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Protein

Peroxiredoxin-4

Gene

Prdx4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.By similarity

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei126Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BRENDAi1.11.1.15. 5301.
ReactomeiR-RNO-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei4533. Rno2CysPrx04.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-4 (EC:1.11.1.15)
Alternative name(s):
Antioxidant enzyme AOE372
Peroxiredoxin IV
Short name:
Prx-IV
Thioredoxin peroxidase AO372
Thioredoxin-dependent peroxide reductase A0372
Gene namesi
Name:Prdx4
Synonyms:Rno2CysPrx04
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi620043. Prdx4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 40By similarityAdd BLAST40
ChainiPRO_000039087741 – 273Peroxiredoxin-4Add BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101N6-succinyllysineBy similarity1
Disulfide bondi126Interchain (with C-247); in linked formBy similarity
Disulfide bondi247Interchain (with C-126); in linked formBy similarity

Post-translational modificationi

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9Z0V5.
PRIDEiQ9Z0V5.

PTM databases

iPTMnetiQ9Z0V5.
PhosphoSitePlusiQ9Z0V5.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003763.
GenevisibleiQ9Z0V5. RN.

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9Z0V5. 2 interactors.
STRINGi10116.ENSRNOP00000005014.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0V5.
SMRiQ9Z0V5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 239ThioredoxinPROSITE-ProRule annotationAdd BLAST159

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

eggNOGiKOG0852. Eukaryota.
COG0450. LUCA.
GeneTreeiENSGT00390000004653.
HOGENOMiHOG000022343.
HOVERGENiHBG000286.
InParanoidiQ9Z0V5.
KOiK03386.
OMAiGWKPGQD.
OrthoDBiEOG091G0IE5.
PhylomeDBiQ9Z0V5.
TreeFamiTF105181.

Family and domain databases

InterProiView protein in InterPro
IPR000866. AhpC/TSA.
IPR024706. Peroxiredoxin_AhpC-typ.
IPR019479. Peroxiredoxin_C.
IPR036249. Thioredoxin-like_sf.
IPR013766. Thioredoxin_domain.
PfamiView protein in Pfam
PF10417. 1-cysPrx_C. 1 hit.
PF00578. AhpC-TSA. 1 hit.
PIRSFiPIRSF000239. AHPC. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS51352. THIOREDOXIN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0V5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METWSKLLDG TTPSRRWRKL VLLLPPLLLF LLQTEALQGL ESDDRFRTRE
60 70 80 90 100
NECHFYAGGQ VYPGEVSRVS VADHSLHLSK AKISKPAPYW EGTAVINGEF
110 120 130 140 150
KELKLTDYRG KYLVFFFYPL DFTFVCPTEI IAFGDRIEEF KSINTEVVAC
160 170 180 190 200
SVDSQFTHLA WINTPRRQGG LGPIRIPLLS DLNHQISKDY GVYLEDSGHT
210 220 230 240 250
LRGLFIIDDK GVLRQITLND LPVGRSVDET LRLVQAFQYT DKHGEVCPAG
260 270
WKPGSETIIP DPAGKLKYFD KLN
Length:273
Mass (Da):31,007
Last modified:May 1, 1999 - v1
Checksum:i09E614794F1DC6C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106945 mRNA. Translation: AAD17993.1.
BC059122 mRNA. Translation: AAH59122.1.
RefSeqiNP_445964.1. NM_053512.2.
UniGeneiRn.17958.

Genome annotation databases

EnsembliENSRNOT00000005014; ENSRNOP00000005014; ENSRNOG00000003763.
GeneIDi85274.
KEGGirno:85274.

Similar proteinsi

Entry informationi

Entry nameiPRDX4_RAT
AccessioniPrimary (citable) accession number: Q9Z0V5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: May 1, 1999
Last modified: November 22, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families