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Protein

Phospholipase A2

Gene

Pla2g1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi50Calcium; via carbonyl oxygenBy similarity1
Metal bindingi52Calcium; via carbonyl oxygenBy similarity1
Metal bindingi54Calcium; via carbonyl oxygenBy similarity1
Active sitei70By similarity1
Metal bindingi71CalciumBy similarity1
Active sitei121By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipase A2 activity Source: MGI
  • calcium ion binding Source: MGI
  • phospholipase A2 activity Source: MGI
  • receptor binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482801. Acyl chain remodelling of PS.
R-MMU-1482839. Acyl chain remodelling of PE.
R-MMU-1482922. Acyl chain remodelling of PI.
R-MMU-1482925. Acyl chain remodelling of PG.
R-MMU-1483166. Synthesis of PA.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Group IB phospholipase A2
PLA2-Ib
Phosphatidylcholine 2-acylhydrolase 1B
Gene namesi
Name:Pla2g1b
Synonyms:Pla2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:101842. Pla2g1b.

Subcellular locationi

  • Secreted

  • Note: secreted from pancreatic acinar cells in its inactive form.By similarity

GO - Cellular componenti

  • cell surface Source: MGI
  • extracellular region Source: MGI
  • extracellular space Source: MGI
  • secretory granule Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4378.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15By similarityAdd BLAST15
PropeptideiPRO_000002274116 – 22Activation peptideBy similarity7
ChainiPRO_000002274223 – 146Phospholipase A2Add BLAST124

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi33 ↔ 99By similarity
Disulfide bondi49 ↔ 146By similarity
Disulfide bondi51 ↔ 67By similarity
Disulfide bondi66 ↔ 127By similarity
Disulfide bondi73 ↔ 120By similarity
Disulfide bondi83 ↔ 113By similarity
Disulfide bondi106 ↔ 118By similarity

Post-translational modificationi

Activated by trypsin cleavage in the duodenum. Can also be activated by thrombin or autocatalytically (By similarity).By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Zymogen

Proteomic databases

MaxQBiQ9Z0Y2.
PaxDbiQ9Z0Y2.
PRIDEiQ9Z0Y2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029522.
CleanExiMM_PLA2G1B.
ExpressionAtlasiQ9Z0Y2. baseline and differential.
GenevisibleiQ9Z0Y2. MM.

Interactioni

Subunit structurei

Monomer or homodimer. The inactive pro-form is a homotrimer (By similarity).By similarity

GO - Molecular functioni

  • receptor binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031495.

Chemistry databases

BindingDBiQ9Z0Y2.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0Y2.
SMRiQ9Z0Y2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4087. Eukaryota.
ENOG411283D. LUCA.
GeneTreeiENSGT00760000119160.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiQ9Z0Y2.
KOiK01047.
OMAiWQFRKMI.
OrthoDBiEOG091G0UZ3.
PhylomeDBiQ9Z0Y2.
TreeFamiTF319283.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0Y2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLLLAALL TAGAAAHSIS PRAVWQFRNM IKCTIPGSDP LKDYNNYGCY
60 70 80 90 100
CGLGGWGTPV DDLDRCCQTH DHCYSQAKKL ESCKFLIDNP YTNTYSYSCS
110 120 130 140
GSEITCSAKN NKCEDFICNC DREAAICFSK VPYNKEYKNL DTGKFC
Length:146
Mass (Da):16,290
Last modified:May 1, 1999 - v1
Checksum:i59500C68845B7C81
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52G → S in BAB26212 (PubMed:16141072).Curated1
Sequence conflicti58T → P in BAB26212 (PubMed:16141072).Curated1
Sequence conflicti78 – 79KK → EN in BAB26212 (PubMed:16141072).Curated2
Sequence conflicti87I → R in BAB26212 (PubMed:16141072).Curated1
Sequence conflicti95Y → F in BAB26212 (PubMed:16141072).Curated1
Sequence conflicti102S → G in BAB26212 (PubMed:16141072).Curated1
Sequence conflicti122R → S in BAB25608 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF094611 Genomic DNA. Translation: AAF02298.1.
AF097637 mRNA. Translation: AAD19896.1.
AF162712 mRNA. Translation: AAD45806.1.
AF187852 mRNA. Translation: AAF44297.1.
AF094610 mRNA. Translation: AAG27064.1.
AK028104 mRNA. Translation: BAC25749.1.
AK028134 mRNA. Translation: BAC25763.1.
AK008936 mRNA. Translation: BAB25978.1.
AK007730 mRNA. Translation: BAB25218.1.
AK007797 mRNA. Translation: BAB25263.1.
AK008664 mRNA. Translation: BAB25819.1.
AK008668 mRNA. Translation: BAB25822.1.
AK008841 mRNA. Translation: BAB25922.1.
AK008934 mRNA. Translation: BAB25976.1.
AK008331 mRNA. Translation: BAB25608.1.
AK009314 mRNA. Translation: BAB26212.1.
BC145908 mRNA. Translation: AAI45909.1.
BC145910 mRNA. Translation: AAI45911.1.
CCDSiCCDS19592.1.
RefSeqiNP_035237.1. NM_011107.1.
UniGeneiMm.20190.

Genome annotation databases

EnsembliENSMUST00000031495; ENSMUSP00000031495; ENSMUSG00000029522.
GeneIDi18778.
KEGGimmu:18778.
UCSCiuc008zdx.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF094611 Genomic DNA. Translation: AAF02298.1.
AF097637 mRNA. Translation: AAD19896.1.
AF162712 mRNA. Translation: AAD45806.1.
AF187852 mRNA. Translation: AAF44297.1.
AF094610 mRNA. Translation: AAG27064.1.
AK028104 mRNA. Translation: BAC25749.1.
AK028134 mRNA. Translation: BAC25763.1.
AK008936 mRNA. Translation: BAB25978.1.
AK007730 mRNA. Translation: BAB25218.1.
AK007797 mRNA. Translation: BAB25263.1.
AK008664 mRNA. Translation: BAB25819.1.
AK008668 mRNA. Translation: BAB25822.1.
AK008841 mRNA. Translation: BAB25922.1.
AK008934 mRNA. Translation: BAB25976.1.
AK008331 mRNA. Translation: BAB25608.1.
AK009314 mRNA. Translation: BAB26212.1.
BC145908 mRNA. Translation: AAI45909.1.
BC145910 mRNA. Translation: AAI45911.1.
CCDSiCCDS19592.1.
RefSeqiNP_035237.1. NM_011107.1.
UniGeneiMm.20190.

3D structure databases

ProteinModelPortaliQ9Z0Y2.
SMRiQ9Z0Y2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031495.

Chemistry databases

BindingDBiQ9Z0Y2.
ChEMBLiCHEMBL4378.

Proteomic databases

MaxQBiQ9Z0Y2.
PaxDbiQ9Z0Y2.
PRIDEiQ9Z0Y2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031495; ENSMUSP00000031495; ENSMUSG00000029522.
GeneIDi18778.
KEGGimmu:18778.
UCSCiuc008zdx.1. mouse.

Organism-specific databases

CTDi5319.
MGIiMGI:101842. Pla2g1b.

Phylogenomic databases

eggNOGiKOG4087. Eukaryota.
ENOG411283D. LUCA.
GeneTreeiENSGT00760000119160.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiQ9Z0Y2.
KOiK01047.
OMAiWQFRKMI.
OrthoDBiEOG091G0UZ3.
PhylomeDBiQ9Z0Y2.
TreeFamiTF319283.

Enzyme and pathway databases

ReactomeiR-MMU-1482788. Acyl chain remodelling of PC.
R-MMU-1482801. Acyl chain remodelling of PS.
R-MMU-1482839. Acyl chain remodelling of PE.
R-MMU-1482922. Acyl chain remodelling of PI.
R-MMU-1482925. Acyl chain remodelling of PG.
R-MMU-1483166. Synthesis of PA.

Miscellaneous databases

ChiTaRSiPla2g1b. mouse.
PROiQ9Z0Y2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029522.
CleanExiMM_PLA2G1B.
ExpressionAtlasiQ9Z0Y2. baseline and differential.
GenevisibleiQ9Z0Y2. MM.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA21B_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0Y2
Secondary accession number(s): A6H6K5, Q9D7E2, Q9D884
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.