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Protein

Partitioning defective 6 homolog alpha

Gene

Pard6a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.1 Publication

GO - Molecular functioni

  • GTP-dependent protein binding Source: UniProtKB
  • protein kinase C binding Source: MGI
  • Rho GTPase binding Source: UniProtKB

GO - Biological processi

  • cell-cell junction maintenance Source: UniProtKB
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • establishment of cell polarity Source: UniProtKB
  • negative regulation of protein phosphorylation Source: MGI
  • regulation of cellular localization Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

ReactomeiR-MMU-2173791. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
R-MMU-420029. Tight junction interactions.
R-MMU-4608870. Asymmetric localization of PCP proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning defective 6 homolog alpha
Short name:
PAR-6
Short name:
PAR-6 alpha
Short name:
PAR-6A
Gene namesi
Name:Pard6a
Synonyms:Par6a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1927223. Pard6a.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • apical part of cell Source: MGI
  • axonal growth cone Source: Ensembl
  • bicellular tight junction Source: UniProtKB
  • cell cortex Source: MGI
  • cell junction Source: MGI
  • cytoplasm Source: MGI
  • neuronal cell body Source: Ensembl
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Partitioning defective 6 homolog alphaPRO_0000112514Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei278 – 2781PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated by the TGF-beta receptor.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z101.
PaxDbiQ9Z101.
PRIDEiQ9Z101.

PTM databases

iPTMnetiQ9Z101.
PhosphoSiteiQ9Z101.

Expressioni

Gene expression databases

BgeeiQ9Z101.
ExpressionAtlasiQ9Z101. baseline and differential.
GenevisibleiQ9Z101. MM.

Interactioni

Subunit structurei

Interacts with ECT2 ('Thr-359' phosphorylated form) and PRKCI. Interacts with MPP5 and CRB3 (By similarity). Interacts with PARD3. Interacts with GTP-bound forms of CDC42, ARHQ/TC10 and RAC1. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI. Interacts with MAP2K5. Interacts with TGFBR1; involved in TGF-beta induced epithelial to mesenchymal transition.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DVL2O146416EBI-81732,EBI-740850From a different organism.

GO - Molecular functioni

  • GTP-dependent protein binding Source: UniProtKB
  • protein kinase C binding Source: MGI
  • Rho GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi208027. 9 interactions.
DIPiDIP-32553N.
IntActiQ9Z101. 8 interactions.
MINTiMINT-1340547.
STRINGi10090.ENSMUSP00000090886.

Structurei

3D structure databases

ProteinModelPortaliQ9Z101.
SMRiQ9Z101. Positions 14-95, 126-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 9581PB1PROSITE-ProRule annotationAdd
BLAST
Domaini133 – 15018Pseudo-CRIBAdd
BLAST
Domaini157 – 25094PDZPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 116116Interaction with PRKCI and PRKCZBy similarityAdd
BLAST
Regioni126 – 253128Interaction with PARD3 and CDC42By similarityAdd
BLAST

Domaini

The PB1 domain mediates interactions with MAP2K5.1 Publication
The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.By similarity
The PDZ domain mediates the interaction with CRB3.By similarity

Sequence similaritiesi

Belongs to the PAR6 family.Curated
Contains 1 PB1 domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
Contains 1 pseudo-CRIB domain.Curated

Phylogenomic databases

eggNOGiKOG3606. Eukaryota.
ENOG410XRJ3. LUCA.
GeneTreeiENSGT00390000005255.
HOGENOMiHOG000231716.
HOVERGENiHBG053509.
InParanoidiQ9Z101.
KOiK06093.
OMAiCWDLHPG.
PhylomeDBiQ9Z101.
TreeFamiTF312899.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001478. PDZ.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z101-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARPQRTPAR SPDSIVEVKS KFDAEFRRFA LPRTSVRGFQ EFSRLLCVVH
60 70 80 90 100
QIPGLDVLLG YTDAHGDLLP LTNDDSLHRA LASGPPPLRL LVQKRAEGDS
110 120 130 140 150
SGLAFASNSL QRRKKGLLLR PVAPLRTRPP LLISLPQDFR QVSSVIDVDL
160 170 180 190 200
LPETHRRVRL HKHGSDRPLG FYIRDGMSVR VAPQGLERVP GIFISRLVRG
210 220 230 240 250
GLAESTGLLA VSDEILEVNG IEVAGKTLDQ VTDMMVANSH NLIVTVKPAN
260 270 280 290 300
QRNNVVRGAS GRLTGPSSVG PGPTDPDSDD DSSDLVIENR HPPCSNGLSQ
310 320 330 340
GPLCWDLQPG CLLPGAGSSL PSLDSREQAN SGWGNGMRGD VSGFSL
Length:346
Mass (Da):37,333
Last modified:August 30, 2005 - v2
Checksum:i8F150B83B7E1F36A
GO
Isoform 2 (identifier: Q9Z101-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-96: Missing.

Show »
Length:345
Mass (Da):37,262
Checksum:i228E1D7FECE4521E
GO
Isoform 3 (identifier: Q9Z101-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     66-95: Missing.

