Large neutral amino acids transporter small subunit 1

Contribute

Send feedback

Read comments (?) or add your own

Q9Z127 (LAT1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Large neutral amino acids transporter small subunit 1

Alternative name(s):
4F2 light chain
Short name=4F2 LC
Short name=4F2LC
L-type amino acid transporter 1
Solute carrier family 7 member 5

Gene names

Name:	Slc7a5
Synonyms:	Lat1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. Ref.1 Ref.5 Ref.6
Subunit structure	Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Ref.1
Subcellular location	Cytoplasm › cytosol. Apical cell membrane; Multi-pass membrane protein. Note: Located to the plasma membrane by SLC3A2/4F2hc. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells By similarity. Localized to the apical membrane of placental syncytiophoblastic cells By similarity. Ref.1
Tissue specificity	Predominantly expressed in the microvessels in the brain parenchyma of the central nervous system. Also detected in the subfornical organ, the subcommissural organ, ventromedial nucleus of the hypothalamus, subgranular zone of the dentate gyrus in hippocampus, ependymal layer of the lateral ventricles, and the olfactory bulb. Very strong expression also seen in testis, ovary, and placenta with weaker expression in spleen, skin, brain, thymus, stomach, lung, heart, kidney, small intestine, uterus and skeletal muscle. Ref.1 Ref.4 Ref.5 Ref.6
Developmental stage	Strong expression in the liver of 14 dpc embryo. In embryo of 18 dpc expressed strongly in brain, moderate expression in spleen and brain and weak expression in liver. Ref.1
Induction	Expression induced by concanavilin-A stimulation. Ref.1
Miscellaneous	Leucine uptake was inhibited by ileum, valine histidine and phenylalanine as well as by 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH) (a specific inhibitor of system L transport).
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]
Biophysicochemical properties	Kinetic parameters: K_M=25 µM for leucine Ref.1

Ontologies

Keywords
Biological process	Amino-acid transport Differentiation Neurogenesis Transport
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-amino acid transmembrane transporter activity Inferred from direct assay PubMed 15659399. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 512

512

Large neutral amino acids transporter small subunit 1

PRO_0000054271

Regions

Transmembrane

51 – 71

Helical; Potential

Transmembrane

85 – 105

Helical; Potential

Transmembrane

121 – 141

Helical; Potential

Transmembrane

147 – 167

Helical; Potential

Transmembrane

174 – 194

Helical; Potential

Transmembrane

200 – 220

Helical; Potential

Transmembrane

248 – 268

Helical; Potential

Transmembrane

279 – 299

Helical; Potential

Transmembrane

324 – 344

Helical; Potential

Transmembrane

398 – 418

Helical; Potential

Transmembrane

436 – 456

Helical; Potential

Transmembrane

463 – 483

Helical; Potential

Amino acid modifications

Modified residue

512

Phosphothreonine By similarity

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

345

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

R → M in BAA90556. Ref.2

Sequence conflict

A → T in BAA75520. Ref.1

Sequence conflict

A → T in BAA90556. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q9Z127 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 57045EC4DD9DE1A1
Show »

FASTA

512

55,872

        10         20         30         40         50         60 
MAVAGAKRRA VATPAAAAAE EERQAREKML EARRGDGADP EGEGVTLQRN ITLLNGVAII 

        70         80         90        100        110        120 
VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG ALCYAELGTT ISKSGGDYAY 

       130        140        150        160        170        180 
MLEVYGSLPA FLKLWIELLI IRPSSQYIVA LVFATYLLKP VFPTCPVPEE AAKLVACLCV 

       190        200        210        220        230        240 
LLLTAVNCYS VKAATRVQDA FAAAKLLALA LIILLGFIQM GKDMGQGDAS NLQQKLSFEG 

       250        260        270        280        290        300 
TNLDVGNIVL ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA 

       310        320        330        340        350        360 
YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT SSRLFFVGSR 

       370        380        390        400        410        420 
EGHLPSVLSM IHPQLLTPVP SLVFTCIMTL MYAFSRDIFS IINFFSFFNW LCVALAIIGM 

       430        440        450        460        470        480 
MWLRFKKPEL ERPIKVNLAL PVFFILACLF LIAVSFWKTP MECGIGFAII LSGLPVYFFG 

       490        500        510 
VWWKNKPKWI LQAIFSVTVL CQKLMQVVPQ ET

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"4F2 (CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer." Nakamura E., Sato M., Yang H., Miyagawa F., Harasaki M., Tomita K., Matsuoka S., Noma A., Iwai K., Minato N. J. Biol. Chem. 274:3009-3016(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, INHIBITION. Strain: BALB/c.
[2]	"Localization of expression of system L neutral amino acid transporter LAT1 in brain." Kanai Y., Watanabe M., Endou H. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland.
[4]	"LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine." Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C. J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"The 4F2hc/LAT1 complex transports L-DOPA across the blood-brain barrier." Kageyama T., Nakamura M., Matsuo A., Yamasaki Y., Takakura Y., Hashida M., Kanai Y., Naito M., Tsuruo T., Minato N., Shimohama S. Brain Res. 879:115-121(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"Distribution of the 4F2 light chain, LAT1, in the mouse brain." Kageyama T., Imura T., Matsuo A., Minato N., Shimohama S. NeuroReport 11:3663-3666(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017189 mRNA. Translation: BAA75520.1. AB023409 mRNA. Translation: BAA90556.1. BC026131 mRNA. Translation: AAH26131.1.
IPI	IPI00129395.
RefSeq	NP_035534.2. NM_011404.3.
UniGene	Mm.27943.
3D structure databases
ProteinModelPortal	Q9Z127.
SMR	Q9Z127. Positions 59-395.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000041557.
PTM databases
PhosphoSite	Q9Z127.
Proteomic databases
PaxDb	Q9Z127.
PRIDE	Q9Z127.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000045557; ENSMUSP00000041557; ENSMUSG00000040010.
GeneID	20539.
KEGG	mmu:20539.
Organism-specific databases
CTD	8140.
MGI	MGI:1298205. Slc7a5.
Phylogenomic databases
eggNOG	COG0531.
GeneTree	ENSGT00690000101731.
HOGENOM	HOG000098892.
HOVERGEN	HBG000476.
InParanoid	Q8R0X8.
KO	K13780.
OMA	PIVTAVY.
OrthoDB	EOG4J1186.
Gene expression databases
ArrayExpress	Q9Z127.
Bgee	Q9Z127.
Genevestigator	Q9Z127.
GermOnline	ENSMUSG00000040010. Mus musculus.
Family and domain databases
InterPro	IPR002293. AA/rel_permease1. IPR004760. L_AA_transporter. [Graphical view]
PANTHER	PTHR11785. PTHR11785. 1 hit.
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00911. 2A0308. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	SLC7A5. mouse.
NextBio	298819.
SOURCE	Search...

Entry information

Entry name

LAT1_MOUSE

Accession

Primary (citable) accession number: Q9Z127
Secondary accession number(s): Q8R0X8, Q9JMI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents