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Protein

Tight junction protein ZO-2

Gene

Tjp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in tight junctions and adherens junctions.

GO - Molecular functioni

GO - Biological processi

  • establishment of endothelial intestinal barrier Source: MGI
  • intestinal absorption Source: MGI
  • negative regulation of osteoclast development Source: MGI
  • regulation of membrane permeability Source: MGI
  • TNFSF11-mediated signaling pathway Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2028269. Signaling by Hippo.

Names & Taxonomyi

Protein namesi
Recommended name:
Tight junction protein ZO-2
Alternative name(s):
Tight junction protein 2
Zona occludens protein 2
Zonula occludens protein 2
Gene namesi
Name:Tjp2
Synonyms:Zo2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1341872. Tjp2.

Subcellular locationi

GO - Cellular componenti

  • bicellular tight junction Source: MGI
  • cell-cell adherens junction Source: MGI
  • cell-cell junction Source: MGI
  • cell junction Source: MGI
  • cell surface Source: MGI
  • cytoplasm Source: MGI
  • gap junction Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Nucleus, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000945441 – 1167Tight junction protein ZO-2Add BLAST1167

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei107PhosphoserineCombined sources1
Modified residuei127PhosphoserineBy similarity1
Modified residuei130PhosphoserineBy similarity1
Modified residuei140PhosphoserineCombined sources1
Modified residuei145PhosphoserineBy similarity1
Modified residuei147PhosphoserineBy similarity1
Modified residuei173PhosphoserineBy similarity1
Modified residuei194PhosphoserineBy similarity1
Modified residuei205PhosphoserineBy similarity1
Modified residuei239PhosphoserineCombined sources1
Modified residuei305PhosphoserineBy similarity1
Modified residuei378PhosphoserineCombined sources1
Modified residuei380PhosphoserineCombined sources1
Modified residuei386PhosphoserineBy similarity1
Modified residuei395PhosphoserineBy similarity1
Modified residuei404PhosphoserineBy similarity1
Modified residuei410PhosphoserineBy similarity1
Modified residuei411PhosphoserineBy similarity1
Modified residuei435PhosphothreonineBy similarity1
Modified residuei479PhosphoserineCombined sources1
Modified residuei554PhosphotyrosineCombined sources1
Modified residuei684PhosphoserineCombined sources1
Modified residuei884PhosphoserineCombined sources1
Modified residuei887PhosphothreonineCombined sources1
Modified residuei895PhosphoserineCombined sources1
Modified residuei902PhosphoserineBy similarity1
Modified residuei907PhosphothreonineBy similarity1
Modified residuei915PhosphothreonineBy similarity1
Modified residuei948PhosphoserineBy similarity1
Modified residuei960PhosphoserineBy similarity1
Modified residuei968PhosphoserineCombined sources1
Modified residuei988PhosphoserineCombined sources1
Modified residuei1044PhosphoserineBy similarity1
Modified residuei1095PhosphotyrosineCombined sources1
Modified residuei1124PhosphoserineBy similarity1
Modified residuei1136PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z0U1.
MaxQBiQ9Z0U1.
PaxDbiQ9Z0U1.
PeptideAtlasiQ9Z0U1.
PRIDEiQ9Z0U1.

PTM databases

iPTMnetiQ9Z0U1.
PhosphoSitePlusiQ9Z0U1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024812.
CleanExiMM_TJP2.
GenevisibleiQ9Z0U1. MM.

Interactioni

Subunit structurei

Homodimer, and heterodimer with ZO1. Interacts with UBN1. Interacts with SCRIB (By similarity). Interacts with occludin and SAFB. Interaction with SAFB occurs in the nucleus. Interacts with USP53 (via the C-terminal region) (PubMed:26609154).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204210. 4 interactors.
IntActiQ9Z0U1. 4 interactors.
MINTiMINT-113156.
STRINGi10090.ENSMUSP00000097154.

