Q9Z0S2 (LHX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LIM/homeobox protein Lhx2
Short name=Homeobox protein LH-2
Short name=LIM homeobox protein 2

Gene names

Name:

Lhx2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types. Ref.3
Subunit structure	interacts (via LIM domains) with CITED2. Ref.3
Subcellular location	Nucleus Probable.
Domain	LIM domains are necessary for transcription activation.
Sequence similarities	Contains 1 homeobox DNA-binding domain. Contains 2 LIM zinc-binding domains.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox LIM domain Repeat
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon guidance Inferred from mutant phenotype PubMed 17493606. Source: MGI dorsal/ventral pattern formation Inferred from mutant phenotype PubMed 11165475PubMed 14566948. Source: MGI mesoderm development Inferred from mutant phenotype PubMed 9247336. Source: MGI negative regulation of transcription regulatory region DNA binding Inferred from genetic interaction PubMed 9697309. Source: MGI neural tube closure Inferred from mutant phenotype PubMed 11719201. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 10431247. Source: MGI telencephalon regionalization Inferred from mutant phenotype PubMed 11719201. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay PubMed 18076286. Source: MGI
Molecular_function	protein binding transcription factor activity Inferred from physical interaction PubMed 17991461. Source: MGI sequence-specific DNA binding Inferred from direct assay PubMed 15173589. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	LIM/homeobox protein Lhx2		PRO_0000075779
Regions
	Domain	53 – 105	53	LIM zinc-binding 1
	Domain	115 – 168	54	LIM zinc-binding 2
	DNA binding	266 – 325	60	Homeobox
	Motif	307 – 323	17	Nuclear localization signal Potential
	Compositional bias	187 – 196	10	Poly-Ala
Experimental info
	Mutagenesis	53	1	C → A: Reduces transcriptional activation; when associated with A-56. Ref.3
	Mutagenesis	56	1	C → A: Reduces transcriptional activation; when associated with A-53. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9Z0S2 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: FE7B4E76454D6A90	FASTA	406	44,419
	10 20 30 40 50 60 MLFHSLSGPE VHGVIDEMDR RAKSEAPAIS SAIDRGDTET TMPSISSDRA ALCAGCGGKI 70 80 90 100 110 120 SDRYYLLAVD KQWHMRCLKC CECKLNLESE LTCFSKDGSI YCKEDYYRRF SVQRCARCHL 130 140 150 160 170 180 GISASEMVMR ARDLVYHLNC FTCTTCNKML TTGDHFGMKD SLVYCRLHFE ALLQGEYPAH 190 200 210 220 230 240 FNHADVAAAA AAAAAAKSAG LGSAGANPLG LPYYNGVGTV QKGRPRKRKS PGPGADLAAY 250 260 270 280 290 300 NAALSCNEND AEHLDRDQPY PSSQKTKRMR TSFKHHQLRT MKSYFAINHN PDAKDLKQLA 310 320 330 340 350 360 QKTGLTKRVL QVWFQNARAK FRRNLLRQEN TGVDKTSDAT LQTGTPSGPA SELSNASLSP 370 380 390 400 SSTPTTLTDL TSPTLPTVTS VLTSVPGNLE GHEPHSPSQT TLTNLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conservation of the expression and function of apterous orthologs in Drosophila and mammals." Rincon-Limas D.E., Lu C.-H., Canal I., Calleja M., Rodriguez-Esteban C., Izpisua-Belmonte J.-C., Botas J. Proc. Natl. Acad. Sci. U.S.A. 96:2165-2170(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"MRG1 binds to the LIM domain of Lhx2 and may function as a coactivator to stimulate glycoprotein hormone alpha-subunit gene expression." Glenn D.J., Maurer R.A. J. Biol. Chem. 274:36159-36167(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CITED2, MUTAGENESIS OF CYS-53 AND CYS-56.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF124734 mRNA. Translation: AAD20012.1. BC055741 mRNA. Translation: AAH55741.1.
IPI	IPI00129385.
RefSeq	NP_034840.1. NM_010710.3.
UniGene	Mm.142856.
3D structure databases
ProteinModelPortal	Q9Z0S2.
SMR	Q9Z0S2. Positions 53-165, 262-337.
ModBase	Search...
PTM databases
PhosphoSite	Q9Z0S2.
Proteomic databases
PaxDb	Q9Z0S2.
PRIDE	Q9Z0S2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000000253; ENSMUSP00000000253; ENSMUSG00000000247.
GeneID	16870.
KEGG	mmu:16870.
Organism-specific databases
CTD	9355.
MGI	MGI:96785. Lhx2.
Phylogenomic databases
eggNOG	NOG240987.
GeneTree	ENSGT00680000099670.
HOGENOM	HOG000034022.
HOVERGEN	HBG006262.
InParanoid	Q9Z0S2.
KO	K09373.
OMA	CNENDAE.
OrthoDB	EOG4G4GQK.
Gene expression databases
ArrayExpress	Q9Z0S2.
Bgee	Q9Z0S2.
CleanEx	MM_LHX2.
Genevestigator	Q9Z0S2.
GermOnline	ENSMUSG00000000247. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit. 2.10.110.10. 2 hits.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR001781. Znf_LIM. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. PF00412. LIM. 2 hits. [Graphical view]
SMART	SM00389. HOX. 1 hit. SM00132. LIM. 2 hits. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. PS00478. LIM_DOMAIN_1. 2 hits. PS50023. LIM_DOMAIN_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	290834.
SOURCE	Search...

Entry information

Entry name

LHX2_MOUSE

Accession

Primary (citable) accession number: Q9Z0S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1999
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program