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Q9Z104 (HM20B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related

Short name=SMARCE1-related protein
Alternative name(s):
BRCA2-associated factor 35
HMG box-containing protein 20B
Structural DNA-binding protein BRAF35
Gene names
Name:Hmg20b
Synonyms:Braf35, Hmgx2, Smarce1r
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters By similarity.

Subunit structure

Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with the BRCA2 tumor suppressor protein By similarity.

Subcellular location

Nucleus By similarity. Chromosome By similarity. Note: Localized to condensed chromosomes in mitosis in conjunction with BRCA2 By similarity.

Tissue specificity

Widely expressed in adult tissues, particularly kidney, skin, testis and uterus. Highly expressed in embryonic tissues with high mitotic index, such as the proliferating ventricular zones of the fore-, mid- and hindbrain.

Sequence similarities

Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
   Biological processCell cycle
Transcription
Transcription regulation
   Cellular componentChromosome
Nucleus
   DomainCoiled coil
   LigandDNA-binding
   Molecular functionChromatin regulator
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

chromatin organization

Non-traceable author statement Ref.2. Source: UniProtKB

chromatin remodeling

Traceable author statement Ref.1. Source: ProtInc

negative regulation of protein sumoylation

Inferred from direct assay PubMed 22570500. Source: MGI

positive regulation of neuron differentiation

Inferred from genetic interaction PubMed 22570500. Source: MGI

regulation of transcription, DNA-templated

Non-traceable author statement Ref.2. Source: UniProtKB

skeletal muscle cell differentiation

Inferred from mutant phenotype PubMed 22147266. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentBRCA2-BRAF35 complex

Inferred from sequence alignment PubMed 11207365. Source: MGI

chromosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Non-traceable author statement Ref.2. Source: UniProtKB

   Molecular_functionDNA binding

Traceable author statement Ref.1. Source: ProtInc

four-way junction DNA binding

Inferred from sequence alignment PubMed 11207365. Source: MGI

protein binding

Inferred from physical interaction PubMed 15102471. Source: IntAct

protein heterodimerization activity

Inferred from physical interaction PubMed 22570500. Source: MGI

sequence-specific DNA binding transcription factor activity

Non-traceable author statement Ref.2. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Traf1P394283EBI-646920,EBI-520123

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related
PRO_0000048576

Regions

DNA binding70 – 13869HMG box
Coiled coil190 – 25768 Potential

Secondary structure

......... 317
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9Z104 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 6C4D45D2EB1D403B

FASTA31735,870
        10         20         30         40         50         60 
MSHGPRQPGA ATAPAGGKTP GQHGAFVVAV KQERSEGSRA GEKGPQEEEP VKKRGWPKGK 

        70         80         90        100        110        120 
KRKKILPNGP KAPVTGYVRF LNERREQIRT RHPDLPFPEI TKMLGAEWSK LQPAEKQRYL 

       130        140        150        160        170        180 
DEAEKEKQQY LKELWAYQQS EAYKVCTEKI QENKIKKEDS SSGLMNTLLN GHKGVDCDGF 

       190        200        210        220        230        240 
STFDVPIFTE EFLDQNKARE AELRRLRKMN VAFEEQNAVL QRHTQSMSSA RERLEQELAL 

       250        260        270        280        290        300 
EERRTLALQQ QLQAVRQALT ASFASLPVPG TGETPTLGTL DFYMARLHGA IERDPAQHER 

       310 
LIARVKEILA RVASEHL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, chromosomal location, and expression analysis of murine Smarce1-related, a new member of the high-mobility 365 group gene family."
Wattler F., Wattler S., Kelly M., Skinner H.B., Nehls M.
Genomics 60:172-178(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"HMG20A and HMG20B map to human chromosomes 15q24 and 19p13.3 and constitute a distinct class of HMG-box genes with ubiquitous expression."
Sumoy L., Carim-Todd L., Escarceller M., Nadal M., Gratacos M., Pujana M.A., Estivill X., Peral B.
Cytogenet. Cell Genet. 88:62-67(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor.
[4]"Solution structure of the HMG domain of mouse HMG domain protein HMGX2."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2005) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 69-148.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF067430 Genomic DNA. Translation: AAD15897.1.
AF146224 mRNA. Translation: AAF66708.1.
AL355734 mRNA. Translation: CAB90813.1.
AL355735 mRNA. Translation: CAB90814.1.
BC011334 mRNA. Translation: AAH11334.1.
CCDSCCDS24055.1.
RefSeqNP_001156637.1. NM_001163165.1.
NP_001156638.1. NM_001163166.1.
NP_034570.1. NM_010440.3.
UniGeneMm.38474.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2CRJNMR-A70-148[»]
ProteinModelPortalQ9Z104.
SMRQ9Z104. Positions 68-148.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200335. 1 interaction.
IntActQ9Z104. 1 interaction.

PTM databases

PhosphoSiteQ9Z104.

Proteomic databases

PRIDEQ9Z104.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020454; ENSMUSP00000020454; ENSMUSG00000020232.
ENSMUST00000105323; ENSMUSP00000100960; ENSMUSG00000020232.
ENSMUST00000105324; ENSMUSP00000100961; ENSMUSG00000020232.
ENSMUST00000167481; ENSMUSP00000128807; ENSMUSG00000020232.
GeneID15353.
KEGGmmu:15353.
UCSCuc007ghj.2. mouse.

Organism-specific databases

CTD10362.
MGIMGI:1341190. Hmg20b.

Phylogenomic databases

eggNOGNOG241877.
GeneTreeENSGT00730000110938.
HOGENOMHOG000247029.
HOVERGENHBG059870.
InParanoidQ9Z104.
OMAMKELREY.
OrthoDBEOG72G18C.
PhylomeDBQ9Z104.
TreeFamTF106440.

Gene expression databases

ArrayExpressQ9Z104.
BgeeQ9Z104.
CleanExMM_HMG20B.
GenevestigatorQ9Z104.

Family and domain databases

Gene3D1.10.30.10. 1 hit.
InterProIPR009071. HMG_box_dom.
[Graphical view]
PfamPF00505. HMG_box. 1 hit.
[Graphical view]
SMARTSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMSSF47095. SSF47095. 1 hit.
PROSITEPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHMG20B. mouse.
EvolutionaryTraceQ9Z104.
NextBio287960.
PROQ9Z104.
SOURCESearch...

Entry information

Entry nameHM20B_MOUSE
AccessionPrimary (citable) accession number: Q9Z104
Secondary accession number(s): Q9NSF7, Q9NSF8
Entry history
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot