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Protein

Probable carboxypeptidase X1

Gene

Cpxm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in cell-cell interactions. No carboxypeptidase activity was found yet.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi349 – 3491ZincBy similarity
Metal bindingi352 – 3521ZincBy similarity
Metal bindingi487 – 4871ZincBy similarity
Active sitei580 – 5801NucleophileBy similarity

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell adhesion Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM14.952.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable carboxypeptidase X1 (EC:3.4.17.-)
Alternative name(s):
Metallocarboxypeptidase CPX-1
Gene namesi
Name:Cpxm1
Synonyms:Cpx1, Cpxm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1934569. Cpxm1.

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 722702Probable carboxypeptidase X1PRO_0000004408Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi105 ↔ 263PROSITE-ProRule annotation
Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi461 – 4611N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ9Z100.

PTM databases

PhosphoSiteiQ9Z100.

Expressioni

Tissue specificityi

Strongly expressed in testis and spleen. Moderatly expressed in salivary gland, brain, heart, lung, and kidney. Extremely low expression in liver and muscle. No expression in eye, adrenal, and white adipose tissues.1 Publication

Developmental stagei

First expressed at 13.5 dpc, in the meninges, nasal mesenchyme, primordial cartilage and skeletal structures.1 Publication

Gene expression databases

BgeeiQ9Z100.
ExpressionAtlasiQ9Z100. baseline and differential.
GenevestigatoriQ9Z100.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028897.

Structurei

3D structure databases

ProteinModelPortaliQ9Z100.
SMRiQ9Z100. Positions 107-263, 284-667.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini103 – 263161F5/8 type CPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi62 – 654Poly-Lys
Compositional biasi358 – 3625Poly-Leu

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated
Contains 1 F5/8 type C domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG322453.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ9Z100.
KOiK08638.
OMAiRYDWVTS.
OrthoDBiEOG7B8S32.
TreeFamiTF315592.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR008979. Galactose-bd-like.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00231. FA58C. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
SSF49785. SSF49785. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
PS01285. FA58C_1. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z100-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWGLLLAVTA FAPSVGLGLG APSASVPGLA PGSTLAPHSS VAQPSTKANE
60 70 80 90 100
TSERHVRLRV IKKKKIVVKK RKKLRHPGPL GTARPVVPTH PAKTLTLPEK
110 120 130 140 150
QEPGCPPLGL ESLRVSDSQL EASSSQSFGL GAHRGRLNIQ SGLEDGDLYD
160 170 180 190 200
GAWCAEQQDT EPWLQVDAKN PVRFAGIVTQ GRNSVWRYDW VTSFKVQFSN
210 220 230 240 250
DSQTWWKSRN STGMDIVFPA NSDAETPVLN LLPEPQVARF IRLLPQTWFQ
260 270 280 290 300
GGAPCLRAEI LACPVSDPND LFPEAHTLGS SNSLDFRHHN YKAMRKLMKQ
310 320 330 340 350
VNEQCPNITR IYSIGKSHQG LKLYVMEMSD HPGEHELGEP EVRYVAGMHG
360 370 380 390 400
NEALGRELLL LLMQFLCHEF LRGDPRVTRL LTETRIHLLP SMNPDGYETA
410 420 430 440 450
YHRGSELVGW AEGRWTHQGI DLNHNFADLN TQLWYAEDDG LVPDTVPNHH
460 470 480 490 500
LPLPTYYTLP NATVAPETWA VIKWMKRIPF VLSANLHGGE LVVSYPFDMT
510 520 530 540 550
RTPWAARELT PTPDDAVFRW LSTVYAGTNR AMQDTDRRPC HSQDFSLHGN
560 570 580 590 600
VINGADWHTV PGSMNDFSYL HTNCFEVTVE LSCDKFPHEK ELPQEWENNK
610 620 630 640 650
DALLTYLEQV RMGITGVVRD KDTELGIADA VIAVEGINHD VTTAWGGDYW
660 670 680 690 700
RLLTPGDYVV TASAEGYHTV RQHCQVTFEE GPVPCNFLLT KTPKERLREL
710 720
LATRGKLPPD LRRKLERLRG QK
Length:722
Mass (Da):80,907
Last modified:July 27, 2011 - v2
Checksum:iCC365C119420A9D4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti253 – 2531A → V in AAD15985. (PubMed:10073577)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077738 mRNA. Translation: AAD15985.1.
BX890605 Genomic DNA. Translation: CAM20117.1.
CH466519 Genomic DNA. Translation: EDL28262.1.
BC003713 mRNA. Translation: AAH03713.1.
CCDSiCCDS16740.1.
RefSeqiNP_062670.2. NM_019696.2.
UniGeneiMm.112701.

Genome annotation databases

EnsembliENSMUST00000028897; ENSMUSP00000028897; ENSMUSG00000027408.
GeneIDi56264.
KEGGimmu:56264.
UCSCiuc008mir.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077738 mRNA. Translation: AAD15985.1.
BX890605 Genomic DNA. Translation: CAM20117.1.
CH466519 Genomic DNA. Translation: EDL28262.1.
BC003713 mRNA. Translation: AAH03713.1.
CCDSiCCDS16740.1.
RefSeqiNP_062670.2. NM_019696.2.
UniGeneiMm.112701.

3D structure databases

ProteinModelPortaliQ9Z100.
SMRiQ9Z100. Positions 107-263, 284-667.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028897.

Protein family/group databases

MEROPSiM14.952.

PTM databases

PhosphoSiteiQ9Z100.

Proteomic databases

PRIDEiQ9Z100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028897; ENSMUSP00000028897; ENSMUSG00000027408.
GeneIDi56264.
KEGGimmu:56264.
UCSCiuc008mir.2. mouse.

Organism-specific databases

CTDi56265.
MGIiMGI:1934569. Cpxm1.

Phylogenomic databases

eggNOGiNOG322453.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiQ9Z100.
KOiK08638.
OMAiRYDWVTS.
OrthoDBiEOG7B8S32.
TreeFamiTF315592.

Miscellaneous databases

NextBioi312152.
PROiQ9Z100.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z100.
ExpressionAtlasiQ9Z100. baseline and differential.
GenevestigatoriQ9Z100.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR008979. Galactose-bd-like.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00231. FA58C. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
SSF49785. SSF49785. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
PS01285. FA58C_1. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of mouse CPX-1, a novel member of the metallocarboxypeptidase gene family with highest similarity to CPX-2."
    Lei Y., Xin X., Morgan D., Pintar J.E., Fricker L.D.
    DNA Cell Biol. 18:175-185(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Heart.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.

Entry informationi

Entry nameiCPXM1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z100
Secondary accession number(s): A2BI86, Q99LA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: July 27, 2011
Last modified: January 7, 2015
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.