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Q9Z100 (CPXM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable carboxypeptidase X1

EC=3.4.17.-
Alternative name(s):
Metallocarboxypeptidase CPX-1
Gene names
Name:Cpxm1
Synonyms:Cpx1, Cpxm
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length722 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May be involved in cell-cell interactions. No carboxypeptidase activity was found yet.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted Probable.

Tissue specificity

Strongly expressed in testis and spleen. Moderatly expressed in salivary gland, brain, heart, lung, and kidney. Extremely low expression in liver and muscle. No expression in eye, adrenal, and white adipose tissues. Ref.1

Developmental stage

First expressed at 13.5 dpc, in the meninges, nasal mesenchyme, primordial cartilage and skeletal structures. Ref.1

Sequence similarities

Belongs to the peptidase M14 family.

Contains 1 F5/8 type C domain.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionCarboxypeptidase
Hydrolase
Metalloprotease
Protease
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell adhesion

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular space

Inferred from direct assay Ref.1. Source: MGI

   Molecular_functionhydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 722702Probable carboxypeptidase X1
PRO_0000004408

Regions

Domain103 – 263161F5/8 type C
Compositional bias62 – 654Poly-Lys
Compositional bias358 – 3625Poly-Leu

Sites

Active site5801Nucleophile By similarity
Metal binding3491Zinc By similarity
Metal binding3521Zinc By similarity
Metal binding4871Zinc By similarity

Amino acid modifications

Glycosylation491N-linked (GlcNAc...) Potential
Glycosylation2001N-linked (GlcNAc...) Potential
Glycosylation2101N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation4611N-linked (GlcNAc...) Potential
Disulfide bond105 ↔ 263 By similarity

Experimental info

Sequence conflict2531A → V in AAD15985. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9Z100 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: CC365C119420A9D4

FASTA72280,907
        10         20         30         40         50         60 
MWGLLLAVTA FAPSVGLGLG APSASVPGLA PGSTLAPHSS VAQPSTKANE TSERHVRLRV 

        70         80         90        100        110        120 
IKKKKIVVKK RKKLRHPGPL GTARPVVPTH PAKTLTLPEK QEPGCPPLGL ESLRVSDSQL 

       130        140        150        160        170        180 
EASSSQSFGL GAHRGRLNIQ SGLEDGDLYD GAWCAEQQDT EPWLQVDAKN PVRFAGIVTQ 

       190        200        210        220        230        240 
GRNSVWRYDW VTSFKVQFSN DSQTWWKSRN STGMDIVFPA NSDAETPVLN LLPEPQVARF 

       250        260        270        280        290        300 
IRLLPQTWFQ GGAPCLRAEI LACPVSDPND LFPEAHTLGS SNSLDFRHHN YKAMRKLMKQ 

       310        320        330        340        350        360 
VNEQCPNITR IYSIGKSHQG LKLYVMEMSD HPGEHELGEP EVRYVAGMHG NEALGRELLL 

       370        380        390        400        410        420 
LLMQFLCHEF LRGDPRVTRL LTETRIHLLP SMNPDGYETA YHRGSELVGW AEGRWTHQGI 

       430        440        450        460        470        480 
DLNHNFADLN TQLWYAEDDG LVPDTVPNHH LPLPTYYTLP NATVAPETWA VIKWMKRIPF 

       490        500        510        520        530        540 
VLSANLHGGE LVVSYPFDMT RTPWAARELT PTPDDAVFRW LSTVYAGTNR AMQDTDRRPC 

       550        560        570        580        590        600 
HSQDFSLHGN VINGADWHTV PGSMNDFSYL HTNCFEVTVE LSCDKFPHEK ELPQEWENNK 

       610        620        630        640        650        660 
DALLTYLEQV RMGITGVVRD KDTELGIADA VIAVEGINHD VTTAWGGDYW RLLTPGDYVV 

       670        680        690        700        710        720 
TASAEGYHTV RQHCQVTFEE GPVPCNFLLT KTPKERLREL LATRGKLPPD LRRKLERLRG 


QK 

« Hide

References

« Hide 'large scale' references
[1]"Identification of mouse CPX-1, a novel member of the metallocarboxypeptidase gene family with highest similarity to CPX-2."
Lei Y., Xin X., Morgan D., Pintar J.E., Fricker L.D.
DNA Cell Biol. 18:175-185(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Heart.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF077738 mRNA. Translation: AAD15985.1.
BX890605 Genomic DNA. Translation: CAM20117.1.
CH466519 Genomic DNA. Translation: EDL28262.1.
BC003713 mRNA. Translation: AAH03713.1.
CCDSCCDS16740.1.
RefSeqNP_062670.2. NM_019696.2.
UniGeneMm.112701.

3D structure databases

ProteinModelPortalQ9Z100.
SMRQ9Z100. Positions 107-263, 284-667.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000028897.

Protein family/group databases

MEROPSM14.952.

PTM databases

PhosphoSiteQ9Z100.

Proteomic databases

PRIDEQ9Z100.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028897; ENSMUSP00000028897; ENSMUSG00000027408.
GeneID56264.
KEGGmmu:56264.
UCSCuc008mir.2. mouse.

Organism-specific databases

CTD56265.
MGIMGI:1934569. Cpxm1.

Phylogenomic databases

eggNOGNOG322453.
GeneTreeENSGT00730000110373.
HOGENOMHOG000232185.
HOVERGENHBG003410.
InParanoidA2BI86.
KOK08638.
OMARYDWVTS.
OrthoDBEOG7B8S32.
TreeFamTF315592.

Gene expression databases

ArrayExpressQ9Z100.
BgeeQ9Z100.
GenevestigatorQ9Z100.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
InterProIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR008979. Galactose-bd-like.
IPR000834. Peptidase_M14.
[Graphical view]
PfamPF00754. F5_F8_type_C. 1 hit.
PF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSPR00765. CRBOXYPTASEA.
SMARTSM00231. FA58C. 1 hit.
SM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMSSF49464. SSF49464. 1 hit.
SSF49785. SSF49785. 1 hit.
PROSITEPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
PS01285. FA58C_1. 1 hit.
PS50022. FA58C_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio312152.
PROQ9Z100.
SOURCESearch...

Entry information

Entry nameCPXM1_MOUSE
AccessionPrimary (citable) accession number: Q9Z100
Secondary accession number(s): A2BI86, Q99LA3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot