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Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Gene

Pde3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Involved in oocyte maturation.1 Publication

Catalytic activityi

Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by cGMP.By similarity

Kineticsi

  1. KM=0.2 µM for cAMP1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei752 – 7521Proton donorBy similarity
Metal bindingi756 – 7561Divalent metal cation 1By similarity
Metal bindingi836 – 8361Divalent metal cation 1By similarity
Metal bindingi837 – 8371Divalent metal cation 1By similarity
Metal bindingi837 – 8371Divalent metal cation 2By similarity
Metal bindingi950 – 9501Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
  2. cAMP binding Source: Ensembl
  3. cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Source: Ensembl
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cAMP catabolic process Source: MGI
  2. cAMP-mediated signaling Source: MGI
  3. cellular response to cGMP Source: MGI
  4. cellular response to transforming growth factor beta stimulus Source: Ensembl
  5. cGMP-mediated signaling Source: MGI
  6. diterpenoid metabolic process Source: Ensembl
  7. negative regulation of apoptotic process Source: Ensembl
  8. negative regulation of vascular permeability Source: MGI
  9. oocyte maturation Source: MGI
  10. positive regulation of oocyte development Source: MGI
  11. positive regulation of vascular permeability Source: MGI
  12. regulation of meiosis Source: MGI
  13. response to cAMP Source: Ensembl
  14. response to drug Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_311531. cGMP effects.
REACT_313192. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-inhibited 3',5'-cyclic phosphodiesterase A (EC:3.1.4.17)
Alternative name(s):
Cyclic GMP-inhibited phosphodiesterase A
Short name:
CGI-PDE A
Gene namesi
Name:Pde3a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1860764. Pde3a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei62 – 8221HelicalSequence AnalysisAdd
BLAST
Transmembranei127 – 14721HelicalSequence AnalysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence AnalysisAdd
BLAST
Transmembranei182 – 20221HelicalSequence AnalysisAdd
BLAST
Transmembranei207 – 22721HelicalSequence AnalysisAdd
BLAST
Transmembranei229 – 24921HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytosol Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11411141cGMP-inhibited 3',5'-cyclic phosphodiesterase APRO_0000198800Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei310 – 3101PhosphoserineBy similarity
Modified residuei520 – 5201PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z0X4.
PaxDbiQ9Z0X4.
PRIDEiQ9Z0X4.

PTM databases

PhosphoSiteiQ9Z0X4.

Expressioni

Gene expression databases

BgeeiQ9Z0X4.
CleanExiMM_PDE3A.
ExpressionAtlasiQ9Z0X4. baseline and differential.
GenevestigatoriQ9Z0X4.

