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Protein

Platelet factor 4

Gene

Pf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-140875. Common Pathway of Fibrin Clot Formation.
R-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-380108. Chemokine receptors bind chemokines.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet factor 4
Short name:
PF-4
Alternative name(s):
C-X-C motif chemokine 4
Gene namesi
Name:Pf4
Synonyms:Cxcl4, Scyb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1888711. Pf4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929By similarityAdd
BLAST
Chaini30 – 10576Platelet factor 4PRO_0000005069Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi31 – 311O-linked (GalNAc...)By similarity
Disulfide bondi44 ↔ 71By similarity
Disulfide bondi46 ↔ 87By similarity
Modified residuei61 – 611PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9Z126.
PaxDbiQ9Z126.
PeptideAtlasiQ9Z126.
PRIDEiQ9Z126.

PTM databases

iPTMnetiQ9Z126.

Expressioni

Gene expression databases

BgeeiQ9Z126.
CleanExiMM_PF4.
ExpressionAtlasiQ9Z126. baseline and differential.
GenevisibleiQ9Z126. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-61569N.
IntActiQ9Z126. 1 interaction.
MINTiMINT-4107892.
STRINGi10090.ENSMUSP00000031320.

Structurei

3D structure databases

ProteinModelPortaliQ9Z126.
SMRiQ9Z126. Positions 40-105.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni96 – 1027Heparin-bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J314. Eukaryota.
ENOG4111556. LUCA.
GeneTreeiENSGT00530000062901.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiQ9Z126.
KOiK05407.
OMAiCAVPQLI.
OrthoDBiEOG7B5WZS.
PhylomeDBiQ9Z126.
TreeFamiTF333433.

Family and domain databases

InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
IPR027222. PF4.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PTHR10179:SF43. PTHR10179:SF43. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z126-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVAAVFRGL RPSPELLLLG LLFLPAVVAV TSAGPEESDG DLSCVCVKTI
60 70 80 90 100
SSGIHLKHIT SLEVIKAGRH CAVPQLIATL KNGRKICLDR QAPLYKKVIK

KILES
Length:105
Mass (Da):11,243
Last modified:May 1, 1999 - v1
Checksum:i94F0190EFACF1A6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017491 mRNA. Translation: BAA75660.1.
AF349465 Genomic DNA. Translation: AAK30163.1.
CCDSiCCDS19416.1.
RefSeqiNP_064316.1. NM_019932.4.
UniGeneiMm.332490.

Genome annotation databases

EnsembliENSMUST00000031320; ENSMUSP00000031320; ENSMUSG00000029373.
GeneIDi56744.
KEGGimmu:56744.
UCSCiuc008ybi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017491 mRNA. Translation: BAA75660.1.
AF349465 Genomic DNA. Translation: AAK30163.1.
CCDSiCCDS19416.1.
RefSeqiNP_064316.1. NM_019932.4.
UniGeneiMm.332490.

3D structure databases

ProteinModelPortaliQ9Z126.
SMRiQ9Z126. Positions 40-105.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61569N.
IntActiQ9Z126. 1 interaction.
MINTiMINT-4107892.
STRINGi10090.ENSMUSP00000031320.

PTM databases

iPTMnetiQ9Z126.

Proteomic databases

MaxQBiQ9Z126.
PaxDbiQ9Z126.
PeptideAtlasiQ9Z126.
PRIDEiQ9Z126.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031320; ENSMUSP00000031320; ENSMUSG00000029373.
GeneIDi56744.
KEGGimmu:56744.
UCSCiuc008ybi.2. mouse.

Organism-specific databases

CTDi5196.
MGIiMGI:1888711. Pf4.

Phylogenomic databases

eggNOGiENOG410J314. Eukaryota.
ENOG4111556. LUCA.
GeneTreeiENSGT00530000062901.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiQ9Z126.
KOiK05407.
OMAiCAVPQLI.
OrthoDBiEOG7B5WZS.
PhylomeDBiQ9Z126.
TreeFamiTF333433.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-140875. Common Pathway of Fibrin Clot Formation.
R-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-380108. Chemokine receptors bind chemokines.
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiQ9Z126.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z126.
CleanExiMM_PF4.
ExpressionAtlasiQ9Z126. baseline and differential.
GenevisibleiQ9Z126. MM.

Family and domain databases

InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
IPR027222. PF4.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PTHR10179:SF43. PTHR10179:SF43. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Significantly elevated expression of PF4 (platelet factor 4) and eotaxin in the NOA mouse, a model for atopic dermatitis."
    Watanabe O., Natori K., Tamari M., Shiomoto Y., Kubo S., Nakamura Y.
    J. Hum. Genet. 44:173-176(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Spleen.
  2. "Localization of distal regulatory domains in the megakaryocyte-specific platelet basic protein/platelet factor 4 gene locus."
    Zhang C., Thornton M.A., Kowalska M.A., Sachis B.S., Feldman M., Poncz M., McKenzie S.E., Reilly M.P.
    Blood 98:610-617(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiPLF4_MOUSE
AccessioniPrimary (citable) accession number: Q9Z126
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: May 1, 1999
Last modified: July 6, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.