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Protein

Large neutral amino acids transporter small subunit 1

Gene

Slc7a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.3 Publications

Kineticsi

  1. KM=25 µM for leucine1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Amino-acid transport, Differentiation, Neurogenesis, Transport

    Enzyme and pathway databases

    ReactomeiR-MMU-210991. Basigin interactions.
    R-MMU-352230. Amino acid transport across the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 1
    Alternative name(s):
    4F2 light chain
    Short name:
    4F2 LC
    Short name:
    4F2LC
    L-type amino acid transporter 1
    Solute carrier family 7 member 5
    Gene namesi
    Name:Slc7a5
    Synonyms:Lat1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:1298205. Slc7a5.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
    Transmembranei85 – 105HelicalSequence analysisAdd BLAST21
    Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
    Transmembranei147 – 167HelicalSequence analysisAdd BLAST21
    Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
    Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
    Transmembranei248 – 268HelicalSequence analysisAdd BLAST21
    Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
    Transmembranei324 – 344HelicalSequence analysisAdd BLAST21
    Transmembranei398 – 418HelicalSequence analysisAdd BLAST21
    Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
    Transmembranei463 – 483HelicalSequence analysisAdd BLAST21

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000542711 – 512Large neutral amino acids transporter small subunit 1Add BLAST512

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei46PhosphothreonineBy similarity1
    Glycosylationi50N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi345N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    EPDiQ9Z127.
    MaxQBiQ9Z127.
    PaxDbiQ9Z127.
    PRIDEiQ9Z127.

    PTM databases

    iPTMnetiQ9Z127.
    PhosphoSitePlusiQ9Z127.
    SwissPalmiQ9Z127.

    Expressioni

    Tissue specificityi

    Predominantly expressed in the microvessels in the brain parenchyma of the central nervous system. Also detected in the subfornical organ, the subcommissural organ, ventromedial nucleus of the hypothalamus, subgranular zone of the dentate gyrus in hippocampus, ependymal layer of the lateral ventricles, and the olfactory bulb. Very strong expression also seen in testis, ovary, and placenta with weaker expression in spleen, skin, brain, thymus, stomach, lung, heart, kidney, small intestine, uterus and skeletal muscle.4 Publications

    Developmental stagei

    Strong expression in the liver of 14 dpc embryo. In embryo of 18 dpc expressed strongly in brain, moderate expression in spleen and brain and weak expression in liver.1 Publication

    Inductioni

    Expression induced by concanavilin-A stimulation.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000040010.
    GenevisibleiQ9Z127. MM.

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.1 Publication

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000041557.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z127.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1287. Eukaryota.
    COG0531. LUCA.
    GeneTreeiENSGT00760000119037.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ9Z127.
    KOiK13780.
    OMAiMIWLRHR.
    OrthoDBiEOG091G07EM.
    TreeFamiTF313355.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9Z127-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAVAGAKRRA VATPAAAAAE EERQAREKML EARRGDGADP EGEGVTLQRN
    60 70 80 90 100
    ITLLNGVAII VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG
    110 120 130 140 150
    ALCYAELGTT ISKSGGDYAY MLEVYGSLPA FLKLWIELLI IRPSSQYIVA
    160 170 180 190 200
    LVFATYLLKP VFPTCPVPEE AAKLVACLCV LLLTAVNCYS VKAATRVQDA
    210 220 230 240 250
    FAAAKLLALA LIILLGFIQM GKDMGQGDAS NLQQKLSFEG TNLDVGNIVL
    260 270 280 290 300
    ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA
    310 320 330 340 350
    YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT
    360 370 380 390 400
    SSRLFFVGSR EGHLPSVLSM IHPQLLTPVP SLVFTCIMTL MYAFSRDIFS
    410 420 430 440 450
    IINFFSFFNW LCVALAIIGM MWLRFKKPEL ERPIKVNLAL PVFFILACLF
    460 470 480 490 500
    LIAVSFWKTP MECGIGFAII LSGLPVYFFG VWWKNKPKWI LQAIFSVTVL
    510
    CQKLMQVVPQ ET
    Length:512
    Mass (Da):55,872
    Last modified:July 27, 2011 - v2
    Checksum:i57045EC4DD9DE1A1
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti8R → M in BAA90556 (Ref. 2) Curated1
    Sequence conflicti16A → T in BAA75520 (PubMed:9915839).Curated1
    Sequence conflicti16A → T in BAA90556 (Ref. 2) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB017189 mRNA. Translation: BAA75520.1.
    AB023409 mRNA. Translation: BAA90556.1.
    BC026131 mRNA. Translation: AAH26131.1.
    CCDSiCCDS22730.1.
    RefSeqiNP_035534.2. NM_011404.3.
    UniGeneiMm.22374.
    Mm.27943.

    Genome annotation databases

    EnsembliENSMUST00000045557; ENSMUSP00000041557; ENSMUSG00000040010.
    GeneIDi20539.
    KEGGimmu:20539.
    UCSCiuc009nsc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB017189 mRNA. Translation: BAA75520.1.
    AB023409 mRNA. Translation: BAA90556.1.
    BC026131 mRNA. Translation: AAH26131.1.
    CCDSiCCDS22730.1.
    RefSeqiNP_035534.2. NM_011404.3.
    UniGeneiMm.22374.
    Mm.27943.

    3D structure databases

    ProteinModelPortaliQ9Z127.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000041557.

    PTM databases

    iPTMnetiQ9Z127.
    PhosphoSitePlusiQ9Z127.
    SwissPalmiQ9Z127.

    Proteomic databases

    EPDiQ9Z127.
    MaxQBiQ9Z127.
    PaxDbiQ9Z127.
    PRIDEiQ9Z127.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000045557; ENSMUSP00000041557; ENSMUSG00000040010.
    GeneIDi20539.
    KEGGimmu:20539.
    UCSCiuc009nsc.2. mouse.

    Organism-specific databases

    CTDi8140.
    MGIiMGI:1298205. Slc7a5.

    Phylogenomic databases

    eggNOGiKOG1287. Eukaryota.
    COG0531. LUCA.
    GeneTreeiENSGT00760000119037.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ9Z127.
    KOiK13780.
    OMAiMIWLRHR.
    OrthoDBiEOG091G07EM.
    TreeFamiTF313355.

    Enzyme and pathway databases

    ReactomeiR-MMU-210991. Basigin interactions.
    R-MMU-352230. Amino acid transport across the plasma membrane.

    Miscellaneous databases

    ChiTaRSiSlc7a5. mouse.
    PROiQ9Z127.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000040010.
    GenevisibleiQ9Z127. MM.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiLAT1_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z127
    Secondary accession number(s): Q8R0X8, Q9JMI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: July 27, 2011
    Last modified: November 2, 2016
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Leucine uptake was inhibited by ileum, valine histidine and phenylalanine as well as by 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH) (a specific inhibitor of system L transport).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.