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Protein

Chloride intracellular channel protein 4

Gene

Clic4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. May play a role in angiogenesis (By similarity).By similarity2 Publications

GO - Molecular functioni

  • chloride channel activity Source: RGD
  • protein complex binding Source: RGD
  • voltage-gated ion channel activity Source: UniProtKB-KW

GO - Biological processi

  • angiogenesis Source: GO_Central
  • apoptotic process Source: InterPro
  • chloride transport Source: UniProtKB
  • negative regulation of cell migration Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride intracellular channel protein 4
Alternative name(s):
Intracellular chloride ion channel protein p64H1
Gene namesi
Name:Clic4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61857. Clic4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei37 – 57Helical; Note=After insertion into the membraneSequence analysisAdd BLAST21

GO - Cellular componenti

  • chloride channel complex Source: UniProtKB-KW
  • cytoplasm Source: UniProtKB
  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: UniProtKB
  • integral component of membrane Source: RGD
  • mitochondrion Source: GO_Central
  • nucleus Source: GO_Central
  • plasma membrane Source: UniProtKB-SubCell
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001442122 – 253Chloride intracellular channel protein 4Add BLAST252

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei130N6-acetyllysineBy similarity1
Modified residuei236PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9Z0W7.
PRIDEiQ9Z0W7.

2D gel databases

World-2DPAGE0004:Q9Z0W7.

PTM databases

iPTMnetiQ9Z0W7.
PhosphoSitePlusiQ9Z0W7.

Expressioni

Tissue specificityi

Detected in brain, in cell bodies and dendrites of Purkinje cells in cerebellar neurons (at protein level). Marked expression was found in hippocampus and cerebellum, and in many other tissues.1 Publication

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with HRH3.By similarity1 Publication

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

BioGridi249813. 6 interactors.
MINTiMINT-214778.
STRINGi10116.ENSRNOP00000024464.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0W7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 244GST C-terminalAdd BLAST141

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 101Required for insertion into the membraneBy similarityAdd BLAST100

Domaini

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion (By similarity).By similarity

Sequence similaritiesi

Belongs to the chloride channel CLIC family.Curated
Contains 1 GST C-terminal domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1422. Eukaryota.
ENOG410ZRK6. LUCA.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiQ9Z0W7.
KOiK05024.
PhylomeDBiQ9Z0W7.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030257. CLIC-4.
IPR010987. Glutathione-S-Trfase_C-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF185. PTHR11260:SF185. 2 hits.
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0W7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSMPLNGL KEEDKEPLIE LFVKAGSDGE SIGNCPFSQR LFMILWLKGV
60 70 80 90 100
VFSVTTVDLK RKPAHLQNLA PGTHPPFITF NSEVKTDVNK IEEFLEEVLC
110 120 130 140 150
PPKYLKLSPK HPESNTAGMD IFAKFSAYIK NSRPEANEAL ERGLLKTLQK
160 170 180 190 200
LDEYLNSPLP GEIDENSMED IKSSTRRFLD GDEMTLADCN LLPKLHIVKV
210 220 230 240 250
VAKKYRNFDI PKGMTGIWRY LTNAYSRDEF TNTCPSDKEV EIAYSDVAKR

LTK
Length:253
Mass (Da):28,633
Last modified:January 23, 2007 - v3
Checksum:iDC2A66C3258AAFCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104119 mRNA. Translation: AAD16875.1.
PIRiS70484.
RefSeqiNP_114006.1. NM_031818.1.
UniGeneiRn.104756.

Genome annotation databases

GeneIDi83718.
KEGGirno:83718.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF104119 mRNA. Translation: AAD16875.1.
PIRiS70484.
RefSeqiNP_114006.1. NM_031818.1.
UniGeneiRn.104756.

3D structure databases

ProteinModelPortaliQ9Z0W7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249813. 6 interactors.
MINTiMINT-214778.
STRINGi10116.ENSRNOP00000024464.

PTM databases

iPTMnetiQ9Z0W7.
PhosphoSitePlusiQ9Z0W7.

2D gel databases

World-2DPAGE0004:Q9Z0W7.

Proteomic databases

PaxDbiQ9Z0W7.
PRIDEiQ9Z0W7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83718.
KEGGirno:83718.

Organism-specific databases

CTDi25932.
RGDi61857. Clic4.

Phylogenomic databases

eggNOGiKOG1422. Eukaryota.
ENOG410ZRK6. LUCA.
HOGENOMiHOG000231548.
HOVERGENiHBG050994.
InParanoidiQ9Z0W7.
KOiK05024.
PhylomeDBiQ9Z0W7.

Miscellaneous databases

PROiQ9Z0W7.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR002946. CLIC.
IPR030257. CLIC-4.
IPR010987. Glutathione-S-Trfase_C-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11260:SF185. PTHR11260:SF185. 2 hits.
PRINTSiPR01263. INTCLCHANNEL.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00862. O-ClC. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLIC4_RAT
AccessioniPrimary (citable) accession number: Q9Z0W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.