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Protein

R-spondin-1

Gene

Rspo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.1 Publication

GO - Molecular functioni

  • G-protein coupled receptor binding Source: MGI
  • heparin binding Source: MGI
  • receptor binding Source: MGI

GO - Biological processi

  • canonical Wnt signaling pathway Source: MGI
  • male meiosis Source: MGI
  • positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  • positive regulation of non-canonical Wnt signaling pathway Source: MGI
  • positive regulation of protein phosphorylation Source: MGI
  • positive regulation of Wnt signaling pathway Source: UniProtKB
  • regulation of gene expression Source: MGI
  • regulation of male germ cell proliferation Source: MGI
  • regulation of receptor internalization Source: MGI
Complete GO annotation...

Keywords - Biological processi

Sensory transduction, Wnt signaling pathway

Keywords - Ligandi

Heparin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
R-spondin-1
Alternative name(s):
Cysteine-rich and single thrombospondin domain-containing protein 3
Short name:
Cristin-3
Short name:
mCristin-3
Roof plate-specific spondin-1
Gene namesi
Name:Rspo1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2183426. Rspo1.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 265245R-spondin-1PRO_0000234437Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi40 ↔ 47PROSITE-ProRule annotation
Disulfide bondi44 ↔ 53PROSITE-ProRule annotation
Disulfide bondi56 ↔ 75PROSITE-ProRule annotation
Disulfide bondi79 ↔ 94PROSITE-ProRule annotation
Disulfide bondi97 ↔ 105PROSITE-ProRule annotation
Disulfide bondi102 ↔ 111PROSITE-ProRule annotation
Disulfide bondi114 ↔ 125PROSITE-ProRule annotation
Disulfide bondi129 ↔ 142PROSITE-ProRule annotation
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence analysis
Disulfide bondi148 ↔ 190PROSITE-ProRule annotation
Disulfide bondi159 ↔ 166PROSITE-ProRule annotation
Disulfide bondi199 ↔ 206PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9Z132.
PRIDEiQ9Z132.

PTM databases

PhosphoSiteiQ9Z132.

Expressioni

Tissue specificityi

Expressed in the dorsal part of the neural tube on 10 and 12 dpc, especially in the boundary region between roof plate and neuroepithelium. This expression is enhanced in the rostral part. Also expressed in other tissues such as truncal region neighboring forelimbs and mesenchymal tissues around the nasal cavity.1 Publication

Developmental stagei

Transiently expressed in the central nervous system (CNS) during development. Predominantly expressed in the tailbud of embryos. Also detected in the primitive streak, dorsal neural tube, forebrain and migrating neural crests of embryos. Detected from day 9.5, in various neural and mesodermal derivatives, mainly along dorsal neural tube and diencephalon. Strongly expressed in limb buds, particularly in the morphogenetically active region such as the apical ectodermal ridge (AER). The developing skin showed expression in patches of the developing dermis at E12.5 and at lower levels at E14.5. By E18.5 expression is restricted to the dermal papilla of the developing hair, which persisted into adulthood. In the developing kidney, strong expression at E11.5 in the uninduced metanephric mesenchyme is observed. By E14.5 this expression is restricted to the condensing mesenchyme surrounding the ureter and the developing nephrons. Specific expression in the urogenital ridge as early as E10.5 in the coelomic epithelium. Sex-specific differences of expression began to appear starting at E12.5, with an increase in the somatic cells of the XX gonad.4 Publications

Gene expression databases

CleanExiMM_RSPO1.

Interactioni

Subunit structurei

Interacts with ZNRF3; promoting indirect interaction between ZNRF3 and LGR4 and membrane clearance of ZNRF3. Identified in a complex composed of RNF43, LGR5 and RSPO1 (By similarity). Interacts with the extracellular domain of FZD8 and LRP6. It however does not form a ternary complex with FZD8 and LRP6. Interacts with WNT1. Binds heparin. Interacts with LGR4, LGR5 and LGR6.By similarity2 Publications

GO - Molecular functioni

  • G-protein coupled receptor binding Source: MGI
  • receptor binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030687.

Structurei

3D structure databases

ProteinModelPortaliQ9Z132.
SMRiQ9Z132. Positions 40-132.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati34 – 8552FU 1Add
BLAST
Repeati91 – 13545FU 2Add
BLAST
Domaini147 – 20761TSP type-1PROSITE-ProRule annotationAdd
BLAST

Domaini

The FU repeats are required for activation and stabilization of beta-catenin.By similarity

Sequence similaritiesi

Belongs to the R-spondin family.Curated
Contains 2 FU (furin-like) repeats.Curated
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
COG1404. LUCA.
HOGENOMiHOG000290668.
HOVERGENiHBG082751.
InParanoidiQ9Z132.
KOiK19471.
PhylomeDBiQ9Z132.

Family and domain databases

InterProiIPR006212. Furin_repeat.
IPR009030. Growth_fac_rcpt_.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF15913. Furin-like_2. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
SMARTiSM00261. FU. 2 hits.
SM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS50092. TSP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z132-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLGLCVVAL VLSWTHIAVG SRGIKGKRQR RISAEGSQAC AKGCELCSEV
60 70 80 90 100
NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE
110 120 130 140 150
HCEACFSHNF CTKCQEALYL HKGRCYPACP EGSTAANSTM ECGSPAQCEM
160 170 180 190 200
SEWSPWGPCS KKRKLCGFRK GSEERTRRVL HAPGGDHTTC SDTKETRKCT
210 220 230 240 250
VRRTPCPEGQ KRRKGGQGRR ENANRHPARK NSKEPRSNSR RHKGQQQPQP
260
GTTGPLTSVG PTWAQ
Length:265
Mass (Da):29,332
Last modified:May 1, 1999 - v1
Checksum:iFFEB8964743F5963
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1171A → G in BAE21077 (PubMed:16141072).Curated
Sequence conflicti236 – 2361R → G in BAE21077 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016768 mRNA. Translation: BAA75640.1.
AK132277 mRNA. Translation: BAE21077.1.
CCDSiCCDS18633.1.
RefSeqiNP_619624.2. NM_138683.2.
UniGeneiMm.42202.

Genome annotation databases

GeneIDi192199.
KEGGimmu:192199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016768 mRNA. Translation: BAA75640.1.
AK132277 mRNA. Translation: BAE21077.1.
CCDSiCCDS18633.1.
RefSeqiNP_619624.2. NM_138683.2.
UniGeneiMm.42202.

3D structure databases

ProteinModelPortaliQ9Z132.
SMRiQ9Z132. Positions 40-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030687.

PTM databases

PhosphoSiteiQ9Z132.

Proteomic databases

PaxDbiQ9Z132.
PRIDEiQ9Z132.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192199.
KEGGimmu:192199.

Organism-specific databases

CTDi284654.
MGIiMGI:2183426. Rspo1.

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
COG1404. LUCA.
HOGENOMiHOG000290668.
HOVERGENiHBG082751.
InParanoidiQ9Z132.
KOiK19471.
PhylomeDBiQ9Z132.

Miscellaneous databases

PROiQ9Z132.
SOURCEiSearch...

Gene expression databases

CleanExiMM_RSPO1.

Family and domain databases

InterProiIPR006212. Furin_repeat.
IPR009030. Growth_fac_rcpt_.
IPR000884. TSP1_rpt.
[Graphical view]
PfamiPF15913. Furin-like_2. 1 hit.
PF00090. TSP_1. 1 hit.
[Graphical view]
SMARTiSM00261. FU. 2 hits.
SM00209. TSP1. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS50092. TSP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSPO1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z132
Secondary accession number(s): Q3V1S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.