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Protein

Oxysterols receptor LXR-alpha

Gene

Nr1h3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nuclear receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Exhibits a ligand-dependent transcriptional activation activity (By similarity).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi93 – 168Nuclear receptorPROSITE-ProRule annotationAdd BLAST76
Zinc fingeri96 – 116NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri132 – 156NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

  • apoptotic cell clearance Source: MGI
  • cellular lipid metabolic process Source: MGI
  • cellular response to lipopolysaccharide Source: BHF-UCL
  • cholesterol homeostasis Source: BHF-UCL
  • fatty acid biosynthetic process Source: BHF-UCL
  • lipid homeostasis Source: BHF-UCL
  • negative regulation of cholesterol storage Source: BHF-UCL
  • negative regulation of inflammatory response Source: BHF-UCL
  • negative regulation of lipid transport Source: MGI
  • negative regulation of macrophage activation Source: BHF-UCL
  • negative regulation of pancreatic juice secretion Source: BHF-UCL
  • negative regulation of pinocytosis Source: MGI
  • negative regulation of proteolysis Source: BHF-UCL
  • negative regulation of secretion of lysosomal enzymes Source: BHF-UCL
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of cellular protein metabolic process Source: MGI
  • positive regulation of cholesterol efflux Source: BHF-UCL
  • positive regulation of cholesterol homeostasis Source: MGI
  • positive regulation of cholesterol transport Source: MGI
  • positive regulation of fatty acid biosynthetic process Source: MGI
  • positive regulation of lipoprotein lipase activity Source: MGI
  • positive regulation of receptor biosynthetic process Source: MGI
  • positive regulation of toll-like receptor 4 signaling pathway Source: MGI
  • positive regulation of transcription, DNA-templated Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • positive regulation of triglyceride biosynthetic process Source: MGI
  • regulation of cholesterol homeostasis Source: UniProtKB
  • regulation of transcription, DNA-templated Source: MGI
  • response to progesterone Source: MGI
  • sterol homeostasis Source: BHF-UCL
  • triglyceride homeostasis Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Activator, Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.
R-MMU-8866427. VLDLR internalisation and degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxysterols receptor LXR-alpha
Alternative name(s):
Liver X receptor alpha
Nuclear receptor subfamily 1 group H member 3
Gene namesi
Name:Nr1h3
Synonyms:Lxra
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1352462. Nr1h3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • nuclear chromatin Source: MGI
  • nucleus Source: MGI
  • receptor complex Source: MGI
  • RNA polymerase II transcription factor complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2189152.
GuidetoPHARMACOLOGYi602.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000535361 – 445Oxysterols receptor LXR-alphaAdd BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z0Y9.
PaxDbiQ9Z0Y9.
PRIDEiQ9Z0Y9.

PTM databases

iPTMnetiQ9Z0Y9.
PhosphoSitePlusiQ9Z0Y9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000002108.
CleanExiMM_NR1H3.
ExpressionAtlasiQ9Z0Y9. baseline and differential.
GenevisibleiQ9Z0Y9. MM.

Interactioni

Subunit structurei

Heterodimer of LXRA and RXR. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with SIRT1 and this interaction is inhibited by CCAR2 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Men1O885593EBI-5276764,EBI-3990176
SIRT1Q96EB62EBI-5276764,EBI-1802965From a different organism.

Protein-protein interaction databases

BioGridi204449. 6 interactors.
IntActiQ9Z0Y9. 5 interactors.
STRINGi10090.ENSMUSP00000002177.

Structurei

Secondary structure

1445
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi206 – 227Combined sources22
Helixi228 – 232Combined sources5
Helixi245 – 272Combined sources28
Turni274 – 278Combined sources5
Helixi281 – 301Combined sources21
Turni306 – 309Combined sources4
Beta strandi310 – 313Combined sources4
Turni314 – 316Combined sources3
Beta strandi317 – 319Combined sources3
Helixi321 – 325Combined sources5
Turni326 – 328Combined sources3
Helixi331 – 347Combined sources17
Helixi351 – 362Combined sources12
Helixi373 – 394Combined sources22
Helixi401 – 428Combined sources28
Helixi435 – 441Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ACLX-ray2.80B/D/F/H203-445[»]
3FALX-ray2.36B/D200-445[»]
3FC6X-ray2.06B/D200-445[»]
ProteinModelPortaliQ9Z0Y9.
SMRiQ9Z0Y9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Z0Y9.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 94Transactivation AF-1; required for ligand-independent transactivation functionBy similarityAdd BLAST94
Regioni203 – 445Transactivation AF-2; required for ligand-dependent transactivation function; mediates interaction with CCAR2By similarityAdd BLAST243
Regioni213 – 432Ligand-bindingSequence analysisAdd BLAST220

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri96 – 116NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri132 – 156NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00850000132242.
HOGENOMiHOG000220845.
HOVERGENiHBG108655.
InParanoidiQ9Z0Y9.
KOiK08536.
OMAiCILREEA.
OrthoDBiEOG091G0I4P.
TreeFamiTF352167.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR023257. Liver_X_rcpt.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR02034. LIVERXRECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z0Y9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLWLEASMP DVSPDSATEL WKTEPQDAGD QGGNTCILRE EARMPQSTGV
60 70 80 90 100
ALGIGLESAE PTALLPRAET LPEPTELRPQ KRKKGPAPKM LGNELCSVCG
110 120 130 140 150
DKASGFHYNV LSCEGCKGFF RRSVIKGARY VCHSGGHCPM DTYMRRKCQE
160 170 180 190 200
CRLRKCRQAG MREECVLSEE QIRLKKLKRQ EEEQAQATSV SPRVSSPPQV
210 220 230 240 250
LPQLSPEQLG MIEKLVAAQQ QCNRRSFSDR LRVTPWPIAP DPQSREARQQ
260 270 280 290 300
RFAHFTELAI VSVQEIVDFA KQLPGFLQLS REDQIALLKT SAIEVMLLET
310 320 330 340 350
SRRYNPGSES ITFLKDFSYN REDFAKAGLQ VEFINPIFEF SRAMNELQLN
360 370 380 390 400
DAEFALLIAI SIFSADRPNV QDQLQVERLQ HTYVEALHAY VSINHPHDPL
410 420 430 440
MFPRMLMKLV SLRTLSSVHS EQVFALRLQD KKLPPLLSEI WDVHE
Length:445
Mass (Da):50,418
Last modified:July 27, 2011 - v3
Checksum:i1A426DF38D935731
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti399P → R in CAB51952 (PubMed:10675617).Curated1
Sequence conflicti399P → R in CAB51923 (PubMed:10675617).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132599, AJ132600 Genomic DNA. Translation: CAB51952.1.
AJ132601 mRNA. Translation: CAB51923.1.
AF085745 mRNA. Translation: AAD16050.1.
AL691450 Genomic DNA. No translation available.
CCDSiCCDS16426.1.
RefSeqiNP_001171201.1. NM_001177730.1.
NP_038867.2. NM_013839.4.
XP_006499229.1. XM_006499166.2.
XP_006499230.1. XM_006499167.2.
XP_006499231.1. XM_006499168.3.
UniGeneiMm.22690.

Genome annotation databases

EnsembliENSMUST00000002177; ENSMUSP00000002177; ENSMUSG00000002108.
ENSMUST00000111354; ENSMUSP00000106986; ENSMUSG00000002108.
ENSMUST00000111356; ENSMUSP00000106988; ENSMUSG00000002108.
GeneIDi22259.
KEGGimmu:22259.
UCSCiuc008kvc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132599, AJ132600 Genomic DNA. Translation: CAB51952.1.
AJ132601 mRNA. Translation: CAB51923.1.
AF085745 mRNA. Translation: AAD16050.1.
AL691450 Genomic DNA. No translation available.
CCDSiCCDS16426.1.
RefSeqiNP_001171201.1. NM_001177730.1.
NP_038867.2. NM_013839.4.
XP_006499229.1. XM_006499166.2.
XP_006499230.1. XM_006499167.2.
XP_006499231.1. XM_006499168.3.
UniGeneiMm.22690.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ACLX-ray2.80B/D/F/H203-445[»]
3FALX-ray2.36B/D200-445[»]
3FC6X-ray2.06B/D200-445[»]
ProteinModelPortaliQ9Z0Y9.
SMRiQ9Z0Y9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204449. 6 interactors.
IntActiQ9Z0Y9. 5 interactors.
STRINGi10090.ENSMUSP00000002177.

Chemistry databases

ChEMBLiCHEMBL2189152.
GuidetoPHARMACOLOGYi602.

PTM databases

iPTMnetiQ9Z0Y9.
PhosphoSitePlusiQ9Z0Y9.

Proteomic databases

MaxQBiQ9Z0Y9.
PaxDbiQ9Z0Y9.
PRIDEiQ9Z0Y9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002177; ENSMUSP00000002177; ENSMUSG00000002108.
ENSMUST00000111354; ENSMUSP00000106986; ENSMUSG00000002108.
ENSMUST00000111356; ENSMUSP00000106988; ENSMUSG00000002108.
GeneIDi22259.
KEGGimmu:22259.
UCSCiuc008kvc.2. mouse.

Organism-specific databases

CTDi10062.
MGIiMGI:1352462. Nr1h3.

Phylogenomic databases

eggNOGiKOG3575. Eukaryota.
ENOG410XRZC. LUCA.
GeneTreeiENSGT00850000132242.
HOGENOMiHOG000220845.
HOVERGENiHBG108655.
InParanoidiQ9Z0Y9.
KOiK08536.
OMAiCILREEA.
OrthoDBiEOG091G0I4P.
TreeFamiTF352167.

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.
R-MMU-8866427. VLDLR internalisation and degradation.

Miscellaneous databases

EvolutionaryTraceiQ9Z0Y9.
PROiQ9Z0Y9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002108.
CleanExiMM_NR1H3.
ExpressionAtlasiQ9Z0Y9. baseline and differential.
GenevisibleiQ9Z0Y9. MM.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR023257. Liver_X_rcpt.
IPR000536. Nucl_hrmn_rcpt_lig-bd.
IPR001723. Nuclear_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR02034. LIVERXRECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNR1H3_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0Y9
Secondary accession number(s): Q9QUH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.