Note: No experimental confirmation available.
Show »
Length:316
Mass (Da):34,088
Checksum:i5FA6A10190CA1CAD
GO

Sequence cautioni

The sequence AAD15928.1 differs from that shown. Reason: Frameshift at positions 108 and 121. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831P → L in AAH61114 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei66 – 9530Missing in isoform 3. 1 PublicationVSP_015463Add
BLAST
Alternative sequencei96 – 961Missing in isoform 2. 1 PublicationVSP_015462

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070970 mRNA. Translation: AAD15928.1. Frameshift.
BC049593 mRNA. Translation: AAH49593.1.
BC061114 mRNA. Translation: AAH61114.1.
CCDSiCCDS22609.1. [Q9Z101-1]
RefSeqiNP_001040900.1. NM_001047435.2. [Q9Z101-2]
NP_062669.2. NM_019695.3. [Q9Z101-1]
UniGeneiMm.472753.

Genome annotation databases

EnsembliENSMUST00000093195; ENSMUSP00000090886; ENSMUSG00000005699. [Q9Z101-1]
GeneIDi56513.
KEGGimmu:56513.
UCSCiuc009ndv.2. mouse. [Q9Z101-1]
uc009ndx.2. mouse. [Q9Z101-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070970 mRNA. Translation: AAD15928.1. Frameshift.
BC049593 mRNA. Translation: AAH49593.1.
BC061114 mRNA. Translation: AAH61114.1.
CCDSiCCDS22609.1. [Q9Z101-1]
RefSeqiNP_001040900.1. NM_001047435.2. [Q9Z101-2]
NP_062669.2. NM_019695.3. [Q9Z101-1]
UniGeneiMm.472753.

3D structure databases

ProteinModelPortaliQ9Z101.
SMRiQ9Z101. Positions 14-95, 126-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208027. 9 interactions.
DIPiDIP-32553N.
IntActiQ9Z101. 8 interactions.
MINTiMINT-1340547.
STRINGi10090.ENSMUSP00000090886.

PTM databases

iPTMnetiQ9Z101.
PhosphoSiteiQ9Z101.

Proteomic databases

MaxQBiQ9Z101.
PaxDbiQ9Z101.
PRIDEiQ9Z101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000093195; ENSMUSP00000090886; ENSMUSG00000005699. [Q9Z101-1]
GeneIDi56513.
KEGGimmu:56513.
UCSCiuc009ndv.2. mouse. [Q9Z101-1]
uc009ndx.2. mouse. [Q9Z101-3]

Organism-specific databases

CTDi50855.
MGIiMGI:1927223. Pard6a.

Phylogenomic databases

eggNOGiKOG3606. Eukaryota.
ENOG410XRJ3. LUCA.
GeneTreeiENSGT00390000005255.
HOGENOMiHOG000231716.
HOVERGENiHBG053509.
InParanoidiQ9Z101.
KOiK06093.
OMAiCWDLHPG.
PhylomeDBiQ9Z101.
TreeFamiTF312899.

Enzyme and pathway databases

ReactomeiR-MMU-2173791. TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition).
R-MMU-420029. Tight junction interactions.
R-MMU-4608870. Asymmetric localization of PCP proteins.

Miscellaneous databases

PROiQ9Z101.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z101.
ExpressionAtlasiQ9Z101. baseline and differential.
GenevisibleiQ9Z101. MM.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR000270. PB1_dom.
IPR001478. PDZ.
[Graphical view]
PfamiPF00564. PB1. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00666. PB1. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51745. PB1. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PAR-6 is a conserved PDZ domain-containing protein that colocalizes with PAR-3 in Caenorhabditis elegans embryos."
    Hung T.-J., Kemphues K.J.
    Development 126:127-135(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Brain and Testis.
  3. "A mammalian PAR-3-PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity."
    Lin D., Edwards A.S., Fawcett J.P., Mbamalu G., Scott J.D., Pawson T.
    Nat. Cell Biol. 2:540-547(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PARD3; PRKCZ; RAC1 AND CDC42, SUBUNIT OF A COMPLEX CONTAINING CDC42 AND PARD3.
  4. "Interaction codes within the family of mammalian Phox and Bem1p domain-containing proteins."
    Lamark T., Perander M., Outzen H., Kristiansen K., Oevervatn A., Michaelsen E., Bjoerkoey G., Johansen T.
    J. Biol. Chem. 278:34568-34581(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAP2K5, DOMAIN.
  5. "A polarity complex of mPar-6 and atypical PKC binds, phosphorylates and regulates mammalian Lgl."
    Plant P.J., Fawcett J.P., Lin D.C., Holdorf A.D., Binns K., Kulkarni S., Pawson T.
    Nat. Cell Biol. 5:301-308(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LLGL1 AND PRKCI.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  7. "Regulation of the polarity protein Par6 by TGFbeta receptors controls epithelial cell plasticity."
    Ozdamar B., Bose R., Barrios-Rodiles M., Wang H.R., Zhang Y., Wrana J.L.
    Science 307:1603-1609(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN EPITHELIAL TO MESENCHYMAL TRANSITION, INTERACTION WITH TGFBR1, PHOSPHORYLATION.

Entry informationi

Entry nameiPAR6A_MOUSE
AccessioniPrimary (citable) accession number: Q9Z101
Secondary accession number(s): Q5RL03, Q6P8R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: August 30, 2005
Last modified: June 8, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.