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Q9Z0R0

- HASP_MOUSE

UniProt

Q9Z0R0 - HASP_MOUSE

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Protein
Serine/threonine-protein kinase haspin
Gene
Gsg2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that phosphorylates histone H3 at 'Ser-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Magnesium.1 Publication

Enzyme regulationi

Constitutive activity that does not require phosphorylation. Specifically inhibited by 3-(1H-indazol-5-yl)-N-propylimidazo[1,2-b]pyridazin-6-amine (CHR-6494) By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271Proton acceptor By similarityBy similarity
Binding sitei467 – 4671ATP By similarityBy similarity
Active sitei605 – 6051Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi446 – 4549ATP By similarityBy similarity
Nucleotide bindingi562 – 5676ATP By similarity
Nucleotide bindingi605 – 6106ATP By similarity
Nucleotide bindingi643 – 6453ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. DNA binding Source: MGI
  3. histone kinase activity (H3-T3 specific) Source: UniProtKB
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. histone H3-T3 phosphorylation involved in chromosome passenger complex localization to kinetochore Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. mitotic sister chromatid cohesion Source: UniProtKB
  4. negative regulation of G1/S transition of mitotic cell cycle Source: MGI
  5. protein localization to chromosome, centromeric region Source: UniProtKB
  6. protein phosphorylation Source: UniProtKB
  7. regulation of spindle checkpoint Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase haspin (EC:2.7.11.1)
Alternative name(s):
Germ cell-specific gene 2 protein
Haploid germ cell-specific nuclear protein kinase
Gene namesi
Name:Gsg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1194498. Gsg2.

Subcellular locationi

Nucleus. Chromosome By similarity. Cytoplasmcytoskeletonspindle By similarity
Note: Nuclear during interphase and associates with the chromosomes and spindle apparatus during mitosis By similarity.1 Publication

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. chromosome Source: UniProtKB-SubCell
  3. cytoplasm Source: UniProtKB-KW
  4. nuclear membrane Source: Ensembl
  5. nucleus Source: UniProtKB
  6. spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Serine/threonine-protein kinase haspin
PRO_0000085990Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551Phosphoserine By similarity

Post-translational modificationi

Autophosphorylated on both serine and threonine residues By similarity. Strongly phosphorylated during mitosis but this does not appear to significantly affect its intrinsic kinase activity. Phosphorylation by AURKB is required for full activity toward histone H3 at 'Ser-3' in mitosis By similarity.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9Z0R0.

PTM databases

PhosphoSiteiQ9Z0R0.

Expressioni

Tissue specificityi

Expressed in germ cells within the testis of adults and of embryos from day 24 onwards. Also present in adult thymus and weakly expressed in spleen, lung and whole embryo.3 Publications

Gene expression databases

ArrayExpressiQ9Z0R0.
BgeeiQ9Z0R0.
CleanExiMM_GSG2.
GenevestigatoriQ9Z0R0.

Interactioni

Protein-protein interaction databases

IntActiQ9Z0R0. 1 interaction.
STRINGi10090.ENSMUSP00000055806.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0R0.
SMRiQ9Z0R0. Positions 427-754.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini440 – 754315Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5072.
GeneTreeiENSGT00390000013015.
HOGENOMiHOG000168256.
HOVERGENiHBG049009.
InParanoidiQ9Z0R0.
KOiK16315.
OrthoDBiEOG744T89.
PhylomeDBiQ9Z0R0.
TreeFamiTF313895.

Family and domain databases

InterProiIPR024604. DUF3635.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12330. DUF3635. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z0R0-1 [UniParc]FASTAAdd to Basket

