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Protein

Small ubiquitin-related modifier 3

Gene

Sumo3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Plays a role in the regulation of sumoylation status of SETX (By similarity).By similarity

GO - Molecular functioni

  • protein tag Source: GO_Central
  • SUMO transferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-3065679. SUMO is proteolytically processed.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-3232118. SUMOylation of transcription factors.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5696395. Formation of Incision Complex in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Small ubiquitin-related modifier 3Curated
Short name:
SUMO-3Curated
Alternative name(s):
SMT3 homolog 1Imported
Ubiquitin-like protein SMT3A1 Publication
Short name:
Smt3A1 Publication
Gene namesi
Name:Sumo3Imported
Synonyms:Smt3a1 Publication, Smt3h1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1336201. Sumo3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • nuclear body Source: MGI
  • nucleus Source: MGI
  • PML body Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000359591 – 92Small ubiquitin-related modifier 3Add BLAST92
PropeptideiPRO_000003596093 – 110By similarityAdd BLAST18

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki92Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Post-translational modificationi

Polymeric chains can be formed through Lys-11 cross-linking.By similarity
Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9Z172.
PaxDbiQ9Z172.
PeptideAtlasiQ9Z172.
PRIDEiQ9Z172.

PTM databases

iPTMnetiQ9Z172.
PhosphoSitePlusiQ9Z172.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020265.
CleanExiMM_SUMO3.
ExpressionAtlasiQ9Z172. baseline and differential.
GenevisibleiQ9Z172. MM.

Interactioni

Subunit structurei

Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins. Interacts with USP25 (via ts SIM domain); the interaction sumoylates USP25 and inhibits its ubiquitin hydrolyzing activity (By similarity). Interacts with ARNTL/BMAL1.By similarity1 Publication

Protein-protein interaction databases

BioGridi203358. 21 interactors.
IntActiQ9Z172. 17 interactors.
MINTiMINT-4134935.
STRINGi10090.ENSMUSP00000020501.

Structurei

3D structure databases

ProteinModelPortaliQ9Z172.
SMRiQ9Z172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 92Ubiquitin-likePROSITE-ProRule annotationAdd BLAST78

Sequence similaritiesi

Belongs to the ubiquitin family. SUMO subfamily.Curated
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1769. Eukaryota.
COG5227. LUCA.
GeneTreeiENSGT00390000018808.
HOGENOMiHOG000207495.
HOVERGENiHBG053025.
InParanoidiQ9Z172.
KOiK12160.
PhylomeDBiQ9Z172.
TreeFamiTF315116.

Family and domain databases

CDDicd01763. Sumo. 1 hit.
InterProiIPR022617. Rad60/SUMO-like_dom.
IPR033950. Sumo.
IPR027218. SUMO_chordates.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PANTHERiPTHR10562:SF10. PTHR10562:SF10. 1 hit.
PfamiPF11976. Rad60-SLD. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z172-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEEKPKEGV KTENDHINLK VAGQDGSVVQ FKIKRHTPLS KLMKAYCERQ
60 70 80 90 100
GLSMRQIRFR FDGQPINETD TPAQLEMEDE DTIDVFQQQT GGSASRGSVP
110
TPNRCPDLCY
Length:110
Mass (Da):12,430
Last modified:May 1, 1999 - v1
Checksum:i2950B8B1A3F88DC8
GO
Isoform 2 (identifier: Q9Z172-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: MSEEKPK → MTTVLAQ

Note: No experimental confirmation available.
Show »
Length:110
Mass (Da):12,345
Checksum:iC7BD3DAAB055D5F2
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0219481 – 7MSEEKPK → MTTVLAQ in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063847 mRNA. Translation: AAC99333.1.
AK012742 mRNA. Translation: BAB28442.1.
AK013019 mRNA. Translation: BAB28601.1.
AK148306 mRNA. Translation: BAE28469.1.
AK150063 mRNA. Translation: BAE29276.1.
AK150200 mRNA. Translation: BAE29374.1.
AK160169 mRNA. Translation: BAE35670.1.
AK162035 mRNA. Translation: BAE36692.1.
AK167207 mRNA. Translation: BAE39334.1.
AK168988 mRNA. Translation: BAE40788.1.
AK171169 mRNA. Translation: BAE42290.1.
AK170896 mRNA. Translation: BAE42100.1.
BC115488 mRNA. Translation: AAI15489.1.
BC115489 mRNA. Translation: AAI15490.1.
CCDSiCCDS23958.1. [Q9Z172-1]
CCDS78843.1. [Q9Z172-2]
RefSeqiNP_001288600.1. NM_001301671.1. [Q9Z172-2]
NP_001288601.1. NM_001301672.1.
NP_001288602.1. NM_001301673.1.
NP_064313.1. NM_019929.4. [Q9Z172-1]
UniGeneiMm.24433.

