Reviewed, UniProtKB/Swiss-Prot Q9Z0X4 (PDE3A_MOUSE)

Names and origin

Protein names

Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase A
    EC=3.1.4.17
Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase A
      Short name=CGI-PDE A

Gene names

Name:

Pde3a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes both cyclic AMP (cAMP) and cyclic GMP (cGMP) By similarity.
Catalytic activity	Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.
Enzyme regulation	Inhibited by cGMP By similarity.
Subcellular location	Membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	cGMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oocyte maturation Inferred from direct assay. Source: MGI regulation of meiosis Inferred from direct assay. Source: MGI response to drug Inferred from direct assay. Source: MGI signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	insoluble fraction Inferred from direct assay. Source: MGI membrane Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Inferred from direct assay. Source: MGI
Molecular function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1141	1141	cGMP-inhibited 3',5'-cyclic phosphodiesterase A		PRO_0000198800
Amino acid modifications
	Modified residue	310	1	Phosphoserine By similarity
	Modified residue	425	1	Phosphoserine By similarity
	Modified residue	437	1	Phosphothreonine By similarity
	Modified residue	495	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9Z0X4-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3D9ACCFF928219D8	FASTA	1,141	124,513
	10 20 30 40 50 60 MAVRGEAAQD LAKPGLGGAS PARVARGNHR HRGESSPSPR GSGCCWRALA LQPLRRSPQL 70 80 90 100 110 120 SSALCAGSLS VLLALLVRLV GGEVGGELEK SQEAAAEEEE EEGARGGVFP GPRGGAPGGG 130 140 150 160 170 180 AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA GVRLPLAVAL LAACCAGEAL VQLSLGVGDG 190 200 210 220 230 240 RLLSLPAAGV LLSCLGGATW LVLRLRLGVL MVAWTSVLRT VALVSLERFK VAWRPYLAYL 250 260 270 280 290 300 AAVLGLLLAR YAEQILPQCS GPAPPRERFG SQLSARTKEE IPGWKRRRRS SSVVAGEMSG 310 320 330 340 350 360 CSGKSHRRTS LPCIPREQLM GHSEWDHKRG PRGSQSGTSI TVDIAVMGEA HGLITDLLAD 370 380 390 400 410 420 PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE NYTCSDSEEG LEKDKQAISK 430 440 450 460 470 480 RLRRSLPPGL LRRVSSTWTT TTSATGLPTL EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN 490 500 510 520 530 540 APGLTTLTKS RSFTSSYAVS AANHVKAKKQ NRPGGLAKIS PVPSPSSSPP QGSPASSPVS 550 560 570 580 590 600 NSASQQFPES PEVTIKRGPG SHRALTYTQS APDLSPQIPP PSVICSSCGR PYSQGNPADG 610 620 630 640 650 660 PSERSGPAML KPNRTDDTSQ VTSDYETNNN SDSSDILQNE EEAECQREPQ RKASACGTYT 670 680 690 700 710 720 SQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLM ENIGRKCGRI LSQVSYRLFE 730 740 750 760 770 780 DMGLFEAFKI PVREFMNYFH ALEIGYRDIP YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG 790 800 810 820 830 840 DHGSASDSDS DSGFTHGHMG YVFSKMYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH 850 860 870 880 890 900 PGRTNAFLVA TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL 910 920 930 940 950 960 VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD INGPAKCKEL 970 980 990 1000 1010 1020 HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL QESFISHIVG PLCHSYDSAG 1030 1040 1050 1060 1070 1080 LMPGKWVDDS DDSGDTDDPE EEEEEAETPH EDEACESSIA PRKKSFKRRR IYCQITQHLL 1090 1100 1110 1120 1130 1140 QNHMMWKKVI EEEQCLSGTE NQSLDQVPLQ HPSEQIQAIK EEEEEKGKPR AEETLAPQPD L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of cGMP-inhibited phosphodiesterase (PDE3A) expressed in mouse oocyte." Shitsukawa K., Andersen C.B., Richard F.J., Conti M. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Ovary.
[2]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-495, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF099187 mRNA. Translation: AAD16300.1.
IPI	IPI00129586.
RefSeq	NP_061249.1.
UniGene	Mm.103728 Mm.393561
3D structure databases
HSSP	HSSP built from PDB template 1MKD based on UniProtKB Q08499.
SMR	Q9Z0X4. Positions 677-1049.
ModBase	Search...
PTM databases
PhosphoSite	Q9Z0X4.
Proteomic databases
PRIDE	Q9Z0X4.
Genome annotation databases
Ensembl	ENSMUSG00000041741. Mus musculus. [Contig view]
GeneID	54611.
KEGG	mmu:54611.
Organism-specific databases
MGI	MGI:1860764. Pde3a.
Phylogenomic databases
HOGENOM	Q9Z0X4.
HOVERGEN	Q9Z0X4.
OMA	Q9Z0X4. DSGFTHG.
Enzyme and pathway databases
BRENDA	3.1.4.17. 244.
Gene expression databases
ArrayExpress	Q9Z0X4.
Bgee	Q9Z0X4.
CleanEx	MM_PDE3A.
Family and domain databases
InterPro	IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	311416.
SOURCE	Search...

Entry information

Entry name

PDE3A_MOUSE

Accession

Primary (citable) accession number: Q9Z0X4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)