« Hide

MAQAHPRSGT RLFRTYAARG VRGSQRQPGG LAEQWFQPPN LKRAFSSSLS    50
DSNESPAVAS DDPDDPDFPG SLVGQRRRRP RGSGSRNQRT LTNTPRVQRL 100
RPRPPQKCST PCSRLRPPPF PNCSPGCLGS DHSVCIQSRD SNELGTSASL 150
FSSPASPGAP DPLYADSAVP GSFHLPAASL SEPSVPCPQV AATGDRYTGR 200
ALRAEASFRS SLFSLVNSGA TEENKFGTDG ENVKESCCER RQQMGNRLTD 250
PDLTSPGKRK AACKKVVSQG VDQRDYEESS ACKDLRVPGE ISRPKRTGPL 300
RKRKQQEATG TPPRHYHQSK KKRKASVSLW NLNTSQRDSW TKTRASFGFH 350
KKKIITSVIE VCSSVASSSS RSLLSECSTP PIKNRAHLTV SSRCSSVYLL 400
SPLKTLHVTD QRPSYAEKVY GECNQEGPIP FSDCLSTEKL ERCEKIGEGV 450
FGEVFQIIND QAPVALKIIA IEGLDLVNGS HQKTFEEILP EIIISKELSL 500
LSSEAYNRTE GFIGLNSVHC VQGLYPPLLL KAWDHYNTTK RSANDRPDFF 550
QEDQLFIILE FEFGGVDLER MKTKLSSVAT AKSILHQITA SLAVAEASLH 600
FEHRDLHWGN VLLKKTNLKE LRYTLNGKTS TIPTHGLQVN IIDYTLSRLE 650
RDGIVVFCDI SAEEDLFTGE GDYQFEIYRL MRKENKNCWG EYHPYNNVLW 700
LHYLTDKILN KMKFKTKCQS AAMKQIRKNL QHFHRTVLSF SSATDLLCQH 750
SLFR 754
Length:754
Mass (Da):84,181
Last modified:March 29, 2005 - v3
Checksum:i4D093D7C95E65308
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041P → L in BAA75494. 1 Publication
Sequence conflicti104 – 1041P → L in CAI24789. 1 Publication
Sequence conflicti305 – 3051Q → R in BAB00640. 1 Publication
Sequence conflicti305 – 3051Q → R in AAK30301. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87326 mRNA. Translation: BAA75494.1.
AB036930 Genomic DNA. Translation: BAB00640.1.
AF289866 Genomic DNA. Translation: AAK30301.1.
AL670399 Genomic DNA. Translation: CAI24789.1.
CCDSiCCDS24997.1.
RefSeqiNP_034483.1. NM_010353.2.
UniGeneiMm.42045.

Genome annotation databases

EnsembliENSMUST00000052140; ENSMUSP00000055806; ENSMUSG00000050107.
GeneIDi14841.
KEGGimmu:14841.
UCSCiuc007jzz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87326 mRNA. Translation: BAA75494.1 .
AB036930 Genomic DNA. Translation: BAB00640.1 .
AF289866 Genomic DNA. Translation: AAK30301.1 .
AL670399 Genomic DNA. Translation: CAI24789.1 .
CCDSi CCDS24997.1.
RefSeqi NP_034483.1. NM_010353.2.
UniGenei Mm.42045.

3D structure databases

ProteinModelPortali Q9Z0R0.
SMRi Q9Z0R0. Positions 427-754.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9Z0R0. 1 interaction.
STRINGi 10090.ENSMUSP00000055806.

PTM databases

PhosphoSitei Q9Z0R0.

Proteomic databases

PRIDEi Q9Z0R0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000052140 ; ENSMUSP00000055806 ; ENSMUSG00000050107 .
GeneIDi 14841.
KEGGi mmu:14841.
UCSCi uc007jzz.2. mouse.

Organism-specific databases

CTDi 83903.
MGIi MGI:1194498. Gsg2.

Phylogenomic databases

eggNOGi COG5072.
GeneTreei ENSGT00390000013015.
HOGENOMi HOG000168256.
HOVERGENi HBG049009.
InParanoidi Q9Z0R0.
KOi K16315.
OrthoDBi EOG744T89.
PhylomeDBi Q9Z0R0.
TreeFami TF313895.

Miscellaneous databases

NextBioi 287063.
PROi Q9Z0R0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Z0R0.
Bgeei Q9Z0R0.
CleanExi MM_GSG2.
Genevestigatori Q9Z0R0.

Family and domain databases

InterProi IPR024604. DUF3635.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view ]
Pfami PF12330. DUF3635. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of cDNA clones specifically expressed in testicular germ cells."
    Tanaka H., Yoshimura Y., Nishina Y., Nozaki M., Nojima H., Nishimune Y.
    FEBS Lett. 355:4-10(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Testis.
  2. "Identification and characterization of a haploid germ cell-specific nuclear protein kinase (Haspin) in spermatid nuclei and its effects on somatic cells."
    Tanaka H., Yoshimura Y., Nozaki M., Yomogida K., Tsuchida J., Tosaka Y., Habu T., Nakanishi T., Okada M., Nojima H., Nishimune Y.
    J. Biol. Chem. 274:17049-17057(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: C57BL/6.
    Tissue: Testis.
  3. "Nested genomic structure of haploid germ cell specific haspin gene."
    Yoshimura Y., Tanaka H., Nozaki M., Yomogida K., Yasunaga T., Nishimune Y.
    Gene 267:49-54(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The Haspin gene: location in an intron of the Integrin Alpha-E gene, associated transcription of an Integrin alpha-E-derived RNA and expression in diploid as well as haploid cells."
    Higgins J.M.G.
    Gene 267:55-69(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/Sv.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. "The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment."
    Dai J., Sultan S., Taylor S.S., Higgins J.M.G.
    Genes Dev. 19:472-488(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiHASP_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0R0
Secondary accession number(s): Q99MU9, Q9JJJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 29, 2005
Last modified: July 9, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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