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Protein

Intersectin-2

Gene

Itsn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). Plays a role in dendrite formation by melanocytes.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi67 – 7913PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Differentiation, Endocytosis

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Intersectin-2
Alternative name(s):
EH domain and SH3 domain regulator of endocytosis 2
Short name:
EH and SH3 domains protein 2
SH3 domain-containing protein 1B
Gene namesi
Name:Itsn2
Synonyms:Ese2, Sh3d1B
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1338049. Itsn2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16591659Intersectin-2PRO_0000080962Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei110 – 1101PhosphoserineBy similarity
Modified residuei211 – 2111PhosphoserineCombined sources
Modified residuei231 – 2311PhosphoserineBy similarity
Modified residuei554 – 5541PhosphotyrosineCombined sources
Modified residuei574 – 5741PhosphothreonineBy similarity
Modified residuei836 – 8361PhosphothreonineCombined sources
Modified residuei838 – 8381PhosphoserineCombined sources
Modified residuei843 – 8431PhosphoserineCombined sources
Modified residuei922 – 9221PhosphotyrosineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z0R6.
MaxQBiQ9Z0R6.
PaxDbiQ9Z0R6.
PeptideAtlasiQ9Z0R6.
PRIDEiQ9Z0R6.

PTM databases

iPTMnetiQ9Z0R6.
PhosphoSiteiQ9Z0R6.

Expressioni

Tissue specificityi

Widely expressed in adult tissues.

Developmental stagei

Widely distributed throughout the adult forebrain. Prominent expression was observed in the neocortex, the piriform cortex, the pyramidal cell layers of hippocampus, the dentate gyrus, in several nuclei of the thalamus and hypothalamus and in the amygdala.

Gene expression databases

CleanExiMM_ITSN2.

Interactioni

Subunit structurei

Belongs to a complex that may contain multimers of ITSN1, ITSN2 and EPS15, and different partners according to the step in the endocytic process. Interacts with ADAM15. Interacts with FASLG (By similarity). Interacts with ANKRD54. Interacts with FCHO2.By similarity2 Publications

Protein-protein interaction databases

IntActiQ9Z0R6. 12 interactions.
MINTiMINT-4099185.
STRINGi10090.ENSMUSP00000052758.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0R6.
SMRiQ9Z0R6. Positions 2-108, 237-333, 717-804, 855-909, 945-999, 1010-1516, 1530-1653.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 11089EH 1PROSITE-ProRule annotationAdd
BLAST
Domaini54 – 8936EF-handPROSITE-ProRule annotationAdd
BLAST
Domaini245 – 33490EH 2PROSITE-ProRule annotationAdd
BLAST
Domaini718 – 77962SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini852 – 91059SH3 2PROSITE-ProRule annotationAdd
BLAST
Domaini942 – 100059SH3 3PROSITE-ProRule annotationAdd
BLAST
Domaini1014 – 107865SH3 4PROSITE-ProRule annotationAdd
BLAST
Domaini1088 – 114760SH3 5PROSITE-ProRule annotationAdd
BLAST
Domaini1170 – 1357188DHPROSITE-ProRule annotationAdd
BLAST
Domaini1396 – 1506111PHPROSITE-ProRule annotationAdd
BLAST
Domaini1518 – 161497C2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili365 – 717353Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 2 EH domains.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 5 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG1029. Eukaryota.
KOG4305. Eukaryota.
COG5422. LUCA.
HOGENOMiHOG000010175.
HOVERGENiHBG052159.
InParanoidiQ9Z0R6.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR000219. DH-domain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR027029. Intersectin-2.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR11216:SF29. PTHR11216:SF29. 5 hits.
PfamiPF00168. C2. 1 hit.
PF12763. EF-hand_4. 2 hits.
PF16652. PH_13. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 2 hits.
PF07653. SH3_2. 1 hit.
PF14604. SH3_9. 2 hits.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00027. EH. 2 hits.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 5 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
SSF48065. SSF48065. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50044. SSF50044. 5 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50010. DH_2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS50031. EH. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z0R6-1) [UniParc]FASTAAdd to basket
Also known as: Ese2L, Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMAQFPTAMN GGPNMWAITS EERTKHDKQF DNLKPSGGYI TGDQARTFFL
60 70 80 90 100
QSGLPAPVLA EIWALSDLNK DGKMDQQEFS IAMKLIKLKL QGQQLPVVLP
110 120 130 140 150
PIMKQPPMFS PLISARFGMG SMPNLSIHQP LPPVAPIATP LSSATSGTSI
160 170 180 190 200
PPLMMPAPLV PSVSTSSLPN GTASLIQPLS IPYSSSTLPH ASSYSLMMGG
210 220 230 240 250
FGGASIQKAQ SLIDLGSSSS TSSTASLSGN SPKTGTSEWA VPQPSRLKYR
260 270 280 290 300
QKFNSLDKGM SGYLSGFQAR NALLQSNLSQ TQLATIWTLA DIDGDGQLKA
310 320 330 340 350
EEFILAMHLT DMAKAGQPLP LTLPPELVPP SFRGGKQVDS VNGTLPSYQK
360 370 380 390 400
TQEEEPQKKL PVTFEDKRKA NYERGNMELE KRRQVLMEQQ QREAERKAQK
410 420 430 440 450
EKEEWERKQR ELQEQEWKKQ LELEKRLEKQ RELERQREEE RRKEIERREA
460 470 480 490 500
AKQELERQRR LEWERLRRQE LLSQKTREQE DIVRLSSRKK SLHLELEAVN
510 520 530 540 550
GKHQQISGRL QDVQIRKQTQ KTELEVLDKQ CDLEIMEIKQ LQQELKEYQN
560 570 580 590 600
KLIYLVPEKQ LLNERIKNMQ LSNTPDSGIS LLHKKSSEKE ELCQRLKEQL
610 620 630 640 650
DALEKETASK LSEMDSFNNQ LKELRESYNT QQLALEQLHK IKRDKLKEIE
660 670 680 690 700
RKRLEQIQKK KLEDEAARKA KQGKENLWRE SIRKEEEEKQ KRLQEEKSQD
710 720 730 740 750
KTQEEERKAE AKQSETASAL VNYRALYPFE ARNHDEMSFS SGDIIQVDEK
760 770 780 790 800
TVGEPGWLYG SFQGKFGWFP CNYVEKVLSS EKALSPKKAL LPPTVSLSAT
810 820 830 840 850
STSSQPPASV TDYHNVSFSN LTVNTTWQQK SAFTRTVSPG SVSPIHGQGQ
860 870 880 890 900
AVENLKAQAL CSWTAKKENH LNFSKHDVIT VLEQQENWWF GEVHGGRGWF
910 920 930 940 950
PKSYVKLIPG NEVQRGEPEA LYAAVTKKPT STAYPVTSTA YPVGEDYIAL
960 970 980 990 1000
YSYSSVEPGD LTFTEGEEIL VTQKDGEWWT GSIGERTGIF PSNYVRPKDQ
1010 1020 1030 1040 1050
ENFGNASKSG ASNKKPEIAQ VTSAYAASGT EQLSLAPGQL ILILKKNTSG
1060 1070 1080 1090 1100
WWQGELQARG KKRQKGWFPA SHVKLLGPSS ERTMPTFHAV CQVIAMYDYM
1110 1120 1130 1140 1150
ANNEDELNFS KGQLINVMNK DDPDWWQGET NGLTGLFPSN YVKMTTDSDP
1160 1170 1180 1190 1200
SQQWCADLQA LDTMQPTERK RQGYIHELIQ TEERYMDDDL QLVIEVFQKR
1210 1220 1230 1240 1250
MAEEGFLTEA DMALIFVNWK ELIMSNTKLL RALRVRKKTG GEKMPVQMIG
1260 1270 1280 1290 1300
DILAAELSHM QAYIRFCSCQ LNGATLLQQK TDEDTDFKEF LKKLASDPRC
1310 1320 1330 1340 1350
KGMPLSSFLL KPMQRITRYP LLIRSILENT PQSHVDHSSL KLALERAEEL
1360 1370 1380 1390 1400
CSQVNEGVRE KENSDRLEWI QAHVQCEGLA EQLIFNSLTN CLGPRKLLHS
1410 1420 1430 1440 1450
GKLYKTKSNK ELHAFLFNDF LLLTYLVRQF AAASGHEKLF NSKSSAQFRM
1460 1470 1480 1490 1500
YKTPIFLNEV LVKLPTDPSG DEPVFHISHI DRVYTLRTDN INERTAWVQK
1510 1520 1530 1540 1550
IKGASEQYID TEKKKREKAY QARSQKTSGI GRLMVHVIEA TELKACKPNG
1560 1570 1580 1590 1600
KSNPYCEVSM GSQSYTTRTL QDTLNPKWNF NCQFFIKDLY QDVLCLTMFD
1610 1620 1630 1640 1650
RDQFSPDDFL GRTEVPVAKI RTEQESKGPT TRRLLLHEVP TGEVWVRFDL

QLFEQKTLL
Length:1,659
Mass (Da):188,908
Last modified:April 20, 2010 - v2
Checksum:i840F16B3A0DACDD6
GO
Isoform 2 (identifier: Q9Z0R6-2) [UniParc]FASTAAdd to basket
Also known as: Ese2, Short

The sequence of this isoform differs from the canonical sequence as follows:
     1188-1198: DDLQLVIEVFQ → GLQLFEQKTLL
     1199-1659: Missing.

Show »
Length:1,198
Mass (Da):135,782
Checksum:iE06BDE54E64D1425
GO

Sequence cautioni

The sequence BAC31264 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti452 – 4532KQ → NT in AAD19748 (PubMed:10064583).Curated
Sequence conflicti1162 – 11621D → G in AAD19748 (PubMed:10064583).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1188 – 119811DDLQLVIEVFQ → GLQLFEQKTLL in isoform 2. 1 PublicationVSP_003896Add
BLAST
Alternative sequencei1199 – 1659461Missing in isoform 2. 1 PublicationVSP_003897Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042449 mRNA. Translation: BAC31264.1. Different initiation.
AF132479 mRNA. Translation: AAD19747.1.
AF132480 mRNA. Translation: AAD19748.1.
UniGeneiMm.341204.

Genome annotation databases

UCSCiuc007mxy.1. mouse. [Q9Z0R6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042449 mRNA. Translation: BAC31264.1. Different initiation.
AF132479 mRNA. Translation: AAD19747.1.
AF132480 mRNA. Translation: AAD19748.1.
UniGeneiMm.341204.

3D structure databases

ProteinModelPortaliQ9Z0R6.
SMRiQ9Z0R6. Positions 2-108, 237-333, 717-804, 855-909, 945-999, 1010-1516, 1530-1653.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z0R6. 12 interactions.
MINTiMINT-4099185.
STRINGi10090.ENSMUSP00000052758.

PTM databases

iPTMnetiQ9Z0R6.
PhosphoSiteiQ9Z0R6.

Proteomic databases

EPDiQ9Z0R6.
MaxQBiQ9Z0R6.
PaxDbiQ9Z0R6.
PeptideAtlasiQ9Z0R6.
PRIDEiQ9Z0R6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc007mxy.1. mouse. [Q9Z0R6-2]

Organism-specific databases

MGIiMGI:1338049. Itsn2.

Phylogenomic databases

eggNOGiKOG1029. Eukaryota.
KOG4305. Eukaryota.
COG5422. LUCA.
HOGENOMiHOG000010175.
HOVERGENiHBG052159.
InParanoidiQ9Z0R6.

Miscellaneous databases

ChiTaRSiItsn2. mouse.
PROiQ9Z0R6.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ITSN2.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR000219. DH-domain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR027029. Intersectin-2.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR11216:SF29. PTHR11216:SF29. 5 hits.
PfamiPF00168. C2. 1 hit.
PF12763. EF-hand_4. 2 hits.
PF16652. PH_13. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 2 hits.
PF07653. SH3_2. 1 hit.
PF14604. SH3_9. 2 hits.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00239. C2. 1 hit.
SM00054. EFh. 2 hits.
SM00027. EH. 2 hits.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 5 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
SSF48065. SSF48065. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50044. SSF50044. 5 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50010. DH_2. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS50031. EH. 2 hits.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITSN2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0R6
Secondary accession number(s): Q8C9C3, Q9Z0R5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: April 20, 2010
Last modified: July 6, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.