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Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Gene

Pde3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Involved in oocyte maturation.1 Publication

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by cGMP.By similarity

Kineticsi

  1. KM=0.2 µM for cAMP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei752Proton donorBy similarity1
    Metal bindingi756Divalent metal cation 1By similarity1
    Metal bindingi836Divalent metal cation 1By similarity1
    Metal bindingi837Divalent metal cation 1By similarity1
    Metal bindingi837Divalent metal cation 2By similarity1
    Metal bindingi950Divalent metal cation 1By similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    LigandcAMP, cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiR-MMU-418457 cGMP effects
    R-MMU-418555 G alpha (s) signalling events

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase A (EC:3.1.4.17)
    Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase A
    Short name:
    CGI-PDE A
    Gene namesi
    Name:Pde3a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 6

    Organism-specific databases

    MGIiMGI:1860764 Pde3a

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
    Transmembranei127 – 147HelicalSequence analysisAdd BLAST21
    Transmembranei157 – 177HelicalSequence analysisAdd BLAST21
    Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
    Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
    Transmembranei229 – 249HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001988001 – 1141cGMP-inhibited 3',5'-cyclic phosphodiesterase AAdd BLAST1141

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei310PhosphoserineBy similarity1
    Modified residuei492PhosphoserineCombined sources1
    Modified residuei520PhosphoserineCombined sources1
    Modified residuei524PhosphoserineCombined sources1
    Modified residuei533PhosphoserineCombined sources1
    Modified residuei1033PhosphoserineCombined sources1
    Modified residuei1036PhosphothreonineCombined sources1
    Cross-linki1120Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

    Keywords - PTMi

    Isopeptide bond, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ9Z0X4
    PaxDbiQ9Z0X4
    PRIDEiQ9Z0X4

    PTM databases

    iPTMnetiQ9Z0X4
    PhosphoSitePlusiQ9Z0X4

    Expressioni

    Gene expression databases

    BgeeiENSMUSG00000041741
    CleanExiMM_PDE3A
    ExpressionAtlasiQ9Z0X4 baseline and differential
    GenevisibleiQ9Z0X4 MM

    Interactioni

    Protein-protein interaction databases

    IntActiQ9Z0X4, 3 interactors
    MINTiQ9Z0X4
    STRINGi10090.ENSMUSP00000038749

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z0X4
    SMRiQ9Z0X4
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini674 – 1093PDEasePROSITE-ProRule annotationAdd BLAST420

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IEGG Eukaryota
    ENOG410XT2V LUCA
    GeneTreeiENSGT00760000119066
    HOGENOMiHOG000060144
    HOVERGENiHBG053541
    InParanoidiQ9Z0X4
    KOiK19021
    OMAiPNEEETC
    OrthoDBiEOG091G0BTI
    PhylomeDBiQ9Z0X4
    TreeFamiTF329631

    Family and domain databases

    CDDicd00077 HDc, 1 hit
    Gene3Di1.10.1300.10, 1 hit
    InterProiView protein in InterPro
    IPR003607 HD/PDEase_dom
    IPR002073 PDEase_catalytic_dom
    IPR036971 PDEase_catalytic_dom_sf
    IPR023174 PDEase_CS
    PfamiView protein in Pfam
    PF00233 PDEase_I, 1 hit
    SMARTiView protein in SMART
    SM00471 HDc, 1 hit
    PROSITEiView protein in PROSITE
    PS00126 PDEASE_I_1, 1 hit
    PS51845 PDEASE_I_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9Z0X4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAVRGEAAQD LAKPGLGGAS PARVARGNHR HRGESSPSPR GSGCCWRALA
    60 70 80 90 100
    LQPLRRSPQL SSALCAGSLS VLLALLVRLV GGEVGGELEK SQEAAAEEEE
    110 120 130 140 150
    EEGARGGVFP GPRGGAPGGG AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA
    160 170 180 190 200
    GVRLPLAVAL LAACCAGEAL VQLSLGVGDG RLLSLPAAGV LLSCLGGATW
    210 220 230 240 250
    LVLRLRLGVL MVAWTSVLRT VALVSLERFK VAWRPYLAYL AAVLGLLLAR
    260 270 280 290 300
    YAEQILPQCS GPAPPRERFG SQLSARTKEE IPGWKRRRRS SSVVAGEMSG
    310 320 330 340 350
    CSGKSHRRTS LPCIPREQLM GHSEWDHKRG PRGSQSGTSI TVDIAVMGEA
    360 370 380 390 400
    HGLITDLLAD PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE
    410 420 430 440 450
    NYTCSDSEEG LEKDKQAISK RLRRSLPPGL LRRVSSTWTT TTSATGLPTL
    460 470 480 490 500
    EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN APGLTTLTKS RSFTSSYAVS
    510 520 530 540 550
    AANHVKAKKQ NRPGGLAKIS PVPSPSSSPP QGSPASSPVS NSASQQFPES
    560 570 580 590 600
    PEVTIKRGPG SHRALTYTQS APDLSPQIPP PSVICSSCGR PYSQGNPADG
    610 620 630 640 650
    PSERSGPAML KPNRTDDTSQ VTSDYETNNN SDSSDILQNE EEAECQREPQ
    660 670 680 690 700
    RKASACGTYT SQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLM
    710 720 730 740 750
    ENIGRKCGRI LSQVSYRLFE DMGLFEAFKI PVREFMNYFH ALEIGYRDIP
    760 770 780 790 800
    YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG DHGSASDSDS DSGFTHGHMG
    810 820 830 840 850
    YVFSKMYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH PGRTNAFLVA
    860 870 880 890 900
    TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL
    910 920 930 940 950
    VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD
    960 970 980 990 1000
    INGPAKCKEL HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL
    1010 1020 1030 1040 1050
    QESFISHIVG PLCHSYDSAG LMPGKWVDDS DDSGDTDDPE EEEEEAETPH
    1060 1070 1080 1090 1100
    EDEACESSIA PRKKSFKRRR IYCQITQHLL QNHMMWKKVI EEEQCLSGTE
    1110 1120 1130 1140
    NQSLDQVPLQ HPSEQIQAIK EEEEEKGKPR AEETLAPQPD L
    Length:1,141
    Mass (Da):124,513
    Last modified:May 1, 1999 - v1
    Checksum:i3D9ACCFF928219D8
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF099187 mRNA Translation: AAD16300.1
    CCDSiCCDS20676.1
    RefSeqiNP_061249.1, NM_018779.1
    UniGeneiMm.103728
    Mm.485878

    Genome annotation databases

    EnsembliENSMUST00000043259; ENSMUSP00000038749; ENSMUSG00000041741
    GeneIDi54611
    KEGGimmu:54611
    UCSCiuc009eon.1 mouse

    Similar proteinsi

    Entry informationi

    Entry nameiPDE3A_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z0X4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 1, 1999
    Last modified: June 20, 2018
    This is version 135 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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