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Q9Z127 (LAT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Large neutral amino acids transporter small subunit 1
Alternative name(s):
4F2 light chain
Short name=4F2 LC
Short name=4F2LC
L-type amino acid transporter 1
Solute carrier family 7 member 5
Gene names
Name:Slc7a5
Synonyms:Lat1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. Ref.1 Ref.5 Ref.6

Subunit structure

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Ref.1

Subcellular location

Cytoplasmcytosol. Apical cell membrane; Multi-pass membrane protein. Note: Located to the plasma membrane by SLC3A2/4F2hc. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells By similarity. Localized to the apical membrane of placental syncytiophoblastic cells By similarity. Ref.1

Tissue specificity

Predominantly expressed in the microvessels in the brain parenchyma of the central nervous system. Also detected in the subfornical organ, the subcommissural organ, ventromedial nucleus of the hypothalamus, subgranular zone of the dentate gyrus in hippocampus, ependymal layer of the lateral ventricles, and the olfactory bulb. Very strong expression also seen in testis, ovary, and placenta with weaker expression in spleen, skin, brain, thymus, stomach, lung, heart, kidney, small intestine, uterus and skeletal muscle. Ref.1 Ref.4 Ref.5 Ref.6

Developmental stage

Strong expression in the liver of 14 dpc embryo. In embryo of 18 dpc expressed strongly in brain, moderate expression in spleen and brain and weak expression in liver. Ref.1

Induction

Expression induced by concanavilin-A stimulation. Ref.1

Miscellaneous

Leucine uptake was inhibited by ileum, valine histidine and phenylalanine as well as by 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH) (a specific inhibitor of system L transport).

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=25 µM for leucine Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Large neutral amino acids transporter small subunit 1
PRO_0000054271

Regions

Transmembrane51 – 7121Helical; Potential
Transmembrane85 – 10521Helical; Potential
Transmembrane121 – 14121Helical; Potential
Transmembrane147 – 16721Helical; Potential
Transmembrane174 – 19421Helical; Potential
Transmembrane200 – 22021Helical; Potential
Transmembrane248 – 26821Helical; Potential
Transmembrane279 – 29921Helical; Potential
Transmembrane324 – 34421Helical; Potential
Transmembrane398 – 41821Helical; Potential
Transmembrane436 – 45621Helical; Potential
Transmembrane463 – 48321Helical; Potential

Amino acid modifications

Modified residue461Phosphothreonine By similarity
Glycosylation501N-linked (GlcNAc...) Potential
Glycosylation3451N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict81R → M in BAA90556. Ref.2
Sequence conflict161A → T in BAA75520. Ref.1
Sequence conflict161A → T in BAA90556. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9Z127 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 57045EC4DD9DE1A1

FASTA51255,872
        10         20         30         40         50         60 
MAVAGAKRRA VATPAAAAAE EERQAREKML EARRGDGADP EGEGVTLQRN ITLLNGVAII 

        70         80         90        100        110        120 
VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG ALCYAELGTT ISKSGGDYAY 

       130        140        150        160        170        180 
MLEVYGSLPA FLKLWIELLI IRPSSQYIVA LVFATYLLKP VFPTCPVPEE AAKLVACLCV 

       190        200        210        220        230        240 
LLLTAVNCYS VKAATRVQDA FAAAKLLALA LIILLGFIQM GKDMGQGDAS NLQQKLSFEG 

       250        260        270        280        290        300 
TNLDVGNIVL ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA 

       310        320        330        340        350        360 
YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT SSRLFFVGSR 

       370        380        390        400        410        420 
EGHLPSVLSM IHPQLLTPVP SLVFTCIMTL MYAFSRDIFS IINFFSFFNW LCVALAIIGM 

       430        440        450        460        470        480 
MWLRFKKPEL ERPIKVNLAL PVFFILACLF LIAVSFWKTP MECGIGFAII LSGLPVYFFG 

       490        500        510 
VWWKNKPKWI LQAIFSVTVL CQKLMQVVPQ ET 

« Hide

References

« Hide 'large scale' references
[1]"4F2 (CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer."
Nakamura E., Sato M., Yang H., Miyagawa F., Harasaki M., Tomita K., Matsuoka S., Noma A., Iwai K., Minato N.
J. Biol. Chem. 274:3009-3016(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, INHIBITION.
Strain: BALB/c.
[2]"Localization of expression of system L neutral amino acid transporter LAT1 in brain."
Kanai Y., Watanabe M., Endou H.
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Salivary gland.
[4]"LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C.
J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"The 4F2hc/LAT1 complex transports L-DOPA across the blood-brain barrier."
Kageyama T., Nakamura M., Matsuo A., Yamasaki Y., Takakura Y., Hashida M., Kanai Y., Naito M., Tsuruo T., Minato N., Shimohama S.
Brain Res. 879:115-121(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]"Distribution of the 4F2 light chain, LAT1, in the mouse brain."
Kageyama T., Imura T., Matsuo A., Minato N., Shimohama S.
NeuroReport 11:3663-3666(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB017189 mRNA. Translation: BAA75520.1.
AB023409 mRNA. Translation: BAA90556.1.
BC026131 mRNA. Translation: AAH26131.1.
CCDSCCDS22730.1.
RefSeqNP_035534.2. NM_011404.3.
UniGeneMm.27943.

3D structure databases

ProteinModelPortalQ9Z127.
SMRQ9Z127. Positions 59-395.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000041557.

PTM databases

PhosphoSiteQ9Z127.

Proteomic databases

MaxQBQ9Z127.
PaxDbQ9Z127.
PRIDEQ9Z127.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000045557; ENSMUSP00000041557; ENSMUSG00000040010.
GeneID20539.
KEGGmmu:20539.
UCSCuc009nsc.2. mouse.

Organism-specific databases

CTD8140.
MGIMGI:1298205. Slc7a5.

Phylogenomic databases

eggNOGCOG0531.
GeneTreeENSGT00730000110280.
HOGENOMHOG000098892.
HOVERGENHBG000476.
InParanoidQ8R0X8.
KOK13780.
OMAVFTCIMT.
OrthoDBEOG73BVCR.
TreeFamTF313355.

Gene expression databases

ArrayExpressQ9Z127.
BgeeQ9Z127.
GenevestigatorQ9Z127.

Family and domain databases

InterProIPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view]
PANTHERPTHR11785. PTHR11785. 1 hit.
PfamPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsTIGR00911. 2A0308. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSLC7A5. mouse.
NextBio298819.
PROQ9Z127.
SOURCESearch...

Entry information

Entry nameLAT1_MOUSE
AccessionPrimary (citable) accession number: Q9Z127
Secondary accession number(s): Q8R0X8, Q9JMI4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot