Q9Z0U4 (GABR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-aminobutyric acid type B receptor subunit 1
Short name=GABA-B receptor 1
Short name=GABA-B-R1
Short name=GABA-BR1
Short name=GABABR1
Short name=Gb1

Gene names

Name:

Gabbr1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	991 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for GABA. The activity of this receptor is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA-B-R inhibit neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA-B-R decrease neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.
Cofactor	Calcium. Required for high affinity binding to GABA.
Subunit structure	Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Interacts with the leucine zipper of the C-terminal bZIP domain of ATF4 via its C-terminal region. Interacts with JAKMIP1. Ref.4 Ref.6 Ref.7 Ref.9
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note: Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes. Ref.7
Tissue specificity	Isoform 1A, isoform 1B and isoform 1C are expressed in testis, stomach, spinal cord and brain including cerebral cortical layers, pyramidal cell layers of the hippocampus, granular cell layers of the dentate gyrus, basal ganglia, cerebellum (predominantly in Purkinje cells followed by granular layer). Isoform 1B is also expressed in kidney and liver. Isoform 1D is expressed in forebrain, cerebellum, eye, kidney, and urinary bladder. Ref.5
Domain	Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
Sequence similarities	Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. Contains 2 Sushi (CCP/SCR) domains.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of adenylate cyclase activity Inferred from direct assay Ref.1. Source: MGI negative regulation of cell proliferation Inferred from mutant phenotype PubMed 15147298. Source: RGD negative regulation of dopamine secretion Inferred from direct assay PubMed 12872287. Source: RGD negative regulation of epinephrine secretion Inferred from mutant phenotype PubMed 14568556. Source: RGD negative regulation of gamma-aminobutyric acid secretion Inferred from mutant phenotype PubMed 14654095. Source: RGD negative regulation of synaptic transmission Inferred from direct assay PubMed 12604336. Source: RGD osteoblast differentiation Inferred from mutant phenotype PubMed 12054677. Source: RGD positive regulation of glutamate secretion Inferred from mutant phenotype PubMed 18765663. Source: RGD positive regulation of growth hormone secretion Inferred from mutant phenotype PubMed 12218349. Source: RGD response to ethanol Inferred from expression pattern PubMed 16246313. Source: RGD response to nicotine Inferred from expression pattern PubMed 12435490. Source: RGD
Cellular_component	axolemma Inferred from direct assay PubMed 17924569. Source: RGD cell junction Inferred from electronic annotation. Source: UniProtKB-KW dendritic shaft Inferred from direct assay PubMed 17241134. Source: RGD dendritic spine Inferred from direct assay PubMed 16983643. Source: RGD endoplasmic reticulum membrane Inferred from direct assay PubMed 17924569. Source: RGD extracellular space Inferred from direct assay PubMed 18765663. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane raft Inferred from direct assay PubMed 19054408. Source: RGD mitochondrial membrane Inferred from direct assay PubMed 17924569. Source: RGD neuronal cell body Inferred from direct assay PubMed 17241134. Source: RGD postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synaptic vesicle Inferred from direct assay PubMed 17924569. Source: RGD
Molecular_function	G-protein coupled GABA receptor activity Inferred from direct assay Ref.3. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1E (identifier: Q9Z0U4-1)

Also known as: 1C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1A (identifier: Q9Z0U4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     771-801: Missing.

Isoform 1B (identifier: Q9Z0U4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: MLLLLLVPLF...PHCQVNRTPH → MGPGGPCTPV...PHPRVPPHPS
     771-801: Missing.

Isoform 1C (identifier: Q9Z0U4-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: MLLLLLVPLF...PHCQVNRTPH → MGPGGPCTPV...PHPRVPPHPS

Isoform 1D (identifier: Q9Z0U4-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: MLLLLLVPLF...PHCQVNRTPH → MGPGGPCTPV...PHPRVPPHPS
     771-801: Missing.
     935-991: KEERVSELRH...DGSRVHLLYK → VCGDKQPGPPVSEGGLPVVGPSIEV

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

16

Potential

Chain

17 – 991

975

Gamma-aminobutyric acid type B receptor subunit 1

PRO_0000012951

Regions

Topological domain

17 – 590

574

Extracellular Potential

Transmembrane

591 – 611

21

Helical; Name=1; Potential

Topological domain

612 – 630

19

Cytoplasmic Potential

Transmembrane

631 – 651

21

Helical; Name=2; Potential

Topological domain

652 – 666

15

Extracellular Potential

Transmembrane

667 – 687

21

Helical; Name=3; Potential

Topological domain

688 – 709

22

Cytoplasmic Potential

Transmembrane

710 – 730

21

Helical; Name=4; Potential

Topological domain

731 – 797

67

Extracellular Potential

Transmembrane

798 – 818

21

Helical; Name=5; Potential

Topological domain

819 – 834

16

Cytoplasmic Potential

Transmembrane

835 – 855

21

Helical; Name=6; Potential

Topological domain

856 – 863

8

Extracellular Potential

Transmembrane

864 – 884

21

Helical; Name=7; Potential

Topological domain

885 – 991

107

Cytoplasmic Potential

Domain

29 – 95

67

Sushi 1

Domain

97 – 158

62

Sushi 2

Region

918 – 946

29

Interaction with ATF4

Coiled coil

901 – 955

55

Potential

Amino acid modifications

Modified residue

960

1

Phosphothreonine By similarity

Glycosylation

23

1

N-linked (GlcNAc...) Potential

Glycosylation

83

1

N-linked (GlcNAc...) Potential

Glycosylation

408

1

N-linked (GlcNAc...) Potential

Glycosylation

439

1

N-linked (GlcNAc...) Potential

Glycosylation

481

1

N-linked (GlcNAc...) Potential

Glycosylation

501

1

N-linked (GlcNAc...) Potential

Glycosylation

513

1

N-linked (GlcNAc...) Potential

Disulfide bond

99 ↔ 144

Ref.10

Disulfide bond

130 ↔ 156

Ref.10

Natural variations

Alternative sequence

1 – 163

163

MLLLL…NRTPH → MGPGGPCTPVGWPLPLLLVM AAGVAPVWASHSPHLPRPHP RVPPHPS in isoform 1B, isoform 1C and isoform 1D.

VSP_002044

Alternative sequence

771 – 801

31

Missing in isoform 1A, isoform 1B and isoform 1D.

VSP_002045

Alternative sequence

935 – 991

57

KEERV…HLLYK → VCGDKQPGPPVSEGGLPVVG PSIEV in isoform 1D.

VSP_002046

Experimental info

Mutagenesis

247

1

S → A: No change in the affinity for GABA. Ref.8

Mutagenesis

268

1

S → A: No change in the affinity for GABA. Ref.8

Mutagenesis

269

1

S → A: Decrease in the affinity for GABA. Ref.8

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

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.

.

.

.

.

991

Helix Strand Turn

Details...

