Reviewed, UniProtKB/Swiss-Prot Q9Z0X4 (PDE3A_MOUSE)

Names and origin

Protein names

Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase A
    EC=3.1.4.17
Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase A
      Short name=CGI-PDE A

Gene names

Name:

Pde3a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1141 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Involved in oocyte maturation.
Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Enzyme regulation	Inhibited by cGMP By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.
Biophysicochemical properties	Kinetic parameters: KM=0.2 µM for cAMP

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	Metal-binding cAMP cGMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oocyte maturation Ref.1 Inferred from direct assay. Source: MGI regulation of meiosis Ref.1 Inferred from direct assay. Source: MGI response to drug Ref.1 Inferred from direct assay. Source: MGI signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell insoluble fraction Ref.1 Inferred from direct assay. Source: MGI soluble fraction Ref.1 Inferred from direct assay. Source: MGI
Molecular function	3',5'-cyclic-AMP phosphodiesterase activity Ref.1 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1141	1141	cGMP-inhibited 3',5'-cyclic phosphodiesterase A		PRO_0000198800
Regions
	Transmembrane	62 – 82	21	Potential
	Transmembrane	127 – 147	21	Potential
	Transmembrane	157 – 177	21	Potential
	Transmembrane	182 – 202	21	Potential
	Transmembrane	207 – 227	21	Potential
	Transmembrane	229 – 249	21	Potential
	Region	728 – 1086	359	Catalytic By similarity
Sites
	Active site	752	1	Proton donor By similarity
	Metal binding	756	1	Divalent metal cation 1 By similarity
	Metal binding	836	1	Divalent metal cation 1 By similarity
	Metal binding	837	1	Divalent metal cation 1 By similarity
	Metal binding	837	1	Divalent metal cation 2 By similarity
	Metal binding	950	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	310	1	Phosphoserine Ref.3
	Modified residue	425	1	Phosphoserine By similarity
	Modified residue	437	1	Phosphothreonine By similarity
	Modified residue	495	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9Z0X4-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3D9ACCFF928219D8	FASTA	1,141	124,513
	10 20 30 40 50 60 MAVRGEAAQD LAKPGLGGAS PARVARGNHR HRGESSPSPR GSGCCWRALA LQPLRRSPQL 70 80 90 100 110 120 SSALCAGSLS VLLALLVRLV GGEVGGELEK SQEAAAEEEE EEGARGGVFP GPRGGAPGGG 130 140 150 160 170 180 AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA GVRLPLAVAL LAACCAGEAL VQLSLGVGDG 190 200 210 220 230 240 RLLSLPAAGV LLSCLGGATW LVLRLRLGVL MVAWTSVLRT VALVSLERFK VAWRPYLAYL 250 260 270 280 290 300 AAVLGLLLAR YAEQILPQCS GPAPPRERFG SQLSARTKEE IPGWKRRRRS SSVVAGEMSG 310 320 330 340 350 360 CSGKSHRRTS LPCIPREQLM GHSEWDHKRG PRGSQSGTSI TVDIAVMGEA HGLITDLLAD 370 380 390 400 410 420 PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE NYTCSDSEEG LEKDKQAISK 430 440 450 460 470 480 RLRRSLPPGL LRRVSSTWTT TTSATGLPTL EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN 490 500 510 520 530 540 APGLTTLTKS RSFTSSYAVS AANHVKAKKQ NRPGGLAKIS PVPSPSSSPP QGSPASSPVS 550 560 570 580 590 600 NSASQQFPES PEVTIKRGPG SHRALTYTQS APDLSPQIPP PSVICSSCGR PYSQGNPADG 610 620 630 640 650 660 PSERSGPAML KPNRTDDTSQ VTSDYETNNN SDSSDILQNE EEAECQREPQ RKASACGTYT 670 680 690 700 710 720 SQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLM ENIGRKCGRI LSQVSYRLFE 730 740 750 760 770 780 DMGLFEAFKI PVREFMNYFH ALEIGYRDIP YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG 790 800 810 820 830 840 DHGSASDSDS DSGFTHGHMG YVFSKMYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH 850 860 870 880 890 900 PGRTNAFLVA TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL 910 920 930 940 950 960 VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD INGPAKCKEL 970 980 990 1000 1010 1020 HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL QESFISHIVG PLCHSYDSAG 1030 1040 1050 1060 1070 1080 LMPGKWVDDS DDSGDTDDPE EEEEEAETPH EDEACESSIA PRKKSFKRRR IYCQITQHLL 1090 1100 1110 1120 1130 1140 QNHMMWKKVI EEEQCLSGTE NQSLDQVPLQ HPSEQIQAIK EEEEEKGKPR AEETLAPQPD L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the cyclic guanosine monophosphate-inhibited phosphodiesterase PDE3A expressed in mouse oocyte." Shitsukawa K., Andersen C.B., Richard F.J., Horner A.K., Wiersma A., van Duin M., Conti M. Biol. Reprod. 65:188-196(2001) [PubMed: 11420239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: C57BL/6J. Tissue: Ovary.
[2]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-495, MASS SPECTROMETRY. Tissue: Liver.
[3]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-310, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF099187 mRNA. Translation: AAD16300.1.
IPI	IPI00129586.
RefSeq	NP_061249.1.
UniGene	Mm.103728
3D structure databases
SMR	Q9Z0X4. Positions 677-1087.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Z0X4.
PTM databases
PhosphoSite	Q9Z0X4.
Proteomic databases
PRIDE	Q9Z0X4.
Genome annotation databases
Ensembl	ENSMUST00000043259; ENSMUSP00000038749; ENSMUSG00000041741; Mus musculus. [Genome view]
GeneID	54611.
KEGG	mmu:54611.
UCSC	uc009eoo.1. mouse.
Organism-specific databases
CTD	54611.
MGI	MGI:1860764. Pde3a.
Phylogenomic databases
HOGENOM	HBG712991.
HOVERGEN	Q9Z0X4.
InParanoid	Q9Z0X4.
OMA	YSQGNPA.
OrthoDB	EOG91NX56.
PhylomeDB	Q9Z0X4.
Enzyme and pathway databases
BRENDA	3.1.4.17. 244.
Gene expression databases
ArrayExpress	Q9Z0X4.
Bgee	Q9Z0X4.
CleanEx	MM_PDE3A.
Genevestigator	Q9Z0X4.
Family and domain databases
InterPro	IPR003607. Metal-dep_PHydrolase_HD_dom. IPR002073. PDEase. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	311416.
SOURCE	Search...

Entry information

Entry name

PDE3A_MOUSE

Accession

Primary (citable) accession number: Q9Z0X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 1, 1999
Last modified:	January 19, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)