Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Claudin-10

Gene

Cldn10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. May form permselective paracellular pores; isoform 1 appears to create pores preferentially permeable to cations and isoform 2 for anions. Plays a key role in controlling cation selectivity and transport in the thick ascending limb (TAL) of Henle's loop in kidney.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Protein family/group databases

TCDBi1.H.1.1.10. the claudin tight junction (claudin1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Claudin-10
Gene namesi
Name:Cldn10
Synonyms:Cldn10a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1913101. Cldn10.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21
Topological domaini22 – 80ExtracellularSequence analysisAdd BLAST59
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Topological domaini102 – 115CytoplasmicSequence analysisAdd BLAST14
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Topological domaini137 – 160ExtracellularSequence analysisAdd BLAST24
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Topological domaini182 – 231CytoplasmicSequence analysisAdd BLAST50

GO - Cellular componenti

  • bicellular tight junction Source: UniProtKB
  • cytoplasm Source: MGI
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Endoplasmic reticulum, Membrane, Tight junction

Pathology & Biotechi

Disruption phenotypei

Conditional knockout in the thick ascending limb (TAL) of Henle's loop in kidney leads to hypocalcemia, hypermagnesemia, hyperphosphatemia and nephrocalcinosis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447581 – 231Claudin-10Add BLAST231

Proteomic databases

PaxDbiQ9Z0S6.
PRIDEiQ9Z0S6.

PTM databases

iPTMnetiQ9Z0S6.
PhosphoSitePlusiQ9Z0S6.

Expressioni

Tissue specificityi

Strong expression detected in brain cortex and kidney and weak expression in lung and cecum. In kidney, detected in thick ascending limb (TAL) of Henle's loop and proximal convoluted tubule (PCT). In inner ear, detected in organ of Corti, marginal cells of stria vascularis, Reissner's membrane and spiral limbus (at protein level). Isoform 1 is widely expressed, with highest expression detected in brain cortex, kidney and lung. Isoform 2, isoform 3, isoform 4 and isoform 5 are only detected in kidney and uterus. In kidney, the expression of isoform 1 is highest in medulla, with transcripts being detected in medullary thick ascending limb of Henle's loop (mTAL) and outer and inner medullary collecting ducts, whereas isoform 2 (along with isoform 4) is more highly expressed in cortex, with transcripts being detected in PCT, mTAL and cortical collecting duct.4 Publications

Developmental stagei

Detected in developing kidney at E14, with levels increasing towards adulthood. Expressed during tooth development: at E12, detected in the thickening tooth epithelium, at E13.5 in the lingual basal epithelium of the bud epithelium, at E14.5 in lingual epithelium and between E18 to postnatal day 0 in odontoblasts and stratum intermedium.2 Publications

Gene expression databases

BgeeiENSMUSG00000022132.
GenevisibleiQ9Z0S6. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-48952N.
DIP-48954N.
STRINGi10090.ENSMUSP00000097889.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0S6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The fourth transmembrane region (161-181), which is missing in isoform 3, isoform 5 and isoform 6, is necessary for integration into tight junctions.1 Publication

Sequence similaritiesi

Belongs to the claudin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQ7E. Eukaryota.
ENOG410YF7U. LUCA.
GeneTreeiENSGT00760000119222.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiQ9Z0S6.
KOiK06087.
OMAiPRMGYAY.
OrthoDBiEOG091G0MX2.
TreeFamiTF331936.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003554. Claudin10.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01383. CLAUDIN10.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z0S6-1) [UniParc]FASTAAdd to basket
Also known as: Cldn10b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTALEIVA FVVSISGWVL VSSTLPTDYW KVSTIDGTVI TTATYFANLW
60 70 80 90 100
KICVTDSTGV ANCKEFPSML ALDGYIQACR GLMIAAVSLG FFGSIFALFG
110 120 130 140 150
MKCTKVGGSD QAKAKIACLA GIVFILSGLC SMTGCSLYAN KITTEFFDPL
160 170 180 190 200
YMEQKYELGA ALFIGWAGAS LCIIGGVIFC FSISDNNKTP RMGYTYNGPT
210 220 230
SAMSSRTKYQ GGEGDFKTTG PSKQFDKNAY V
Length:231
Mass (Da):24,695
Last modified:January 22, 2014 - v2
Checksum:i80A28863B258DED2
GO
Isoform 2 (identifier: Q9Z0S6-2) [UniParc]FASTAAdd to basket
Also known as: Cldn10a

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MASTALEIVA...KEFPSMLALD → MSRAQISALV...RPHFTIFKVE

Show »
Length:229
Mass (Da):24,451
Checksum:i3FF402779730858E
GO
Isoform 3 (identifier: Q9Z0S6-3) [UniParc]FASTAAdd to basket
Also known as: Cldn10a_i2

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MASTALEIVA...KEFPSMLALD → MSRAQISALV...RPHFTIFKVE
     156-191: Missing.

Show »
Length:193
Mass (Da):20,664
Checksum:iA55E86F351B7AE8C
GO
Isoform 4 (identifier: Q9Z0S6-4) [UniParc]FASTAAdd to basket
Also known as: Cldn10a_i1

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MASTALEIVA...KEFPSMLALD → MSRAQISALV...VYQGLWMNCA

Note: Produced by alternative splicing of isoform 2.
Show »
Length:210
Mass (Da):22,309
Checksum:i8BB45CC596FD9D3D
GO
Isoform 5 (identifier: Q9Z0S6-5) [UniParc]FASTAAdd to basket
Also known as: Cldn10a_i3

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MASTALEIVA...KEFPSMLALD → MSRAQISALV...VYQGLWMNCA
     156-191: Missing.

Note: Produced by alternative splicing of isoform 3.
Show »
Length:174
Mass (Da):18,521
Checksum:i597B914F3B16D98E
GO
Isoform 6 (identifier: Q9Z0S6-6) [UniParc]FASTAAdd to basket
Also known as: Cldn10b_i1

The sequence of this isoform differs from the canonical sequence as follows:
     156-191: Missing.

Show »
Length:195
Mass (Da):20,907
Checksum:i1103AEA5508A6DF7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti104T → S in BAB32005 (Ref. 1) Curated1
Sequence conflicti202A → V in AAD17320 (Ref. 1) Curated1
Sequence conflicti202A → V in AAH21770 (PubMed:15489334).Curated1
Sequence conflicti202A → V in AAH29019 (PubMed:15489334).Curated1
Sequence conflicti219T → A in AAD17320 (Ref. 1) Curated1
Sequence conflicti219T → A in AAH21770 (PubMed:15489334).Curated1
Sequence conflicti219T → A in AAH29019 (PubMed:15489334).Curated1
Sequence conflicti219T → A no nucleotide entry (PubMed:19383724).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0535511 – 73MASTA…MLALD → MSRAQISALVCGVGGFGALV AATTSNEWKVTTRASSVITA TWVYQGLWMNCAGNALGSFH CRPHFTIFKVE in isoform 2 and isoform 3. 4 PublicationsAdd BLAST73
Alternative sequenceiVSP_0535521 – 73MASTA…MLALD → MSRAQISALVCGVGGFGALV AATTSNEWKVTTRASSVITA TWVYQGLWMNCA in isoform 4 and isoform 5. 1 PublicationAdd BLAST73
Alternative sequenceiVSP_053553156 – 191Missing in isoform 3, isoform 5 and isoform 6. 2 PublicationsAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124425 mRNA. Translation: AAD17320.1.
AK020131 mRNA. Translation: BAB32005.1.
AC154377 Genomic DNA. No translation available.
CT025524 Genomic DNA. No translation available.
BC021770 mRNA. Translation: AAH21770.1.
BC029019 mRNA. Translation: AAH29019.1.
CCDSiCCDS37009.1. [Q9Z0S6-2]
CCDS37010.1. [Q9Z0S6-1]
CCDS49566.1. [Q9Z0S6-3]
RefSeqiNP_001153568.1. NM_001160096.1. [Q9Z0S6-4]
NP_001153569.1. NM_001160097.1. [Q9Z0S6-3]
NP_001153570.1. NM_001160098.1. [Q9Z0S6-5]
NP_001153571.1. NM_001160099.1. [Q9Z0S6-6]
NP_067361.2. NM_021386.4. [Q9Z0S6-1]
NP_076367.2. NM_023878.3. [Q9Z0S6-2]
UniGeneiMm.390755.

Genome annotation databases

EnsembliENSMUST00000047761; ENSMUSP00000041616; ENSMUSG00000022132. [Q9Z0S6-2]
ENSMUST00000071546; ENSMUSP00000071476; ENSMUSG00000022132. [Q9Z0S6-3]
ENSMUST00000100314; ENSMUSP00000097889; ENSMUSG00000022132. [Q9Z0S6-1]
GeneIDi58187.
KEGGimmu:58187.
UCSCiuc007uyw.2. mouse. [Q9Z0S6-2]
uc007uyx.2. mouse. [Q9Z0S6-1]
uc011zqb.1. mouse. [Q9Z0S6-4]
uc011zqc.1. mouse. [Q9Z0S6-5]
uc011zqd.1. mouse. [Q9Z0S6-3]
uc011zqe.1. mouse. [Q9Z0S6-6]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF124425 mRNA. Translation: AAD17320.1.
AK020131 mRNA. Translation: BAB32005.1.
AC154377 Genomic DNA. No translation available.
CT025524 Genomic DNA. No translation available.
BC021770 mRNA. Translation: AAH21770.1.
BC029019 mRNA. Translation: AAH29019.1.
CCDSiCCDS37009.1. [Q9Z0S6-2]
CCDS37010.1. [Q9Z0S6-1]
CCDS49566.1. [Q9Z0S6-3]
RefSeqiNP_001153568.1. NM_001160096.1. [Q9Z0S6-4]
NP_001153569.1. NM_001160097.1. [Q9Z0S6-3]
NP_001153570.1. NM_001160098.1. [Q9Z0S6-5]
NP_001153571.1. NM_001160099.1. [Q9Z0S6-6]
NP_067361.2. NM_021386.4. [Q9Z0S6-1]
NP_076367.2. NM_023878.3. [Q9Z0S6-2]
UniGeneiMm.390755.

3D structure databases

ProteinModelPortaliQ9Z0S6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48952N.
DIP-48954N.
STRINGi10090.ENSMUSP00000097889.

Protein family/group databases

TCDBi1.H.1.1.10. the claudin tight junction (claudin1) family.

PTM databases

iPTMnetiQ9Z0S6.
PhosphoSitePlusiQ9Z0S6.

Proteomic databases

PaxDbiQ9Z0S6.
PRIDEiQ9Z0S6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047761; ENSMUSP00000041616; ENSMUSG00000022132. [Q9Z0S6-2]
ENSMUST00000071546; ENSMUSP00000071476; ENSMUSG00000022132. [Q9Z0S6-3]
ENSMUST00000100314; ENSMUSP00000097889; ENSMUSG00000022132. [Q9Z0S6-1]
GeneIDi58187.
KEGGimmu:58187.
UCSCiuc007uyw.2. mouse. [Q9Z0S6-2]
uc007uyx.2. mouse. [Q9Z0S6-1]
uc011zqb.1. mouse. [Q9Z0S6-4]
uc011zqc.1. mouse. [Q9Z0S6-5]
uc011zqd.1. mouse. [Q9Z0S6-3]
uc011zqe.1. mouse. [Q9Z0S6-6]

Organism-specific databases

CTDi9071.
MGIiMGI:1913101. Cldn10.

Phylogenomic databases

eggNOGiENOG410IQ7E. Eukaryota.
ENOG410YF7U. LUCA.
GeneTreeiENSGT00760000119222.
HOGENOMiHOG000220937.
HOVERGENiHBG000643.
InParanoidiQ9Z0S6.
KOiK06087.
OMAiPRMGYAY.
OrthoDBiEOG091G0MX2.
TreeFamiTF331936.

Miscellaneous databases

PROiQ9Z0S6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022132.
GenevisibleiQ9Z0S6. MM.

Family and domain databases

InterProiIPR006187. Claudin.
IPR003554. Claudin10.
IPR017974. Claudin_CS.
IPR004031. PMP22/EMP/MP20/Claudin.
[Graphical view]
PANTHERiPTHR12002. PTHR12002. 1 hit.
PfamiPF00822. PMP22_Claudin. 1 hit.
[Graphical view]
PRINTSiPR01383. CLAUDIN10.
PROSITEiPS01346. CLAUDIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLD10_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0S6
Secondary accession number(s): E9PVC8
, E9PWP4, E9QMP1, Q8VC62, Q9CX57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 22, 2014
Last modified: November 2, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.