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Q9Z0X4

- PDE3A_MOUSE

UniProt

Q9Z0X4 - PDE3A_MOUSE

Protein

cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Gene

Pde3a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Involved in oocyte maturation.1 Publication

    Catalytic activityi

    Nucleoside 3',5'-cyclic phosphate + H2O = nucleoside 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Enzyme regulationi

    Inhibited by cGMP.By similarity

    Kineticsi

    1. KM=0.2 µM for cAMP1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei752 – 7521Proton donorBy similarity
    Metal bindingi756 – 7561Divalent metal cation 1By similarity
    Metal bindingi836 – 8361Divalent metal cation 1By similarity
    Metal bindingi837 – 8371Divalent metal cation 1By similarity
    Metal bindingi837 – 8371Divalent metal cation 2By similarity
    Metal bindingi950 – 9501Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: MGI
    2. cAMP binding Source: Ensembl
    3. cGMP-inhibited cyclic-nucleotide phosphodiesterase activity Source: Ensembl
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cAMP catabolic process Source: Ensembl
    2. cAMP-mediated signaling Source: Ensembl
    3. cellular response to cGMP Source: Ensembl
    4. cellular response to transforming growth factor beta stimulus Source: Ensembl
    5. cGMP-mediated signaling Source: Ensembl
    6. diterpenoid metabolic process Source: Ensembl
    7. negative regulation of apoptotic process Source: Ensembl
    8. negative regulation of vascular permeability Source: Ensembl
    9. oocyte maturation Source: MGI
    10. positive regulation of oocyte development Source: MGI
    11. positive regulation of vascular permeability Source: Ensembl
    12. regulation of meiosis Source: MGI
    13. response to cAMP Source: Ensembl
    14. response to drug Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, cGMP, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-inhibited 3',5'-cyclic phosphodiesterase A (EC:3.1.4.17)
    Alternative name(s):
    Cyclic GMP-inhibited phosphodiesterase A
    Short name:
    CGI-PDE A
    Gene namesi
    Name:Pde3a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1860764. Pde3a.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11411141cGMP-inhibited 3',5'-cyclic phosphodiesterase APRO_0000198800Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei310 – 3101PhosphoserineBy similarity
    Modified residuei520 – 5201PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9Z0X4.
    PRIDEiQ9Z0X4.

    PTM databases

    PhosphoSiteiQ9Z0X4.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Z0X4.
    BgeeiQ9Z0X4.
    CleanExiMM_PDE3A.
    GenevestigatoriQ9Z0X4.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9Z0X4. 3 interactions.
    MINTiMINT-4049569.
    STRINGi10090.ENSMUSP00000038749.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z0X4.
    SMRiQ9Z0X4. Positions 677-1087.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei62 – 8221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei127 – 14721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei157 – 17721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei182 – 20221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei207 – 22721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei229 – 24921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni728 – 1086359CatalyticBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG145074.
    GeneTreeiENSGT00750000117528.
    HOGENOMiHOG000060144.
    HOVERGENiHBG053541.
    InParanoidiQ9Z0X4.
    KOiK13296.
    OMAiPETMMFL.
    OrthoDBiEOG7KDF95.
    PhylomeDBiQ9Z0X4.
    TreeFamiTF329631.

    Family and domain databases

    Gene3Di1.10.1300.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z0X4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVRGEAAQD LAKPGLGGAS PARVARGNHR HRGESSPSPR GSGCCWRALA     50
    LQPLRRSPQL SSALCAGSLS VLLALLVRLV GGEVGGELEK SQEAAAEEEE 100
    EEGARGGVFP GPRGGAPGGG AQLSPWLQPA ALLFSLLCAF FWMGLCLLRA 150
    GVRLPLAVAL LAACCAGEAL VQLSLGVGDG RLLSLPAAGV LLSCLGGATW 200
    LVLRLRLGVL MVAWTSVLRT VALVSLERFK VAWRPYLAYL AAVLGLLLAR 250
    YAEQILPQCS GPAPPRERFG SQLSARTKEE IPGWKRRRRS SSVVAGEMSG 300
    CSGKSHRRTS LPCIPREQLM GHSEWDHKRG PRGSQSGTSI TVDIAVMGEA 350
    HGLITDLLAD PSLPPNVCTS LRAVSNLLST QLTFQAIHKP RVNPTVTFSE 400
    NYTCSDSEEG LEKDKQAISK RLRRSLPPGL LRRVSSTWTT TTSATGLPTL 450
    EPAPVRRDRS ASIKPHEAPS PSAVNPDSWN APGLTTLTKS RSFTSSYAVS 500
    AANHVKAKKQ NRPGGLAKIS PVPSPSSSPP QGSPASSPVS NSASQQFPES 550
    PEVTIKRGPG SHRALTYTQS APDLSPQIPP PSVICSSCGR PYSQGNPADG 600
    PSERSGPAML KPNRTDDTSQ VTSDYETNNN SDSSDILQNE EEAECQREPQ 650
    RKASACGTYT SQTMIFLDKP ILAPEPLVMD NLDSIMDQLN TWNFPIFDLM 700
    ENIGRKCGRI LSQVSYRLFE DMGLFEAFKI PVREFMNYFH ALEIGYRDIP 750
    YHNRIHATDV LHAVWYLTTQ PIPGLPSVIG DHGSASDSDS DSGFTHGHMG 800
    YVFSKMYHVP DDKYGCLSGN IPALELMALY VAAAMHDYDH PGRTNAFLVA 850
    TSAPQAVLYN DRSVLENHHA AAAWNLFMSR PEYNFLVNLD HVEFKHFRFL 900
    VIEAILATDL KKHFDFVAKF NAKVNDDVGI DWTNENDRLL VCQMCIKLAD 950
    INGPAKCKEL HLRWTEGIAS EFYEQGDEEA SLGLPISPFM DRSAPQLANL 1000
    QESFISHIVG PLCHSYDSAG LMPGKWVDDS DDSGDTDDPE EEEEEAETPH 1050
    EDEACESSIA PRKKSFKRRR IYCQITQHLL QNHMMWKKVI EEEQCLSGTE 1100
    NQSLDQVPLQ HPSEQIQAIK EEEEEKGKPR AEETLAPQPD L 1141
    Length:1,141
    Mass (Da):124,513
    Last modified:May 1, 1999 - v1
    Checksum:i3D9ACCFF928219D8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF099187 mRNA. Translation: AAD16300.1.
    CCDSiCCDS20676.1.
    RefSeqiNP_061249.1. NM_018779.1.
    UniGeneiMm.103728.
    Mm.485878.

    Genome annotation databases

    EnsembliENSMUST00000043259; ENSMUSP00000038749; ENSMUSG00000041741.
    GeneIDi54611.
    KEGGimmu:54611.
    UCSCiuc009eon.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF099187 mRNA. Translation: AAD16300.1 .
    CCDSi CCDS20676.1.
    RefSeqi NP_061249.1. NM_018779.1.
    UniGenei Mm.103728.
    Mm.485878.

    3D structure databases

    ProteinModelPortali Q9Z0X4.
    SMRi Q9Z0X4. Positions 677-1087.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9Z0X4. 3 interactions.
    MINTi MINT-4049569.
    STRINGi 10090.ENSMUSP00000038749.

    PTM databases

    PhosphoSitei Q9Z0X4.

    Proteomic databases

    PaxDbi Q9Z0X4.
    PRIDEi Q9Z0X4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043259 ; ENSMUSP00000038749 ; ENSMUSG00000041741 .
    GeneIDi 54611.
    KEGGi mmu:54611.
    UCSCi uc009eon.1. mouse.

    Organism-specific databases

    CTDi 5139.
    MGIi MGI:1860764. Pde3a.

    Phylogenomic databases

    eggNOGi NOG145074.
    GeneTreei ENSGT00750000117528.
    HOGENOMi HOG000060144.
    HOVERGENi HBG053541.
    InParanoidi Q9Z0X4.
    KOi K13296.
    OMAi PETMMFL.
    OrthoDBi EOG7KDF95.
    PhylomeDBi Q9Z0X4.
    TreeFami TF329631.

    Miscellaneous databases

    NextBioi 311416.
    PROi Q9Z0X4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z0X4.
    Bgeei Q9Z0X4.
    CleanExi MM_PDE3A.
    Genevestigatori Q9Z0X4.

    Family and domain databases

    Gene3Di 1.10.1300.10. 2 hits.
    InterProi IPR003607. HD/PDEase_dom.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the cyclic guanosine monophosphate-inhibited phosphodiesterase PDE3A expressed in mouse oocyte."
      Shitsukawa K., Andersen C.B., Richard F.J., Horner A.K., Wiersma A., van Duin M., Conti M.
      Biol. Reprod. 65:188-196(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: C57BL/6J.
      Tissue: Ovary.

    Entry informationi

    Entry nameiPDE3A_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z0X4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3