Interactioni

Protein-protein interaction databases

IntActiQ9Z0X4. 3 interactions.
MINTiMINT-4049569.
STRINGi10090.ENSMUSP00000038749.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0X4.
SMRiQ9Z0X4. Positions 677-1087.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni728 – 1086359CatalyticBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG145074.
GeneTreeiENSGT00760000119066.
HOGENOMiHOG000060144.
HOVERGENiHBG053541.
InParanoidiQ9Z0X4.
KOiK19021.
OMAiTYAPETM.
OrthoDBiEOG7KDF95.
PhylomeDBiQ9Z0X4.
TreeFamiTF329631.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z0X4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVRGEAAQD LAKPGLGGAS PARVARGNHR HRGESSPSPR GSGCCWRALA
60 70 80 90 100
LQPLRRSPQL SSALCAGSLS VLLALLVRLV GGEVGGELEK SQEAAAEEEE
110 120 130 140 150
EEGARGGVFP GPRGGAPGGG AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA
160 170 180 190 200
GVRLPLAVAL LAACCAGEAL VQLSLGVGDG RLLSLPAAGV LLSCLGGATW
210 220 230 240 250
LVLRLRLGVL MVAWTSVLRT VALVSLERFK VAWRPYLAYL AAVLGLLLAR
260 270 280 290 300
YAEQILPQCS GPAPPRERFG SQLSARTKEE IPGWKRRRRS SSVVAGEMSG
310 320 330 340 350
CSGKSHRRTS LPCIPREQLM GHSEWDHKRG PRGSQSGTSI TVDIAVMGEA
360 370 380 390 400
HGLITDLLAD PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE
410 420 430 440 450
NYTCSDSEEG LEKDKQAISK RLRRSLPPGL LRRVSSTWTT TTSATGLPTL
460 470 480 490 500
EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN APGLTTLTKS RSFTSSYAVS
510 520 530 540 550
AANHVKAKKQ NRPGGLAKIS PVPSPSSSPP QGSPASSPVS NSASQQFPES
560 570 580 590 600
PEVTIKRGPG SHRALTYTQS APDLSPQIPP PSVICSSCGR PYSQGNPADG
610 620 630 640 650
PSERSGPAML KPNRTDDTSQ VTSDYETNNN SDSSDILQNE EEAECQREPQ
660 670 680 690 700
RKASACGTYT SQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLM
710 720 730 740 750
ENIGRKCGRI LSQVSYRLFE DMGLFEAFKI PVREFMNYFH ALEIGYRDIP
760 770 780 790 800
YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG DHGSASDSDS DSGFTHGHMG
810 820 830 840 850
YVFSKMYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH PGRTNAFLVA
860 870 880 890 900
TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL
910 920 930 940 950
VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD
960 970 980 990 1000
INGPAKCKEL HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL
1010 1020 1030 1040 1050
QESFISHIVG PLCHSYDSAG LMPGKWVDDS DDSGDTDDPE EEEEEAETPH
1060 1070 1080 1090 1100
EDEACESSIA PRKKSFKRRR IYCQITQHLL QNHMMWKKVI EEEQCLSGTE
1110 1120 1130 1140
NQSLDQVPLQ HPSEQIQAIK EEEEEKGKPR AEETLAPQPD L
Length:1,141
Mass (Da):124,513
Last modified:May 1, 1999 - v1
Checksum:i3D9ACCFF928219D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099187 mRNA. Translation: AAD16300.1.
CCDSiCCDS20676.1.
RefSeqiNP_061249.1. NM_018779.1.
UniGeneiMm.103728.
Mm.485878.

Genome annotation databases

EnsembliENSMUST00000043259; ENSMUSP00000038749; ENSMUSG00000041741.
GeneIDi54611.
KEGGimmu:54611.
UCSCiuc009eon.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099187 mRNA. Translation: AAD16300.1.
CCDSiCCDS20676.1.
RefSeqiNP_061249.1. NM_018779.1.
UniGeneiMm.103728.
Mm.485878.

3D structure databases

ProteinModelPortaliQ9Z0X4.
SMRiQ9Z0X4. Positions 677-1087.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z0X4. 3 interactions.
MINTiMINT-4049569.
STRINGi10090.ENSMUSP00000038749.

PTM databases

PhosphoSiteiQ9Z0X4.

Proteomic databases

MaxQBiQ9Z0X4.
PaxDbiQ9Z0X4.
PRIDEiQ9Z0X4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043259; ENSMUSP00000038749; ENSMUSG00000041741.
GeneIDi54611.
KEGGimmu:54611.
UCSCiuc009eon.1. mouse.

Organism-specific databases

CTDi5139.
MGIiMGI:1860764. Pde3a.

Phylogenomic databases

eggNOGiNOG145074.
GeneTreeiENSGT00760000119066.
HOGENOMiHOG000060144.
HOVERGENiHBG053541.
InParanoidiQ9Z0X4.
KOiK19021.
OMAiTYAPETM.
OrthoDBiEOG7KDF95.
PhylomeDBiQ9Z0X4.
TreeFamiTF329631.

Enzyme and pathway databases

ReactomeiREACT_311531. cGMP effects.
REACT_313192. G alpha (s) signalling events.

Miscellaneous databases

ChiTaRSiPde3a. mouse.
NextBioi311416.
PROiQ9Z0X4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z0X4.
CleanExiMM_PDE3A.
ExpressionAtlasiQ9Z0X4. baseline and differential.
GenevestigatoriQ9Z0X4.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of the cyclic guanosine monophosphate-inhibited phosphodiesterase PDE3A expressed in mouse oocyte."
    Shitsukawa K., Andersen C.B., Richard F.J., Horner A.K., Wiersma A., van Duin M., Conti M.
    Biol. Reprod. 65:188-196(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: C57BL/6J.
    Tissue: Ovary.

Entry informationi

Entry nameiPDE3A_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0X4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: May 1, 1999
Last modified: April 29, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.