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Protein

Prenylated Rab acceptor protein 1

Gene

Rabac1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition it inhibits the removal of Rab GTPases from the membrane by GDI1 (By similarity).By similarity1 Publication

GO - Molecular functioni

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Prenylated Rab acceptor protein 1
Alternative name(s):
PRA1 family protein 1
Prenylin
Gene namesi
Name:Rabac1
Synonyms:Pra, Pra1, Praf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1201692. Rabac1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
  • Cytoplasm By similarity
  • Golgi apparatus By similarity
  • Cytoplasmic vesiclesecretory vesiclesynaptic vesicle By similarity

  • Note: According to PubMed:11535589, it is an integral membrane protein, while other authors showed that it is cytoplasmic and membrane-associated to Golgi and synaptic vesicles.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 78Cytoplasmic1 PublicationAdd BLAST78
Transmembranei79 – 94HelicalAdd BLAST16
Transmembranei95 – 112HelicalAdd BLAST18
Topological domaini113 – 131Cytoplasmic1 PublicationAdd BLAST19
Transmembranei132 – 148HelicalAdd BLAST17
Transmembranei149 – 165HelicalAdd BLAST17
Topological domaini166 – 185Cytoplasmic1 PublicationAdd BLAST20

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • Golgi apparatus Source: MGI
  • integral component of membrane Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
  • synaptic vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176D → A: No effect. 1 Publication1
Mutagenesisi177G → A: No effect. 1 Publication1
Mutagenesisi178E → A: No effect. 1 Publication1
Mutagenesisi179E → A: No effect. 1 Publication1
Mutagenesisi185V → A, C, I or P: Partial retention in the endoplasmic reticulum. 1 Publication1
Mutagenesisi185V → D or K: Retention in the endoplasmic reticulum; does not form homodimers. 1 Publication1
Mutagenesisi185V → M, N, Y, H, E, Q, T, S, L, W, F or G: Retention in the endoplasmic reticulum. 1 Publication1
Mutagenesisi185V → R: No effect. 1 Publication1
Mutagenesisi185Missing : Partial retention in the endoplasmic reticulum; still forms homodimers. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002208791 – 185Prenylated Rab acceptor protein 1Add BLAST185

Proteomic databases

EPDiQ9Z0S9.
MaxQBiQ9Z0S9.
PaxDbiQ9Z0S9.
PeptideAtlasiQ9Z0S9.
PRIDEiQ9Z0S9.

PTM databases

iPTMnetiQ9Z0S9.
PhosphoSitePlusiQ9Z0S9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000003380.
CleanExiMM_RABAC1.
ExpressionAtlasiQ9Z0S9. baseline and differential.
GenevisibleiQ9Z0S9. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with VAMP2 (synaptobrevin-2), GDI1, NRDG1 and PCLO (By similarity). Interacts with prenylated Rab proteins (including RAB5 and RAB6), and with the members of the Ras superfamily HRAS, RHOA, TC21, and RAP1A.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HRASP011124EBI-476965,EBI-350145From a different organism.
RHOAP615863EBI-476965,EBI-446668From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199848. 1 interactor.
IntActiQ9Z0S9. 5 interactors.
STRINGi10090.ENSMUSP00000076227.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0S9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 54Required for interaction with prenylated RAB3A and VAMP2By similarityAdd BLAST25
Regioni165 – 185Required for interaction with GDI1By similarityAdd BLAST21
Regioni175 – 185HomodimerizationAdd BLAST11
Regioni175 – 185Required for interaction with prenylated RAB3A and VAMP2By similarityAdd BLAST11

Sequence similaritiesi

Belongs to the PRA1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3142. Eukaryota.
COG5130. LUCA.
GeneTreeiENSGT00390000010549.
HOGENOMiHOG000230621.
HOVERGENiHBG053660.
InParanoidiQ9Z0S9.
KOiK20359.
OMAiATIRSWG.
OrthoDBiEOG091G0M2S.
PhylomeDBiQ9Z0S9.
TreeFamiTF324857.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z0S9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQKDQQKD AEGEGLSATT LLPKLIPSGA GREWLERRRA TIRPWGTFVD
60 70 80 90 100
QQRFSRPRNV GELCQRLVRN VEYYQSNYVF VFLGLILYCV VTSPMLLVAL
110 120 130 140 150
AVFFGACYIL YLRTLQSKLV LFGREVSPAH QYALAGGVSF PFFWLAGAGS
160 170 180
AVFWVLGATL VLIGSHAAFH QMEPADGEEL QMEPV
Length:185
Mass (Da):20,619
Last modified:May 1, 1999 - v1
Checksum:i86C82C0502B5CB5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF252856 mRNA. Translation: AAF68476.1.
AF120162 mRNA. Translation: AAD17296.1.
AK008559 mRNA. Translation: BAB25744.1.
AK009726 mRNA. Translation: BAB26465.1.
AK150681 mRNA. Translation: BAE29761.1.
AK170289 mRNA. Translation: BAE41689.1.
BC008242 mRNA. Translation: AAH08242.1.
CCDSiCCDS20968.1.
PIRiJC7369.
RefSeqiNP_034391.1. NM_010261.2.
UniGeneiMm.22473.

Genome annotation databases

EnsembliENSMUST00000076961; ENSMUSP00000076227; ENSMUSG00000003380.
GeneIDi14470.
KEGGimmu:14470.
UCSCiuc009frd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF252856 mRNA. Translation: AAF68476.1.
AF120162 mRNA. Translation: AAD17296.1.
AK008559 mRNA. Translation: BAB25744.1.
AK009726 mRNA. Translation: BAB26465.1.
AK150681 mRNA. Translation: BAE29761.1.
AK170289 mRNA. Translation: BAE41689.1.
BC008242 mRNA. Translation: AAH08242.1.
CCDSiCCDS20968.1.
PIRiJC7369.
RefSeqiNP_034391.1. NM_010261.2.
UniGeneiMm.22473.

3D structure databases

ProteinModelPortaliQ9Z0S9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199848. 1 interactor.
IntActiQ9Z0S9. 5 interactors.
STRINGi10090.ENSMUSP00000076227.

PTM databases

iPTMnetiQ9Z0S9.
PhosphoSitePlusiQ9Z0S9.

Proteomic databases

EPDiQ9Z0S9.
MaxQBiQ9Z0S9.
PaxDbiQ9Z0S9.
PeptideAtlasiQ9Z0S9.
PRIDEiQ9Z0S9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076961; ENSMUSP00000076227; ENSMUSG00000003380.
GeneIDi14470.
KEGGimmu:14470.
UCSCiuc009frd.1. mouse.

Organism-specific databases

CTDi10567.
MGIiMGI:1201692. Rabac1.

Phylogenomic databases

eggNOGiKOG3142. Eukaryota.
COG5130. LUCA.
GeneTreeiENSGT00390000010549.
HOGENOMiHOG000230621.
HOVERGENiHBG053660.
InParanoidiQ9Z0S9.
KOiK20359.
OMAiATIRSWG.
OrthoDBiEOG091G0M2S.
PhylomeDBiQ9Z0S9.
TreeFamiTF324857.

Miscellaneous databases

PROiQ9Z0S9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003380.
CleanExiMM_RABAC1.
ExpressionAtlasiQ9Z0S9. baseline and differential.
GenevisibleiQ9Z0S9. MM.

Family and domain databases

InterProiIPR004895. Prenylated_rab_accept_PRA1.
[Graphical view]
PfamiPF03208. PRA1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRAF1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0S9
Secondary accession number(s): Q3TDB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Interaction with the Ras-like GTPases failed to be confirmed in other species.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.