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Protein

Partitioning defective 6 homolog alpha

Gene

Pard6a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:15761148). Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization (By similarity).By similarity1 Publication

GO - Molecular functioni

  • GTP-dependent protein binding Source: UniProtKB
  • protein kinase C binding Source: MGI
  • Rho GTPase binding Source: UniProtKB

GO - Biological processi

  • cell-cell junction maintenance Source: UniProtKB
  • cell division Source: UniProtKB-KW
  • centrosome cycle Source: UniProtKB
  • establishment of cell polarity Source: UniProtKB
  • negative regulation of protein phosphorylation Source: MGI
  • positive regulation of protein localization to centrosome Source: UniProtKB

Keywordsi

Biological processCell cycle, Cell division

Enzyme and pathway databases

ReactomeiR-MMU-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition)
R-MMU-420029 Tight junction interactions
R-MMU-4608870 Asymmetric localization of PCP proteins

Names & Taxonomyi

Protein namesi
Recommended name:
Partitioning defective 6 homolog alpha
Short name:
PAR-6
Short name:
PAR-6 alpha
Short name:
PAR-6A
Gene namesi
Name:Pard6a
Synonyms:Par6a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1927223 Pard6a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125141 – 346Partitioning defective 6 homolog alphaAdd BLAST346

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei278PhosphoserineCombined sources1
Modified residuei345PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by the TGF-beta receptor.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Z101
PRIDEiQ9Z101

PTM databases

iPTMnetiQ9Z101
PhosphoSitePlusiQ9Z101

Expressioni

Gene expression databases

BgeeiENSMUSG00000005699
ExpressionAtlasiQ9Z101 baseline and differential
GenevisibleiQ9Z101 MM

Interactioni

Subunit structurei

Interacts with ECT2 ('Thr-359' phosphorylated form) and PRKCI. Interacts with MPP5 and CRB3 (By similarity). Interacts with PARD3. Interacts with GTP-bound forms of CDC42, ARHQ/TC10 and RAC1. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI. Interacts with MAP2K5. Interacts with TGFBR1; involved in TGF-beta induced epithelial to mesenchymal transition. Interacts with DCTN1 and PCM1 (By similarity).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DVL2O146416EBI-81732,EBI-740850From Homo sapiens.

GO - Molecular functioni

  • GTP-dependent protein binding Source: UniProtKB
  • protein kinase C binding Source: MGI
  • Rho GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi208027, 9 interactors
CORUMiQ9Z101
DIPiDIP-32553N
IntActiQ9Z101, 10 interactors
MINTiQ9Z101
STRINGi10090.ENSMUSP00000090886

Structurei

3D structure databases

ProteinModelPortaliQ9Z101
SMRiQ9Z101
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 95PB1PROSITE-ProRule annotationAdd BLAST81
Domaini133 – 150Pseudo-CRIBAdd BLAST18
Domaini157 – 250PDZPROSITE-ProRule annotationAdd BLAST94

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 116Interaction with PRKCI and PRKCZBy similarityAdd BLAST116
Regioni126 – 253Interaction with PARD3 and CDC42By similarityAdd BLAST128

Domaini

The PB1 domain mediates interactions with MAP2K5.1 Publication
The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.By similarity
The PDZ domain mediates the interaction with CRB3.By similarity

Sequence similaritiesi

Belongs to the PAR6 family.Curated

Phylogenomic databases

eggNOGiKOG3606 Eukaryota
ENOG410XRJ3 LUCA
GeneTreeiENSGT00390000005255
HOGENOMiHOG000231716
HOVERGENiHBG053509
InParanoidiQ9Z101
KOiK06093
OMAiEQASSGW
OrthoDBiEOG091G0FFH
PhylomeDBiQ9Z101
TreeFamiTF312899

Family and domain databases

CDDicd06403 PB1_Par6, 1 hit
InterProiView protein in InterPro
IPR034872 PAR-6_a
IPR000270 PB1_dom
IPR034868 PB1_Par6
IPR001478 PDZ
IPR036034 PDZ_sf
PANTHERiPTHR14102:SF9 PTHR14102:SF9, 1 hit
PfamiView protein in Pfam
PF00564 PB1, 1 hit
PF00595 PDZ, 1 hit
SMARTiView protein in SMART
SM00666 PB1, 1 hit
SM00228 PDZ, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS51745 PB1, 1 hit
PS50106 PDZ, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z101-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARPQRTPAR SPDSIVEVKS KFDAEFRRFA LPRTSVRGFQ EFSRLLCVVH
60 70 80 90 100
QIPGLDVLLG YTDAHGDLLP LTNDDSLHRA LASGPPPLRL LVQKRAEGDS
110 120 130 140 150
SGLAFASNSL QRRKKGLLLR PVAPLRTRPP LLISLPQDFR QVSSVIDVDL
160 170 180 190 200
LPETHRRVRL HKHGSDRPLG FYIRDGMSVR VAPQGLERVP GIFISRLVRG
210 220 230 240 250
GLAESTGLLA VSDEILEVNG IEVAGKTLDQ VTDMMVANSH NLIVTVKPAN
260 270 280 290 300
QRNNVVRGAS GRLTGPSSVG PGPTDPDSDD DSSDLVIENR HPPCSNGLSQ
310 320 330 340
GPLCWDLQPG CLLPGAGSSL PSLDSREQAN SGWGNGMRGD VSGFSL
Length:346
Mass (Da):37,333
Last modified:August 30, 2005 - v2
Checksum:i8F150B83B7E1F36A
GO
Isoform 2 (identifier: Q9Z101-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     96-96: Missing.

Show »
Length:345
Mass (Da):37,262
Checksum:i228E1D7FECE4521E
GO
Isoform 3 (identifier: Q9Z101-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     66-95: Missing.

Note: No experimental confirmation available.
Show »
Length:316
Mass (Da):34,088
Checksum:i5FA6A10190CA1CAD
GO

Sequence cautioni

The sequence AAD15928 differs from that shown. Reason: Frameshift at positions 108 and 121.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti183P → L in AAH61114 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01546366 – 95Missing in isoform 3. 1 PublicationAdd BLAST30
Alternative sequenceiVSP_01546296Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070970 mRNA Translation: AAD15928.1 Frameshift.
BC049593 mRNA Translation: AAH49593.1
BC061114 mRNA Translation: AAH61114.1
CCDSiCCDS22609.1 [Q9Z101-1]
CCDS85600.1 [Q9Z101-3]
CCDS85601.1 [Q9Z101-2]
RefSeqiNP_001040900.1, NM_001047435.2 [Q9Z101-2]
NP_062669.2, NM_019695.3 [Q9Z101-1]
UniGeneiMm.472753

Genome annotation databases

EnsembliENSMUST00000093195; ENSMUSP00000090886; ENSMUSG00000005699 [Q9Z101-1]
ENSMUST00000211888; ENSMUSP00000148821; ENSMUSG00000005699 [Q9Z101-3]
ENSMUST00000212430; ENSMUSP00000148603; ENSMUSG00000005699 [Q9Z101-2]
GeneIDi56513
KEGGimmu:56513
UCSCiuc009ndv.2 mouse [Q9Z101-1]
uc009ndx.2 mouse [Q9Z101-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPAR6A_MOUSE
AccessioniPrimary (citable) accession number: Q9Z101
Secondary accession number(s): Q5RL03, Q6P8R2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: August 30, 2005
Last modified: April 25, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health