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Q9Z120

- TRMB_MOUSE

UniProt

Q9Z120 - TRMB_MOUSE

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

Mettl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei78 – 781S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei154 – 1541S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei157 – 1571UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
    2. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA modification Source: UniProtKB

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    Methyltransferase-like protein 1UniRule annotation
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:Mettl1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1339986. Mettl1.

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 268268tRNA (guanine-N(7)-)-methyltransferasePRO_0000171432Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei21 – 211Phosphoserine; by PKB and RPS6KA3UniRule annotation

    Post-translational modificationi

    Phosphorylation at Ser-21 inactivates its catalytic activity but does not affect the interaction with WDR4.UniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9Z120.
    PaxDbiQ9Z120.
    PRIDEiQ9Z120.

    PTM databases

    PhosphoSiteiQ9Z120.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Z120.
    BgeeiQ9Z120.
    CleanExiMM_METTL1.
    GenevestigatoriQ9Z120.

    Interactioni

    Subunit structurei

    Forms a complex with WDR4.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z120.
    SMRiQ9Z120. Positions 31-259.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni101 – 1022S-adenosyl-L-methionine bindingUniRule annotation
    Regioni134 – 1352S-adenosyl-L-methionine bindingUniRule annotation
    Regioni232 – 2343S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    GeneTreeiENSGT00390000017840.
    HOGENOMiHOG000260965.
    HOVERGENiHBG044569.
    InParanoidiQ3TU83.
    KOiK03439.
    OMAiRAHSNPI.
    PhylomeDBiQ9Z120.
    TreeFamiTF314083.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z120-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMAGAEAPQP QKRYYRQRAH SNPMADHTLR YPVKPEEMDW SELYPEFFAP    50
    LIQNKSHDDP KDEKEKHSGA QVEFADIGCG YGGLLVALSP LFPDTLILGL 100
    EIRVKVSDYV QDRIRALRAA PGGGFQNIAC LRSNAMKHLP NFFRKGQLAK 150
    MFFLFPDPHF KRTKHKWRII SPTLLAEYAY VLRVGGLVYT VTDVPELHEW 200
    MCTHFEEHPL FERVPLEELS EDPIVEHLGS STEEGKKVLR NGGKNFPAVF 250
    RRIQDPLLQA VTPNPTLP 268
    Length:268
    Mass (Da):30,603
    Last modified:May 1, 1999 - v1
    Checksum:i1DAE1EEAA4A07627
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 521I → N in AAH12649. (PubMed:15489334)Curated
    Sequence conflicti56 – 561S → N in AAH12649. (PubMed:15489334)Curated
    Sequence conflicti213 – 2131R → C in AAH12649. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB023619 mRNA. Translation: BAA75230.1.
    AK160914 mRNA. Translation: BAE36088.1.
    CH466578 Genomic DNA. Translation: EDL24462.1.
    BC012649 mRNA. Translation: AAH12649.1.
    Y18644 mRNA. Translation: CAA77240.1.
    CCDSiCCDS24223.1.
    RefSeqiNP_034922.1. NM_010792.1.
    UniGeneiMm.251593.

    Genome annotation databases

    EnsembliENSMUST00000006915; ENSMUSP00000006915; ENSMUSG00000006732.
    GeneIDi17299.
    KEGGimmu:17299.
    UCSCiuc007hhq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB023619 mRNA. Translation: BAA75230.1 .
    AK160914 mRNA. Translation: BAE36088.1 .
    CH466578 Genomic DNA. Translation: EDL24462.1 .
    BC012649 mRNA. Translation: AAH12649.1 .
    Y18644 mRNA. Translation: CAA77240.1 .
    CCDSi CCDS24223.1.
    RefSeqi NP_034922.1. NM_010792.1.
    UniGenei Mm.251593.

    3D structure databases

    ProteinModelPortali Q9Z120.
    SMRi Q9Z120. Positions 31-259.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9Z120.

    Proteomic databases

    MaxQBi Q9Z120.
    PaxDbi Q9Z120.
    PRIDEi Q9Z120.

    Protocols and materials databases

    DNASUi 17299.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006915 ; ENSMUSP00000006915 ; ENSMUSG00000006732 .
    GeneIDi 17299.
    KEGGi mmu:17299.
    UCSCi uc007hhq.1. mouse.

    Organism-specific databases

    CTDi 4234.
    MGIi MGI:1339986. Mettl1.

    Phylogenomic databases

    eggNOGi COG0220.
    GeneTreei ENSGT00390000017840.
    HOGENOMi HOG000260965.
    HOVERGENi HBG044569.
    InParanoidi Q3TU83.
    KOi K03439.
    OMAi RAHSNPI.
    PhylomeDBi Q9Z120.
    TreeFami TF314083.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Miscellaneous databases

    NextBioi 291830.
    PROi Q9Z120.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z120.
    Bgeei Q9Z120.
    CleanExi MM_METTL1.
    Genevestigatori Q9Z120.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Methyltransferase related protein."
      Tamura M., Nashimoto M., Kaspar R.L.
      Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Extraembryonic tissue and Placenta.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Molecular analysis of METTL1, a novel human methyltransferase-like gene with a high degree of phylogenetic conservation."
      Bahr A., Hankeln T., Fiedler T., Hegemann J., Schmidt E.R.
      Genomics 57:424-428(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-268.
      Tissue: Brain.

    Entry informationi

    Entry nameiTRMB_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z120
    Secondary accession number(s): Q3TU83, Q921G5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2003
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3