Q9Z0V1 (KCND3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily D member 3

Alternative name(s):
Voltage-gated potassium channel subunit Kv4.3

Gene names

Name:

Kcnd3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.
Subunit structure	Homotetramer or heterotetramer with KCND1 and/or KCND2. Associates with the regulatory subunits KCNIP1, KCNIP2, KCNIP3 and KCNIP4. Interacts with DLG1, KCNE1, KCNE2, SCN1B and KCNAB1 By similarity. Ref.3 Ref.4
Subcellular location	Membrane; Multi-pass membrane protein. Cell membrane › sarcolemma By similarity. Cell projection › dendrite By similarity.
Domain	The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Post-translational modification	Regulated through phosphorylation at Ser-569 by CaMK2D.
Sequence similarities	Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.3/KCND3 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Cell membrane Cell projection Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Metal-binding Potassium Zinc
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein homooligomerization Inferred from electronic annotation. Source: InterPro
Cellular_component	dendrite Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from direct assay PubMed 18603586. Source: MGI neuronal cell body Inferred from electronic annotation. Source: Compara sarcolemma Inferred from electronic annotation. Source: UniProtKB-SubCell voltage-gated potassium channel complex Inferred from electronic annotation. Source: InterPro
Molecular_function	A-type (transient outward) potassium channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9Z0V1-1)

Also known as: Kv4.3L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9Z0V1-2)

Also known as: Kv4.3M;

The sequence of this isoform differs from the canonical sequence as follows:
     488-506: Missing.

Isoform 3 (identifier: Q9Z0V1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     488-531: GLSYLVDDPL...ESSMQNYPST → VSSSLLPPPA...SKTIVSLPLG
     532-655: Missing.

Note: May be due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 655	655	Potassium voltage-gated channel subfamily D member 3		PRO_0000054069
Regions
	Topological domain	1 – 181	181	Cytoplasmic Potential
	Transmembrane	182 – 202	21	Helical; Name=Segment S1; Potential
	Transmembrane	222 – 242	21	Helical; Name=Segment S2; Potential
	Topological domain	243 – 256	14	Cytoplasmic Potential
	Transmembrane	257 – 277	21	Helical; Name=Segment S3; Potential
	Transmembrane	287 – 307	21	Helical; Voltage-sensor; Name=Segment S4; Potential
	Topological domain	308 – 320	13	Cytoplasmic Potential
	Transmembrane	321 – 341	21	Helical; Name=Segment S5; Potential
	Intramembrane	360 – 380	21	Pore-forming; Name=Segment H5; Potential
	Transmembrane	382 – 402	21	Helical; Name=Segment S6; Potential
	Topological domain	403 – 655	253	Cytoplasmic Potential
	Region	2 – 20	19	Interaction with KCNIP2 By similarity
	Region	472 – 487	16	Mediates dendritic targeting By similarity
	Motif	367 – 372	6	Selectivity filter By similarity
Sites
	Metal binding	104	1	Zinc By similarity
	Metal binding	131	1	Zinc By similarity
	Metal binding	132	1	Zinc By similarity
Amino acid modifications
	Modified residue	569	1	Phosphoserine; by CaMK2D By similarity
Natural variations
	Alternative sequence	488 – 531	44	GLSYL…NYPST → VSSSLLPPPASSLTSQGCTH VIIPRRESSSVPFQSKTIVS LPLG in isoform 3.		VSP_008828
	Alternative sequence	488 – 506	19	Missing in isoform 2.		VSP_008827
	Alternative sequence	532 – 655	124	Missing in isoform 3.		VSP_008829

