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Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Gene

Pde3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Involved in oocyte maturation.1 Publication

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by cGMP.By similarity

Kineticsi

  1. KM=0.2 µM for cAMP1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei752 – 7521Proton donorBy similarity
    Metal bindingi756 – 7561Divalent metal cation 1By similarity
    Metal bindingi836 – 8361Divalent metal cation 1By similarity
    Metal bindingi837 – 8371Divalent metal cation 1By similarity
    Metal bindingi837 – 8371Divalent metal cation 2By similarity
    Metal bindingi950 – 9501Divalent metal cation 1By similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_311531. cGMP effects.
    REACT_313192. G alpha (s) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase A (EC:3.1.4.17)
    Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase A
    Short name:
    CGI-PDE A
    Gene namesi
    Name:Pde3a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 6

    Organism-specific databases

    MGIiMGI:1860764. Pde3a.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei62 – 8221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei127 – 14721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei157 – 17721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei182 – 20221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei207 – 22721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei229 – 24921HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • cytosol Source: MGI
    • integral component of membrane Source: UniProtKB-KW
    • membrane Source: MGI
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11411141cGMP-inhibited 3',5'-cyclic phosphodiesterase APRO_0000198800Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei310 – 3101PhosphoserineBy similarity
    Modified residuei520 – 5201PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9Z0X4.
    PaxDbiQ9Z0X4.
    PRIDEiQ9Z0X4.

    PTM databases

    PhosphoSiteiQ9Z0X4.

    Expressioni

    Gene expression databases

    BgeeiQ9Z0X4.
    CleanExiMM_PDE3A.
    ExpressionAtlasiQ9Z0X4. baseline and differential.
    GenevestigatoriQ9Z0X4.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9Z0X4. 3 interactions.
    MINTiMINT-4049569.
    STRINGi10090.ENSMUSP00000038749.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z0X4.
    SMRiQ9Z0X4. Positions 677-1087.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni728 – 1086359CatalyticBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG145074.
    GeneTreeiENSGT00760000119066.
    HOGENOMiHOG000060144.
    HOVERGENiHBG053541.
    InParanoidiQ9Z0X4.
    KOiK19021.
    OMAiTYAPETM.
    OrthoDBiEOG7KDF95.
    PhylomeDBiQ9Z0X4.
    TreeFamiTF329631.

    Family and domain databases

    Gene3Di1.10.1300.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z0X4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAVRGEAAQD LAKPGLGGAS PARVARGNHR HRGESSPSPR GSGCCWRALA
    60 70 80 90 100
    LQPLRRSPQL SSALCAGSLS VLLALLVRLV GGEVGGELEK SQEAAAEEEE
    110 120 130 140 150
    EEGARGGVFP GPRGGAPGGG AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA
    160 170 180 190 200
    GVRLPLAVAL LAACCAGEAL VQLSLGVGDG RLLSLPAAGV LLSCLGGATW
    210 220 230 240 250
    LVLRLRLGVL MVAWTSVLRT VALVSLERFK VAWRPYLAYL AAVLGLLLAR
    260 270 280 290 300
    YAEQILPQCS GPAPPRERFG SQLSARTKEE IPGWKRRRRS SSVVAGEMSG
    310 320 330 340 350
    CSGKSHRRTS LPCIPREQLM GHSEWDHKRG PRGSQSGTSI TVDIAVMGEA
    360 370 380 390 400
    HGLITDLLAD PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE
    410 420 430 440 450
    NYTCSDSEEG LEKDKQAISK RLRRSLPPGL LRRVSSTWTT TTSATGLPTL
    460 470 480 490 500
    EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN APGLTTLTKS RSFTSSYAVS
    510 520 530 540 550
    AANHVKAKKQ NRPGGLAKIS PVPSPSSSPP QGSPASSPVS NSASQQFPES
    560 570 580 590 600
    PEVTIKRGPG SHRALTYTQS APDLSPQIPP PSVICSSCGR PYSQGNPADG
    610 620 630 640 650
    PSERSGPAML KPNRTDDTSQ VTSDYETNNN SDSSDILQNE EEAECQREPQ
    660 670 680 690 700
    RKASACGTYT SQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLM
    710 720 730 740 750
    ENIGRKCGRI LSQVSYRLFE DMGLFEAFKI PVREFMNYFH ALEIGYRDIP
    760 770 780 790 800
    YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG DHGSASDSDS DSGFTHGHMG
    810 820 830 840 850
    YVFSKMYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH PGRTNAFLVA
    860 870 880 890 900
    TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL
    910 920 930 940 950
    VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD
    960 970 980 990 1000
    INGPAKCKEL HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL
    1010 1020 1030 1040 1050
    QESFISHIVG PLCHSYDSAG LMPGKWVDDS DDSGDTDDPE EEEEEAETPH
    1060 1070 1080 1090 1100
    EDEACESSIA PRKKSFKRRR IYCQITQHLL QNHMMWKKVI EEEQCLSGTE
    1110 1120 1130 1140
    NQSLDQVPLQ HPSEQIQAIK EEEEEKGKPR AEETLAPQPD L
    Length:1,141
    Mass (Da):124,513
    Last modified:May 1, 1999 - v1
    Checksum:i3D9ACCFF928219D8
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF099187 mRNA. Translation: AAD16300.1.
    CCDSiCCDS20676.1.
    RefSeqiNP_061249.1. NM_018779.1.
    UniGeneiMm.103728.
    Mm.485878.

    Genome annotation databases

    EnsembliENSMUST00000043259; ENSMUSP00000038749; ENSMUSG00000041741.
    GeneIDi54611.
    KEGGimmu:54611.
    UCSCiuc009eon.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF099187 mRNA. Translation: AAD16300.1.
    CCDSiCCDS20676.1.
    RefSeqiNP_061249.1. NM_018779.1.
    UniGeneiMm.103728.
    Mm.485878.

    3D structure databases

    ProteinModelPortaliQ9Z0X4.
    SMRiQ9Z0X4. Positions 677-1087.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    IntActiQ9Z0X4. 3 interactions.
    MINTiMINT-4049569.
    STRINGi10090.ENSMUSP00000038749.

    PTM databases

    PhosphoSiteiQ9Z0X4.

    Proteomic databases

    MaxQBiQ9Z0X4.
    PaxDbiQ9Z0X4.
    PRIDEiQ9Z0X4.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000043259; ENSMUSP00000038749; ENSMUSG00000041741.
    GeneIDi54611.
    KEGGimmu:54611.
    UCSCiuc009eon.1. mouse.

    Organism-specific databases

    CTDi5139.
    MGIiMGI:1860764. Pde3a.

    Phylogenomic databases

    eggNOGiNOG145074.
    GeneTreeiENSGT00760000119066.
    HOGENOMiHOG000060144.
    HOVERGENiHBG053541.
    InParanoidiQ9Z0X4.
    KOiK19021.
    OMAiTYAPETM.
    OrthoDBiEOG7KDF95.
    PhylomeDBiQ9Z0X4.
    TreeFamiTF329631.

    Enzyme and pathway databases

    ReactomeiREACT_311531. cGMP effects.
    REACT_313192. G alpha (s) signalling events.

    Miscellaneous databases

    ChiTaRSiPde3a. mouse.
    NextBioi311416.
    PROiQ9Z0X4.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ9Z0X4.
    CleanExiMM_PDE3A.
    ExpressionAtlasiQ9Z0X4. baseline and differential.
    GenevestigatoriQ9Z0X4.

    Family and domain databases

    Gene3Di1.10.1300.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Cloning and characterization of the cyclic guanosine monophosphate-inhibited phosphodiesterase PDE3A expressed in mouse oocyte."
      Shitsukawa K., Andersen C.B., Richard F.J., Horner A.K., Wiersma A., van Duin M., Conti M.
      Biol. Reprod. 65:188-196(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: C57BL/6J.
      Tissue: Ovary.

    Entry informationi

    Entry nameiPDE3A_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z0X4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 1, 1999
    Last modified: May 27, 2015
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.