Reviewed,
UniProtKB/Swiss-Prot Q9Z110 (P5CS_MOUSE)
Last modified
February 9, 2010.
Version 95.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Delta-1-pyrroline-5-carboxylate synthetase Short name=P5CS Alternative name(s): Aldehyde dehydrogenase family 18 member A1 Including the following 2 domains: 1- Recommended name: Glutamate 5-kinase Short name=GK EC=2.7.2.11 Alternative name(s): Gamma-glutamyl kinase 2- Recommended name: Gamma-glutamyl phosphate reductase Short name=GPR EC=1.2.1.41 Alternative name(s): Glutamate-5-semialdehyde dehydrogenase Glutamyl-gamma-semialdehyde dehydrogenase | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 795 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | ATP + L-glutamate = ADP + L-glutamate 5-phosphate. L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. |
| Enzyme regulation | The short isoform is inhibited by L-ornithine with a Ki of approximately 0.25 mm. The long isoform is insensitive to ornithine inhibition. Thus, the two amino acid insert in the long isoform abolishes feedback inhibition of P5CS activity by L-ornithine. Ref.1 |
| Pathway | |
| Subcellular location | |
| Sequence similarities | In the N-terminal section; belongs to the glutamate 5-kinase family. In the C-terminal section; belongs to the gamma-glutamyl phosphate reductase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Proline biosynthesis |
| Cellular component | Membrane Mitochondrion Mitochondrion inner membrane |
| Coding sequence diversity | Alternative splicing |
| Ligand | ATP-binding NADP Nucleotide-binding |
| Molecular function | Kinase Oxidoreductase Transferase |
| Technical term | Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Ref.1Inferred from direct assay. Source: MGI glutamate-5-semialdehyde dehydrogenase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Long (identifier: Q9Z110-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: Q9Z110-2) The sequence of this isoform differs from the canonical sequence as follows: 239-240: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 795 | 795 | Delta-1-pyrroline-5-carboxylate synthetase | PRO_0000109770 | |||||
Regions | |||||||||
| Region | 1 – 361 | 361 | Glutamate 5-kinase | ||||||
| Region | 362 – 795 | 434 | Gamma-glutamyl phosphate reductase | ||||||
Natural variations | |||||||||
| Alternative sequence | 239 – 240 | 2 | Missing in isoform Short. | VSP_005216 | |||||
Sequences
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References
| [1] | "Molecular enzymology of mammalian delta1-pyrroline-5-carboxylate synthase. Alternative splice donor utilization generates isoforms with different sensitivity to ornithine inhibition." Hu C.A., Lin W.-W., Obie C., Valle D. J. Biol. Chem. 274:6754-6762(1999) [PubMed: 10037775] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, ENZYME REGULATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF056573 mRNA. Translation: AAD17517.1. AF056574 mRNA. Translation: AAD17518.1. |
| IPI | IPI00129350. IPI00221398. |
| UniGene | Mm.233117 |
3D structure databases | |
| SMR | Q9Z110. Positions 69-355, 336-764, 362-793. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9Z110. |
PTM databases | |
| PhosphoSite | Q9Z110. |
Proteomic databases | |
| PRIDE | Q9Z110. |
Genome annotation databases | |
| Ensembl | ENSMUST00000025979; ENSMUSP00000025979; ENSMUSG00000025007; Mus musculus. [Genome view] |
| UCSC | uc008hkw.1. mouse. uc008hkx.1. mouse. |
Organism-specific databases | |
| MGI | MGI:1888908. Aldh18a1. |
Phylogenomic databases | |
| HOGENOM | HBG318080. |
| HOVERGEN | Q9Z110. |
| InParanoid | Q9Z110. |
| PhylomeDB | Q9Z110. |
Enzyme and pathway databases | |
| BRENDA | 1.2.1.41. 244. 2.7.2.11. 244. |
Gene expression databases | |
| ArrayExpress | Q9Z110. |
| Bgee | Q9Z110. |
| CleanEx | MM_ALDH18A1. |
| Genevestigator | Q9Z110. |
| GermOnline | ENSMUSG00000025007. Mus musculus. |
Family and domain databases | |
| InterPro | IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR001048. Asp/Glu/Uridylate_kinase. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR001057. Glu_5kinase. IPR019797. Glutamate_5-kinase_CS. IPR005766. P5_carboxy_syn. IPR005715. ProB. [Graphical view] |
| Gene3D | G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit. |
| Pfam | PF00696. AA_kinase. 1 hit. PF00171. Aldedh. 1 hit. [Graphical view] |
| PIRSF | PIRSF036429. P5C_syn. 1 hit. |
| PRINTS | PR00474. GLU5KINASE. |
| TIGRFAMs | TIGR01092. P5CS. 1 hit. TIGR00407. proA. 1 hit. TIGR01027. proB. 1 hit. |
| PROSITE | PS00902. GLUTAMATE_5_KINASE. 1 hit. PS01223. PROA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 312680. |
| SOURCE | Search... |
Entry information
| Entry name | P5CS_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q9Z110 Secondary accession number(s): Q9R1P6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


