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Protein

Intersectin-2

Gene

Itsn2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). Plays a role in dendrite formation by melanocytes.By similarity

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi67 – 79PROSITE-ProRule annotationAdd BLAST13

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDifferentiation, Endocytosis
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Intersectin-2
Alternative name(s):
EH domain and SH3 domain regulator of endocytosis 2
Short name:
EH and SH3 domains protein 2
SH3 domain-containing protein 1B
Gene namesi
Name:Itsn2
Synonyms:Ese2, Sh3d1B
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:1338049 Itsn2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000809621 – 1659Intersectin-2Add BLAST1659

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineBy similarity1
Modified residuei211PhosphoserineCombined sources1
Modified residuei231PhosphoserineBy similarity1
Modified residuei554PhosphotyrosineCombined sources1
Modified residuei574PhosphothreonineBy similarity1
Modified residuei836PhosphothreonineCombined sources1
Modified residuei838PhosphoserineCombined sources1
Modified residuei843PhosphoserineCombined sources1
Modified residuei922PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9Z0R6
PaxDbiQ9Z0R6
PeptideAtlasiQ9Z0R6
PRIDEiQ9Z0R6

PTM databases

iPTMnetiQ9Z0R6
PhosphoSitePlusiQ9Z0R6

Expressioni

Tissue specificityi

Widely expressed in adult tissues.

Developmental stagei

Widely distributed throughout the adult forebrain. Prominent expression was observed in the neocortex, the piriform cortex, the pyramidal cell layers of hippocampus, the dentate gyrus, in several nuclei of the thalamus and hypothalamus and in the amygdala.

Gene expression databases

CleanExiMM_ITSN2

Interactioni

Subunit structurei

Belongs to a complex that may contain multimers of ITSN1, ITSN2 and EPS15, and different partners according to the step in the endocytic process. Interacts with ADAM15. Interacts with FASLG (By similarity). Interacts with ANKRD54. Interacts with FCHO2.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9Z0R6, 14 interactors
MINTiQ9Z0R6
STRINGi10090.ENSMUSP00000052758

Structurei

3D structure databases

ProteinModelPortaliQ9Z0R6
SMRiQ9Z0R6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 110EH 1PROSITE-ProRule annotationAdd BLAST89
Domaini54 – 89EF-handPROSITE-ProRule annotationAdd BLAST36
Domaini245 – 334EH 2PROSITE-ProRule annotationAdd BLAST90
Domaini718 – 779SH3 1PROSITE-ProRule annotationAdd BLAST62
Domaini852 – 910SH3 2PROSITE-ProRule annotationAdd BLAST59
Domaini942 – 1000SH3 3PROSITE-ProRule annotationAdd BLAST59
Domaini1014 – 1078SH3 4PROSITE-ProRule annotationAdd BLAST65
Domaini1088 – 1147SH3 5PROSITE-ProRule annotationAdd BLAST60
Domaini1170 – 1357DHPROSITE-ProRule annotationAdd BLAST188
Domaini1396 – 1506PHPROSITE-ProRule annotationAdd BLAST111
Domaini1518 – 1614C2PROSITE-ProRule annotationAdd BLAST97

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili365 – 717Sequence analysisAdd BLAST353

Keywords - Domaini

Coiled coil, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG1029 Eukaryota
KOG4305 Eukaryota
COG5422 LUCA
HOGENOMiHOG000010175
HOVERGENiHBG052159
InParanoidiQ9Z0R6

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
cd11988 SH3_Intersectin2_1, 1 hit
cd11990 SH3_Intersectin2_2, 1 hit
cd11992 SH3_Intersectin2_3, 1 hit
cd11994 SH3_Intersectin2_4, 1 hit
cd11996 SH3_Intersectin2_5, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR000261 EH_dom
IPR027029 Intersectin-2
IPR035737 Intersectin-2_SH3_1
IPR035738 Intersectin-2_SH3_2
IPR035739 Intersectin-2_SH3_3
IPR035740 Intersectin-2_SH3_4
IPR035741 Intersectin-2_SH3_5
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR11216:SF29 PTHR11216:SF29, 4 hits
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF12763 EF-hand_4, 2 hits
PF16652 PH_13, 1 hit
PF00621 RhoGEF, 1 hit
PF00018 SH3_1, 2 hits
PF07653 SH3_2, 1 hit
PF14604 SH3_9, 2 hits
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00054 EFh, 2 hits
SM00027 EH, 2 hits
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00326 SH3, 5 hits
SUPFAMiSSF47473 SSF47473, 2 hits
SSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 5 hits
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50010 DH_2, 1 hit
PS00018 EF_HAND_1, 1 hit
PS50222 EF_HAND_2, 2 hits
PS50031 EH, 2 hits
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 5 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z0R6-1) [UniParc]FASTAAdd to basket
Also known as: Ese2L, Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMAQFPTAMN GGPNMWAITS EERTKHDKQF DNLKPSGGYI TGDQARTFFL
60 70 80 90 100
QSGLPAPVLA EIWALSDLNK DGKMDQQEFS IAMKLIKLKL QGQQLPVVLP
110 120 130 140 150
PIMKQPPMFS PLISARFGMG SMPNLSIHQP LPPVAPIATP LSSATSGTSI
160 170 180 190 200
PPLMMPAPLV PSVSTSSLPN GTASLIQPLS IPYSSSTLPH ASSYSLMMGG
210 220 230 240 250
FGGASIQKAQ SLIDLGSSSS TSSTASLSGN SPKTGTSEWA VPQPSRLKYR
260 270 280 290 300
QKFNSLDKGM SGYLSGFQAR NALLQSNLSQ TQLATIWTLA DIDGDGQLKA
310 320 330 340 350
EEFILAMHLT DMAKAGQPLP LTLPPELVPP SFRGGKQVDS VNGTLPSYQK
360 370 380 390 400
TQEEEPQKKL PVTFEDKRKA NYERGNMELE KRRQVLMEQQ QREAERKAQK
410 420 430 440 450
EKEEWERKQR ELQEQEWKKQ LELEKRLEKQ RELERQREEE RRKEIERREA
460 470 480 490 500
AKQELERQRR LEWERLRRQE LLSQKTREQE DIVRLSSRKK SLHLELEAVN
510 520 530 540 550
GKHQQISGRL QDVQIRKQTQ KTELEVLDKQ CDLEIMEIKQ LQQELKEYQN
560 570 580 590 600
KLIYLVPEKQ LLNERIKNMQ LSNTPDSGIS LLHKKSSEKE ELCQRLKEQL
610 620 630 640 650
DALEKETASK LSEMDSFNNQ LKELRESYNT QQLALEQLHK IKRDKLKEIE
660 670 680 690 700
RKRLEQIQKK KLEDEAARKA KQGKENLWRE SIRKEEEEKQ KRLQEEKSQD
710 720 730 740 750
KTQEEERKAE AKQSETASAL VNYRALYPFE ARNHDEMSFS SGDIIQVDEK
760 770 780 790 800
TVGEPGWLYG SFQGKFGWFP CNYVEKVLSS EKALSPKKAL LPPTVSLSAT
810 820 830 840 850
STSSQPPASV TDYHNVSFSN LTVNTTWQQK SAFTRTVSPG SVSPIHGQGQ
860 870 880 890 900
AVENLKAQAL CSWTAKKENH LNFSKHDVIT VLEQQENWWF GEVHGGRGWF
910 920 930 940 950
PKSYVKLIPG NEVQRGEPEA LYAAVTKKPT STAYPVTSTA YPVGEDYIAL
960 970 980 990 1000
YSYSSVEPGD LTFTEGEEIL VTQKDGEWWT GSIGERTGIF PSNYVRPKDQ
1010 1020 1030 1040 1050
ENFGNASKSG ASNKKPEIAQ VTSAYAASGT EQLSLAPGQL ILILKKNTSG
1060 1070 1080 1090 1100
WWQGELQARG KKRQKGWFPA SHVKLLGPSS ERTMPTFHAV CQVIAMYDYM
1110 1120 1130 1140 1150
ANNEDELNFS KGQLINVMNK DDPDWWQGET NGLTGLFPSN YVKMTTDSDP
1160 1170 1180 1190 1200
SQQWCADLQA LDTMQPTERK RQGYIHELIQ TEERYMDDDL QLVIEVFQKR
1210 1220 1230 1240 1250
MAEEGFLTEA DMALIFVNWK ELIMSNTKLL RALRVRKKTG GEKMPVQMIG
1260 1270 1280 1290 1300
DILAAELSHM QAYIRFCSCQ LNGATLLQQK TDEDTDFKEF LKKLASDPRC
1310 1320 1330 1340 1350
KGMPLSSFLL KPMQRITRYP LLIRSILENT PQSHVDHSSL KLALERAEEL
1360 1370 1380 1390 1400
CSQVNEGVRE KENSDRLEWI QAHVQCEGLA EQLIFNSLTN CLGPRKLLHS
1410 1420 1430 1440 1450
GKLYKTKSNK ELHAFLFNDF LLLTYLVRQF AAASGHEKLF NSKSSAQFRM
1460 1470 1480 1490 1500
YKTPIFLNEV LVKLPTDPSG DEPVFHISHI DRVYTLRTDN INERTAWVQK
1510 1520 1530 1540 1550
IKGASEQYID TEKKKREKAY QARSQKTSGI GRLMVHVIEA TELKACKPNG
1560 1570 1580 1590 1600
KSNPYCEVSM GSQSYTTRTL QDTLNPKWNF NCQFFIKDLY QDVLCLTMFD
1610 1620 1630 1640 1650
RDQFSPDDFL GRTEVPVAKI RTEQESKGPT TRRLLLHEVP TGEVWVRFDL

QLFEQKTLL
Length:1,659
Mass (Da):188,908
Last modified:April 20, 2010 - v2
Checksum:i840F16B3A0DACDD6
GO
Isoform 2 (identifier: Q9Z0R6-2) [UniParc]FASTAAdd to basket
Also known as: Ese2, Short

The sequence of this isoform differs from the canonical sequence as follows:
     1188-1198: DDLQLVIEVFQ → GLQLFEQKTLL
     1199-1659: Missing.

Show »
Length:1,198
Mass (Da):135,782
Checksum:iE06BDE54E64D1425
GO

Sequence cautioni

The sequence BAC31264 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti452 – 453KQ → NT in AAD19748 (PubMed:10064583).Curated2
Sequence conflicti1162D → G in AAD19748 (PubMed:10064583).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0038961188 – 1198DDLQLVIEVFQ → GLQLFEQKTLL in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_0038971199 – 1659Missing in isoform 2. 1 PublicationAdd BLAST461

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042449 mRNA Translation: BAC31264.1 Different initiation.
AF132479 mRNA Translation: AAD19747.1
AF132480 mRNA Translation: AAD19748.1
UniGeneiMm.341204

Genome annotation databases

UCSCiuc007mxy.1 mouse [Q9Z0R6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiITSN2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0R6
Secondary accession number(s): Q8C9C3, Q9Z0R5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: April 20, 2010
Last modified: March 28, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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