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Q9Z127

- LAT1_MOUSE

UniProt

Q9Z127 - LAT1_MOUSE

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Protein

Large neutral amino acids transporter small subunit 1

Gene

Slc7a5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts.3 Publications

Kineticsi

  1. KM=25 µM for leucine1 Publication

GO - Molecular functioni

  1. L-amino acid transmembrane transporter activity Source: MGI

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. L-alpha-amino acid transmembrane transport Source: GOC
  3. L-amino acid transport Source: MGI
  4. nervous system development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Amino-acid transport, Differentiation, Neurogenesis, Transport

Enzyme and pathway databases

ReactomeiREACT_211508. Basigin interactions.
REACT_230460. Amino acid transport across the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Large neutral amino acids transporter small subunit 1
Alternative name(s):
4F2 light chain
Short name:
4F2 LC
Short name:
4F2LC
L-type amino acid transporter 1
Solute carrier family 7 member 5
Gene namesi
Name:Slc7a5
Synonyms:Lat1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1298205. Slc7a5.

Subcellular locationi

Cytoplasmcytosol 1 Publication. Apical cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Located to the plasma membrane by SLC3A2/4F2hc. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity). Localized to the apical membrane of placental syncytiophoblastic cells (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei51 – 7121HelicalSequence AnalysisAdd
BLAST
Transmembranei85 – 10521HelicalSequence AnalysisAdd
BLAST
Transmembranei121 – 14121HelicalSequence AnalysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence AnalysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence AnalysisAdd
BLAST
Transmembranei200 – 22021HelicalSequence AnalysisAdd
BLAST
Transmembranei248 – 26821HelicalSequence AnalysisAdd
BLAST
Transmembranei279 – 29921HelicalSequence AnalysisAdd
BLAST
Transmembranei324 – 34421HelicalSequence AnalysisAdd
BLAST
Transmembranei398 – 41821HelicalSequence AnalysisAdd
BLAST
Transmembranei436 – 45621HelicalSequence AnalysisAdd
BLAST
Transmembranei463 – 48321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
  4. nucleolus Source: Ensembl
  5. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Large neutral amino acids transporter small subunit 1PRO_0000054271Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461PhosphothreonineBy similarity
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9Z127.
PaxDbiQ9Z127.
PRIDEiQ9Z127.

PTM databases

PhosphoSiteiQ9Z127.

Expressioni

Tissue specificityi

Predominantly expressed in the microvessels in the brain parenchyma of the central nervous system. Also detected in the subfornical organ, the subcommissural organ, ventromedial nucleus of the hypothalamus, subgranular zone of the dentate gyrus in hippocampus, ependymal layer of the lateral ventricles, and the olfactory bulb. Very strong expression also seen in testis, ovary, and placenta with weaker expression in spleen, skin, brain, thymus, stomach, lung, heart, kidney, small intestine, uterus and skeletal muscle.4 Publications

Developmental stagei

Strong expression in the liver of 14 dpc embryo. In embryo of 18 dpc expressed strongly in brain, moderate expression in spleen and brain and weak expression in liver.1 Publication

Inductioni

Expression induced by concanavilin-A stimulation.1 Publication

Gene expression databases

BgeeiQ9Z127.
ExpressionAtlasiQ9Z127. differential.
GenevestigatoriQ9Z127.

Interactioni

Subunit structurei

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041557.

Structurei

3D structure databases

ProteinModelPortaliQ9Z127.
SMRiQ9Z127. Positions 59-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0531.
GeneTreeiENSGT00760000119037.
HOGENOMiHOG000098892.
HOVERGENiHBG000476.
InParanoidiQ9Z127.
KOiK13780.
OMAiVFTCIMT.
OrthoDBiEOG73BVCR.
TreeFamiTF313355.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00911. 2A0308. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z127-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVAGAKRRA VATPAAAAAE EERQAREKML EARRGDGADP EGEGVTLQRN
60 70 80 90 100
ITLLNGVAII VGTIIGSGIF VTPTGVLKEA GSPGLSLVVW AVCGVFSIVG
110 120 130 140 150
ALCYAELGTT ISKSGGDYAY MLEVYGSLPA FLKLWIELLI IRPSSQYIVA
160 170 180 190 200
LVFATYLLKP VFPTCPVPEE AAKLVACLCV LLLTAVNCYS VKAATRVQDA
210 220 230 240 250
FAAAKLLALA LIILLGFIQM GKDMGQGDAS NLQQKLSFEG TNLDVGNIVL
260 270 280 290 300
ALYSGLFAYG GWNYLNFVTE EMINPYRNLP LAIIISLPIV TLVYVLTNLA
310 320 330 340 350
YFTTLSTNQM LTSEAVAVDF GNYHLGVMSW IIPVFVGLSC FGSVNGSLFT
360 370 380 390 400
SSRLFFVGSR EGHLPSVLSM IHPQLLTPVP SLVFTCIMTL MYAFSRDIFS
410 420 430 440 450
IINFFSFFNW LCVALAIIGM MWLRFKKPEL ERPIKVNLAL PVFFILACLF
460 470 480 490 500
LIAVSFWKTP MECGIGFAII LSGLPVYFFG VWWKNKPKWI LQAIFSVTVL
510
CQKLMQVVPQ ET
Length:512
Mass (Da):55,872
Last modified:July 27, 2011 - v2
Checksum:i57045EC4DD9DE1A1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81R → M in BAA90556. 1 PublicationCurated
Sequence conflicti16 – 161A → T in BAA75520. (PubMed:9915839)Curated
Sequence conflicti16 – 161A → T in BAA90556. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017189 mRNA. Translation: BAA75520.1.
AB023409 mRNA. Translation: BAA90556.1.
BC026131 mRNA. Translation: AAH26131.1.
CCDSiCCDS22730.1.
RefSeqiNP_035534.2. NM_011404.3.
UniGeneiMm.27943.

Genome annotation databases

EnsembliENSMUST00000045557; ENSMUSP00000041557; ENSMUSG00000040010.
GeneIDi20539.
KEGGimmu:20539.
UCSCiuc009nsc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017189 mRNA. Translation: BAA75520.1 .
AB023409 mRNA. Translation: BAA90556.1 .
BC026131 mRNA. Translation: AAH26131.1 .
CCDSi CCDS22730.1.
RefSeqi NP_035534.2. NM_011404.3.
UniGenei Mm.27943.

3D structure databases

ProteinModelPortali Q9Z127.
SMRi Q9Z127. Positions 59-395.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000041557.

PTM databases

PhosphoSitei Q9Z127.

Proteomic databases

MaxQBi Q9Z127.
PaxDbi Q9Z127.
PRIDEi Q9Z127.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000045557 ; ENSMUSP00000041557 ; ENSMUSG00000040010 .
GeneIDi 20539.
KEGGi mmu:20539.
UCSCi uc009nsc.2. mouse.

Organism-specific databases

CTDi 8140.
MGIi MGI:1298205. Slc7a5.

Phylogenomic databases

eggNOGi COG0531.
GeneTreei ENSGT00760000119037.
HOGENOMi HOG000098892.
HOVERGENi HBG000476.
InParanoidi Q9Z127.
KOi K13780.
OMAi VFTCIMT.
OrthoDBi EOG73BVCR.
TreeFami TF313355.

Enzyme and pathway databases

Reactomei REACT_211508. Basigin interactions.
REACT_230460. Amino acid transport across the plasma membrane.

Miscellaneous databases

ChiTaRSi Slc7a5. mouse.
NextBioi 298819.
PROi Q9Z127.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z127.
ExpressionAtlasi Q9Z127. differential.
Genevestigatori Q9Z127.

Family and domain databases

InterProi IPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view ]
PANTHERi PTHR11785. PTHR11785. 1 hit.
Pfami PF13520. AA_permease_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006060. AA_transporter. 1 hit.
TIGRFAMsi TIGR00911. 2A0308. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "4F2 (CD98) heavy chain is associated covalently with an amino acid transporter and controls intracellular trafficking and membrane topology of 4F2 heterodimer."
    Nakamura E., Sato M., Yang H., Miyagawa F., Harasaki M., Tomita K., Matsuoka S., Noma A., Iwai K., Minato N.
    J. Biol. Chem. 274:3009-3016(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION, INHIBITION.
    Strain: BALB/c.
  2. "Localization of expression of system L neutral amino acid transporter LAT1 in brain."
    Kanai Y., Watanabe M., Endou H.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  4. "LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
    Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C.
    J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "Distribution of the 4F2 light chain, LAT1, in the mouse brain."
    Kageyama T., Imura T., Matsuo A., Minato N., Shimohama S.
    NeuroReport 11:3663-3666(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiLAT1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z127
Secondary accession number(s): Q8R0X8, Q9JMI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Leucine uptake was inhibited by ileum, valine histidine and phenylalanine as well as by 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH) (a specific inhibitor of system L transport).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3