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Protein

Voltage-dependent T-type calcium channel subunit alpha-1I

Gene

Cacna1i

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This channel gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. Gates in voltage ranges similar to, but higher than alpha 1G or alpha 1H.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei355Calcium ion selectivity and permeabilityBy similarity1
Sitei779Calcium ion selectivity and permeabilityBy similarity1
Sitei1339Calcium ion selectivity and permeabilityBy similarity1
Sitei1637Calcium ion selectivity and permeabilityBy similarity1

GO - Molecular functioni

  • low voltage-gated calcium channel activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent T-type calcium channel subunit alpha-1I
Alternative name(s):
CaVT.3
Voltage-gated calcium channel subunit alpha Cav3.3
Gene namesi
Name:Cacna1i
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68944. Cacna1i.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 76CytoplasmicSequence analysisAdd BLAST76
Transmembranei77 – 97Helical; Name=S1 of repeat ISequence analysisAdd BLAST21
Topological domaini98 – 115ExtracellularSequence analysisAdd BLAST18
Transmembranei116 – 137Helical; Name=S2 of repeat ISequence analysisAdd BLAST22
Topological domaini138 – 146CytoplasmicSequence analysis9
Transmembranei147 – 166Helical; Name=S3 of repeat ISequence analysisAdd BLAST20
Topological domaini167 – 171ExtracellularSequence analysis5
Transmembranei172 – 189Helical; Name=S4 of repeat ISequence analysisAdd BLAST18
Topological domaini190 – 209CytoplasmicSequence analysisAdd BLAST20
Transmembranei210 – 230Helical; Name=S5 of repeat ISequence analysisAdd BLAST21
Topological domaini231 – 371ExtracellularSequence analysisAdd BLAST141
Transmembranei372 – 396Helical; Name=S6 of repeat ISequence analysisAdd BLAST25
Topological domaini397 – 598CytoplasmicSequence analysisAdd BLAST202
Transmembranei599 – 619Helical; Name=S1 of repeat IISequence analysisAdd BLAST21
Topological domaini620 – 632ExtracellularSequence analysisAdd BLAST13
Transmembranei633 – 654Helical; Name=S2 of repeat IISequence analysisAdd BLAST22
Topological domaini655 – 660CytoplasmicSequence analysis6
Transmembranei661 – 679Helical; Name=S3 of repeat IISequence analysisAdd BLAST19
Topological domaini680 – 687ExtracellularSequence analysis8
Transmembranei688 – 711Helical; Name=S4 of repeat IISequence analysisAdd BLAST24
Topological domaini712 – 722CytoplasmicSequence analysisAdd BLAST11
Transmembranei723 – 743Helical; Name=S5 of repeat IISequence analysisAdd BLAST21
Topological domaini744 – 795ExtracellularSequence analysisAdd BLAST52
Transmembranei796 – 820Helical; Name=S6 of repeat IISequence analysisAdd BLAST25
Topological domaini821 – 1125CytoplasmicSequence analysisAdd BLAST305
Transmembranei1126 – 1148Helical; Name=S1 of repeat IIISequence analysisAdd BLAST23
Topological domaini1149 – 1166ExtracellularSequence analysisAdd BLAST18
Transmembranei1167 – 1187Helical; Name=S2 of repeat IIISequence analysisAdd BLAST21
Topological domaini1188 – 1197CytoplasmicSequence analysis10
Transmembranei1198 – 1217Helical; Name=S3 of repeat IIISequence analysisAdd BLAST20
Topological domaini1218 – 1231ExtracellularSequence analysisAdd BLAST14
Transmembranei1232 – 1253Helical; Name=S4 of repeat IIISequence analysisAdd BLAST22
Topological domaini1254 – 1263CytoplasmicSequence analysis10
Transmembranei1264 – 1287Helical; Name=S5 of repeat IIISequence analysisAdd BLAST24
Topological domaini1288 – 1364ExtracellularSequence analysisAdd BLAST77
Transmembranei1365 – 1390Helical; Name=S6 of repeat IIISequence analysisAdd BLAST26
Topological domaini1391 – 1445CytoplasmicSequence analysisAdd BLAST55
Transmembranei1446 – 1466Helical; Name=S1 of repeat IVSequence analysisAdd BLAST21
Topological domaini1467 – 1480ExtracellularSequence analysisAdd BLAST14
Transmembranei1481 – 1502Helical; Name=S2 of repeat IVSequence analysisAdd BLAST22
Topological domaini1503 – 1509CytoplasmicSequence analysis7
Transmembranei1510 – 1528Helical; Name=S3 of repeat IVSequence analysisAdd BLAST19
Topological domaini1529 – 1542ExtracellularSequence analysisAdd BLAST14
Transmembranei1543 – 1566Helical; Name=S4 of repeat IVSequence analysisAdd BLAST24
Topological domaini1567 – 1580CytoplasmicSequence analysisAdd BLAST14
Transmembranei1581 – 1601Helical; Name=S5 of repeat IVSequence analysisAdd BLAST21
Topological domaini1602 – 1664ExtracellularSequence analysisAdd BLAST63
Transmembranei1665 – 1692Helical; Name=S6 of repeat IVSequence analysisAdd BLAST28
Topological domaini1693 – 1835CytoplasmicSequence analysisAdd BLAST143

