Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Voltage-dependent T-type calcium channel subunit alpha-1I

Gene

Cacna1i

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This channel gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. Gates in voltage ranges similar to, but higher than alpha 1G or alpha 1H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei355 – 3551Calcium ion selectivity and permeabilityBy similarity
Sitei779 – 7791Calcium ion selectivity and permeabilityBy similarity
Sitei1339 – 13391Calcium ion selectivity and permeabilityBy similarity
Sitei1637 – 16371Calcium ion selectivity and permeabilityBy similarity

GO - Molecular functioni

  • low voltage-gated calcium channel activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent T-type calcium channel subunit alpha-1I
Alternative name(s):
CaVT.3
Voltage-gated calcium channel subunit alpha Cav3.3
Gene namesi
Name:Cacna1i
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68944. Cacna1i.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7676CytoplasmicSequence analysisAdd
BLAST
Transmembranei77 – 9721Helical; Name=S1 of repeat ISequence analysisAdd
BLAST
Topological domaini98 – 11518ExtracellularSequence analysisAdd
BLAST
Transmembranei116 – 13722Helical; Name=S2 of repeat ISequence analysisAdd
BLAST
Topological domaini138 – 1469CytoplasmicSequence analysis
Transmembranei147 – 16620Helical; Name=S3 of repeat ISequence analysisAdd
BLAST
Topological domaini167 – 1715ExtracellularSequence analysis
Transmembranei172 – 18918Helical; Name=S4 of repeat ISequence analysisAdd
BLAST
Topological domaini190 – 20920CytoplasmicSequence analysisAdd
BLAST
Transmembranei210 – 23021Helical; Name=S5 of repeat ISequence analysisAdd
BLAST
Topological domaini231 – 371141ExtracellularSequence analysisAdd
BLAST
Transmembranei372 – 39625Helical; Name=S6 of repeat ISequence analysisAdd
BLAST
Topological domaini397 – 598202CytoplasmicSequence analysisAdd
BLAST
Transmembranei599 – 61921Helical; Name=S1 of repeat IISequence analysisAdd
BLAST
Topological domaini620 – 63213ExtracellularSequence analysisAdd
BLAST
Transmembranei633 – 65422Helical; Name=S2 of repeat IISequence analysisAdd
BLAST
Topological domaini655 – 6606CytoplasmicSequence analysis
Transmembranei661 – 67919Helical; Name=S3 of repeat IISequence analysisAdd
BLAST
Topological domaini680 – 6878ExtracellularSequence analysis
Transmembranei688 – 71124Helical; Name=S4 of repeat IISequence analysisAdd
BLAST
Topological domaini712 – 72211CytoplasmicSequence analysisAdd
BLAST
Transmembranei723 – 74321Helical; Name=S5 of repeat IISequence analysisAdd
BLAST
Topological domaini744 – 79552ExtracellularSequence analysisAdd
BLAST
Transmembranei796 – 82025Helical; Name=S6 of repeat IISequence analysisAdd
BLAST
Topological domaini821 – 1125305CytoplasmicSequence analysisAdd
BLAST
Transmembranei1126 – 114823Helical; Name=S1 of repeat IIISequence analysisAdd
BLAST
Topological domaini1149 – 116618ExtracellularSequence analysisAdd
BLAST
Transmembranei1167 – 118721Helical; Name=S2 of repeat IIISequence analysisAdd
BLAST
Topological domaini1188 – 119710CytoplasmicSequence analysis
Transmembranei1198 – 121720Helical; Name=S3 of repeat IIISequence analysisAdd
BLAST
Topological domaini1218 – 123114ExtracellularSequence analysisAdd
BLAST
Transmembranei1232 – 125322Helical; Name=S4 of repeat IIISequence analysisAdd
BLAST
Topological domaini1254 – 126310CytoplasmicSequence analysis
Transmembranei1264 – 128724Helical; Name=S5 of repeat IIISequence analysisAdd
BLAST
Topological domaini1288 – 136477ExtracellularSequence analysisAdd
BLAST
Transmembranei1365 – 139026Helical; Name=S6 of repeat IIISequence analysisAdd
BLAST
Topological domaini1391 – 144555CytoplasmicSequence analysisAdd
BLAST
Transmembranei1446 – 146621Helical; Name=S1 of repeat IVSequence analysisAdd
BLAST
Topological domaini1467 – 148014ExtracellularSequence analysisAdd
BLAST
Transmembranei1481 – 150222Helical; Name=S2 of repeat IVSequence analysisAdd
BLAST
Topological domaini1503 – 15097CytoplasmicSequence analysis
Transmembranei1510 – 152819Helical; Name=S3 of repeat IVSequence analysisAdd
BLAST
Topological domaini1529 – 154214ExtracellularSequence analysisAdd
BLAST
Transmembranei1543 – 156624Helical; Name=S4 of repeat IVSequence analysisAdd
BLAST
Topological domaini1567 – 158014CytoplasmicSequence analysisAdd
BLAST
Transmembranei1581 – 160121Helical; Name=S5 of repeat IVSequence analysisAdd
BLAST
Topological domaini1602 – 166463ExtracellularSequence analysisAdd
BLAST
Transmembranei1665 – 169228Helical; Name=S6 of repeat IVSequence analysisAdd
BLAST
Topological domaini1693 – 1835143CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • intracellular Source: GOC
  • plasma membrane Source: GO_Central
  • voltage-gated calcium channel complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

GuidetoPHARMACOLOGYi537.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22012201Voltage-dependent T-type calcium channel subunit alpha-1IPRO_0000053958Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence analysis
Glycosylationi242 – 2421N-linked (GlcNAc...)Sequence analysis
Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence analysis
Modified residuei1017 – 10171PhosphoserineCombined sources
Glycosylationi1301 – 13011N-linked (GlcNAc...)Sequence analysis
Glycosylationi1304 – 13041N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

In response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9Z0Y8.
PRIDEiQ9Z0Y8.

PTM databases

iPTMnetiQ9Z0Y8.

Expressioni

Tissue specificityi

Brain.

Interactioni

Subunit structurei

Interacts with CATSPER1 and CATSPER2, leading to suppress T-type calcium channel activity.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000046091.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0Y8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati64 – 399336IAdd
BLAST
Repeati584 – 823240IIAdd
BLAST
Repeati1116 – 1393278IIIAdd
BLAST
Repeati1431 – 1692262IVAdd
BLAST

Domaini

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2302. Eukaryota.
ENOG410XNP6. LUCA.
HOVERGENiHBG050764.
InParanoidiQ9Z0Y8.
KOiK04856.
PhylomeDBiQ9Z0Y8.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR030162. CACNA1I.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR005445. VDCC_T_a1.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF209. PTHR10037:SF209. 3 hits.
PfamiPF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01629. TVDCCALPHA1.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z0Y8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADSNLPPSS AAAPAPEPGI TEQPGPRSPP PSPPGLEEPL EGTNPDVPHP
60 70 80 90 100
DLAPVAFFCL RQTTSPRNWC IKMVCNPWFE CVSMLVILLN CVTLGMYQPC
110 120 130 140 150
DDMECLSDRC KILQVFDDFI FIFFAMEMVL KMVALGIFGK KCYLGDTWNR
160 170 180 190 200
LDFFIVMAGM VEYSLDLQNI NLSAIRTVRV LRPLKAINRV PSMRILVNLL
210 220 230 240 250
LDTLPMLGNV LLLCFFVFFI FGIIGVQLWA GLLRNRCFLE ENFTIQGDVA
260 270 280 290 300
LPPYYQPEED DEMPFICSLT GDNGIMGCHE IPPLKEQGRE CCLSKDDVYD
310 320 330 340 350
FGAGRQDLNA SGLCVNWNRY YNVCRTGNAN PHKGAINFDN IGYAWIVIFQ
360 370 380 390 400
VITLEGWVEI MYYVMDAHSF YNFIYFILLI IVGSFFMINL CLVVIATQFS
410 420 430 440 450
ETKQREHRLM LEQRQRYLSS STVASYAEPG DCYEEIFQYV CHILRKAKRR
460 470 480 490 500
ALGLYQALQN RRQAMGPGTP APAKPGPHAK EPSHCKLCPR HSPLDPTPHT
510 520 530 540 550
LVQPISAILA SDPSSCPHCQ HEAGRRPSGL GSTDSGQEGS GSGGSAEAEA
560 570 580 590 600