Genome annotation databases

EnsembliENSMUST00000020501; ENSMUSP00000020501; ENSMUSG00000020265. [Q9Z172-1]
ENSMUST00000099538; ENSMUSP00000097136; ENSMUSG00000020265. [Q9Z172-2]
GeneIDi20610.
KEGGimmu:20610.
UCSCiuc007fvy.2. mouse. [Q9Z172-1]
uc007fvz.2. mouse. [Q9Z172-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063847 mRNA. Translation: AAC99333.1.
AK012742 mRNA. Translation: BAB28442.1.
AK013019 mRNA. Translation: BAB28601.1.
AK148306 mRNA. Translation: BAE28469.1.
AK150063 mRNA. Translation: BAE29276.1.
AK150200 mRNA. Translation: BAE29374.1.
AK160169 mRNA. Translation: BAE35670.1.
AK162035 mRNA. Translation: BAE36692.1.
AK167207 mRNA. Translation: BAE39334.1.
AK168988 mRNA. Translation: BAE40788.1.
AK171169 mRNA. Translation: BAE42290.1.
AK170896 mRNA. Translation: BAE42100.1.
BC115488 mRNA. Translation: AAI15489.1.
BC115489 mRNA. Translation: AAI15490.1.
CCDSiCCDS23958.1. [Q9Z172-1]
CCDS78843.1. [Q9Z172-2]
RefSeqiNP_001288600.1. NM_001301671.1. [Q9Z172-2]
NP_001288601.1. NM_001301672.1.
NP_001288602.1. NM_001301673.1.
NP_064313.1. NM_019929.4. [Q9Z172-1]
UniGeneiMm.24433.

3D structure databases

ProteinModelPortaliQ9Z172.
SMRiQ9Z172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203358. 21 interactors.
IntActiQ9Z172. 17 interactors.
MINTiMINT-4134935.
STRINGi10090.ENSMUSP00000020501.

PTM databases

iPTMnetiQ9Z172.
PhosphoSitePlusiQ9Z172.

Proteomic databases

EPDiQ9Z172.
PaxDbiQ9Z172.
PeptideAtlasiQ9Z172.
PRIDEiQ9Z172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020501; ENSMUSP00000020501; ENSMUSG00000020265. [Q9Z172-1]
ENSMUST00000099538; ENSMUSP00000097136; ENSMUSG00000020265. [Q9Z172-2]
GeneIDi20610.
KEGGimmu:20610.
UCSCiuc007fvy.2. mouse. [Q9Z172-1]
uc007fvz.2. mouse. [Q9Z172-2]

Organism-specific databases

CTDi6612.
MGIiMGI:1336201. Sumo3.

Phylogenomic databases

eggNOGiKOG1769. Eukaryota.
COG5227. LUCA.
GeneTreeiENSGT00390000018808.
HOGENOMiHOG000207495.
HOVERGENiHBG053025.
InParanoidiQ9Z172.
KOiK12160.
PhylomeDBiQ9Z172.
TreeFamiTF315116.

Enzyme and pathway databases

ReactomeiR-MMU-3065679. SUMO is proteolytically processed.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-3232118. SUMOylation of transcription factors.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5696395. Formation of Incision Complex in GG-NER.

Miscellaneous databases

PROiQ9Z172.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020265.
CleanExiMM_SUMO3.
ExpressionAtlasiQ9Z172. baseline and differential.
GenevisibleiQ9Z172. MM.

Family and domain databases

CDDicd01763. Sumo. 1 hit.
InterProiIPR022617. Rad60/SUMO-like_dom.
IPR033950. Sumo.
IPR027218. SUMO_chordates.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PANTHERiPTHR10562:SF10. PTHR10562:SF10. 1 hit.
PfamiPF11976. Rad60-SLD. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSUMO3_MOUSE
AccessioniPrimary (citable) accession number: Q9Z172
Secondary accession number(s): Q14C17, Q3TBL9, Q3UDI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.