Beta strand

96 – 98

3

Helix

101 – 104

4

Beta strand

109 – 112

4

Turn

120 – 123

4

Beta strand

125 – 130

6

Beta strand

134 – 136

3

Beta strand

141 – 145

5

Beta strand

148 – 151

4

Beta strand

156 – 158

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1E (1C) [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 012CD293D4B444A2	FASTA	991	111,534
	10 20 30 40 50 60 MLLLLLVPLF LRPLGAGGAQ TPNATSEGCQ IIHPPWEGGI RYRGLTRDQV KAINFLPVDY 70 80 90 100 110 120 EIEYVCRGER EVVGPKVRKC LANGSWTDMD TPSRCVRICS KSYLTLENGK VFLTGGDLPA 130 140 150 160 170 180 LDGARVEFRC DPDFHLVGSS RSVCSQGQWS TPKPHCQVNR TPHSERRAVY IGALFPMSGG 190 200 210 220 230 240 WPGGQACQPA VEMALEDVNS RRDILPDYEL KLIHHDSKCD PGQATKYLYE LLYNDPIKII 250 260 270 280 290 300 LMPGCSSVST LVAEAARMWN LIVLSYGSSS PALSNRQRFP TFFRTHPSAT LHNPTRVKLF 310 320 330 340 350 360 EKWGWKKIAT IQQTTEVFTS TLDDLEERVK EAGIEITFRQ SFFSDPAVPV KNLKRQDARI 370 380 390 400 410 420 IVGLFYETEA RKVFCEVYKE RLFGKKYVWF LIGWYADNWF KTYDPSINCT VEEMTEAVEG 430 440 450 460 470 480 HITTEIVMLN PANTRSISNM TSQEFVEKLT KRLKRHPEET GGFQEAPLAY DAIWALALAL 490 500 510 520 530 540 NKTSGGGGRS GVRLEDFNYN NQTITDQIYR AMNSSSFEGV SGHVVFDASG SRMAWTLIEQ 550 560 570 580 590 600 LQGGSYKKIG YYDSTKDDLS WSKTDKWIGG SPPADQTLVI KTFRFLSQKL FISVSVLSSL 610 620 630 640 650 660 GIVLAVVCLS FNIYNSHVRY IQNSQPNLNN LTAVGCSLAL AAVFPLGLDG YHIGRSQFPF 670 680 690 700 710 720 VCQARLWLLG LGFSLGYGSM FTKIWWVHTV FTKKEEKKEW RKTLEPWKLY ATVGLLVGMD 730 740 750 760 770 780 VLTLAIWQIV DPLHRTIETF AKEEPKEDID VSILPQLEHC SSKKMNTWLG ELWSFAVSSD 790 800 810 820 830 840 VQRRATVGGD SPICVWPAPE SIFYGYKGLL LLLGIFLAYE TKSVSTEKIN DHRAVGMAIY 850 860 870 880 890 900 NVAVLCLITA PVTMILSSQQ DAAFAFASLA IVFSSYITLV VLFVPKMRRL ITRGEWQSET 910 920 930 940 950 960 QDTMKTGSST NNNEEEKSRL LEKENRELEK IIAEKEERVS ELRHQLQSRQ QLRSRRHPPT 970 980 990 PPDPSGGLPR GPSEPPDRLS CDGSRVHLLY K
	Isoform 1A [UniParc]. Checksum: 05B7FBBCBE9DBF94	FASTA	960	108,204
	10 20 30 40 50 60 MLLLLLVPLF LRPLGAGGAQ TPNATSEGCQ IIHPPWEGGI RYRGLTRDQV KAINFLPVDY 70 80 90 100 110 120 EIEYVCRGER EVVGPKVRKC LANGSWTDMD TPSRCVRICS KSYLTLENGK VFLTGGDLPA 130 140 150 160 170 180 LDGARVEFRC DPDFHLVGSS RSVCSQGQWS TPKPHCQVNR TPHSERRAVY IGALFPMSGG 190 200 210 220 230 240 WPGGQACQPA VEMALEDVNS RRDILPDYEL KLIHHDSKCD PGQATKYLYE LLYNDPIKII 250 260 270 280 290 300 LMPGCSSVST LVAEAARMWN LIVLSYGSSS PALSNRQRFP TFFRTHPSAT LHNPTRVKLF 310 320 330 340 350 360 EKWGWKKIAT IQQTTEVFTS TLDDLEERVK EAGIEITFRQ SFFSDPAVPV KNLKRQDARI 370 380 390 400 410 420 IVGLFYETEA RKVFCEVYKE RLFGKKYVWF LIGWYADNWF KTYDPSINCT VEEMTEAVEG 430 440 450 460 470 480 HITTEIVMLN PANTRSISNM TSQEFVEKLT KRLKRHPEET GGFQEAPLAY DAIWALALAL 490 500 510 520 530 540 NKTSGGGGRS GVRLEDFNYN NQTITDQIYR AMNSSSFEGV SGHVVFDASG SRMAWTLIEQ 550 560 570 580 590 600 LQGGSYKKIG YYDSTKDDLS WSKTDKWIGG SPPADQTLVI KTFRFLSQKL FISVSVLSSL 610 620 630 640 650 660 GIVLAVVCLS FNIYNSHVRY IQNSQPNLNN LTAVGCSLAL AAVFPLGLDG YHIGRSQFPF 670 680 690 700 710 720 VCQARLWLLG LGFSLGYGSM FTKIWWVHTV FTKKEEKKEW RKTLEPWKLY ATVGLLVGMD 730 740 750 760 770 780 VLTLAIWQIV DPLHRTIETF AKEEPKEDID VSILPQLEHC SSKKMNTWLG IFYGYKGLLL 790 800 810 820 830 840 LLGIFLAYET KSVSTEKIND HRAVGMAIYN VAVLCLITAP VTMILSSQQD AAFAFASLAI 850 860 870 880 890 900 VFSSYITLVV LFVPKMRRLI TRGEWQSETQ DTMKTGSSTN NNEEEKSRLL EKENRELEKI 910 920 930 940 950 960 IAEKEERVSE LRHQLQSRQQ LRSRRHPPTP PDPSGGLPRG PSEPPDRLSC DGSRVHLLYK
	Isoform 1B [UniParc]. Checksum: B3417F53BE4877E9	FASTA	844	95,038
	10 20 30 40 50 60 MGPGGPCTPV GWPLPLLLVM AAGVAPVWAS HSPHLPRPHP RVPPHPSSER RAVYIGALFP 70 80 90 100 110 120 MSGGWPGGQA CQPAVEMALE DVNSRRDILP DYELKLIHHD SKCDPGQATK YLYELLYNDP 130 140 150 160 170 180 IKIILMPGCS SVSTLVAEAA RMWNLIVLSY GSSSPALSNR QRFPTFFRTH PSATLHNPTR 190 200 210 220 230 240 VKLFEKWGWK KIATIQQTTE VFTSTLDDLE ERVKEAGIEI TFRQSFFSDP AVPVKNLKRQ 250 260 270 280 290 300 DARIIVGLFY ETEARKVFCE VYKERLFGKK YVWFLIGWYA DNWFKTYDPS INCTVEEMTE 310 320 330 340 350 360 AVEGHITTEI VMLNPANTRS ISNMTSQEFV EKLTKRLKRH PEETGGFQEA PLAYDAIWAL 370 380 390 400 410 420 ALALNKTSGG GGRSGVRLED FNYNNQTITD QIYRAMNSSS FEGVSGHVVF DASGSRMAWT 430 440 450 460 470 480 LIEQLQGGSY KKIGYYDSTK DDLSWSKTDK WIGGSPPADQ TLVIKTFRFL SQKLFISVSV 490 500 510 520 530 540 LSSLGIVLAV VCLSFNIYNS HVRYIQNSQP NLNNLTAVGC SLALAAVFPL GLDGYHIGRS 550 560 570 580 590 600 QFPFVCQARL WLLGLGFSLG YGSMFTKIWW VHTVFTKKEE KKEWRKTLEP WKLYATVGLL 610 620 630 640 650 660 VGMDVLTLAI WQIVDPLHRT IETFAKEEPK EDIDVSILPQ LEHCSSKKMN TWLGIFYGYK 670 680 690 700 710 720 GLLLLLGIFL AYETKSVSTE KINDHRAVGM AIYNVAVLCL ITAPVTMILS SQQDAAFAFA 730 740 750 760 770 780 SLAIVFSSYI TLVVLFVPKM RRLITRGEWQ SETQDTMKTG SSTNNNEEEK SRLLEKENRE 790 800 810 820 830 840 LEKIIAEKEE RVSELRHQLQ SRQQLRSRRH PPTPPDPSGG LPRGPSEPPD RLSCDGSRVH LLYK
	Isoform 1C [UniParc]. Checksum: E7E9EDA9132D37F2	FASTA	875	98,368
