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Q9Z186

- G6PC2_MOUSE

UniProt

Q9Z186 - G6PC2_MOUSE

Protein

Glucose-6-phosphatase 2

Gene

G6pc2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis By similarity.By similarity

    Catalytic activityi

    D-glucose 6-phosphate + H2O = D-glucose + phosphate.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei79 – 791SubstrateSequence Analysis
    Active sitei115 – 1151Proton donorSequence Analysis
    Binding sitei168 – 1681SubstrateSequence Analysis
    Active sitei174 – 1741NucleophileBy similarity

    GO - Molecular functioni

    1. hydrolase activity Source: UniProtKB-KW

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-UniPathway
    2. glucose homeostasis Source: Ensembl
    3. regulation of insulin secretion Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Gluconeogenesis

    Enzyme and pathway databases

    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucose-6-phosphatase 2 (EC:3.1.3.9)
    Short name:
    G-6-Pase 2
    Short name:
    G6Pase 2
    Alternative name(s):
    Islet-specific glucose-6-phosphatase catalytic subunit-related protein
    Gene namesi
    Name:G6pc2
    Synonyms:Igrp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1277193. G6pc2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: MGI
    2. endoplasmic reticulum membrane Source: MGI
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice are no overt anatomical or behavioral phenotype but display a mild metabolic phenotype. Upon fasting those mice exhibit a significant decrease in blood glucose and triacylglycerol compared to wild type mice.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 355355Glucose-6-phosphatase 2PRO_0000334510Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi92 – 921N-linked (GlcNAc...)By similarity

    Post-translational modificationi

    N-glycosylated; the non-glycosylated form is more unstable and is degraded through the proteasome.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ9Z186.

    Expressioni

    Tissue specificityi

    Specifically expressed in pancreatic islet cells, in particular those of beta-cell origin. Not detected in testis, kidney, muscle, liver, lung, spleen, brain, pituitary, gastric fundus or heart.3 Publications

    Developmental stagei

    Initial onset of expression in the pancreas is at E12 and prominent expression is detected at E14.1 Publication

    Inductioni

    Up-regulated in islet cells cultured in hyperglycemic concentrations of glucose.1 Publication

    Gene expression databases

    BgeeiQ9Z186.
    GenevestigatoriQ9Z186.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z186.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2424LumenalSequence AnalysisAdd
    BLAST
    Topological domaini46 – 5611CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini78 – 11538LumenalSequence AnalysisAdd
    BLAST
    Topological domaini137 – 14610CytoplasmicSequence Analysis
    Topological domaini168 – 1681LumenalSequence Analysis
    Topological domaini190 – 21122CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini233 – 25220LumenalSequence AnalysisAdd
    BLAST
    Topological domaini274 – 29017CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini308 – 31811LumenalSequence AnalysisAdd
    BLAST
    Topological domaini340 – 35516CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei57 – 7721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei116 – 13621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei147 – 16721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei169 – 18921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei212 – 23221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei253 – 27321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei291 – 30717HelicalSequence AnalysisAdd
    BLAST
    Transmembranei319 – 33921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi352 – 3554Prevents secretion from ERSequence Analysis

    Sequence similaritiesi

    Belongs to the glucose-6-phosphatase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG82628.
    GeneTreeiENSGT00510000046465.
    HOGENOMiHOG000264239.
    HOVERGENiHBG003560.
    InParanoidiQ9Z186.
    KOiK01084.
    OMAiLIQISVC.
    OrthoDBiEOG73NG4N.
    PhylomeDBiQ9Z186.
    TreeFamiTF324388.

    Family and domain databases

    Gene3Di1.20.144.10. 1 hit.
    InterProiIPR016275. Glucose-6-phosphatase.
    IPR000326. P_Acid_Pase_2/haloperoxidase.
    [Graphical view]
    PfamiPF01569. PAP2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
    SMARTiSM00014. acidPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48317. SSF48317. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9Z186-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDFLHRSGVL IIHHLQEDYR TYYGFLNFMS NVGDPRNIFS IYFPLWFQLN    50
    QNVGTKMIWV AVIGDWFNLI FKWILFGHRP YWWIQETEIY PNHSSPCLEQ 100
    FPTTCETGPG SPSGHAMGSS CVWYVMVTAA LSYTISRMEE SSVTLHRLTW 150
    SFLWSVFWLI QISVCISRVF IATHFPHQVI LGVIGGMLVA EAFEHTPGVH 200
    MASLSVYLKT NVFLFLFALG FYLLLRLFGI DLLWSVPIAK KWCANPDWIH 250
    IDSTPFAGLV RNLGVLFGLG FAINSEMFLR SCQGENGTKP SFRLLCALTS 300
    LTTMQLYRFI KIPTHAEPLF YLLSFCKSAS IPLMVVALIP YCVHMLMRPG 350
    DKKTK 355
    Length:355
    Mass (Da):40,681
    Last modified:May 1, 1999 - v1
    Checksum:iC814C27DE44E306A
    GO
    Isoform 2 (identifier: Q9Z186-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         148-154: LTWSFLW → DASSRGL
         155-355: Missing.

    Show »
    Length:154
    Mass (Da):17,894
    Checksum:i3B97B3DEE219A09C
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei148 – 1547LTWSFLW → DASSRGL in isoform 2. 1 PublicationVSP_033650
    Alternative sequencei155 – 355201Missing in isoform 2. 1 PublicationVSP_033651Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47787 mRNA. Translation: CAA87708.1.
    AF118766
    , AF118762, AF118763, AF118764, AF118765 Genomic DNA. Translation: AAD28562.1.
    AK148465 mRNA. Translation: BAE28569.1.
    AL929170 Genomic DNA. Translation: CAM24718.1.
    AL929170 Genomic DNA. Translation: CAM24719.1.
    BC111905 mRNA. Translation: AAI11906.1. Sequence problems.
    CCDSiCCDS16089.1. [Q9Z186-1]
    CCDS71066.1. [Q9Z186-2]
    RefSeqiNP_001276785.1. NM_001289856.1.
    NP_001276786.1. NM_001289857.1. [Q9Z186-2]
    NP_067306.1. NM_021331.4. [Q9Z186-1]
    UniGeneiMm.140768.

    Genome annotation databases

    EnsembliENSMUST00000005364; ENSMUSP00000005364; ENSMUSG00000005232. [Q9Z186-1]
    ENSMUST00000112317; ENSMUSP00000107936; ENSMUSG00000005232. [Q9Z186-2]
    GeneIDi14378.
    KEGGimmu:14378.
    UCSCiuc008jxy.1. mouse. [Q9Z186-1]
    uc008jxz.1. mouse. [Q9Z186-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47787 mRNA. Translation: CAA87708.1 .
    AF118766
    , AF118762 , AF118763 , AF118764 , AF118765 Genomic DNA. Translation: AAD28562.1 .
    AK148465 mRNA. Translation: BAE28569.1 .
    AL929170 Genomic DNA. Translation: CAM24718.1 .
    AL929170 Genomic DNA. Translation: CAM24719.1 .
    BC111905 mRNA. Translation: AAI11906.1 . Sequence problems.
    CCDSi CCDS16089.1. [Q9Z186-1 ]
    CCDS71066.1. [Q9Z186-2 ]
    RefSeqi NP_001276785.1. NM_001289856.1.
    NP_001276786.1. NM_001289857.1. [Q9Z186-2 ]
    NP_067306.1. NM_021331.4. [Q9Z186-1 ]
    UniGenei Mm.140768.

    3D structure databases

    ProteinModelPortali Q9Z186.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9Z186.

    Protocols and materials databases

    DNASUi 14378.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000005364 ; ENSMUSP00000005364 ; ENSMUSG00000005232 . [Q9Z186-1 ]
    ENSMUST00000112317 ; ENSMUSP00000107936 ; ENSMUSG00000005232 . [Q9Z186-2 ]
    GeneIDi 14378.
    KEGGi mmu:14378.
    UCSCi uc008jxy.1. mouse. [Q9Z186-1 ]
    uc008jxz.1. mouse. [Q9Z186-2 ]

    Organism-specific databases

    CTDi 57818.
    MGIi MGI:1277193. G6pc2.

    Phylogenomic databases

    eggNOGi NOG82628.
    GeneTreei ENSGT00510000046465.
    HOGENOMi HOG000264239.
    HOVERGENi HBG003560.
    InParanoidi Q9Z186.
    KOi K01084.
    OMAi LIQISVC.
    OrthoDBi EOG73NG4N.
    PhylomeDBi Q9Z186.
    TreeFami TF324388.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .

    Miscellaneous databases

    NextBioi 285885.
    PROi Q9Z186.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9Z186.
    Genevestigatori Q9Z186.

    Family and domain databases

    Gene3Di 1.20.144.10. 1 hit.
    InterProi IPR016275. Glucose-6-phosphatase.
    IPR000326. P_Acid_Pase_2/haloperoxidase.
    [Graphical view ]
    Pfami PF01569. PAP2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000905. Glucose-6-phosphatase. 1 hit.
    SMARTi SM00014. acidPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48317. SSF48317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a pancreatic islet-specific glucose-6-phosphatase catalytic subunit-related protein."
      Arden S.D., Zahn T., Steegers S., Webb S., Bergman B., O'Brien R.M., Hutton J.C.
      Diabetes 48:531-542(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, GLYCOSYLATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Pancreatic islet.
    2. "Structure and promoter activity of an islet-specific glucose-6-phosphatase catalytic subunit-related gene."
      Ebert D.H., Bischof L.J., Streeper R.S., Chapman S.C., Svitek C.A., Goldman J.K., Mathews C.E., Leiter E.H., Hutton J.C., O'Brien R.M.
      Diabetes 48:543-551(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Pancreas.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    6. "Evidence for the expression of both the hydrolase and translocase components of hepatic glucose-6-phosphatase in murine pancreatic islets."
      Goh B.-H., Efendic S., Khan A., Portwood N.
      Biochem. Biophys. Res. Commun. 307:935-941(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    7. "Identification of the beta cell antigen targeted by a prevalent population of pathogenic CD8+ T cells in autoimmune diabetes."
      Lieberman S.M., Evans A.M., Han B., Takaki T., Vinnitskaya Y., Caldwell J.A., Serreze D.V., Shabanowitz J., Hunt D.F., Nathenson S.G., Santamaria P., DiLorenzo T.P.
      Proc. Natl. Acad. Sci. U.S.A. 100:8384-8388(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION AS AN AUTOANTIGEN.
    8. "Enzymatic characterization of the pancreatic islet-specific glucose-6-phosphatase-related protein (IGRP)."
      Petrolonis A.J., Yang Q., Tummino P.J., Fish S.M., Prack A.E., Jain S., Parsons T.F., Li P., Dales N.A., Ge L., Langston S.P., Schuller A.G.P., An W.F., Tartaglia L.A., Chen H., Hong S.-B.
      J. Biol. Chem. 279:13976-13983(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, INDUCTION, TISSUE SPECIFICITY.
    9. "Identification of CD4+ T cell-specific epitopes of islet-specific glucose-6-phosphatase catalytic subunit-related protein: a novel beta cell autoantigen in type 1 diabetes."
      Mukherjee R., Wagar D., Stephens T.A., Lee-Chan E., Singh B.
      J. Immunol. 174:5306-5315(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION AS AN AUTOANTIGEN.
    10. "Deletion of the gene encoding the islet-specific glucose-6-phosphatase catalytic subunit-related protein autoantigen results in a mild metabolic phenotype."
      Wang Y., Martin C.C., Oeser J.K., Sarkar S., McGuinness O.P., Hutton J.C., O'Brien R.M.
      Diabetologia 50:774-778(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiG6PC2_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z186
    Secondary accession number(s): A2AUN2, Q2M2M7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    G6pc2 is an autoantigen which is the natural target of a prevalent T-cell population causing insulin-dependent diabetes mellitus through destruction of pancreatic beta cells.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3