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Protein

Peroxiredoxin-4

Gene

Prdx4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.By similarity

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.By similarity

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei126Cysteine sulfenic acid (-SOH) intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAntioxidant, Oxidoreductase, Peroxidase

Enzyme and pathway databases

BRENDAi1.11.1.15 5301
ReactomeiR-RNO-6798695 Neutrophil degranulation

Protein family/group databases

PeroxiBasei4533 Rno2CysPrx04

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxiredoxin-4 (EC:1.11.1.15)
Alternative name(s):
Antioxidant enzyme AOE372
Peroxiredoxin IV
Short name:
Prx-IV
Thioredoxin peroxidase AO372
Thioredoxin-dependent peroxide reductase A0372
Gene namesi
Name:Prdx4
Synonyms:Rno2CysPrx04
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi620043 Prdx4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 40By similarityAdd BLAST40
ChainiPRO_000039087741 – 273Peroxiredoxin-4Add BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi126Interchain (with C-247); in linked formBy similarity
Disulfide bondi247Interchain (with C-126); in linked formBy similarity

Post-translational modificationi

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9Z0V5
PRIDEiQ9Z0V5

PTM databases

CarbonylDBiQ9Z0V5
iPTMnetiQ9Z0V5
PhosphoSitePlusiQ9Z0V5

Expressioni

Gene expression databases

BgeeiENSRNOG00000003763
GenevisibleiQ9Z0V5 RN

Interactioni

Subunit structurei

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9Z0V5, 2 interactors
STRINGi10116.ENSRNOP00000005014

Structurei

3D structure databases

ProteinModelPortaliQ9Z0V5
SMRiQ9Z0V5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 239ThioredoxinPROSITE-ProRule annotationAdd BLAST159

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

eggNOGiKOG0852 Eukaryota
COG0450 LUCA
GeneTreeiENSGT00390000004653
HOGENOMiHOG000022343
HOVERGENiHBG000286
InParanoidiQ9Z0V5
KOiK03386
OMAiGWKPGQD
OrthoDBiEOG091G0IE5
PhylomeDBiQ9Z0V5
TreeFamiTF105181

Family and domain databases

InterProiView protein in InterPro
IPR000866 AhpC/TSA
IPR024706 Peroxiredoxin_AhpC-typ
IPR019479 Peroxiredoxin_C
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF10417 1-cysPrx_C, 1 hit
PF00578 AhpC-TSA, 1 hit
PIRSFiPIRSF000239 AHPC, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0V5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METWSKLLDG TTPSRRWRKL VLLLPPLLLF LLQTEALQGL ESDDRFRTRE
60 70 80 90 100
NECHFYAGGQ VYPGEVSRVS VADHSLHLSK AKISKPAPYW EGTAVINGEF
110 120 130 140 150
KELKLTDYRG KYLVFFFYPL DFTFVCPTEI IAFGDRIEEF KSINTEVVAC
160 170 180 190 200
SVDSQFTHLA WINTPRRQGG LGPIRIPLLS DLNHQISKDY GVYLEDSGHT
210 220 230 240 250
LRGLFIIDDK GVLRQITLND LPVGRSVDET LRLVQAFQYT DKHGEVCPAG
260 270
WKPGSETIIP DPAGKLKYFD KLN
Length:273
Mass (Da):31,007
Last modified:May 1, 1999 - v1
Checksum:i09E614794F1DC6C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106945 mRNA Translation: AAD17993.1
BC059122 mRNA Translation: AAH59122.1
RefSeqiNP_445964.1, NM_053512.2
UniGeneiRn.17958

Genome annotation databases

EnsembliENSRNOT00000005014; ENSRNOP00000005014; ENSRNOG00000003763
GeneIDi85274
KEGGirno:85274

Similar proteinsi

Entry informationi

Entry nameiPRDX4_RAT
AccessioniPrimary (citable) accession number: Q9Z0V5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health