GO - Cellular componenti

  • plasma membrane Source: GO_Central
  • voltage-gated calcium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi537.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000539581 – 2201Voltage-dependent T-type calcium channel subunit alpha-1IAdd BLAST2201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi171N-linked (GlcNAc...)Sequence analysis1
Glycosylationi242N-linked (GlcNAc...)Sequence analysis1
Glycosylationi309N-linked (GlcNAc...)Sequence analysis1
Modified residuei1017PhosphoserineCombined sources1
Glycosylationi1301N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1304N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

In response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9Z0Y8.
PRIDEiQ9Z0Y8.

PTM databases

iPTMnetiQ9Z0Y8.
PhosphoSitePlusiQ9Z0Y8.

Expressioni

Tissue specificityi

Brain.

Interactioni

Subunit structurei

Interacts with CATSPER1 and CATSPER2, leading to suppress T-type calcium channel activity.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000046091.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0Y8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati64 – 399IAdd BLAST336
Repeati584 – 823IIAdd BLAST240
Repeati1116 – 1393IIIAdd BLAST278
Repeati1431 – 1692IVAdd BLAST262

Domaini

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2302. Eukaryota.
ENOG410XNP6. LUCA.
HOVERGENiHBG050764.
InParanoidiQ9Z0Y8.
KOiK04856.
PhylomeDBiQ9Z0Y8.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR030162. CACNA1I.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR005445. VDCC_T_a1.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF209. PTHR10037:SF209. 3 hits.
PfamiPF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01629. TVDCCALPHA1.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z0Y8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADSNLPPSS AAAPAPEPGI TEQPGPRSPP PSPPGLEEPL EGTNPDVPHP
60 70 80 90 100
DLAPVAFFCL RQTTSPRNWC IKMVCNPWFE CVSMLVILLN CVTLGMYQPC
110 120 130 140 150
DDMECLSDRC KILQVFDDFI FIFFAMEMVL KMVALGIFGK KCYLGDTWNR
160 170 180 190 200
LDFFIVMAGM VEYSLDLQNI NLSAIRTVRV LRPLKAINRV PSMRILVNLL
210 220 230 240 250
LDTLPMLGNV LLLCFFVFFI FGIIGVQLWA GLLRNRCFLE ENFTIQGDVA
260 270 280 290 300
LPPYYQPEED DEMPFICSLT GDNGIMGCHE IPPLKEQGRE CCLSKDDVYD
310 320 330 340 350
FGAGRQDLNA SGLCVNWNRY YNVCRTGNAN PHKGAINFDN IGYAWIVIFQ
360 370 380 390 400
VITLEGWVEI MYYVMDAHSF YNFIYFILLI IVGSFFMINL CLVVIATQFS
410 420 430 440 450
ETKQREHRLM LEQRQRYLSS STVASYAEPG DCYEEIFQYV CHILRKAKRR
460 470 480 490 500
ALGLYQALQN RRQAMGPGTP APAKPGPHAK EPSHCKLCPR HSPLDPTPHT
510 520 530 540 550
LVQPISAILA SDPSSCPHCQ HEAGRRPSGL GSTDSGQEGS GSGGSAEAEA
560 570 580 590 600
NGDGPQSSED GVSSDLGKEE EQEDGAARLC GDVWRETREK LRGIVDSKYF
610 620 630 640 650
NRGIMMAILV NTVSMGIEHH EQPEELTNIL EICNVVFTSM FALEMILKLA
660 670 680 690 700
AFGLFDYLRN PYNIFDSIIV IISIWEIVGQ ADGGLSVLRT FRLLRVLKLV
710 720 730 740 750
RFMPALRRQL VVLMKTMDNV ATFCMLLMLF IFIFSILGMH IFGCKFSLRT
760 770 780 790 800
DTGDTVPDRK NFDSLLWAIV TVFQILTQED WNVVLYNGMA STTPWASLYF
810 820 830 840 850
VALMTFGNYV LFNLLVAILV EGFQAEGDAN RSYSDEDQSS SNLEEFDKLP
860 870 880 890 900
EGLDNSRDLK LCPIPMTPNG HLDPSLPLGA HLGPAGTMGT APRLSLQPDP
910 920 930 940 950
VLVALDSRKS SVMSLGRMSY DQRSLSSSRS SYYGPWGRSG TWASRRSSWN
960 970 980 990 1000
SLKHKPPSAE HESLLSGEGG GSCVRACEGA REEAPTRTAP LHAPHAHHAH
1010 1020 1030 1040 1050
HGPHLAHRHR HHRRTLSLDT RDSVDLGELV PVVGAHSRAA WRGAGQAPGH
1060 1070 1080 1090 1100
EDCNGRMPNI AKDVFTKMDD CRDRGEDEEE IDYTLCFRVR KMIDVYKPDW
1110 1120 1130 1140 1150
CEVREDWSVY LFSPENKFRI LCQTIIAHKL FDYVVLAFIF LNCITIALER
1160 1170 1180 1190 1200
PQIEAGSTER IFLTVSNYIF TAIFVGEMTL KVVSLGLYFG EQAYLRSSWN
1210 1220 1230 1240 1250
VLDGFLVFVS IIDIVVSVAS AGGAKILGVL RVLRLLRTLR PLRVISRAPG
1260 1270 1280 1290 1300
LKLVVETLIS SLKPIGNIVL ICCAFFIIFG ILGVQLFKGK FYHCLGVDTR
1310 1320 1330 1340 1350
NITNRSDCVA ANYRWVHHKY NFDNLGQALM SLFVLASKDG WVNIMYNGLD
1360 1370 1380 1390 1400
AVAVDQQPVT NHNPWMLLYF ISFLLIVSFF VLNMFVGVVV ENFHKCRQHQ
1410 1420 1430 1440 1450
EAEEARRREE KRLRRLEKKR RKAQRLPYYA TYCPTRLLIH SMCTSHYLDI
1460 1470 1480 1490 1500
FITFIICLNV VTMSLEHYNQ PTSLETALKY CNYMFTTVFV LEAVLKLVAF
1510 1520 1530 1540 1550
GLRRFFKDRW NQLDLAIVLL SVMGITLEEI EINAALPINP TIIRIMRVLR
1560 1570 1580 1590 1600
IARVLKLLKM ATGMRALLDT VVQALPQVGN LGLLFMLLFF IYAALGVELF
1610 1620 1630 1640 1650
GKLVCNDENP CEGMSRHATF ENFGMAFLTL FQVSTGDNWN GIMKDTLRDC
1660 1670 1680 1690 1700
THDERSCLSS LQFVSPLYFV SFVLTAQFVL INVVVAVLMK HLDDSNKEAQ
1710 1720 1730 1740 1750
EDAEMDAEIE LEMAHGLGPC PGPCPGPCPC PWPLAPVLAR GCPLVLLGAT
1760 1770 1780 1790 1800
GARIGRGRCW RRHRESPVPS TRYSPAQETL WLDSVSLIIK DSLEGELTII
1810 1820 1830 1840 1850
DNLSGSVFHH YASPDGCGKC HHDKQEVQLA ETEAFSLNSD RSSSILLGDD
1860 1870 1880 1890 1900
LSLEDPTACP PGRKDSKGEL DPPEPMRVGD LGECFFPLSS TAVSPDPENF
1910 1920 1930 1940 1950
LCEMEEIPFN PVQSWLKHDS SQAPPSPFSP DGSSPLLQMP AEFFHPAVSA
1960 1970 1980 1990 2000
SQKGPEKGTG TGTLPKIALQ GSWASLRSPS VNCTLLRQAT GSDTSLDASP
2010 2020 2030 2040 2050
SSSAGSLQTT LEDSLTLSDS PRRALGPPVQ VPGPRASLSP ATRRRLSLRG
2060 2070 2080 2090 2100
RGLFSLRGLR AHQRSHSSGG STSPGCTHHD SMDPSDEEGR GGAGGGGAGS
2110 2120 2130 2140 2150
EHSETLSSLS LTSLFCLPPT LPPPGLTPAR KFNSTSSLAA GPGRPGSTVS
2160 2170 2180 2190 2200
ARGLVRSPSW AADRSKDPPG QAQLVSGLGS SAPGPQPPPG ESTDAASKRK

R
Length:2,201
Mass (Da):243,693
Last modified:May 16, 2006 - v3
Checksum:i5791A4FDD7B882FE
GO
Isoform 2 (identifier: Q9Z0Y8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1732-1772: WPLAPVLARG...HRESPVPSTR → CPCPCPGPRL...DTESHLCRHC
     1827-1837: VQLAETEAFSL → TGLHPSCWGMT
     1838-2201: Missing.

Show »
Length:1,835
Mass (Da):205,342
Checksum:i1C6E482E29848F55
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti555P → L in AAD17796 (PubMed:10066244).Curated1
Sequence conflicti589E → K in AAD17796 (PubMed:10066244).Curated1
Sequence conflicti833Y → C in AAD17796 (PubMed:10066244).Curated1
Sequence conflicti1071C → R in AAD17796 (PubMed:10066244).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0183471732 – 1772WPLAP…VPSTR → CPCPCPGPRLPTSSPGAPGR GSGGAGAGGDTESHLCRHC in isoform 2. 1 PublicationAdd BLAST41
Alternative sequenceiVSP_0183481827 – 1837VQLAETEAFSL → TGLHPSCWGMT in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_0183491838 – 2201Missing in isoform 2. 1 PublicationAdd BLAST364

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086827 mRNA. Translation: AAD17796.2.
AF290214 mRNA. Translation: AAG35188.4.
RefSeqiNP_064469.3. NM_020084.3. [Q9Z0Y8-1]
UniGeneiRn.48680.

Genome annotation databases

GeneIDi56827.
KEGGirno:56827.
UCSCiRGD:68944. rat. [Q9Z0Y8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086827 mRNA. Translation: AAD17796.2.
AF290214 mRNA. Translation: AAG35188.4.
RefSeqiNP_064469.3. NM_020084.3. [Q9Z0Y8-1]
UniGeneiRn.48680.

3D structure databases

ProteinModelPortaliQ9Z0Y8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000046091.

Chemistry databases

GuidetoPHARMACOLOGYi537.

PTM databases

iPTMnetiQ9Z0Y8.
PhosphoSitePlusiQ9Z0Y8.

Proteomic databases

PaxDbiQ9Z0Y8.
PRIDEiQ9Z0Y8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi56827.
KEGGirno:56827.
UCSCiRGD:68944. rat. [Q9Z0Y8-1]

Organism-specific databases

CTDi8911.
RGDi68944. Cacna1i.

Phylogenomic databases

eggNOGiKOG2302. Eukaryota.
ENOG410XNP6. LUCA.
HOVERGENiHBG050764.
InParanoidiQ9Z0Y8.
KOiK04856.
PhylomeDBiQ9Z0Y8.

Miscellaneous databases

PROiQ9Z0Y8.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR030162. CACNA1I.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR005445. VDCC_T_a1.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF209. PTHR10037:SF209. 3 hits.
PfamiPF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01629. TVDCCALPHA1.
ProtoNetiSearch...

Entry informationi

Entry nameiCAC1I_RAT
AccessioniPrimary (citable) accession number: Q9Z0Y8
Secondary accession number(s): Q9EQ59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 16, 2006
Last modified: November 2, 2016
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.