	10 20 30 40 50 60 MGPGGPCTPV GWPLPLLLVM AAGVAPVWAS HSPHLPRPHP RVPPHPSSER RAVYIGALFP 70 80 90 100 110 120 MSGGWPGGQA CQPAVEMALE DVNSRRDILP DYELKLIHHD SKCDPGQATK YLYELLYNDP 130 140 150 160 170 180 IKIILMPGCS SVSTLVAEAA RMWNLIVLSY GSSSPALSNR QRFPTFFRTH PSATLHNPTR 190 200 210 220 230 240 VKLFEKWGWK KIATIQQTTE VFTSTLDDLE ERVKEAGIEI TFRQSFFSDP AVPVKNLKRQ 250 260 270 280 290 300 DARIIVGLFY ETEARKVFCE VYKERLFGKK YVWFLIGWYA DNWFKTYDPS INCTVEEMTE 310 320 330 340 350 360 AVEGHITTEI VMLNPANTRS ISNMTSQEFV EKLTKRLKRH PEETGGFQEA PLAYDAIWAL 370 380 390 400 410 420 ALALNKTSGG GGRSGVRLED FNYNNQTITD QIYRAMNSSS FEGVSGHVVF DASGSRMAWT 430 440 450 460 470 480 LIEQLQGGSY KKIGYYDSTK DDLSWSKTDK WIGGSPPADQ TLVIKTFRFL SQKLFISVSV 490 500 510 520 530 540 LSSLGIVLAV VCLSFNIYNS HVRYIQNSQP NLNNLTAVGC SLALAAVFPL GLDGYHIGRS 550 560 570 580 590 600 QFPFVCQARL WLLGLGFSLG YGSMFTKIWW VHTVFTKKEE KKEWRKTLEP WKLYATVGLL 610 620 630 640 650 660 VGMDVLTLAI WQIVDPLHRT IETFAKEEPK EDIDVSILPQ LEHCSSKKMN TWLGELWSFA 670 680 690 700 710 720 VSSDVQRRAT VGGDSPICVW PAPESIFYGY KGLLLLLGIF LAYETKSVST EKINDHRAVG 730 740 750 760 770 780 MAIYNVAVLC LITAPVTMIL SSQQDAAFAF ASLAIVFSSY ITLVVLFVPK MRRLITRGEW 790 800 810 820 830 840 QSETQDTMKT GSSTNNNEEE KSRLLEKENR ELEKIIAEKE ERVSELRHQL QSRQQLRSRR 850 860 870 HPPTPPDPSG GLPRGPSEPP DRLSCDGSRV HLLYK
	Isoform 1D [UniParc]. Checksum: 5EFD7B169A727FDE	FASTA	812	90,921
	10 20 30 40 50 60 MGPGGPCTPV GWPLPLLLVM AAGVAPVWAS HSPHLPRPHP RVPPHPSSER RAVYIGALFP 70 80 90 100 110 120 MSGGWPGGQA CQPAVEMALE DVNSRRDILP DYELKLIHHD SKCDPGQATK YLYELLYNDP 130 140 150 160 170 180 IKIILMPGCS SVSTLVAEAA RMWNLIVLSY GSSSPALSNR QRFPTFFRTH PSATLHNPTR 190 200 210 220 230 240 VKLFEKWGWK KIATIQQTTE VFTSTLDDLE ERVKEAGIEI TFRQSFFSDP AVPVKNLKRQ 250 260 270 280 290 300 DARIIVGLFY ETEARKVFCE VYKERLFGKK YVWFLIGWYA DNWFKTYDPS INCTVEEMTE 310 320 330 340 350 360 AVEGHITTEI VMLNPANTRS ISNMTSQEFV EKLTKRLKRH PEETGGFQEA PLAYDAIWAL 370 380 390 400 410 420 ALALNKTSGG GGRSGVRLED FNYNNQTITD QIYRAMNSSS FEGVSGHVVF DASGSRMAWT 430 440 450 460 470 480 LIEQLQGGSY KKIGYYDSTK DDLSWSKTDK WIGGSPPADQ TLVIKTFRFL SQKLFISVSV 490 500 510 520 530 540 LSSLGIVLAV VCLSFNIYNS HVRYIQNSQP NLNNLTAVGC SLALAAVFPL GLDGYHIGRS 550 560 570 580 590 600 QFPFVCQARL WLLGLGFSLG YGSMFTKIWW VHTVFTKKEE KKEWRKTLEP WKLYATVGLL 610 620 630 640 650 660 VGMDVLTLAI WQIVDPLHRT IETFAKEEPK EDIDVSILPQ LEHCSSKKMN TWLGIFYGYK 670 680 690 700 710 720 GLLLLLGIFL AYETKSVSTE KINDHRAVGM AIYNVAVLCL ITAPVTMILS SQQDAAFAFA 730 740 750 760 770 780 SLAIVFSSYI TLVVLFVPKM RRLITRGEWQ SETQDTMKTG SSTNNNEEEK SRLLEKENRE 790 800 810 LEKIIAEVCG DKQPGPPVSE GGLPVVGPSI EV

