Q9Z137 (B3GA2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2
EC=2.4.1.135

Alternative name(s):
Beta-1,3-glucuronyltransferase 2
GlcAT-D
UDP-glucuronosyltransferase S
Short name=GlcAT-S
Short name=Glucuronosyltransferase S

Gene names

Name:	B3gat2
Synonyms:	Glcats

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins. Substrates include asialo-orosomucoid (ASOR), paragloboside (lacto-N-neotetraosylceramide), Gal-beta-1,4-GlcNAc-beta-1,3-Gal-beta-1,4-Glc-pyridylamine and Gal-beta-1,3-GlcNAc-beta-1,3-Gal-beta-1,4-Glc-pyridylamine.
Catalytic activity	UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer Potential.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Expressed in the cerebral cortex, cerebellum and whole brain.
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate biosynthetic process Inferred from mutant phenotype Ref.1. Source: RGD
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from direct assay Ref.2. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2		PRO_0000195174
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	24 – 324	301	Lumenal Potential
Sites
	Active site	274	1	Proton acceptor By similarity
	Metal binding	188	1	Manganese By similarity
Amino acid modifications
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9Z137 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 6283C29535B5D00E	FASTA	324	37,220
	10 20 30 40 50 60 MKSALCNRFF ILLPWILIVI IMLDVDPRRP APQLTSRPYF SPHTVGCGGS RVPLRRSSPG 70 80 90 100 110 120 RDAAEKRNES RPQLQPEPRL PTIYAITPTY SRPVQKAELT RLANTFRQVA QLHWILVEDR 130 140 150 160 170 180 ATRSELVSSF LARAGLPNTH LHVPTPRRYK RPWLPRATEQ RNAGLAWLRQ RHQHQSAQPG 190 200 210 220 230 240 VLFFADDDNT YSLELFQEMR TTRKVSVWPV GLVGGRRYER PLVKNGKVVG WYTGWREDRP 250 260 270 280 290 300 FAIDMAGFAV SLQVILSNPK AVFKRRGSQP GMQESDFLKQ ITTVDELEPK ANNCTKVLVW 310 320 HTRTEKVNLA NEPKYHMDTV NIEV

References

[1]	"Molecular cloning and expression of a second glucuronyltransferase involved in the biosynthesis of the HNK-1 carbohydrate epitope." Seiki T., Oka S., Terayama K., Imiya K., Kawasaki T. Biochem. Biophys. Res. Commun. 255:182-187(1999) [PubMed: 10082676] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Cloning and expression of a novel galactoside beta1,3-glucuronyltransferase involved in the biosynthesis of HNK-1 epitope." Shimoda Y., Tajima Y., Nagase T., Harii K., Osumi N., Sanai Y. J. Biol. Chem. 274:17115-17122(1999) [PubMed: 10358066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Embryonic brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB010441 mRNA. Translation: BAA75219.1. AF106624 mRNA. Translation: AAD29576.1.
IPI	IPI00208149.
PIR	JG0163.
RefSeq	NP_072131.1. NM_022609.1.
UniGene	Rn.42869.
3D structure databases
ProteinModelPortal	Q9Z137.
SMR	Q9Z137. Positions 80-324.
ModBase	Search...
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	64544.
KEGG	rno:64544.
Organism-specific databases
CTD	135152.
RGD	620903. B3gat2.
Phylogenomic databases
HOVERGEN	HBG050650.
Gene expression databases
Genevestigator	Q9Z137.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
KO	K10157.
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	613412.

Entry information

Entry name

B3GA2_RAT

Accession

Primary (citable) accession number: Q9Z137

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 1, 1999
Last modified:	November 16, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program