Q9Z172 (SUMO3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Small ubiquitin-related modifier 3
Short name=SUMO-3

Alternative name(s):
SMT3 homolog 1
Ubiquitin-like protein SMT3B
Short name=Smt3B

Gene names

Name:	Sumo3
Synonyms:	Smt3b, Smt3h1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4 By similarity.
Subunit structure	Interacts with SAE2 and UBE2I. Covalently attached to a number of proteins. Interacts with USP25 (via ts SIM domain); the interaction sumoylates USP25 and inhibits its ubiquitin hydrolyzing activity By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Polymeric chains can be formed through Lys-11 cross-linking By similarity. Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function By similarity.
Sequence similarities	Belongs to the ubiquitin family. SUMO subfamily. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from direct assay PubMed 18644859. Source: MGI protein localization to nucleus Inferred from direct assay PubMed 18644859. Source: MGI protein sumoylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear body Inferred from direct assay PubMed 18644859. Source: MGI
Molecular_function	SUMO ligase activity Inferred from direct assay PubMed 15767674PubMed 18644859. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Z172-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Z172-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: MSEEKPK → MTTVLAQ

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 92	92	Small ubiquitin-related modifier 3		PRO_0000035959
	Propeptide	93 – 110	18	By similarity		PRO_0000035960
Regions
	Domain	15 – 92	78	Ubiquitin-like
Amino acid modifications
	Cross-link	11		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	92		Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) By similarity
Natural variations
	Alternative sequence	1 – 7	7	MSEEKPK → MTTVLAQ in isoform 2.		VSP_021948

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 2950B8B1A3F88DC8	FASTA	110	12,430
	10 20 30 40 50 60 MSEEKPKEGV KTENDHINLK VAGQDGSVVQ FKIKRHTPLS KLMKAYCERQ GLSMRQIRFR 70 80 90 100 110 FDGQPINETD TPAQLEMEDE DTIDVFQQQT GGSASRGSVP TPNRCPDLCY
	Isoform 2 [UniParc]. Checksum: C7BD3DAAB055D5F2	FASTA	110	12,345
	10 20 30 40 50 60 MTTVLAQEGV KTENDHINLK VAGQDGSVVQ FKIKRHTPLS KLMKAYCERQ GLSMRQIRFR 70 80 90 100 110 FDGQPINETD TPAQLEMEDE DTIDVFQQQT GGSASRGSVP TPNRCPDLCY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of mouse ubiquitin-like SMT3A and SMT3B cDNAs and gene/pseudogenes." Chen A., Mannen H., Li S.S.-L. Biochem. Mol. Biol. Int. 46:1161-1174(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Bone marrow, Embryo, Kidney, Muellerian duct and Small intestine.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF063847 mRNA. Translation: AAC99333.1. AK012742 mRNA. Translation: BAB28442.1. AK013019 mRNA. Translation: BAB28601.1. AK148306 mRNA. Translation: BAE28469.1. AK150063 mRNA. Translation: BAE29276.1. AK150200 mRNA. Translation: BAE29374.1. AK160169 mRNA. Translation: BAE35670.1. AK162035 mRNA. Translation: BAE36692.1. AK167207 mRNA. Translation: BAE39334.1. AK168988 mRNA. Translation: BAE40788.1. AK171169 mRNA. Translation: BAE42290.1. AK170896 mRNA. Translation: BAE42100.1. BC115488 mRNA. Translation: AAI15489.1. BC115489 mRNA. Translation: AAI15490.1.
IPI	IPI00129580. IPI00652964.
RefSeq	NP_064313.1. NM_019929.3.
UniGene	Mm.24433.
3D structure databases
ProteinModelPortal	Q9Z172.
SMR	Q9Z172. Positions 14-88.
ModBase	Search...
PTM databases
PhosphoSite	Q9Z172.
Proteomic databases
PaxDb	Q9Z172.
PRIDE	Q9Z172.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020501; ENSMUSP00000020501; ENSMUSG00000020265. ENSMUST00000099538; ENSMUSP00000097136; ENSMUSG00000020265.
GeneID	20610.
KEGG	mmu:20610.
Organism-specific databases
CTD	6612.
MGI	MGI:1336201. Sumo3.
Phylogenomic databases
eggNOG	COG5227.
GeneTree	ENSGT00390000018808.
HOGENOM	HOG000207495.
HOVERGEN	HBG053025.
InParanoid	Q9Z172.
KO	K12160.
OrthoDB	EOG46HGC8.
Gene expression databases
ArrayExpress	Q9Z172.
Bgee	Q9Z172.
CleanEx	MM_SUMO3.
Genevestigator	Q9Z172.
GermOnline	ENSMUSG00000020265. Mus musculus.
Family and domain databases
InterPro	IPR022617. SUMO. IPR027218. SUMO_chordates. IPR000626. Ubiquitin. IPR019955. Ubiquitin_supergroup. [Graphical view]
PANTHER	PTHR10562:SF10. PTHR10562:SF10. 1 hit.
Pfam	PF11976. Rad60-SLD. 1 hit. [Graphical view]
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS50053. UBIQUITIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SUMO3. mouse.
NextBio	298967.
SOURCE	Search...

Entry information

Entry name

SUMO3_MOUSE

Accession

Primary (citable) accession number: Q9Z172
Secondary accession number(s): Q14C17, Q3TBL9, Q3UDI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program