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Q9Z129 (RECQ1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent DNA helicase Q1

EC=3.6.4.12
Alternative name(s):
DNA-dependent ATPase Q1
RecQ protein-like 1
Gene names
Name:Recql
Synonyms:Recql1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length648 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Isoform alphais a DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Isoform betamay have important roles in the meiotic process. Both isoforms exhibit a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Ref.1

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with EXO1 and MLH1 By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Isoform alphais expressed in all tissues examined. Isoform betais only expressed in spermatocytes, expression increases at pachytene (17 days old) and decreases after completion of meiosis II (7 weeks old). Ref.1

Sequence similarities

Belongs to the helicase family. RecQ subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha (identifier: Q9Z129-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: Q9Z129-2)

The sequence of this isoform differs from the canonical sequence as follows:
     622-648: NSQKSKSRLQPSGSKNAGAKKRKLDDA → SHAADTAANV

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 648648ATP-dependent DNA helicase Q1
PRO_0000205050

Regions

Domain100 – 275176Helicase ATP-binding
Domain299 – 451153Helicase C-terminal
Nucleotide binding113 – 1208ATP Potential
Motif219 – 2224DEVH box

Amino acid modifications

Modified residue5141N6-acetyllysine By similarity
Modified residue5221N6-acetyllysine By similarity
Modified residue6331Phosphoserine By similarity

Natural variations

Alternative sequence622 – 64827NSQKS…KLDDA → SHAADTAANV in isoform Beta.
VSP_005567

Experimental info

Sequence conflict2161A → V in BAA75085. Ref.1
Sequence conflict2161A → V in BAA75086. Ref.1
Sequence conflict5181A → T in BAA75085. Ref.1
Sequence conflict5181A → T in BAA75086. Ref.1
Sequence conflict5251A → P in BAA75085. Ref.1
Sequence conflict5251A → P in BAA75086. Ref.1
Sequence conflict6021L → P in BAA75085. Ref.1
Sequence conflict6021L → P in BAA75086. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: DF84B5E9097BF3F3

FASTA64872,484
        10         20         30         40         50         60 
MASVSALTEE LESVASELHA IDIQIQELTE RRQELLQRKS VLTGKIKQYL EDSSAEASSD 

        70         80         90        100        110        120 
LDTSPAAWNK EDFPWFGKVK DVLQNVFKLQ KFRPLQLETI NVTMARKDIF LVMPTGGGKS 

       130        140        150        160        170        180 
LCYQLPALCS DGFTLVICPL ISLMEDQLMV LKQLGISATM LNASSSKEHV KWVHAEMVNK 

       190        200        210        220        230        240 
NSQLKLIYVT PEKIAKSKMF MSRLEKAYEA GRLTGAAVDE VHCCSQWGHD FRPDYKALGI 

       250        260        270        280        290        300 
LKRQFPNASL MGLTATATNH VLKDVQKILC VGKCLTFTAS FNRPNLFYEV RQKPSSAEDF 

       310        320        330        340        350        360 
TEDIVKLING RYKGQSGIIY CFSQKDSEQI TISLQKLGIH AGTYHANMEP EDKTKVHTQW 

       370        380        390        400        410        420 
SANELQVVVA TVAFGMGIDK PDVRFVIHHS MSKSMENYYQ ESGRAGRDDS RADCILYYGF 

       430        440        450        460        470        480 
GDIFRISSMV VMENVGQQKL YEMVSYCQNV SKCRRVLIAQ HFDEVWNADA CNKMCDNCCK 

       490        500        510        520        530        540 
DVSFEKKNVT QHCRDLIKIL KQAEGLNEKL TPLKLIDAWM GKGAAKLRVA GVVAPALPRE 

       550        560        570        580        590        600 
DLERIVAHAL LQQYLKEDYS FTAYATISYL KVGPRACLLS NEAHAVTMQV KKSAQSSVRG 

       610        620        630        640 
ALSEARQVEQ VDSKGEEQSS GNSQKSKSRL QPSGSKNAGA KKRKLDDA 

« Hide

Isoform Beta [UniParc].

Checksum: 633BAB8A1CA86761
Show »

FASTA63170,540

References

« Hide 'large scale' references
[1]"Cloning of two isoforms of mouse DNA helicase Q1/RecQL cDNA; alpha form is expressed ubiquitously and beta form specifically in the testis."
Wang W.-S., Seki M., Yamaoka T., Seki T., Tada S., Katada T., Fujimoto H., Enomoto T.
Biochim. Biophys. Acta 1443:198-202(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
Tissue: Spermatocyte and Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA).
Strain: C57BL/6J.
Tissue: Bone and Spinal ganglion.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 186-196 AND 502-509, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB017104 mRNA. Translation: BAA75085.1.
AB017105 mRNA. Translation: BAA75086.1.
AK137589 mRNA. Translation: BAE23422.1.
AK164344 mRNA. Translation: BAE37751.1.
CH466572 Genomic DNA. Translation: EDL10638.1.
CCDSCCDS39695.1. [Q9Z129-1]
CCDS57465.1. [Q9Z129-2]
RefSeqNP_001191836.1. NM_001204907.1. [Q9Z129-2]
NP_075529.2. NM_023042.3. [Q9Z129-1]
XP_006506989.1. XM_006506926.1. [Q9Z129-1]
XP_006506990.1. XM_006506927.1. [Q9Z129-1]
UniGeneMm.27407.

3D structure databases

ProteinModelPortalQ9Z129.
SMRQ9Z129. Positions 64-593.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9Z129.

Proteomic databases

PaxDbQ9Z129.
PRIDEQ9Z129.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032370; ENSMUSP00000032370; ENSMUSG00000030243. [Q9Z129-2]
ENSMUST00000111803; ENSMUSP00000107434; ENSMUSG00000030243. [Q9Z129-1]
GeneID19691.
KEGGmmu:19691.
UCSCuc009epf.2. mouse. [Q9Z129-1]

Organism-specific databases

CTD5965.
MGIMGI:103021. Recql.

Phylogenomic databases

eggNOGCOG0514.
GeneTreeENSGT00550000074520.
HOGENOMHOG000044388.
HOVERGENHBG057654.
InParanoidQ3TPI5.
KOK10899.
OMAQKFRPLQ.
OrthoDBEOG7K0ZC0.
TreeFamTF323555.

Gene expression databases

ArrayExpressQ9Z129.
BgeeQ9Z129.
CleanExMM_RECQL.
GenevestigatorQ9Z129.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
TIGRFAMsTIGR00614. recQ_fam. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio297024.
PROQ9Z129.
SOURCESearch...

Entry information

Entry nameRECQ1_MOUSE
AccessionPrimary (citable) accession number: Q9Z129
Secondary accession number(s): Q3TPI5, Q9Z128
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot