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Protein

Copine-6

Gene

Cpne6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Binds phospholipid membranes in a calcium-dependent manner (PubMed:9886090). Plays a role in dendrite formation by melanocytes (By similarity).By similarity1 Publication

GO - Molecular functioni

  • phosphatidylserine binding Source: MGI

GO - Biological processi

  • cellular response to calcium ion Source: UniProtKB
  • positive regulation of dendrite extension Source: MGI

Keywordsi

Biological processDifferentiation

Enzyme and pathway databases

ReactomeiR-MMU-1483206 Glycerophospholipid biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Copine-6Curated
Alternative name(s):
Copine VIBy similarityImported
Neuronal-copine1 Publication
Short name:
N-copine1 Publication
Gene namesi
Name:Cpne6Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1334445 Cpne6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi33D → N: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-35; N-93 and N-96. 1 Publication1
Mutagenesisi35D → N: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-33; N-93 and N-96. 1 Publication1
Mutagenesisi93D → N: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-33; N-35 and N-96. 1 Publication1
Mutagenesisi96D → N: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-33; N-35 and N-93. 1 Publication1
Mutagenesisi167D → N: Inhibits strongly calcium-dependent translocation to the cell membrane. Inhibits strongly calcium-dependent translocation to the cell membrane; when associated with N-173. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-173; N-229 and N-231. 1 Publication1
Mutagenesisi173D → N: Inhibits strongly calcium-dependent translocation to the cell membrane. Inhibits strongly calcium-dependent translocation to the cell membrane; when associated with N-167. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-167; N-229 and N-231. 1 Publication1
Mutagenesisi229D → N: Inhibits strongly calcium-dependent translocation to the cell membrane; when associated with N-231. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-167; N-173 and N-231. 1 Publication1
Mutagenesisi231D → N: Does not inhibit calcium-dependent translocation to the cell membrane. Inhibits strongly calcium-dependent translocation to the cell membrane; when associated with N-229. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-167; N-173 and N-229. 1 Publication1
Mutagenesisi272K → A: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with A-273 and A-274. 1 Publication1
Mutagenesisi273K → A: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with A-272 and A-274. 1 Publication1
Mutagenesisi274K → A: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with A-272 and A-273. 1 Publication1
Mutagenesisi282V → G: Does not inhibit calcium-dependent translocation to the cell membrane. Inhibits calcium-dependent translocation to the cell membrane; when associated with G-283 and G-284. 1 Publication1
Mutagenesisi282V → S: Inhibits calcium-dependent translocation to the cell membrane; when associated with S-283 and S-284. 1 Publication1
Mutagenesisi283V → G: Does not inhibit calcium-dependent translocation to the cell membrane. Inhibits calcium-dependent translocation to the cell membrane; when associated with G-282 and G-284. 1 Publication1
Mutagenesisi283V → S: Inhibits calcium-dependent translocation to the cell membrane; when associated with S-282 and S-284. 1 Publication1
Mutagenesisi284L → G: Inhibits partially calcium-dependent translocation to the cell membrane. Inhibits calcium-dependent translocation to the cell membrane; when associated with G-282 and G-283. 1 Publication1
Mutagenesisi284L → S: Inhibits calcium-dependent translocation to the cell membrane; when associated with S-282 and S-283. 1 Publication1
Mutagenesisi286Q → A: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with G-287 and G-288. 1 Publication1
Mutagenesisi287C → A: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with G-286 and G-288. 1 Publication1
Mutagenesisi288T → A: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with G-286 and G-287. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001448461 – 557Copine-6Add BLAST557

Proteomic databases

MaxQBiQ9Z140
PaxDbiQ9Z140
PeptideAtlasiQ9Z140
PRIDEiQ9Z140

PTM databases

iPTMnetiQ9Z140
PhosphoSitePlusiQ9Z140

Expressioni

Tissue specificityi

Expressed in the brain (PubMed:9645480). Expressed in pyramidal cells, granule cells, and neurons in the dentate gyrus of the hippocampus and in granule cells of the olfactory bulb (at protein level). Expressed in pyramidal cells of the CA1-CA3 regions, in granule cells of the dentate gyrus, in granule cells of the olfactory bulbs, in the mitral cell layer and in neurons of the cerebral cortex layer II, brainstem and spinal cord (PubMed:9886090). Not detected in glial cells (PubMed:9645480, PubMed:9886090).2 Publications

Inductioni

Up-regulated by long-term potentiation (PubMed:9645480). Up-regulated by kainate in an NMDA-type glutamate receptor-dependent manner (PubMed:9645480).1 Publication

Gene expression databases

BgeeiENSMUSG00000022212
CleanExiMM_CPNE6
ExpressionAtlasiQ9Z140 baseline and differential
GenevisibleiQ9Z140 MM

Interactioni

Subunit structurei

Interacts (via second C2 domain) with OS9 (via C-terminus); this interaction occurs in a calcium-dependent manner in vitro (PubMed:10403379). May interact with NECAB1 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi198861, 2 interactors
IntActiQ9Z140, 3 interactors
MINTiQ9Z140
STRINGi10090.ENSMUSP00000073847

Structurei

3D structure databases

ProteinModelPortaliQ9Z140
SMRiQ9Z140
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 111C2 1PROSITE-ProRule annotationAdd BLAST88
Domaini138 – 243C2 2PROSITE-ProRule annotationAdd BLAST106
Domaini306 – 526VWFAPROSITE-ProRule annotationAdd BLAST221

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni244 – 303Linker region2 PublicationsAdd BLAST60

Domaini

The C2 domain 1 binds phospholipids in a calcium-independent manner and is not necessary for calcium-mediated translocation and association to the plasma membrane (PubMed:9886090, PubMed:26175110). The C2 domain 2 binds phospholipids in a calcium-dependent manner and is necessary for calcium-mediated translocation and association to the plasma membrane (PubMed:9886090, PubMed:26175110). The linker region contributes to the calcium-dependent translocation and association to the plasma membrane (PubMed:21087455, PubMed:26175110). The VWFA domain is necessary for association with intracellular clathrin-coated vesicles in a calcium-dependent manner (PubMed:21087455).3 Publications

Sequence similaritiesi

Belongs to the copine family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1327 Eukaryota
ENOG410XPC8 LUCA
GeneTreeiENSGT00760000119085
HOGENOMiHOG000220898
HOVERGENiHBG066841
InParanoidiQ9Z140
OMAiDMSDPEM
OrthoDBiEOG091G05IT
PhylomeDBiQ9Z140
TreeFamiTF316419

Family and domain databases

CDDicd04047 C2B_Copine, 1 hit
cd01459 vWA_copine_like, 1 hit
Gene3Di2.60.40.150, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR037768 C2B_Copine
IPR010734 Copine
IPR002035 VWF_A
IPR036465 vWFA_dom_sf
PfamiView protein in Pfam
PF00168 C2, 2 hits
PF07002 Copine, 1 hit
SMARTiView protein in SMART
SM00239 C2, 2 hits
SM00327 VWA, 1 hit
SUPFAMiSSF53300 SSF53300, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50234 VWFA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z140-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDPEMGWVP EPPAMTLGAS RVELRVSCHG LLDRDTLTKP HPCVLLKLYS
60 70 80 90 100
DEQWVEVERT EVLRSCSSPV FSRVLAIEYF FEEKQPLQFH VFDAEDGATS
110 120 130 140 150
PSSDTFLGST ECTLGQIVSQ TKVTKPLLLK NGKTAGKSTI TIVAEEVSGT
160 170 180 190 200
NDYVQLTFRA HKLDNKDLFS KSDPFMEIYK TNGDQSDQLV WRTEVVKNNL
210 220 230 240 250
NPSWEPFRLS LHSLCSCDIH RPLKFLVYDY DSSGKHDFIG EFTSTFQEMQ
260 270 280 290 300
EGTANPGQEM QWDCINPKYR DKKKNYKSSG TVVLAQCTVE KVHTFLDYIM
310 320 330 340 350
GGCQISFTVA IDFTASNGDP RSSQSLHCLS PRQPNHYLQA LRTVGGICQD
360 370 380 390 400
YDSDKRFPAF GFGARIPPNF EVSHDFAINF DPENPECEEI SGVIASYRRC
410 420 430 440 450
LPQIQLYGPT NVAPIINRVA EPAQREQSTG QATKYSVLLV LTDGVVSDMA
460 470 480 490 500
ETRTAIVRAS RLPMSIIIVG VGNADFSDMR LLDGDDGPLR CPKGVPAARD
510 520 530 540 550
IVQFVPFRDF KDAAPSALAK CVLAEVPRQV VEYYASQGIS PGAPRPSTPA

MTPSPSP
Length:557
Mass (Da):61,781
Last modified:May 1, 1999 - v1
Checksum:i043D6C1487E2ECAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008893 mRNA Translation: BAA75898.1
BC050766 mRNA Translation: AAH50766.1
CCDSiCCDS27112.1
RefSeqiNP_001129529.1, NM_001136057.2
NP_034077.1, NM_009947.3
XP_006518569.1, XM_006518506.3
UniGeneiMm.5249

Genome annotation databases

EnsembliENSMUST00000074225; ENSMUSP00000073847; ENSMUSG00000022212
ENSMUST00000163767; ENSMUSP00000126493; ENSMUSG00000022212
ENSMUST00000171643; ENSMUSP00000128555; ENSMUSG00000022212
GeneIDi12891
KEGGimmu:12891
UCSCiuc007tyr.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCPNE6_MOUSE
AccessioniPrimary (citable) accession number: Q9Z140
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: March 28, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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