References

[1]	"Expression cloning of GABA(B) receptors uncovers similarity to metabotropic glutamate receptors." Kaupmann K., Huggel K., Heid J., Flor P.J., Bischoff S., Mickel S.J., McMaster G., Angst C., Bittiger H., Froestl W., Bettler B. Nature 386:239-246(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1A AND 1B). Strain: RICO. Tissue: Brain cortex and Cerebellum.
[2]	"Cloning and tissue distribution of novel splice variants of the rat GABAB receptor." Isomoto S., Kaibara M., Sakurai-Yamashita Y., Nagayama Y., Uezono Y., Yano K., Taniyama K. Biochem. Biophys. Res. Commun. 253:10-15(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1C AND 1D). Tissue: Cerebellum.
[3]	"Alternative splicing generates a novel isoform of the rat metabotropic GABA(B)R1 receptor." Pfaff T., Malitschek B., Kaupmann K., Prezeau L., Pin J.-P., Bettler B., Karschin A. Eur. J. Neurosci. 11:2874-2882(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORM 1E). Strain: Wistar. Tissue: Hippocampus.
[4]	"Identification of a GABAB receptor subunit, gb2, required for functional GABAB receptor activity." Ng G.Y.K., Clark J., Coulombe N., Ethier N., Hebert T.E., Sullivan R., Kargman S., Chateauneuf A., Tsukamoto N., McDonald T., Whiting P., Mezey E., Johnson M.P., Liu Q., Kolakowski L.F. Jr., Evans J.F., Bonner T.I., O'Neill G.P. J. Biol. Chem. 274:7607-7610(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1A), INTERACTION WITH GABBR2. Tissue: Brain.
[5]	"Synthesis of the nanomolar photoaffinity GABA(B) receptor ligand CGP 71872 reveals diversity in the tissue distribution of GABA(B) receptor forms." Belley M., Sullivan R., Reeves A., Evans J.F., O'Neill G.P., Ng G.Y.K. Bioorg. Med. Chem. 7:2697-2704(1999) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[6]	"Role of heteromer formation in GABAB receptor function." Kuner R., Koehr G., Gruenewald S., Eisenhardt G., Bach A., Kornau H.-C. Science 283:74-77(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GABBR2.
[7]	"The metabotropic GABAB receptor directly interacts with the activating transcription factor 4." Nehring R.B., Horikawa H.P.M., El Far O., Kneussel M., Brandstatter J.H., Stamm S., Wischmeyer E., Betz H., Karschin A. J. Biol. Chem. 275:35185-35191(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ATF4, SUBCELLULAR LOCATION.
[8]	"Ca(2+) requirement for high-affinity gamma-aminobutyric acid (GABA) binding at GABA(B) receptors: involvement of serine 269 of the GABA(B)R1 subunit." Galvez T., Urwyler S., Prezeau L., Mosbacher J., Joly C., Malitschek B., Heid J., Brabet I., Froestl W., Bettler B., Kaupmann K., Pin J.-P. Mol. Pharmacol. 57:419-426(2000) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF SER-247; SER-268 AND SER-269.
[9]	"Marlin-1, a novel RNA-binding protein associates with GABA receptors." Couve A., Restituito S., Brandon J.M., Charles K.J., Bawagan H., Freeman K.B., Pangalos M.N., Calver A.R., Moss S.J. J. Biol. Chem. 279:13934-13943(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH JAKMIP1.
[10]	"Structural analysis of the complement control protein (CCP) modules of GABA(B) receptor 1a: only one of the two CCP modules is compactly folded." Blein S., Ginham R., Uhrin D., Smith B.O., Soares D.C., Veltel S., McIlhinney R.A., White J.H., Barlow P.N. J. Biol. Chem. 279:48292-48306(2004) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 96-159, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y10369 mRNA. Translation: CAA71398.1.
Y10370 mRNA. Translation: CAA71399.1.
AB016160 mRNA. Translation: BAA34708.1.
AB016161 mRNA. Translation: BAA34709.1.
AF110797, AF110796 Genomic DNA. Translation: AAD19656.1.
AF110797, AF110796 Genomic DNA. Translation: AAD19657.1.
AF110797, AF110796 Genomic DNA. Translation: AAD19658.1.
AF110797, AF110796 Genomic DNA. Translation: AAD19659.1.