NGDGPQSSED GVSSDLGKEE EQEDGAARLC GDVWRETREK LRGIVDSKYF
610 620 630 640 650
NRGIMMAILV NTVSMGIEHH EQPEELTNIL EICNVVFTSM FALEMILKLA
660 670 680 690 700
AFGLFDYLRN PYNIFDSIIV IISIWEIVGQ ADGGLSVLRT FRLLRVLKLV
710 720 730 740 750
RFMPALRRQL VVLMKTMDNV ATFCMLLMLF IFIFSILGMH IFGCKFSLRT
760 770 780 790 800
DTGDTVPDRK NFDSLLWAIV TVFQILTQED WNVVLYNGMA STTPWASLYF
810 820 830 840 850
VALMTFGNYV LFNLLVAILV EGFQAEGDAN RSYSDEDQSS SNLEEFDKLP
860 870 880 890 900
EGLDNSRDLK LCPIPMTPNG HLDPSLPLGA HLGPAGTMGT APRLSLQPDP
910 920 930 940 950
VLVALDSRKS SVMSLGRMSY DQRSLSSSRS SYYGPWGRSG TWASRRSSWN
960 970 980 990 1000
SLKHKPPSAE HESLLSGEGG GSCVRACEGA REEAPTRTAP LHAPHAHHAH
1010 1020 1030 1040 1050
HGPHLAHRHR HHRRTLSLDT RDSVDLGELV PVVGAHSRAA WRGAGQAPGH
1060 1070 1080 1090 1100
EDCNGRMPNI AKDVFTKMDD CRDRGEDEEE IDYTLCFRVR KMIDVYKPDW
1110 1120 1130 1140 1150
CEVREDWSVY LFSPENKFRI LCQTIIAHKL FDYVVLAFIF LNCITIALER
1160 1170 1180 1190 1200
PQIEAGSTER IFLTVSNYIF TAIFVGEMTL KVVSLGLYFG EQAYLRSSWN
1210 1220 1230 1240 1250
VLDGFLVFVS IIDIVVSVAS AGGAKILGVL RVLRLLRTLR PLRVISRAPG
1260 1270 1280 1290 1300
LKLVVETLIS SLKPIGNIVL ICCAFFIIFG ILGVQLFKGK FYHCLGVDTR
1310 1320 1330 1340 1350
NITNRSDCVA ANYRWVHHKY NFDNLGQALM SLFVLASKDG WVNIMYNGLD
1360 1370 1380 1390 1400
AVAVDQQPVT NHNPWMLLYF ISFLLIVSFF VLNMFVGVVV ENFHKCRQHQ
1410 1420 1430 1440 1450
EAEEARRREE KRLRRLEKKR RKAQRLPYYA TYCPTRLLIH SMCTSHYLDI
1460 1470 1480 1490 1500
FITFIICLNV VTMSLEHYNQ PTSLETALKY CNYMFTTVFV LEAVLKLVAF
1510 1520 1530 1540 1550
GLRRFFKDRW NQLDLAIVLL SVMGITLEEI EINAALPINP TIIRIMRVLR
1560 1570 1580 1590 1600
IARVLKLLKM ATGMRALLDT VVQALPQVGN LGLLFMLLFF IYAALGVELF
1610 1620 1630 1640 1650
GKLVCNDENP CEGMSRHATF ENFGMAFLTL FQVSTGDNWN GIMKDTLRDC
1660 1670 1680 1690 1700
THDERSCLSS LQFVSPLYFV SFVLTAQFVL INVVVAVLMK HLDDSNKEAQ
1710 1720 1730 1740 1750
EDAEMDAEIE LEMAHGLGPC PGPCPGPCPC PWPLAPVLAR GCPLVLLGAT
1760 1770 1780 1790 1800
GARIGRGRCW RRHRESPVPS TRYSPAQETL WLDSVSLIIK DSLEGELTII
1810 1820 1830 1840 1850
DNLSGSVFHH YASPDGCGKC HHDKQEVQLA ETEAFSLNSD RSSSILLGDD
1860 1870 1880 1890 1900
LSLEDPTACP PGRKDSKGEL DPPEPMRVGD LGECFFPLSS TAVSPDPENF
1910 1920 1930 1940 1950
LCEMEEIPFN PVQSWLKHDS SQAPPSPFSP DGSSPLLQMP AEFFHPAVSA
1960 1970 1980 1990 2000
SQKGPEKGTG TGTLPKIALQ GSWASLRSPS VNCTLLRQAT GSDTSLDASP
2010 2020 2030 2040 2050
SSSAGSLQTT LEDSLTLSDS PRRALGPPVQ VPGPRASLSP ATRRRLSLRG
2060 2070 2080 2090 2100
RGLFSLRGLR AHQRSHSSGG STSPGCTHHD SMDPSDEEGR GGAGGGGAGS
2110 2120 2130 2140 2150
EHSETLSSLS LTSLFCLPPT LPPPGLTPAR KFNSTSSLAA GPGRPGSTVS
2160 2170 2180 2190 2200
ARGLVRSPSW AADRSKDPPG QAQLVSGLGS SAPGPQPPPG ESTDAASKRK

R
Length:2,201
Mass (Da):243,693
Last modified:May 16, 2006 - v3
Checksum:i5791A4FDD7B882FE
GO
Isoform 2 (identifier: Q9Z0Y8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1732-1772: WPLAPVLARG...HRESPVPSTR → CPCPCPGPRL...DTESHLCRHC
     1827-1837: VQLAETEAFSL → TGLHPSCWGMT
     1838-2201: Missing.

Show »
Length:1,835
Mass (Da):205,342
Checksum:i1C6E482E29848F55
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti555 – 5551P → L in AAD17796 (PubMed:10066244).Curated
Sequence conflicti589 – 5891E → K in AAD17796 (PubMed:10066244).Curated
Sequence conflicti833 – 8331Y → C in AAD17796 (PubMed:10066244).Curated
Sequence conflicti1071 – 10711C → R in AAD17796 (PubMed:10066244).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1732 – 177241WPLAP…VPSTR → CPCPCPGPRLPTSSPGAPGR GSGGAGAGGDTESHLCRHC in isoform 2. 1 PublicationVSP_018347Add
BLAST
Alternative sequencei1827 – 183711VQLAETEAFSL → TGLHPSCWGMT in isoform 2. 1 PublicationVSP_018348Add
BLAST
Alternative sequencei1838 – 2201364Missing in isoform 2. 1 PublicationVSP_018349Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086827 mRNA. Translation: AAD17796.2.
AF290214 mRNA. Translation: AAG35188.4.
RefSeqiNP_064469.3. NM_020084.3. [Q9Z0Y8-1]
UniGeneiRn.48680.

Genome annotation databases

GeneIDi56827.
KEGGirno:56827.
UCSCiRGD:68944. rat. [Q9Z0Y8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086827 mRNA. Translation: AAD17796.2.
AF290214 mRNA. Translation: AAG35188.4.
RefSeqiNP_064469.3. NM_020084.3. [Q9Z0Y8-1]
UniGeneiRn.48680.

3D structure databases

ProteinModelPortaliQ9Z0Y8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000046091.

Chemistry

GuidetoPHARMACOLOGYi537.

PTM databases

iPTMnetiQ9Z0Y8.

Proteomic databases

PaxDbiQ9Z0Y8.
PRIDEiQ9Z0Y8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi56827.
KEGGirno:56827.
UCSCiRGD:68944. rat. [Q9Z0Y8-1]

Organism-specific databases

CTDi8911.
RGDi68944. Cacna1i.

Phylogenomic databases

eggNOGiKOG2302. Eukaryota.
ENOG410XNP6. LUCA.
HOVERGENiHBG050764.
InParanoidiQ9Z0Y8.
KOiK04856.
PhylomeDBiQ9Z0Y8.

Miscellaneous databases

PROiQ9Z0Y8.

Family and domain databases

Gene3Di1.20.120.350. 4 hits.
InterProiIPR030162. CACNA1I.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
IPR005445. VDCC_T_a1.
IPR002077. VDCCAlpha1.
[Graphical view]
PANTHERiPTHR10037:SF209. PTHR10037:SF209. 3 hits.
PfamiPF00520. Ion_trans. 4 hits.
[Graphical view]
PRINTSiPR00167. CACHANNEL.
PR01629. TVDCCALPHA1.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a novel member of the low voltage-activated T-type calcium channel family."
    Lee J.-H., Daud A.N., Cribbs L.L., Lacerda A.E., Pereverzev A., Kloeckner U., Schneider T., Perez-Reyes E.
    J. Neurosci. 19:1912-1921(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. Perez-Reyes E.
    Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 345; 1656 AND 1737.
  3. "Molecular and functional characterization of a family of rat brain T-type calcium channels."
    McRory J.E., Santi C.M., Hamming K.S.C., Mezeyova J., Sutton K.G., Baillie D.L., Stea A., Snutch T.P.
    J. Biol. Chem. 276:3999-4011(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. McRory J.E.
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  5. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1017, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCAC1I_RAT
AccessioniPrimary (citable) accession number: Q9Z0Y8
Secondary accession number(s): Q9EQ59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 16, 2006
Last modified: July 6, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.