Structurei

Secondary structure

11167
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 14Combined sources11
Beta strandi17 – 19Combined sources3
Beta strandi23 – 26Combined sources4
Beta strandi42 – 46Combined sources5
Helixi52 – 55Combined sources4
Beta strandi61 – 67Combined sources7
Helixi75 – 84Combined sources10
Beta strandi87 – 98Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CSJNMR-A1-104[»]
ProteinModelPortaliQ9Z0U1.
SMRiQ9Z0U1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Z0U1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 97PDZ 1PROSITE-ProRule annotationAdd BLAST88
Domaini287 – 365PDZ 2PROSITE-ProRule annotationAdd BLAST79
Domaini489 – 570PDZ 3PROSITE-ProRule annotationAdd BLAST82
Domaini584 – 649SH3PROSITE-ProRule annotationAdd BLAST66
Domaini660 – 858Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST199

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1165 – 1167Interaction with SCRIBBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1139 – 1142Poly-Glu4

Sequence similaritiesi

Belongs to the MAGUK family.Curated
Contains 1 guanylate kinase-like domain.PROSITE-ProRule annotation
Contains 3 PDZ (DHR) domains.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG3580. Eukaryota.
ENOG410XQP3. LUCA.
GeneTreeiENSGT00840000129793.
HOGENOMiHOG000230923.
HOVERGENiHBG017627.
InParanoidiQ9Z0U1.
KOiK06098.
OMAiPEPRAQM.
OrthoDBiEOG091G0AJZ.
PhylomeDBiQ9Z0U1.
TreeFamiTF315957.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
3.40.50.300. 2 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR005417. ZO.
IPR005419. ZO-2.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 3 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR01597. ZONOCCLUDNS.
PR01599. ZONOCCLUDNS2.
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z0U1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEVIWEQYT VTLQKDSKRG FGIAVSGGRD NPHFENGETS IVISDVLPGG
60 70 80 90 100
PADGLLQEND RVVMVNGTPM EDVLHSFAVQ QLRKSGKIAA IVVKRPRKVQ
110 120 130 140 150
VAPLQGSPPL SHDDRGFEVI EEFDGRSFRS GYSERSRHSS HDMLSHSWEG
160 170 180 190 200
NRERGRPHQR TQSRERERSR GRSLERGLDQ EDYGRSRERS RGRSLERGLD
210 220 230 240 250
RDFVSRDHSR GRSIDRDYDR DYERSYHEAY EPDYGGGYSP SYDRRAHPET
260 270 280 290 300
RYERSRSREH LRSRSPSPES RSRHEHKGQH DPDRPIGVLL TKSKANEEYG
310 320 330 340 350
LRLGSQIFIK EMTRTGLATK DGNLHEGDII LKINGTVTEN MSLTDARKLI
360 370 380 390 400
EKSRGKLQLV VLRDSKQTLI NIPALNDSDS EVEDISEIES NRSFSPEERR
410 420 430 440 450
QQYSDQDYHS STEKLKERPS SREETSGRLS RMGATPTPFK STGDITAAGV
460 470 480 490 500
TEASREPRYQ EEGPVPQPRT APRVFLRPSP EDEAIYGPNT KMVRFKKGDS
510 520 530 540 550
VGLRLAGGND VGIFVAGIQE GTSAEQEGLQ EGDQILKVNT QDFRGLVRED
560 570 580 590 600
AVLYLLEIPK GETVTILAQS RADVYRDILA CGRGDSFFIR SHFECEKETP
610 620 630 640 650
QSLAFTRGEV FRVVDTLYDG KLGHWLAVRI GNELEKGLIP NKSRAEQMAS
660 670 680 690 700
VQNAQRENAG DRADFWRMRG QRSSGGVKKN LRKSREDLAA AVSVSTKFPA
710 720 730 740 750
YEKVLLREAG FKRPVVLFGP IADIAMERLA TELPDLFQTA KTEPKDAGSE
760 770 780 790 800
KSSGVVRLNT VRQIIEQDKH ALLDVTPKAV DLLNYTQWFP IVIFFNPDSR
810 820 830 840 850
QGVKTIRQRL SPTSNKSSRK LFDQANKLKK TCSHLFTATI NVNSANDGWF
860 870 880 890 900
GSLKDSIQQQ QNEAVWVSEG KMEGMDDDAE DRMSYLTAMG ADYLSCDSRL
910 920 930 940 950
ISDFEDTDGE GGAYTDNELE EPAEEPLVSS ITRSSEPVQH EENIRKSSPE
960 970 980 990 1000
PRAQMRRAAS RDQLRDASPP PAFKPEPPKA RSQNREDSFD YSKSNLPATA
1010 1020 1030 1040 1050
GSEIPGGSTK GYPPPIAAKP AFGRPILKPS TPVPMPESEE VGESTEEQED
1060 1070 1080 1090 1100
APRSVLGRVK IFEKMDHKAK LQRMQELQEA QNARIEIAQK HPDIYAVPIK
1110 1120 1130 1140 1150
APKPDAGLPP HMSSRPPEPQ KAPSRLYQDT RGSYGSDPEE EEYRQQLAAH
1160
SKRGYYSQPS RYRDTEL
Length:1,167
Mass (Da):131,280
Last modified:May 2, 2006 - v2
Checksum:iC29AF94F423A3A70
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100Q → L in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti105 – 107QGS → LGC in AAD19964 (PubMed:10026224).Curated3
Sequence conflicti111 – 112SH → CL in AAD19964 (PubMed:10026224).Curated2
Sequence conflicti156R → G in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti292K → Q in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti378S → R in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti447A → P in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti454S → T in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti470T → A in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti506A → P in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti633E → D in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti649A → D in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti664D → V in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti667R → W in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti670G → R in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti674S → R in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti677V → D in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti680N → T in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti690 – 691AA → RS in AAD19964 (PubMed:10026224).Curated2
Sequence conflicti731T → N in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti1035M → I in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti1038S → T in AAD19964 (PubMed:10026224).Curated1
Sequence conflicti1069A → P in AAD19964 (PubMed:10026224).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113005 mRNA. Translation: AAD19964.1.
BC034677 mRNA. Translation: AAH34677.1.
BC039924 mRNA. Translation: AAH39924.1.
CCDSiCCDS37939.1.
RefSeqiNP_035727.2. NM_011597.4.
XP_006526971.1. XM_006526908.3.
XP_011245521.1. XM_011247219.2.
UniGeneiMm.104744.

