Reviewed, UniProtKB/Swiss-Prot Q9Z172 (SUMO3_MOUSE)

Names and origin

Protein names

Recommended name:
    Small ubiquitin-related modifier 3
      Short name=SUMO-3
Alternative name(s):
    Ubiquitin-like protein SMT3A
    SMT3 homolog 1

Gene names

Name:	Sumo3
Synonyms:	Smt3h1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 By similarity.
Subunit structure	Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Polymeric chains can be formed through Lys-11 cross-linking By similarity. Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function By similarity.
Sequence similarities	Belongs to the ubiquitin family. SUMO subfamily. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
PTM	Isopeptide bond Ubl conjugation
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein sumoylation Inferred from direct assay. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	SUMO ligase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Z172-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Z172-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: MSEEKPK → MTTVLAQ

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 92	92	Small ubiquitin-related modifier 3		PRO_0000035959
	Propeptide	93 – 110	18	By similarity		PRO_0000035960
Regions
	Domain	15 – 92	78	Ubiquitin-like
Amino acid modifications
	Cross-link	11		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	92		Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) By similarity
Natural variations
	Alternative sequence	1 – 7	7	MSEEKPK → MTTVLAQ in isoform 2.		VSP_021948

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2950B8B1A3F88DC8	FASTA	110	12,430
	10 20 30 40 50 60 MSEEKPKEGV KTENDHINLK VAGQDGSVVQ FKIKRHTPLS KLMKAYCERQ GLSMRQIRFR 70 80 90 100 110 FDGQPINETD TPAQLEMEDE DTIDVFQQQT GGSASRGSVP TPNRCPDLCY
	Isoform 2. Checksum: C7BD3DAAB055D5F2	FASTA	110	12,345
	10 20 30 40 50 60 MTTVLAQEGV KTENDHINLK VAGQDGSVVQ FKIKRHTPLS KLMKAYCERQ GLSMRQIRFR 70 80 90 100 110 FDGQPINETD TPAQLEMEDE DTIDVFQQQT GGSASRGSVP TPNRCPDLCY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of mouse ubiquitin-like SMT3A and SMT3B cDNAs and gene/pseudogenes." Chen A., Mannen H., Li S.S.-L. Biochem. Mol. Biol. Int. 46:1161-1174(1998) [PubMed: 9891849] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Bone marrow, Embryo, Kidney, Muellerian duct and Small intestine.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF063847 mRNA. Translation: AAC99333.1. AK012742 mRNA. Translation: BAB28442.1. AK013019 mRNA. Translation: BAB28601.1. AK148306 mRNA. Translation: BAE28469.1. AK150063 mRNA. Translation: BAE29276.1. AK150200 mRNA. Translation: BAE29374.1. AK160169 mRNA. Translation: BAE35670.1. AK162035 mRNA. Translation: BAE36692.1. AK167207 mRNA. Translation: BAE39334.1. AK168988 mRNA. Translation: BAE40788.1. AK171169 mRNA. Translation: BAE42290.1. AK170896 mRNA. Translation: BAE42100.1. BC115488 mRNA. Translation: AAI15489.1. BC115489 mRNA. Translation: AAI15490.1.
IPI	IPI00129580. IPI00652964.
RefSeq	NP_064313.1.
UniGene	Mm.24433
3D structure databases
HSSP	HSSP built from PDB template 1A5R based on UniProtKB Q93068.
SMR	Q9Z172. Positions 1-92.
ModBase	Search...
PTM databases
PhosphoSite	Q9Z172.
Proteomic databases
PRIDE	Q9Z172.
Genome annotation databases
Ensembl	ENSMUSG00000020265. Mus musculus. [Contig view]
GeneID	20610.
KEGG	mmu:20610.
Organism-specific databases
MGI	MGI:1336201. Sumo3.
Phylogenomic databases
HOGENOM	Q9Z172.
HOVERGEN	Q9Z172.
OMA	Q9Z172. NADGAEM.
Gene expression databases
ArrayExpress	Q9Z172.
Bgee	Q9Z172.
CleanEx	MM_SUMO3.
GermOnline	ENSMUSG00000020265. Mus musculus.
Family and domain databases
InterPro	IPR000626. Ubiquitin. IPR019955. Ubiquitin_supergroup. [Graphical view]
Pfam	PF00240. ubiquitin. 1 hit. [Graphical view]
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS50053. UBIQUITIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	298967.
SOURCE	Search...

Entry information

Entry name

SUMO3_MOUSE

Accession

Primary (citable) accession number: Q9Z172
Secondary accession number(s): Q14C17, Q3TBL9, Q3UDI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)