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Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2

Gene

B3gat2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins. Substrates include asialo-orosomucoid (ASOR), paragloboside (lacto-N-neotetraosylceramide), Gal-beta-1,4-GlcNAc-beta-1,3-Gal-beta-1,4-Glc-pyridylamine and Gal-beta-1,3-GlcNAc-beta-1,3-Gal-beta-1,4-Glc-pyridylamine.

Catalytic activityi

UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi188 – 1881ManganeseBy similarity
Active sitei274 – 2741Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

  • carbohydrate biosynthetic process Source: RGD
  • protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 (EC:2.4.1.135)
Alternative name(s):
Beta-1,3-glucuronyltransferase 2
GlcAT-D
UDP-glucuronosyltransferase S
Short name:
GlcAT-S
Short name:
Glucuronosyltransferase S
Gene namesi
Name:B3gat2
Synonyms:Glcats
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620903. B3gat2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 22CytoplasmicSequence analysis
Transmembranei3 – 2321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini24 – 324301LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2PRO_0000195174Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi68 – 681N-linked (GlcNAc...)Sequence analysis
Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9Z137.
PRIDEiQ9Z137.

Expressioni

Tissue specificityi

Expressed in the cerebral cortex, cerebellum and whole brain.

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063982.

Structurei

3D structure databases

ProteinModelPortaliQ9Z137.
SMRiQ9Z137. Positions 80-324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 43 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
HOVERGENiHBG050650.
InParanoidiQ9Z137.
KOiK10157.
PhylomeDBiQ9Z137.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z137-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSALCNRFF ILLPWILIVI IMLDVDPRRP APQLTSRPYF SPHTVGCGGS
60 70 80 90 100
RVPLRRSSPG RDAAEKRNES RPQLQPEPRL PTIYAITPTY SRPVQKAELT
110 120 130 140 150
RLANTFRQVA QLHWILVEDR ATRSELVSSF LARAGLPNTH LHVPTPRRYK
160 170 180 190 200
RPWLPRATEQ RNAGLAWLRQ RHQHQSAQPG VLFFADDDNT YSLELFQEMR
210 220 230 240 250
TTRKVSVWPV GLVGGRRYER PLVKNGKVVG WYTGWREDRP FAIDMAGFAV
260 270 280 290 300
SLQVILSNPK AVFKRRGSQP GMQESDFLKQ ITTVDELEPK ANNCTKVLVW
310 320
HTRTEKVNLA NEPKYHMDTV NIEV
Length:324
Mass (Da):37,220
Last modified:May 1, 1999 - v1
Checksum:i6283C29535B5D00E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010441 mRNA. Translation: BAA75219.1.
AF106624 mRNA. Translation: AAD29576.1.
PIRiJG0163.
RefSeqiNP_072131.1. NM_022609.2.
UniGeneiRn.42869.

Genome annotation databases

GeneIDi64544.
KEGGirno:64544.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010441 mRNA. Translation: BAA75219.1.
AF106624 mRNA. Translation: AAD29576.1.
PIRiJG0163.
RefSeqiNP_072131.1. NM_022609.2.
UniGeneiRn.42869.

3D structure databases

ProteinModelPortaliQ9Z137.
SMRiQ9Z137. Positions 80-324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063982.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Proteomic databases

PaxDbiQ9Z137.
PRIDEiQ9Z137.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64544.
KEGGirno:64544.

Organism-specific databases

CTDi135152.
RGDi620903. B3gat2.

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
HOVERGENiHBG050650.
InParanoidiQ9Z137.
KOiK10157.
PhylomeDBiQ9Z137.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

NextBioi613412.
PROiQ9Z137.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and expression of a second glucuronyltransferase involved in the biosynthesis of the HNK-1 carbohydrate epitope."
    Seiki T., Oka S., Terayama K., Imiya K., Kawasaki T.
    Biochem. Biophys. Res. Commun. 255:182-187(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Cloning and expression of a novel galactoside beta1,3-glucuronyltransferase involved in the biosynthesis of HNK-1 epitope."
    Shimoda Y., Tajima Y., Nagase T., Harii K., Osumi N., Sanai Y.
    J. Biol. Chem. 274:17115-17122(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Tissue: Embryonic brain.

Entry informationi

Entry nameiB3GA2_RAT
AccessioniPrimary (citable) accession number: Q9Z137
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 1999
Last modified: May 11, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.