Reviewed, UniProtKB/Swiss-Prot Q9Z0T0 (TPMT_RAT)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:

Tpmt

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	S-adenosylhomocysteine metabolic process Inferred from direct assay. Source: RGD S-adenosylmethionine metabolic process Inferred from direct assay. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD tight junction Non-traceable author statement. Source: RGD
Molecular function	thiopurine S-methyltransferase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Thiopurine S-methyltransferase		PRO_0000220113
Sites
	Binding site	28	1	S-adenosyl-L-methionine By similarity
	Binding site	64	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	85	1	S-adenosyl-L-methionine By similarity
	Binding site	147	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z0T0-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 04608B546BBDC1E3	FASTA	240	27,692
	10 20 30 40 50 60 MSLGIKEHPD AEVQKNRVLT LDDWQDKWVT RHIAFHQEQG HQLLKKHFDT FLKGQSGLRV 70 80 90 100 110 120 FFPLCGKAIE MKWFADRGHT VVGVEISEIG IREFFAEQNL SYTEEPLTEI AGAKVFKSSS 130 140 150 160 170 180 GNISLYCCSI FDLPRANIGK FDRIWDRGAL VAVNPGDRDR YADIILSLLR KGYHYLLVVL 190 200 210 220 230 240 SYDPTKHTGP PFYVPDAELK KLFGTKCNMQ CLEEVDALEE RHKTWGVDYF FEKLYLLTEK

References

[1]	Krynetski E.Y., Fessing M.Y., Evans W.E. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF120100 mRNA. Translation: AAD17293.1.
IPI	IPI00208135.
RefSeq	XP_001058187.1.
UniGene	Rn.112598
3D structure databases
SMR	Q9Z0T0. Positions 10-240.
ModBase	Search...
Proteomic databases
PRIDE	Q9Z0T0.
Genome annotation databases
GeneID	681730.
KEGG	rno:681730.
Organism-specific databases
RGD	620089. Tpmt.
Phylogenomic databases
HOVERGEN	Q9Z0T0.
Enzyme and pathway databases
BRENDA	2.1.1.67. 248.
Family and domain databases
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	721509.

Entry information

Entry name

TPMT_RAT

Accession

Primary (citable) accession number: Q9Z0T0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)