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Protein

Thiopurine S-methyltransferase

Gene

Tpmt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.

Catalytic activityi

S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35SubstrateBy similarity1
Binding sitei64S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei85S-adenosyl-L-methionineBy similarity1
Binding sitei147S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

GO - Biological processi

  • response to organic cyclic compound Source: RGD
  • response to testosterone Source: RGD

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-RNO-156581. Methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiopurine S-methyltransferase (EC:2.1.1.67)
Alternative name(s):
Thiopurine methyltransferase
Gene namesi
Name:Tpmt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi1585162. Tpmt.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002201131 – 240Thiopurine S-methyltransferaseAdd BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei53N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ9Z0T0.

PTM databases

iPTMnetiQ9Z0T0.
PhosphoSitePlusiQ9Z0T0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016468.
ExpressionAtlasiQ9Z0T0. baseline and differential.
GenevisibleiQ9Z0T0. RN.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9Z0T0.
SMRiQ9Z0T0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 35S-adenosyl-L-methionine bindingBy similarityAdd BLAST12
Regioni129 – 130S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016823.
HOVERGENiHBG003037.
PhylomeDBiQ9Z0T0.

Family and domain databases

HAMAPiMF_00812. Thiopur_methtran. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025835. Thiopurine_S-MeTrfase.
IPR008854. TPMT.
PANTHERiPTHR10259. PTHR10259. 1 hit.
PfamiView protein in Pfam
PF05724. TPMT. 1 hit.
PIRSFiPIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS51585. SAM_MT_TPMT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z0T0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLGIKEHPD AEVQKNRVLT LDDWQDKWVT RHIAFHQEQG HQLLKKHFDT
60 70 80 90 100
FLKGQSGLRV FFPLCGKAIE MKWFADRGHT VVGVEISEIG IREFFAEQNL
110 120 130 140 150
SYTEEPLTEI AGAKVFKSSS GNISLYCCSI FDLPRANIGK FDRIWDRGAL
160 170 180 190 200
VAVNPGDRDR YADIILSLLR KGYHYLLVVL SYDPTKHTGP PFYVPDAELK
210 220 230 240
KLFGTKCNMQ CLEEVDALEE RHKTWGVDYF FEKLYLLTEK
Length:240
Mass (Da):27,692
Last modified:May 1, 1999 - v1
Checksum:i04608B546BBDC1E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF120100 mRNA. Translation: AAD17293.1.
RefSeqiXP_006253832.1. XM_006253770.3.
UniGeneiRn.112598.

Genome annotation databases

EnsembliENSRNOT00000022085; ENSRNOP00000022084; ENSRNOG00000016468.
GeneIDi690050.
UCSCiRGD:620089. rat.

Similar proteinsi

Entry informationi

Entry nameiTPMT_RAT
AccessioniPrimary (citable) accession number: Q9Z0T0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 1999
Last modified: August 30, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families