IPI

IPI00208182.
IPI00231464.
IPI00231465.
IPI00231466.
IPI00287081.

RefSeq

NP_112290.2. NM_031028.3.

UniGene

Rn.30059.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SRZ	NMR	-	A	96-159	[»]
1SS2	NMR	-	A	96-159	[»]

DisProt

DP00463.

ProteinModelPortal

Q9Z0U4.

SMR

Q9Z0U4. Positions 96-159.

ModBase

Search...

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

Q9Z0U4.

Proteomic databases

PaxDb

Q9Z0U4.

PRIDE

Q9Z0U4.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

81657.

KEGG

rno:81657.

UCSC

RGD:621537. rat.

Organism-specific databases

CTD

2550.

RGD

621537. Gabbr1.

Phylogenomic databases

eggNOG

COG0683.

HOGENOM

HOG000016575.

HOVERGEN

HBG051688.

InParanoid

Q9Z0U4.

KO

K04615.

OrthoDB

EOG4BG8VH.

Gene expression databases

ArrayExpress

Q9Z0U4.

Genevestigator

Q9Z0U4.

GermOnline

ENSRNOG00000000774. Rattus norvegicus.

Family and domain databases

InterPro

IPR001828. ANF_lig-bd_rcpt.
IPR017978. GPCR_3_C.
IPR002455. GPCR_3_GABA_rcpt_B.
IPR002456. GPCR_3_GABA_rcpt_B1.
IPR000436. Sushi_SCR_CCP.
[Graphical view]

Pfam

PF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF00084. Sushi. 2 hits.
[Graphical view]

PRINTS

PR01177. GABAB1RECPTR.
PR01176. GABABRECEPTR.

SMART

SM00032. CCP. 2 hits.
[Graphical view]

SUPFAM

SSF57535. Complement_control_module. 2 hits.

PROSITE

PS00979. G_PROTEIN_RECEP_F3_1. False negative.
PS00980. G_PROTEIN_RECEP_F3_2. False negative.
PS00981. G_PROTEIN_RECEP_F3_3. False negative.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q9Z0U4.

ChEMBL

CHEMBL2753.

EvolutionaryTrace

Q9Z0U4.

NextBio

615214.

Entry information

Entry name

GABR1_RAT

Accession

Primary (citable) accession number: Q9Z0U4
Secondary accession number(s): O08620 , O08621, Q9Z0F9, Q9Z308

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 125 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program