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Protein

3'(2'),5'-bisphosphate nucleotidase 1

Gene

Bpnt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Has 1000-fold lower activity towards inositol 1,4-bisphosphate (Ins(1,4)P2) and inositol 1,3,4-trisphosphate (Ins(1,3,4)P3), but does not hydrolyze Ins1P, Ins(3,4)P2, Ins(1,3,4,5)P4 or InsP6.

Catalytic activityi

Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.

Cofactori

Enzyme regulationi

Uncompetitive inhibition by micromolar concentrations of lithium. Competitive inhibition by inositol 1,4-bisphosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi74Magnesium 1By similarity1
Binding sitei74SubstrateBy similarity1
Metal bindingi117Magnesium 1By similarity1
Metal bindingi117Magnesium 2By similarity1
Metal bindingi119Magnesium 1; via carbonyl oxygenBy similarity1
Metal bindingi120Magnesium 2By similarity1
Metal bindingi247Magnesium 2By similarity1
Binding sitei247SubstrateBy similarity1

GO - Molecular functioni

  • 3'(2'),5'-bisphosphate nucleotidase activity Source: MGI
  • magnesium ion binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Lithium, Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.3.7. 3474.
ReactomeiR-MMU-156584. Cytosolic sulfonation of small molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
3'(2'),5'-bisphosphate nucleotidase 1 (EC:3.1.3.7)
Alternative name(s):
Bisphosphate 3'-nucleotidase 1
PAP-inositol 1,4-phosphatase
Short name:
PIP
Gene namesi
Name:Bpnt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1338800. Bpnt1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001425282 – 3083'(2'),5'-bisphosphate nucleotidase 1Add BLAST307

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei122PhosphothreonineBy similarity1
Modified residuei240PhosphoserineBy similarity1
Modified residuei244N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z0S1.
MaxQBiQ9Z0S1.
PaxDbiQ9Z0S1.
PeptideAtlasiQ9Z0S1.
PRIDEiQ9Z0S1.

2D gel databases

REPRODUCTION-2DPAGEQ9Z0S1.

PTM databases

iPTMnetiQ9Z0S1.
PhosphoSitePlusiQ9Z0S1.
SwissPalmiQ9Z0S1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026617.
CleanExiMM_BPNT1.
ExpressionAtlasiQ9Z0S1. baseline and differential.
GenevisibleiQ9Z0S1. MM.

Interactioni

Protein-protein interaction databases

BioGridi204743. 1 interactor.
IntActiQ9Z0S1. 3 interactors.
MINTiMINT-1854625.
STRINGi10090.ENSMUSP00000027916.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0S1.
SMRiQ9Z0S1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni119 – 122Substrate bindingBy similarity4
Regioni195 – 198Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the inositol monophosphatase family.Curated

Phylogenomic databases

eggNOGiKOG3099. Eukaryota.
ENOG410XNMV. LUCA.
GeneTreeiENSGT00530000063462.
HOGENOMiHOG000293205.
HOVERGENiHBG050719.
InParanoidiQ9Z0S1.
KOiK01082.
OMAiEEELVVW.
OrthoDBiEOG091G0CFP.
PhylomeDBiQ9Z0S1.
TreeFamiTF314300.

Family and domain databases

InterProiIPR020583. Inositol_monoP_metal-BS.
IPR000760. Inositol_monophosphatase-like.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PROSITEiPS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSHTVLMR LVASAYSIAQ KAGTIVRCVI AEGDLGIVQK TSATDLQTKA
60 70 80 90 100
DRLVQMSICS SLARKFPKLT IIGEEDLPPG EVDQELIEDG QWEEILKQPC
110 120 130 140 150
PSQYSAIKEE DLVVWVDPLD GTKEYTEGLL DNVTVLIGIA YEGKAIAGII
160 170 180 190 200
NQPYYNYQAG PDAALGRTIW GVLGLGAFGF QLKEAPAGKH IITTTRSHSN
210 220 230 240 250
QLVTDCISAM NPDTVLRVGG AGNKIIQLIE GKASAYVFAS PGCKKWDTCA
260 270 280 290 300
PEVILHAVGG KLTDIHGNAL QYNKEVKHMN SAGVLAALRN YEYYASHVPE

SVKNALIP
Length:308
Mass (Da):33,196
Last modified:April 13, 2004 - v2
Checksum:iD941FEA4C2E59E9D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164A → V in AAH11036 (PubMed:15489334).Curated1
Sequence conflicti190H → R in AAH11036 (PubMed:15489334).Curated1
Sequence conflicti276V → A in AAD17330 (PubMed:10224133).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125043 mRNA. Translation: AAD17330.1.
AK008714 mRNA. Translation: BAB25850.1.
AK151931 mRNA. Translation: BAE30807.1.
AK152009 mRNA. Translation: BAE30872.1.
BC011036 mRNA. Translation: AAH11036.1.
CCDSiCCDS15597.1.
RefSeqiNP_035924.2. NM_011794.3.
UniGeneiMm.227549.

Genome annotation databases

EnsembliENSMUST00000027916; ENSMUSP00000027916; ENSMUSG00000026617.
GeneIDi23827.
KEGGimmu:23827.
UCSCiuc007dzc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF125043 mRNA. Translation: AAD17330.1.
AK008714 mRNA. Translation: BAB25850.1.
AK151931 mRNA. Translation: BAE30807.1.
AK152009 mRNA. Translation: BAE30872.1.
BC011036 mRNA. Translation: AAH11036.1.
CCDSiCCDS15597.1.
RefSeqiNP_035924.2. NM_011794.3.
UniGeneiMm.227549.

3D structure databases

ProteinModelPortaliQ9Z0S1.
SMRiQ9Z0S1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204743. 1 interactor.
IntActiQ9Z0S1. 3 interactors.
MINTiMINT-1854625.
STRINGi10090.ENSMUSP00000027916.

PTM databases

iPTMnetiQ9Z0S1.
PhosphoSitePlusiQ9Z0S1.
SwissPalmiQ9Z0S1.

2D gel databases

REPRODUCTION-2DPAGEQ9Z0S1.

Proteomic databases

EPDiQ9Z0S1.
MaxQBiQ9Z0S1.
PaxDbiQ9Z0S1.
PeptideAtlasiQ9Z0S1.
PRIDEiQ9Z0S1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027916; ENSMUSP00000027916; ENSMUSG00000026617.
GeneIDi23827.
KEGGimmu:23827.
UCSCiuc007dzc.1. mouse.

Organism-specific databases

CTDi10380.
MGIiMGI:1338800. Bpnt1.

Phylogenomic databases

eggNOGiKOG3099. Eukaryota.
ENOG410XNMV. LUCA.
GeneTreeiENSGT00530000063462.
HOGENOMiHOG000293205.
HOVERGENiHBG050719.
InParanoidiQ9Z0S1.
KOiK01082.
OMAiEEELVVW.
OrthoDBiEOG091G0CFP.
PhylomeDBiQ9Z0S1.
TreeFamiTF314300.

Enzyme and pathway databases

BRENDAi3.1.3.7. 3474.
ReactomeiR-MMU-156584. Cytosolic sulfonation of small molecules.

Miscellaneous databases

PROiQ9Z0S1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026617.
CleanExiMM_BPNT1.
ExpressionAtlasiQ9Z0S1. baseline and differential.
GenevisibleiQ9Z0S1. MM.

Family and domain databases

InterProiIPR020583. Inositol_monoP_metal-BS.
IPR000760. Inositol_monophosphatase-like.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PROSITEiPS00629. IMP_1. 1 hit.
PS00630. IMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBPNT1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0S1
Secondary accession number(s): Q3U8Z5, Q91XB9, Q9D7Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: April 13, 2004
Last modified: November 30, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.