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Q9Z120

- TRMB_MOUSE

UniProt

Q9Z120 - TRMB_MOUSE

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

Mettl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei154 – 1541S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei157 – 1571UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
  2. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA (guanine-N7)-methylation Source: GOC
  2. tRNA modification Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Methyltransferase-like protein 1UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:Mettl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1339986. Mettl1.

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleolus Source: Ensembl
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268tRNA (guanine-N(7)-)-methyltransferasePRO_0000171432Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211Phosphoserine; by PKB and RPS6KA3UniRule annotation

Post-translational modificationi

Phosphorylation at Ser-21 inactivates its catalytic activity but does not affect the interaction with WDR4.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z120.
PaxDbiQ9Z120.
PRIDEiQ9Z120.

PTM databases

PhosphoSiteiQ9Z120.

Expressioni

Gene expression databases

BgeeiQ9Z120.
CleanExiMM_METTL1.
ExpressionAtlasiQ9Z120. baseline and differential.
GenevestigatoriQ9Z120.

Interactioni

Subunit structurei

Forms a complex with WDR4.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ9Z120.
SMRiQ9Z120. Positions 31-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni101 – 1022S-adenosyl-L-methionine bindingUniRule annotation
Regioni134 – 1352S-adenosyl-L-methionine bindingUniRule annotation
Regioni232 – 2343S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
GeneTreeiENSGT00390000017840.
HOGENOMiHOG000260965.
HOVERGENiHBG044569.
InParanoidiQ9Z120.
KOiK03439.
OMAiRAHSNPI.
PhylomeDBiQ9Z120.
TreeFamiTF314083.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z120-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMAGAEAPQP QKRYYRQRAH SNPMADHTLR YPVKPEEMDW SELYPEFFAP
60 70 80 90 100
LIQNKSHDDP KDEKEKHSGA QVEFADIGCG YGGLLVALSP LFPDTLILGL
110 120 130 140 150
EIRVKVSDYV QDRIRALRAA PGGGFQNIAC LRSNAMKHLP NFFRKGQLAK
160 170 180 190 200
MFFLFPDPHF KRTKHKWRII SPTLLAEYAY VLRVGGLVYT VTDVPELHEW
210 220 230 240 250
MCTHFEEHPL FERVPLEELS EDPIVEHLGS STEEGKKVLR NGGKNFPAVF
260
RRIQDPLLQA VTPNPTLP
Length:268
Mass (Da):30,603
Last modified:May 1, 1999 - v1
Checksum:i1DAE1EEAA4A07627
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521I → N in AAH12649. (PubMed:15489334)Curated
Sequence conflicti56 – 561S → N in AAH12649. (PubMed:15489334)Curated
Sequence conflicti213 – 2131R → C in AAH12649. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB023619 mRNA. Translation: BAA75230.1.
AK160914 mRNA. Translation: BAE36088.1.
CH466578 Genomic DNA. Translation: EDL24462.1.
BC012649 mRNA. Translation: AAH12649.1.
Y18644 mRNA. Translation: CAA77240.1.
CCDSiCCDS24223.1.
RefSeqiNP_034922.1. NM_010792.1.
UniGeneiMm.251593.

Genome annotation databases

EnsembliENSMUST00000006915; ENSMUSP00000006915; ENSMUSG00000006732.
GeneIDi17299.
KEGGimmu:17299.
UCSCiuc007hhq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB023619 mRNA. Translation: BAA75230.1 .
AK160914 mRNA. Translation: BAE36088.1 .
CH466578 Genomic DNA. Translation: EDL24462.1 .
BC012649 mRNA. Translation: AAH12649.1 .
Y18644 mRNA. Translation: CAA77240.1 .
CCDSi CCDS24223.1.
RefSeqi NP_034922.1. NM_010792.1.
UniGenei Mm.251593.

3D structure databases

ProteinModelPortali Q9Z120.
SMRi Q9Z120. Positions 31-259.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q9Z120.

Proteomic databases

MaxQBi Q9Z120.
PaxDbi Q9Z120.
PRIDEi Q9Z120.

Protocols and materials databases

DNASUi 17299.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000006915 ; ENSMUSP00000006915 ; ENSMUSG00000006732 .
GeneIDi 17299.
KEGGi mmu:17299.
UCSCi uc007hhq.1. mouse.

Organism-specific databases

CTDi 4234.
MGIi MGI:1339986. Mettl1.

Phylogenomic databases

eggNOGi COG0220.
GeneTreei ENSGT00390000017840.
HOGENOMi HOG000260965.
HOVERGENi HBG044569.
InParanoidi Q9Z120.
KOi K03439.
OMAi RAHSNPI.
PhylomeDBi Q9Z120.
TreeFami TF314083.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Miscellaneous databases

NextBioi 291830.
PROi Q9Z120.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z120.
CleanExi MM_METTL1.
ExpressionAtlasi Q9Z120. baseline and differential.
Genevestigatori Q9Z120.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Methyltransferase related protein."
    Tamura M., Nashimoto M., Kaspar R.L.
    Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Extraembryonic tissue and Placenta.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Molecular analysis of METTL1, a novel human methyltransferase-like gene with a high degree of phylogenetic conservation."
    Bahr A., Hankeln T., Fiedler T., Hegemann J., Schmidt E.R.
    Genomics 57:424-428(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-268.
    Tissue: Brain.

Entry informationi

Entry nameiTRMB_MOUSE
AccessioniPrimary (citable) accession number: Q9Z120
Secondary accession number(s): Q3TU83, Q921G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: May 1, 1999
Last modified: October 29, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3