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Kv4.3L) [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 17FCE5AEC2868B33	FASTA	655	73,462
	10 20 30 40 50 60 MAAGVAAWLP FARAAAIGWM PVANCPMPLA PADKNKRQDE LIVLNVSGRR FQTWRTTLER 70 80 90 100 110 120 YPDTLLGSTE KEFFFNEDTK EYFFDRDPEV FRCVLNFYRT GKLHYPRYEC ISAYDDELAF 130 140 150 160 170 180 YGILPEIIGD CCYEEYKDRK RENAERLMDD NDSENNQESM PSLSFRQTMW RAFENPHTST 190 200 210 220 230 240 LALVFYYVTG FFIAVSVITN VVETVPCGTV PGSKELPCGE RYSVAFFCLD TACVMIFTVE 250 260 270 280 290 300 YLLRLFAAPS RYRFIRSVMS IIDVVAIMPY YIGLVMTNNE DVSGAFVTLR VFRVFRIFKF 310 320 330 340 350 360 SRHSQGLRIL GYTLKSCASE LGFLLFSLTM AIIIFATVMF YAEKGSSASK FTSIPASFWY 370 380 390 400 410 420 TIVTMTTLGY GDMVPKTIAG KIFGSICSLS GVLVIALPVP VIVSNFSRIY HQNQRADKRR 430 440 450 460 470 480 AQKKARLARI RVAKTGSSNA YLHSKRNGLL NEALELTGTP EEEQMGKTTS LIESQHHHLL 490 500 510 520 530 540 HCLEKTTGLS YLVDDPLLSV RTSTIKNHEF IDEQMFEQNC MESSMQNYPS TRSPSLSSHS 550 560 570 580 590 600 GLTTTCCSRR SKKTTHLPNS NLPATRLRSM QELSTLHIQG SEQPSLTTSR SSLNLKADDG 610 620 630 640 650 LRPNCKTSQI TTAIISIPTP PALTPEGESR PPPASPGPNT NIPSITSNVV KVSAL
	Isoform 2 (Kv4.3M) [UniParc]. Checksum: ABC972B74CA982AA	FASTA	636	71,403
	10 20 30 40 50 60 MAAGVAAWLP FARAAAIGWM PVANCPMPLA PADKNKRQDE LIVLNVSGRR FQTWRTTLER 70 80 90 100 110 120 YPDTLLGSTE KEFFFNEDTK EYFFDRDPEV FRCVLNFYRT GKLHYPRYEC ISAYDDELAF 130 140 150 160 170 180 YGILPEIIGD CCYEEYKDRK RENAERLMDD NDSENNQESM PSLSFRQTMW RAFENPHTST 190 200 210 220 230 240 LALVFYYVTG FFIAVSVITN VVETVPCGTV PGSKELPCGE RYSVAFFCLD TACVMIFTVE 250 260 270 280 290 300 YLLRLFAAPS RYRFIRSVMS IIDVVAIMPY YIGLVMTNNE DVSGAFVTLR VFRVFRIFKF 310 320 330 340 350 360 SRHSQGLRIL GYTLKSCASE LGFLLFSLTM AIIIFATVMF YAEKGSSASK FTSIPASFWY 370 380 390 400 410 420 TIVTMTTLGY GDMVPKTIAG KIFGSICSLS GVLVIALPVP VIVSNFSRIY HQNQRADKRR 430 440 450 460 470 480 AQKKARLARI RVAKTGSSNA YLHSKRNGLL NEALELTGTP EEEQMGKTTS LIESQHHHLL 490 500 510 520 530 540 HCLEKTTNHE FIDEQMFEQN CMESSMQNYP STRSPSLSSH SGLTTTCCSR RSKKTTHLPN 550 560 570 580 590 600 SNLPATRLRS MQELSTLHIQ GSEQPSLTTS RSSLNLKADD GLRPNCKTSQ ITTAIISIPT 610 620 630 PPALTPEGES RPPPASPGPN TNIPSITSNV VKVSAL
	Isoform 3 [UniParc]. Checksum: 5580144E7899BE5F	FASTA	531	59,862
	10 20 30 40 50 60 MAAGVAAWLP FARAAAIGWM PVANCPMPLA PADKNKRQDE LIVLNVSGRR FQTWRTTLER 70 80 90 100 110 120 YPDTLLGSTE KEFFFNEDTK EYFFDRDPEV FRCVLNFYRT GKLHYPRYEC ISAYDDELAF 130 140 150 160 170 180 YGILPEIIGD CCYEEYKDRK RENAERLMDD NDSENNQESM PSLSFRQTMW RAFENPHTST 190 200 210 220 230 240 LALVFYYVTG FFIAVSVITN VVETVPCGTV PGSKELPCGE RYSVAFFCLD TACVMIFTVE 250 260 270 280 290 300 YLLRLFAAPS RYRFIRSVMS IIDVVAIMPY YIGLVMTNNE DVSGAFVTLR VFRVFRIFKF 310 320 330 340 350 360 SRHSQGLRIL GYTLKSCASE LGFLLFSLTM AIIIFATVMF YAEKGSSASK FTSIPASFWY 370 380 390 400 410 420 TIVTMTTLGY GDMVPKTIAG KIFGSICSLS GVLVIALPVP VIVSNFSRIY HQNQRADKRR 430 440 450 460 470 480 AQKKARLARI RVAKTGSSNA YLHSKRNGLL NEALELTGTP EEEQMGKTTS LIESQHHHLL 490 500 510 520 530 HCLEKTTVSS SLLPPPASSL TSQGCTHVII PRRESSSVPF QSKTIVSLPL G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional characterization of mouse heart K+ channel alpha subunits, Kv1.5, Kv4.2 and Kv4.3." Tanaka H., Janzen K., Winkfein R.J., Fiset C., Clark R.B., Giles W.R. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: Swiss Webster. Tissue: Heart ventricle.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6J. Tissue: Diencephalon.
[3]	"Tuning pacemaker frequency of individual dopaminergic neurons by Kv4.3L and KChip3.1 transcription." Liss B., Franz O., Sewing S., Bruns R., Neuhoff H., Roeper J. EMBO J. 20:5715-5724(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KCNIP3.
[4]	"Role of heteromultimers in the generation of myocardial transient outward K+ currents." Guo W., Li H., Aimond F., Johns D.C., Rhodes K.J., Trimmer J.S., Nerbonne J.M. Circ. Res. 90:586-593(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KCND2 AND KCNIP2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF107781 mRNA. Translation: AAD16973.1. AF107782 mRNA. Translation: AAD16974.1. AK033962 mRNA. Translation: BAC28529.1.
IPI	IPI00129489. IPI00282762. IPI00387305.
RefSeq	NP_001034436.1. NM_001039347.1. NP_064315.1. NM_019931.1.
UniGene	Mm.44530.
3D structure databases
ProteinModelPortal	Q9Z0V1.
SMR	Q9Z0V1. Positions 6-412.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000113436.
PTM databases
PhosphoSite	Q9Z0V1.
Proteomic databases
PaxDb	Q9Z0V1.
PRIDE	Q9Z0V1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000079169; ENSMUSP00000078169; ENSMUSG00000040896. ENSMUST00000098761; ENSMUSP00000096357; ENSMUSG00000040896. ENSMUST00000118360; ENSMUSP00000113436; ENSMUSG00000040896.
GeneID	56543.
KEGG	mmu:56543.
UCSC	uc008qux.1. mouse.
Organism-specific databases
CTD	3752.
MGI	MGI:1928743. Kcnd3.
Phylogenomic databases
eggNOG	COG1226.
GeneTree	ENSGT00630000089518.
HOGENOM	HOG000231013.
HOVERGEN	HBG106687.
InParanoid	Q9Z0V1.
KO	K04893.
OMA	MPLAPTE.
OrthoDB	EOG4HMJ8X.
Gene expression databases
ArrayExpress	Q9Z0V1.
Bgee	Q9Z0V1.
Genevestigator	Q9Z0V1.
GermOnline	ENSMUSG00000040896. Mus musculus.
Family and domain databases
Gene3D	3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR005821. Ion_trans_dom. IPR003091. K_chnl. IPR003968. K_chnl_volt-dep_Kv. IPR003975. K_chnl_volt-dep_Kv4. IPR004056. K_chnl_volt-dep_Kv4.3. IPR024587. K_chnl_volt-dep_Kv4_C. IPR021645. Shal-type. IPR003131. T1-type_BTB. [Graphical view]
PANTHER	PTHR11537. PTHR11537. 1 hit.
Pfam	PF11879. DUF3399. 1 hit. PF00520. Ion_trans. 1 hit. PF02214. K_tetra. 1 hit. PF11601. Shal-type. 1 hit. [Graphical view]
PRINTS	PR00169. KCHANNEL. PR01518. KV43CHANNEL. PR01491. KVCHANNEL. PR01497. SHALCHANNEL.
SMART	SM00225. BTB. 1 hit. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	312914.
SOURCE	Search...

Entry information

Entry name

KCND3_MOUSE

Accession

Primary (citable) accession number: Q9Z0V1
Secondary accession number(s): Q8CC44, Q9Z0V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program