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Q9Z0Y9

- NR1H3_MOUSE

UniProt

Q9Z0Y9 - NR1H3_MOUSE

Protein

Oxysterols receptor LXR-alpha

Gene

Nr1h3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Nuclear receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi93 – 16876Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri96 – 11621NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri132 – 15625NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: MGI
    3. protein binding Source: IntAct
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    6. sequence-specific DNA binding transcription factor activity Source: MGI
    7. steroid hormone receptor activity Source: InterPro
    8. sterol response element binding Source: Ensembl
    9. zinc ion binding Source: InterPro

    GO - Biological processi

    1. apoptotic cell clearance Source: Ensembl
    2. cellular lipid metabolic process Source: MGI
    3. cellular response to lipopolysaccharide Source: BHF-UCL
    4. cholesterol homeostasis Source: BHF-UCL
    5. fatty acid biosynthetic process Source: BHF-UCL
    6. intracellular receptor signaling pathway Source: GOC
    7. lipid homeostasis Source: BHF-UCL
    8. negative regulation of cholesterol storage Source: BHF-UCL
    9. negative regulation of inflammatory response Source: BHF-UCL
    10. negative regulation of lipid transport Source: Ensembl
    11. negative regulation of macrophage activation Source: BHF-UCL
    12. negative regulation of pancreatic juice secretion Source: BHF-UCL
    13. negative regulation of pinocytosis Source: Ensembl
    14. negative regulation of proteolysis Source: BHF-UCL
    15. negative regulation of secretion of lysosomal enzymes Source: BHF-UCL
    16. negative regulation of transcription, DNA-templated Source: MGI
    17. negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    18. positive regulation of cellular protein metabolic process Source: Ensembl
    19. positive regulation of cholesterol efflux Source: BHF-UCL
    20. positive regulation of cholesterol homeostasis Source: Ensembl
    21. positive regulation of fatty acid biosynthetic process Source: Ensembl
    22. positive regulation of lipoprotein lipase activity Source: Ensembl
    23. positive regulation of receptor biosynthetic process Source: Ensembl
    24. positive regulation of toll-like receptor 4 signaling pathway Source: Ensembl
    25. positive regulation of transcription, DNA-templated Source: BHF-UCL
    26. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    27. positive regulation of triglyceride biosynthetic process Source: Ensembl
    28. regulation of cholesterol homeostasis Source: UniProtKB
    29. regulation of transcription, DNA-templated Source: MGI
    30. response to progesterone Source: Ensembl
    31. sterol homeostasis Source: BHF-UCL
    32. triglyceride homeostasis Source: BHF-UCL

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oxysterols receptor LXR-alpha
    Alternative name(s):
    Liver X receptor alpha
    Nuclear receptor subfamily 1 group H member 3
    Gene namesi
    Name:Nr1h3
    Synonyms:Lxra
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1352462. Nr1h3.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. nuclear chromatin Source: Ensembl
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 445445Oxysterols receptor LXR-alphaPRO_0000053536Add
    BLAST

    Proteomic databases

    PRIDEiQ9Z0Y9.

    PTM databases

    PhosphoSiteiQ9Z0Y9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Z0Y9.
    BgeeiQ9Z0Y9.
    CleanExiMM_NR1H3.
    GenevestigatoriQ9Z0Y9.

    Interactioni

    Subunit structurei

    Heterodimer of LXRA and RXR.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SIRT1Q96EB62EBI-5276764,EBI-1802965From a different organism.

    Protein-protein interaction databases

    BioGridi204449. 7 interactions.
    IntActiQ9Z0Y9. 2 interactions.

    Structurei

    Secondary structure

    1
    445
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi206 – 22722
    Helixi228 – 2325
    Helixi245 – 27228
    Turni274 – 2785
    Helixi281 – 30121
    Turni306 – 3094
    Beta strandi310 – 3134
    Turni314 – 3163
    Beta strandi317 – 3193
    Helixi321 – 3255
    Turni326 – 3283
    Helixi331 – 34717
    Helixi351 – 36212
    Helixi373 – 39422
    Helixi401 – 42828
    Helixi435 – 4417

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ACLX-ray2.80B/D/F/H203-445[»]
    3FALX-ray2.36B/D200-445[»]
    3FC6X-ray2.06B/D200-445[»]
    ProteinModelPortaliQ9Z0Y9.
    SMRiQ9Z0Y9. Positions 83-443.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9Z0Y9.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni213 – 432220Ligand-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri96 – 11621NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri132 – 15625NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG285805.
    GeneTreeiENSGT00720000108423.
    HOGENOMiHOG000220845.
    HOVERGENiHBG108655.
    InParanoidiQ9Z0Y9.
    KOiK08536.
    OMAiCILREEA.
    OrthoDBiEOG7DC25S.
    TreeFamiTF352167.

    Family and domain databases

    Gene3Di1.10.565.10. 2 hits.
    3.30.50.10. 1 hit.
    InterProiIPR023257. Liver_X_rcpt.
    IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR02034. LIVERXRECPTR.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z0Y9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLWLEASMP DVSPDSATEL WKTEPQDAGD QGGNTCILRE EARMPQSTGV    50
    ALGIGLESAE PTALLPRAET LPEPTELRPQ KRKKGPAPKM LGNELCSVCG 100
    DKASGFHYNV LSCEGCKGFF RRSVIKGARY VCHSGGHCPM DTYMRRKCQE 150
    CRLRKCRQAG MREECVLSEE QIRLKKLKRQ EEEQAQATSV SPRVSSPPQV 200
    LPQLSPEQLG MIEKLVAAQQ QCNRRSFSDR LRVTPWPIAP DPQSREARQQ 250
    RFAHFTELAI VSVQEIVDFA KQLPGFLQLS REDQIALLKT SAIEVMLLET 300
    SRRYNPGSES ITFLKDFSYN REDFAKAGLQ VEFINPIFEF SRAMNELQLN 350
    DAEFALLIAI SIFSADRPNV QDQLQVERLQ HTYVEALHAY VSINHPHDPL 400
    MFPRMLMKLV SLRTLSSVHS EQVFALRLQD KKLPPLLSEI WDVHE 445
    Length:445
    Mass (Da):50,418
    Last modified:July 27, 2011 - v3
    Checksum:i1A426DF38D935731
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti399 – 3991P → R in CAB51952. (PubMed:10675617)Curated
    Sequence conflicti399 – 3991P → R in CAB51923. (PubMed:10675617)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ132599, AJ132600 Genomic DNA. Translation: CAB51952.1.
    AJ132601 mRNA. Translation: CAB51923.1.
    AF085745 mRNA. Translation: AAD16050.1.
    AL691450 Genomic DNA. No translation available.
    CCDSiCCDS16426.1.
    RefSeqiNP_001171201.1. NM_001177730.1.
    NP_038867.2. NM_013839.4.
    XP_006499229.1. XM_006499166.1.
    XP_006499230.1. XM_006499167.1.
    XP_006499231.1. XM_006499168.1.
    UniGeneiMm.22690.

    Genome annotation databases

    EnsembliENSMUST00000002177; ENSMUSP00000002177; ENSMUSG00000002108.
    ENSMUST00000111354; ENSMUSP00000106986; ENSMUSG00000002108.
    ENSMUST00000111356; ENSMUSP00000106988; ENSMUSG00000002108.
    GeneIDi22259.
    KEGGimmu:22259.
    UCSCiuc008kvc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ132599 , AJ132600 Genomic DNA. Translation: CAB51952.1 .
    AJ132601 mRNA. Translation: CAB51923.1 .
    AF085745 mRNA. Translation: AAD16050.1 .
    AL691450 Genomic DNA. No translation available.
    CCDSi CCDS16426.1.
    RefSeqi NP_001171201.1. NM_001177730.1.
    NP_038867.2. NM_013839.4.
    XP_006499229.1. XM_006499166.1.
    XP_006499230.1. XM_006499167.1.
    XP_006499231.1. XM_006499168.1.
    UniGenei Mm.22690.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ACL X-ray 2.80 B/D/F/H 203-445 [» ]
    3FAL X-ray 2.36 B/D 200-445 [» ]
    3FC6 X-ray 2.06 B/D 200-445 [» ]
    ProteinModelPortali Q9Z0Y9.
    SMRi Q9Z0Y9. Positions 83-443.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204449. 7 interactions.
    IntActi Q9Z0Y9. 2 interactions.

