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Protein

Glucose-6-phosphatase 2

Gene

G6pc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May hydrolyze glucose-6-phosphate to glucose in the endoplasmic reticulum. May be responsible for glucose production through glycogenolysis and gluconeogenesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + H2O = D-glucose + phosphate.1 Publication

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79SubstrateSequence analysis1
Active sitei115Proton donorSequence analysis1
Binding sitei168SubstrateSequence analysis1
Active sitei174NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Gluconeogenesis

Enzyme and pathway databases

BRENDAi3.1.3.9. 3474.
ReactomeiR-MMU-70153. Glucose transport.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphatase 2 (EC:3.1.3.9)
Short name:
G-6-Pase 2
Short name:
G6Pase 2
Alternative name(s):
Islet-specific glucose-6-phosphatase catalytic subunit-related protein
Gene namesi
Name:G6pc2
Synonyms:Igrp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1277193. G6pc2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24LumenalSequence analysisAdd BLAST24
Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
Topological domaini46 – 56CytoplasmicSequence analysisAdd BLAST11
Transmembranei57 – 77HelicalSequence analysisAdd BLAST21
Topological domaini78 – 115LumenalSequence analysisAdd BLAST38
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Topological domaini137 – 146CytoplasmicSequence analysis10
Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
Topological domaini168LumenalSequence analysis1
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 211CytoplasmicSequence analysisAdd BLAST22
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Topological domaini233 – 252LumenalSequence analysisAdd BLAST20
Transmembranei253 – 273HelicalSequence analysisAdd BLAST21
Topological domaini274 – 290CytoplasmicSequence analysisAdd BLAST17
Transmembranei291 – 307HelicalSequence analysisAdd BLAST17
Topological domaini308 – 318LumenalSequence analysisAdd BLAST11
Transmembranei319 – 339HelicalSequence analysisAdd BLAST21
Topological domaini340 – 355CytoplasmicSequence analysisAdd BLAST16

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • endoplasmic reticulum membrane Source: MGI
  • integral component of endoplasmic reticulum membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are no overt anatomical or behavioral phenotype but display a mild metabolic phenotype. Upon fasting those mice exhibit a significant decrease in blood glucose and triacylglycerol compared to wild type mice.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003345101 – 355Glucose-6-phosphatase 2Add BLAST355

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi92N-linked (GlcNAc...)By similarity1

Post-translational modificationi

N-glycosylated; the non-glycosylated form is more unstable and is degraded through the proteasome.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9Z186.
PRIDEiQ9Z186.

PTM databases

PhosphoSitePlusiQ9Z186.

Expressioni

Tissue specificityi

Specifically expressed in pancreatic islet cells, in particular those of beta-cell origin. Not detected in testis, kidney, muscle, liver, lung, spleen, brain, pituitary, gastric fundus or heart.3 Publications

Developmental stagei

Initial onset of expression in the pancreas is at E12 and prominent expression is detected at E14.1 Publication

Inductioni

Up-regulated in islet cells cultured in hyperglycemic concentrations of glucose.1 Publication

Gene expression databases

BgeeiENSMUSG00000005232.
GenevisibleiQ9Z186. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005364.

Structurei

3D structure databases

ProteinModelPortaliQ9Z186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi352 – 355Prevents secretion from ERSequence analysis4

Sequence similaritiesi

Belongs to the glucose-6-phosphatase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDXG. Eukaryota.
ENOG4110AJ7. LUCA.
GeneTreeiENSGT00510000046465.
HOGENOMiHOG000264239.
HOVERGENiHBG003560.
InParanoidiQ9Z186.
KOiK01084.
OMAiLTWSFLW.
OrthoDBiEOG091G0AXF.
PhylomeDBiQ9Z186.
TreeFamiTF324388.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z186-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDFLHRSGVL IIHHLQEDYR TYYGFLNFMS NVGDPRNIFS IYFPLWFQLN
60 70 80 90 100
QNVGTKMIWV AVIGDWFNLI FKWILFGHRP YWWIQETEIY PNHSSPCLEQ
110 120 130 140 150
FPTTCETGPG SPSGHAMGSS CVWYVMVTAA LSYTISRMEE SSVTLHRLTW
160 170 180 190 200
SFLWSVFWLI QISVCISRVF IATHFPHQVI LGVIGGMLVA EAFEHTPGVH
210 220 230 240 250
MASLSVYLKT NVFLFLFALG FYLLLRLFGI DLLWSVPIAK KWCANPDWIH
260 270 280 290 300
IDSTPFAGLV RNLGVLFGLG FAINSEMFLR SCQGENGTKP SFRLLCALTS
310 320 330 340 350
LTTMQLYRFI KIPTHAEPLF YLLSFCKSAS IPLMVVALIP YCVHMLMRPG

DKKTK
Length:355
Mass (Da):40,681
Last modified:May 1, 1999 - v1
Checksum:iC814C27DE44E306A
GO
Isoform 2 (identifier: Q9Z186-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     148-154: LTWSFLW → DASSRGL
     155-355: Missing.

Show »
Length:154
Mass (Da):17,894
Checksum:i3B97B3DEE219A09C
GO

Sequence cautioni

The sequence AAI11906 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_033650148 – 154LTWSFLW → DASSRGL in isoform 2. 1 Publication7
Alternative sequenceiVSP_033651155 – 355Missing in isoform 2. 1 PublicationAdd BLAST201

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47787 mRNA. Translation: CAA87708.1.
AF118766
, AF118762, AF118763, AF118764, AF118765 Genomic DNA. Translation: AAD28562.1.
AK148465 mRNA. Translation: BAE28569.1.
AL929170 Genomic DNA. Translation: CAM24718.1.
AL929170 Genomic DNA. Translation: CAM24719.1.
BC111905 mRNA. Translation: AAI11906.1. Sequence problems.
CCDSiCCDS16089.1. [Q9Z186-1]
CCDS71066.1. [Q9Z186-2]
RefSeqiNP_001276785.1. NM_001289856.1.
NP_001276786.1. NM_001289857.1. [Q9Z186-2]
NP_067306.1. NM_021331.4. [Q9Z186-1]
UniGeneiMm.140768.

Genome annotation databases

EnsembliENSMUST00000005364; ENSMUSP00000005364; ENSMUSG00000005232. [Q9Z186-1]
ENSMUST00000112317; ENSMUSP00000107936; ENSMUSG00000005232. [Q9Z186-2]
GeneIDi14378.
KEGGimmu:14378.
UCSCiuc008jxy.2. mouse. [Q9Z186-1]
uc008jxz.2. mouse. [Q9Z186-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47787 mRNA. Translation: CAA87708.1.
AF118766
, AF118762, AF118763, AF118764, AF118765 Genomic DNA. Translation: AAD28562.1.
AK148465 mRNA. Translation: BAE28569.1.
AL929170 Genomic DNA. Translation: CAM24718.1.
AL929170 Genomic DNA. Translation: CAM24719.1.
BC111905 mRNA. Translation: AAI11906.1. Sequence problems.
CCDSiCCDS16089.1. [Q9Z186-1]
CCDS71066.1. [Q9Z186-2]
RefSeqiNP_001276785.1. NM_001289856.1.
NP_001276786.1. NM_001289857.1. [Q9Z186-2]
NP_067306.1. NM_021331.4. [Q9Z186-1]
UniGeneiMm.140768.

3D structure databases

ProteinModelPortaliQ9Z186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005364.

PTM databases

PhosphoSitePlusiQ9Z186.

Proteomic databases

PaxDbiQ9Z186.
PRIDEiQ9Z186.

Protocols and materials databases

DNASUi14378.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005364; ENSMUSP00000005364; ENSMUSG00000005232. [Q9Z186-1]
ENSMUST00000112317; ENSMUSP00000107936; ENSMUSG00000005232. [Q9Z186-2]
GeneIDi14378.
KEGGimmu:14378.
UCSCiuc008jxy.2. mouse. [Q9Z186-1]
uc008jxz.2. mouse. [Q9Z186-2]

Organism-specific databases

CTDi57818.
MGIiMGI:1277193. G6pc2.

Phylogenomic databases

eggNOGiENOG410IDXG. Eukaryota.
ENOG4110AJ7. LUCA.
GeneTreeiENSGT00510000046465.
HOGENOMiHOG000264239.
HOVERGENiHBG003560.
InParanoidiQ9Z186.
KOiK01084.
OMAiLTWSFLW.
OrthoDBiEOG091G0AXF.
PhylomeDBiQ9Z186.
TreeFamiTF324388.

Enzyme and pathway databases

UniPathwayiUPA00138.
BRENDAi3.1.3.9. 3474.
ReactomeiR-MMU-70153. Glucose transport.

Miscellaneous databases

PROiQ9Z186.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005232.
GenevisibleiQ9Z186. MM.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiIPR016275. Glucose-6-phosphatase.
IPR000326. P_Acid_Pase_2/haloperoxidase.
[Graphical view]
PfamiPF01569. PAP2. 1 hit.
[Graphical view]
PIRSFiPIRSF000905. Glucose-6-phosphatase. 1 hit.
SMARTiSM00014. acidPPc. 1 hit.
[Graphical view]
SUPFAMiSSF48317. SSF48317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG6PC2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z186
Secondary accession number(s): A2AUN2, Q2M2M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

G6pc2 is an autoantigen which is the natural target of a prevalent T-cell population causing insulin-dependent diabetes mellitus through destruction of pancreatic beta cells.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.