Genome annotation databases

EnsembliENSMUST00000099558; ENSMUSP00000097154; ENSMUSG00000024812.
GeneIDi21873.
KEGGimmu:21873.
UCSCiuc008ham.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113005 mRNA. Translation: AAD19964.1.
BC034677 mRNA. Translation: AAH34677.1.
BC039924 mRNA. Translation: AAH39924.1.
CCDSiCCDS37939.1.
RefSeqiNP_035727.2. NM_011597.4.
XP_006526971.1. XM_006526908.3.
XP_011245521.1. XM_011247219.2.
UniGeneiMm.104744.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CSJNMR-A1-104[»]
ProteinModelPortaliQ9Z0U1.
SMRiQ9Z0U1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204210. 4 interactors.
IntActiQ9Z0U1. 4 interactors.
MINTiMINT-113156.
STRINGi10090.ENSMUSP00000097154.

PTM databases

iPTMnetiQ9Z0U1.
PhosphoSitePlusiQ9Z0U1.

Proteomic databases

EPDiQ9Z0U1.
MaxQBiQ9Z0U1.
PaxDbiQ9Z0U1.
PeptideAtlasiQ9Z0U1.
PRIDEiQ9Z0U1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099558; ENSMUSP00000097154; ENSMUSG00000024812.
GeneIDi21873.
KEGGimmu:21873.
UCSCiuc008ham.2. mouse.

Organism-specific databases

CTDi9414.
MGIiMGI:1341872. Tjp2.

Phylogenomic databases

eggNOGiKOG3580. Eukaryota.
ENOG410XQP3. LUCA.
GeneTreeiENSGT00840000129793.
HOGENOMiHOG000230923.
HOVERGENiHBG017627.
InParanoidiQ9Z0U1.
KOiK06098.
OMAiPEPRAQM.
OrthoDBiEOG091G0AJZ.
PhylomeDBiQ9Z0U1.
TreeFamiTF315957.

Enzyme and pathway databases

ReactomeiR-MMU-2028269. Signaling by Hippo.

Miscellaneous databases

EvolutionaryTraceiQ9Z0U1.
PROiQ9Z0U1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024812.
CleanExiMM_TJP2.
GenevisibleiQ9Z0U1. MM.

Family and domain databases

Gene3Di2.30.42.10. 3 hits.
3.40.50.300. 2 hits.
InterProiIPR008145. GK/Ca_channel_bsu.
IPR008144. Guanylate_kin-like_dom.
IPR027417. P-loop_NTPase.
IPR001478. PDZ.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR005417. ZO.
IPR005419. ZO-2.
[Graphical view]
PfamiPF00625. Guanylate_kin. 1 hit.
PF00595. PDZ. 3 hits.
PF07653. SH3_2. 1 hit.
[Graphical view]
PRINTSiPR01597. ZONOCCLUDNS.
PR01599. ZONOCCLUDNS2.
SMARTiSM00072. GuKc. 1 hit.
SM00228. PDZ. 3 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50156. SSF50156. 3 hits.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50052. GUANYLATE_KINASE_2. 1 hit.
PS50106. PDZ. 3 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZO2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0U1
Secondary accession number(s): Q8K210
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 2, 2006
Last modified: November 2, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.