    Chemistry

    ChEMBLi CHEMBL2189152.

    PTM databases

    PhosphoSitei Q9Z0Y9.

    Proteomic databases

    PRIDEi Q9Z0Y9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002177 ; ENSMUSP00000002177 ; ENSMUSG00000002108 .
    ENSMUST00000111354 ; ENSMUSP00000106986 ; ENSMUSG00000002108 .
    ENSMUST00000111356 ; ENSMUSP00000106988 ; ENSMUSG00000002108 .
    GeneIDi 22259.
    KEGGi mmu:22259.
    UCSCi uc008kvc.2. mouse.

    Organism-specific databases

    CTDi 10062.
    MGIi MGI:1352462. Nr1h3.

    Phylogenomic databases

    eggNOGi NOG285805.
    GeneTreei ENSGT00720000108423.
    HOGENOMi HOG000220845.
    HOVERGENi HBG108655.
    InParanoidi Q9Z0Y9.
    KOi K08536.
    OMAi CILREEA.
    OrthoDBi EOG7DC25S.
    TreeFami TF352167.

    Miscellaneous databases

    EvolutionaryTracei Q9Z0Y9.
    NextBioi 302349.
    PROi Q9Z0Y9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z0Y9.
    Bgeei Q9Z0Y9.
    CleanExi MM_NR1H3.
    Genevestigatori Q9Z0Y9.

    Family and domain databases

    Gene3Di 1.10.565.10. 2 hits.
    3.30.50.10. 1 hit.
    InterProi IPR023257. Liver_X_rcpt.
    IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR02034. LIVERXRECPTR.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural characterisation of the mouse nuclear oxysterol receptor genes LXRalpha and LXRbeta."
      Alberti S., Steffensen K.R., Gustafsson J.-A.
      Gene 243:93-103(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/SvJ.
      Tissue: Liver.
    2. "LXRalpha functions as a cAMP-responsive transcriptional regulator of gene expression."
      Tamura K., Chen Y.E., Horiuchi M., Chen Q., Daviet L., Yang Z., Lopez-Ilasaca M., Mu H., Pratt R.E., Dzau V.J.
      Proc. Natl. Acad. Sci. U.S.A. 97:8513-8518(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "Identification of oxysterol 7alpha-hydroxylase (Cyp7b1) as a novel retinoid-related orphan receptor alpha (RORalpha) (NR1F1) target gene and a functional cross-talk between RORalpha and liver X receptor (NR1H3)."
      Wada T., Kang H.S., Angers M., Gong H., Bhatia S., Khadem S., Ren S., Ellis E., Strom S.C., Jetten A.M., Xie W.
      Mol. Pharmacol. 73:891-899(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN METABOLISM REGULATION.
    5. "LXR regulates cholesterol uptake through Idol-dependent ubiquitination of the LDL receptor."
      Zelcer N., Hong C., Boyadjian R., Tontonoz P.
      Science 325:100-104(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The E3 ubiquitin ligase IDOL induces the degradation of the low density lipoprotein receptor family members VLDLR and ApoER2."
      Hong C., Duit S., Jalonen P., Out R., Scheer L., Sorrentino V., Boyadjian R., Rodenburg K.W., Foley E., Korhonen L., Lindholm D., Nimpf J., van Berkel T.J., Tontonoz P., Zelcer N.
      J. Biol. Chem. 285:19720-19726(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiNR1H3_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z0Y9
    Secondary accession number(s): Q9QUH7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3