(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION AS AN AUTOANTIGEN.
[10]"Deletion of the gene encoding the islet-specific glucose-6-phosphatase catalytic subunit-related protein autoantigen results in a mild metabolic phenotype."
Wang Y., Martin C.C., Oeser J.K., Sarkar S., McGuinness O.P., Hutton J.C., O'Brien R.M.
Diabetologia 50:774-778(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z47787 mRNA. Translation: CAA87708.1.
AF118766 expand/collapse EMBL AC list , AF118762, AF118763, AF118764, AF118765 Genomic DNA. Translation: AAD28562.1.
AK148465 mRNA. Translation: BAE28569.1.
AL929170 Genomic DNA. Translation: CAM24718.1.
AL929170 Genomic DNA. Translation: CAM24719.1.
BC111905 mRNA. Translation: AAI11906.1. Sequence problems.
CCDSCCDS16089.1. [Q9Z186-1]
RefSeqNP_001276785.1. NM_001289856.1.
NP_001276786.1. NM_001289857.1. [Q9Z186-2]
NP_067306.1. NM_021331.4. [Q9Z186-1]
UniGeneMm.140768.

3D structure databases

ProteinModelPortalQ9Z186.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9Z186.

Protocols and materials databases

DNASU14378.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005364; ENSMUSP00000005364; ENSMUSG00000005232. [Q9Z186-1]
ENSMUST00000112317; ENSMUSP00000107936; ENSMUSG00000005232. [Q9Z186-2]
GeneID14378.
KEGGmmu:14378.
UCSCuc008jxy.1. mouse. [Q9Z186-1]
uc008jxz.1. mouse. [Q9Z186-2]

Organism-specific databases

CTD57818.
MGIMGI:1277193. G6pc2.

Phylogenomic databases

eggNOGNOG82628.
GeneTreeENSGT00510000046465.
HOGENOMHOG000264239.
HOVERGENHBG003560.
InParanoidQ9Z186.
KOK01084.
OMALIQISVC.
OrthoDBEOG73NG4N.
PhylomeDBQ9Z186.
TreeFamTF324388.

Enzyme and pathway databases

UniPathwayUPA00138.

Gene expression databases

BgeeQ9Z186.
GenevestigatorQ9Z186.

Family and domain databases

Gene3D1.20.144.10. 1 hit.
InterProIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMSSF48317. SSF48317. 1 hit.
ProtoNetSearch...

Other

NextBio285885.
PROQ9Z186.
SOURCESearch...

Entry information

Entry nameG6PC2_MOUSE
AccessionPrimary (citable) accession number: Q9Z186
Secondary accession number(s): A2AUN2, Q2M2M7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot