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Q9Z0S2 (LHX2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
LIM/homeobox protein Lhx2

Short name=Homeobox protein LH-2
Short name=LIM homeobox protein 2
Gene names
Name:Lhx2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types. Ref.3

Subunit structure

interacts (via LIM domains) with CITED2. Ref.3

Subcellular location

Nucleus Probable.

Domain

LIM domains are necessary for transcription activation.

Sequence similarities

Contains 1 homeobox DNA-binding domain.

Contains 2 LIM zinc-binding domains.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
LIM domain
Repeat
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanatomical structure formation involved in morphogenesis

Inferred from mutant phenotype PubMed 11719201. Source: MGI

axon guidance

Inferred from mutant phenotype PubMed 17493606. Source: MGI

brain development

Inferred from mutant phenotype PubMed 14566948PubMed 15295034. Source: MGI

cerebral cortex development

Inferred from mutant phenotype PubMed 19820705. Source: MGI

dorsal/ventral pattern formation

Inferred from mutant phenotype PubMed 11165475PubMed 14566948. Source: MGI

mesoderm development

Inferred from mutant phenotype PubMed 9247336. Source: MGI

negative regulation of transcription regulatory region DNA binding

Inferred from genetic interaction PubMed 9697309. Source: MGI

nervous system development

Inferred from mutant phenotype PubMed 9247336. Source: MGI

neural tube closure

Inferred from mutant phenotype PubMed 11719201. Source: MGI

neurogenesis

Inferred from direct assay PubMed 18184563. Source: MGI

neuron differentiation

Inferred from mutant phenotype PubMed 15173589. Source: MGI

olfactory bulb development

Inferred from mutant phenotype PubMed 19820705. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 10431247PubMed 19146846. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay Ref.3. Source: UniProtKB

retina development in camera-type eye

Inferred from genetic interaction PubMed 19146846. Source: MGI

telencephalon development

Inferred from mutant phenotype PubMed 11165475PubMed 11719201PubMed 17493606PubMed 19820705. Source: MGI

telencephalon regionalization

Inferred from mutant phenotype PubMed 11719201. Source: MGI

transcription from RNA polymerase II promoter

Inferred from physical interaction PubMed 17991461. Source: GOC

   Cellular_componentintracellular

Inferred from direct assay PubMed 10482234. Source: MGI

nucleus

Inferred from direct assay PubMed 18076286. Source: MGI

   Molecular_functionRNA polymerase II transcription factor binding transcription factor activity

Inferred from physical interaction PubMed 17991461. Source: MGI

chromatin binding

Inferred from direct assay PubMed 19146846. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 15173589. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 406406LIM/homeobox protein Lhx2
PRO_0000075779

Regions

Domain53 – 10553LIM zinc-binding 1
Domain115 – 16854LIM zinc-binding 2
DNA binding266 – 32560Homeobox
Motif307 – 32317Nuclear localization signal Potential
Compositional bias187 – 19610Poly-Ala

Experimental info

Mutagenesis531C → A: Reduces transcriptional activation; when associated with A-56. Ref.3
Mutagenesis561C → A: Reduces transcriptional activation; when associated with A-53. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9Z0S2 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: FE7B4E76454D6A90

FASTA40644,419
        10         20         30         40         50         60 
MLFHSLSGPE VHGVIDEMDR RAKSEAPAIS SAIDRGDTET TMPSISSDRA ALCAGCGGKI 

        70         80         90        100        110        120 
SDRYYLLAVD KQWHMRCLKC CECKLNLESE LTCFSKDGSI YCKEDYYRRF SVQRCARCHL 

       130        140        150        160        170        180 
GISASEMVMR ARDLVYHLNC FTCTTCNKML TTGDHFGMKD SLVYCRLHFE ALLQGEYPAH 

       190        200        210        220        230        240 
FNHADVAAAA AAAAAAKSAG LGSAGANPLG LPYYNGVGTV QKGRPRKRKS PGPGADLAAY 

       250        260        270        280        290        300 
NAALSCNEND AEHLDRDQPY PSSQKTKRMR TSFKHHQLRT MKSYFAINHN PDAKDLKQLA 

       310        320        330        340        350        360 
QKTGLTKRVL QVWFQNARAK FRRNLLRQEN TGVDKTSDAT LQTGTPSGPA SELSNASLSP 

       370        380        390        400 
SSTPTTLTDL TSPTLPTVTS VLTSVPGNLE GHEPHSPSQT TLTNLF 

« Hide

References

« Hide 'large scale' references
[1]"Conservation of the expression and function of apterous orthologs in Drosophila and mammals."
Rincon-Limas D.E., Lu C.-H., Canal I., Calleja M., Rodriguez-Esteban C., Izpisua-Belmonte J.-C., Botas J.
Proc. Natl. Acad. Sci. U.S.A. 96:2165-2170(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"MRG1 binds to the LIM domain of Lhx2 and may function as a coactivator to stimulate glycoprotein hormone alpha-subunit gene expression."
Glenn D.J., Maurer R.A.
J. Biol. Chem. 274:36159-36167(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CITED2, MUTAGENESIS OF CYS-53 AND CYS-56.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF124734 mRNA. Translation: AAD20012.1.
BC055741 mRNA. Translation: AAH55741.1.
RefSeqNP_034840.1. NM_010710.3.
UniGeneMm.142856.

3D structure databases

ProteinModelPortalQ9Z0S2.
SMRQ9Z0S2. Positions 5-174, 262-337.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201155. 6 interactions.

PTM databases

PhosphoSiteQ9Z0S2.

Proteomic databases

PaxDbQ9Z0S2.
PRIDEQ9Z0S2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000253; ENSMUSP00000000253; ENSMUSG00000000247.
GeneID16870.
KEGGmmu:16870.
UCSCuc008jnk.1. mouse.

Organism-specific databases

CTD9355.
MGIMGI:96785. Lhx2.

Phylogenomic databases

eggNOGNOG240987.
GeneTreeENSGT00750000117279.
HOGENOMHOG000034022.
HOVERGENHBG006262.
InParanoidQ9Z0S2.
KOK09373.
OMAHECRSPS.
PhylomeDBQ9Z0S2.
TreeFamTF315442.

Gene expression databases

ArrayExpressQ9Z0S2.
BgeeQ9Z0S2.
CleanExMM_LHX2.
GenevestigatorQ9Z0S2.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio290834.
PROQ9Z0S2.
SOURCESearch...

Entry information

Entry nameLHX2_MOUSE
AccessionPrimary (citable) accession number: Q9Z0S2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot