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Q9Z0R9 (FADS2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fatty acid desaturase 2

EC=1.14.19.-
Alternative name(s):
Delta(6) fatty acid desaturase
Short name=D6D
Short name=Delta(6) desaturase
Short name=Delta-6 desaturase
Gene names
Name:Fads2
Synonyms:Fadsd2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions By similarity.

Pathway

Lipid metabolism; polyunsaturated fatty acid biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.

Tissue specificity

Highly expressed in the adrenal gland, liver, brain, and testis, tissues where lipogenesis and steroidogenesis are active. Also detected in lung, heart, and skeletal muscle. Ref.1 Ref.4

Developmental stage

Found in 13-day-old embryo heart.

Induction

Induced by dietary PUFA-deficient diet. Induced by a fat-free diet and by a diet containing triolein (18:1n-9) as the only fat source. Down-regulated in liver by dietary PUFA. Ref.1 Ref.4

Domain

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similarities

Belongs to the fatty acid desaturase family.

Contains 1 cytochrome b5 heme-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Fatty acid desaturase 2
PRO_0000307103

Regions

Topological domain1 – 130130Cytoplasmic Potential
Transmembrane131 – 15121Helical; Potential
Topological domain1521Lumenal Potential
Transmembrane153 – 17321Helical; Potential
Topological domain174 – 26491Cytoplasmic Potential
Transmembrane265 – 28521Helical; Potential
Topological domain286 – 30520Lumenal Potential
Transmembrane306 – 32621Helical; Potential
Topological domain327 – 444118Cytoplasmic Potential
Domain18 – 9578Cytochrome b5 heme-binding
Motif180 – 1845Histidine box-1
Motif217 – 2215Histidine box-2
Motif382 – 3865Histidine box-3

Sequences

Sequence LengthMass (Da)Tools
Q9Z0R9 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 7615D17024D3B771

FASTA44452,387
        10         20         30         40         50         60 
MGKGGNQGEG STERQAPMPT FRWEEIQKHN LRTDRWLVID RKVYNVTKWS QRHPGGHRVI 

        70         80         90        100        110        120 
GHYSGEDATD AFRAFHLDLD FVGKFLKPLL IGELAPEEPS LDRGKSSQIT EDFRALKKTA 

       130        140        150        160        170        180 
EDMNLFKTNH LFFFLLLSHI IVMESLAWFI LSYFGTGWIP TLVTAFVLAT SQAQAGWLQH 

       190        200        210        220        230        240 
DYGHLSVYKK SIWNHVVHKF VIGHLKGASA NWWNHRHFQH HAKPNIFHKD PDIKSLHVFV 

       250        260        270        280        290        300 
LGEWQPLEYG KKKLKYLPYN HQHEYFFLIG PPLLIPMYFQ YQIIMTMISR RDWVDLAWAI 

       310        320        330        340        350        360 
SYYMRFFYTY IPFYGILGAL VFLNFIRFLE SHWFVWVTQM NHLVMEIDLD HYRDWFSSQL 

       370        380        390        400        410        420 
AATCNVEQSF FNDWFSGHLN FQIEHHLFPT MPRHNLHKIA PLVKSLCAKH GIEYQEKPLL 

       430        440 
RALIDIVSSL KKSGELWLDA YLHK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, and nutritional regulation of the mammalian Delta-6 desaturase."
Cho H.P., Nakamura M.T., Clarke S.D.
J. Biol. Chem. 274:471-477(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[4]"Dual regulation of mouse Delta(5)- and Delta(6)-desaturase gene expression by SREBP-1 and PPARalpha."
Matsuzaka T., Shimano H., Yahagi N., Amemiya-Kudo M., Yoshikawa T., Hasty A.H., Tamura Y., Osuga J., Okazaki H., Iizuka Y., Takahashi A., Sone H., Gotoda T., Ishibashi S., Yamada N.
J. Lipid Res. 43:107-114(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
Strain: C57BL/6J.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF126798 mRNA. Translation: AAD20017.1.
AK143524 mRNA. Translation: BAE25416.1.
BC057189 mRNA. Translation: AAH57189.1.
RefSeqNP_062673.1. NM_019699.1.
UniGeneMm.38901.

3D structure databases

ProteinModelPortalQ9Z0R9.
SMRQ9Z0R9. Positions 21-98.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9Z0R9. 1 interaction.
MINTMINT-4131280.
STRING10090.ENSMUSP00000025567.

Chemistry

BindingDBQ9Z0R9.
ChEMBLCHEMBL5087.

PTM databases

PhosphoSiteQ9Z0R9.

Proteomic databases

PaxDbQ9Z0R9.
PRIDEQ9Z0R9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025567; ENSMUSP00000025567; ENSMUSG00000024665.
GeneID56473.
KEGGmmu:56473.
UCSCuc008gpb.1. mouse.

Organism-specific databases

CTD9415.
MGIMGI:1930079. Fads2.

Phylogenomic databases

eggNOGNOG70688.
GeneTreeENSGT00510000046574.
HOGENOMHOG000012997.
HOVERGENHBG002839.
InParanoidQ9Z0R9.
KOK10226.
OMAQPLEYGK.
OrthoDBEOG7G1V6P.
PhylomeDBQ9Z0R9.
TreeFamTF313604.

Enzyme and pathway databases

UniPathwayUPA00658.

Gene expression databases

ArrayExpressQ9Z0R9.
BgeeQ9Z0R9.
CleanExMM_FADS2.
GenevestigatorQ9Z0R9.

Family and domain databases

Gene3D3.10.120.10. 1 hit.
InterProIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR012171. Fatty_acid/sphinglp_desaturase.
IPR005804. Fatty_acid_desaturase-1.
[Graphical view]
PfamPF00173. Cyt-b5. 1 hit.
PF00487. FA_desaturase. 1 hit.
[Graphical view]
PIRSFPIRSF015921. FA_sphinglp_des. 1 hit.
SUPFAMSSF55856. SSF55856. 1 hit.
PROSITEPS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFADS2. mouse.
NextBio312742.
PROQ9Z0R9.
SOURCESearch...

Entry information

Entry nameFADS2_MOUSE
AccessionPrimary (citable) accession number: Q9Z0R9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot