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Q9Z0X4

- PDE3A_MOUSE

UniProt

Q9Z0X4 - PDE3A_MOUSE

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Protein
cGMP-inhibited 3',5'-cyclic phosphodiesterase A
Gene
Pde3a
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Involved in oocyte maturation.1 Publication

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulationi

Inhibited by cGMP By similarity.

Kineticsi

  1. KM=0.2 µM for cAMP1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei752 – 7521Proton donor By similarity
Metal bindingi756 – 7561Divalent metal cation 1 By similarity
Metal bindingi836 – 8361Divalent metal cation 1 By similarity
Metal bindingi837 – 8371Divalent metal cation 1 By similarity
Metal bindingi837 – 8371Divalent metal cation 2 By similarity
Metal bindingi950 – 9501Divalent metal cation 1 By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
  2. cAMP binding Source: Ensembl
  3. cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Source: Ensembl
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cAMP catabolic process Source: Ensembl
  2. cAMP-mediated signaling Source: Ensembl
  3. cGMP-mediated signaling Source: Ensembl
  4. cellular response to cGMP Source: Ensembl
  5. cellular response to transforming growth factor beta stimulus Source: Ensembl
  6. diterpenoid metabolic process Source: Ensembl
  7. negative regulation of apoptotic process Source: Ensembl
  8. negative regulation of vascular permeability Source: Ensembl
  9. oocyte maturation Source: MGI
  10. positive regulation of oocyte development Source: MGI
  11. positive regulation of vascular permeability Source: Ensembl
  12. regulation of meiosis Source: MGI
  13. response to cAMP Source: Ensembl
  14. response to drug Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, cGMP, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-inhibited 3',5'-cyclic phosphodiesterase A (EC:3.1.4.17)
Alternative name(s):
Cyclic GMP-inhibited phosphodiesterase A
Short name:
CGI-PDE A
Gene namesi
Name:Pde3a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1860764. Pde3a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei62 – 8221Helical; Reviewed prediction
Add
BLAST
Transmembranei127 – 14721Helical; Reviewed prediction
Add
BLAST
Transmembranei157 – 17721Helical; Reviewed prediction
Add
BLAST
Transmembranei182 – 20221Helical; Reviewed prediction
Add
BLAST
Transmembranei207 – 22721Helical; Reviewed prediction
Add
BLAST
Transmembranei229 – 24921Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11411141cGMP-inhibited 3',5'-cyclic phosphodiesterase A
PRO_0000198800Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei310 – 3101Phosphoserine By similarity
Modified residuei520 – 5201Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Z0X4.
PRIDEiQ9Z0X4.

PTM databases

PhosphoSiteiQ9Z0X4.

Expressioni

Gene expression databases

ArrayExpressiQ9Z0X4.
BgeeiQ9Z0X4.
CleanExiMM_PDE3A.
GenevestigatoriQ9Z0X4.

Interactioni

Protein-protein interaction databases

IntActiQ9Z0X4. 3 interactions.
MINTiMINT-4049569.
STRINGi10090.ENSMUSP00000038749.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0X4.
SMRiQ9Z0X4. Positions 677-1087.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni728 – 1086359Catalytic By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG145074.
GeneTreeiENSGT00750000117528.
HOGENOMiHOG000060144.
HOVERGENiHBG053541.
InParanoidiQ9Z0X4.
KOiK13296.
OMAiPETMMFL.
OrthoDBiEOG7KDF95.
PhylomeDBiQ9Z0X4.
TreeFamiTF329631.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z0X4-1 [UniParc]FASTAAdd to Basket

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MAVRGEAAQD LAKPGLGGAS PARVARGNHR HRGESSPSPR GSGCCWRALA     50
LQPLRRSPQL SSALCAGSLS VLLALLVRLV GGEVGGELEK SQEAAAEEEE 100
EEGARGGVFP GPRGGAPGGG AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA 150
GVRLPLAVAL LAACCAGEAL VQLSLGVGDG RLLSLPAAGV LLSCLGGATW 200
LVLRLRLGVL MVAWTSVLRT VALVSLERFK VAWRPYLAYL AAVLGLLLAR 250
YAEQILPQCS GPAPPRERFG SQLSARTKEE IPGWKRRRRS SSVVAGEMSG 300
CSGKSHRRTS LPCIPREQLM GHSEWDHKRG PRGSQSGTSI TVDIAVMGEA 350
HGLITDLLAD PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE 400
NYTCSDSEEG LEKDKQAISK RLRRSLPPGL LRRVSSTWTT TTSATGLPTL 450
EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN APGLTTLTKS RSFTSSYAVS 500
AANHVKAKKQ NRPGGLAKIS PVPSPSSSPP QGSPASSPVS NSASQQFPES 550
PEVTIKRGPG SHRALTYTQS APDLSPQIPP PSVICSSCGR PYSQGNPADG 600
PSERSGPAML KPNRTDDTSQ VTSDYETNNN SDSSDILQNE EEAECQREPQ 650
RKASACGTYT SQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLM 700
ENIGRKCGRI LSQVSYRLFE DMGLFEAFKI PVREFMNYFH ALEIGYRDIP 750
YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG DHGSASDSDS DSGFTHGHMG 800
YVFSKMYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH PGRTNAFLVA 850
TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL 900
VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD 950
INGPAKCKEL HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL 1000
QESFISHIVG PLCHSYDSAG LMPGKWVDDS DDSGDTDDPE EEEEEAETPH 1050
EDEACESSIA PRKKSFKRRR IYCQITQHLL QNHMMWKKVI EEEQCLSGTE 1100
NQSLDQVPLQ HPSEQIQAIK EEEEEKGKPR AEETLAPQPD L 1141
Length:1,141
Mass (Da):124,513
Last modified:May 1, 1999 - v1
Checksum:i3D9ACCFF928219D8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF099187 mRNA. Translation: AAD16300.1.
CCDSiCCDS20676.1.
RefSeqiNP_061249.1. NM_018779.1.
UniGeneiMm.103728.
Mm.485878.

Genome annotation databases

EnsembliENSMUST00000043259; ENSMUSP00000038749; ENSMUSG00000041741.
GeneIDi54611.
KEGGimmu:54611.
UCSCiuc009eon.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF099187 mRNA. Translation: AAD16300.1 .
CCDSi CCDS20676.1.
RefSeqi NP_061249.1. NM_018779.1.
UniGenei Mm.103728.
Mm.485878.

3D structure databases

ProteinModelPortali Q9Z0X4.
SMRi Q9Z0X4. Positions 677-1087.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9Z0X4. 3 interactions.
MINTi MINT-4049569.
STRINGi 10090.ENSMUSP00000038749.

PTM databases

PhosphoSitei Q9Z0X4.

Proteomic databases

PaxDbi Q9Z0X4.
PRIDEi Q9Z0X4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000043259 ; ENSMUSP00000038749 ; ENSMUSG00000041741 .
GeneIDi 54611.
KEGGi mmu:54611.
UCSCi uc009eon.1. mouse.

Organism-specific databases

CTDi 5139.
MGIi MGI:1860764. Pde3a.

Phylogenomic databases

eggNOGi NOG145074.
GeneTreei ENSGT00750000117528.
HOGENOMi HOG000060144.
HOVERGENi HBG053541.
InParanoidi Q9Z0X4.
KOi K13296.
OMAi PETMMFL.
OrthoDBi EOG7KDF95.
PhylomeDBi Q9Z0X4.
TreeFami TF329631.

Miscellaneous databases

NextBioi 311416.
PROi Q9Z0X4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Z0X4.
Bgeei Q9Z0X4.
CleanExi MM_PDE3A.
Genevestigatori Q9Z0X4.

Family and domain databases

Gene3Di 1.10.1300.10. 2 hits.
InterProi IPR003607. HD/PDEase_dom.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the cyclic guanosine monophosphate-inhibited phosphodiesterase PDE3A expressed in mouse oocyte."
    Shitsukawa K., Andersen C.B., Richard F.J., Horner A.K., Wiersma A., van Duin M., Conti M.
    Biol. Reprod. 65:188-196(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: C57BL/6J.
    Tissue: Ovary.

Entry informationi

Entry nameiPDE3A_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0X4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 1, 1999
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi