Serine/threonine-protein kinase haspin

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Q9Z0R0 (HASP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 99. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase haspin
EC=2.7.11.1

Alternative name(s):
Germ cell-specific gene 2 protein
Haploid germ cell-specific nuclear protein kinase

Gene names

Name:

Gsg2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine-protein kinase that phosphorylates histone H3 at 'Ser-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle. Ref.2 Ref.6
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.2
Cofactor	Magnesium. Ref.2
Enzyme regulation	Constitutive activity that does not require phosphorylation. Specifically inhibited by 3-(1H-indazol-5-yl)-N-propylimidazo[1,2-b]pyridazin-6-amine (CHR-6494) By similarity.
Subcellular location	Nucleus. Chromosome By similarity. Cytoplasm › cytoskeleton › spindle By similarity. Note: Nuclear during interphase and associates with the chromosomes and spindle apparatus during mitosis By similarity. Ref.2
Tissue specificity	Expressed in germ cells within the testis of adults and of embryos from day 24 onwards. Also present in adult thymus and weakly expressed in spleen, lung and whole embryo. Ref.1 Ref.2 Ref.4
Post-translational modification	Autophosphorylated on both serine and threonine residues By similarity. Strongly phosphorylated during mitosis but this does not appear to significantly affect its intrinsic kinase activity. Phosphorylation by AURKB is required for full activity toward histone H3 at 'Ser-3' in mitosis By similarity. Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Chromosome Cytoplasm Cytoskeleton Nucleus
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Chromatin regulator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone H3-T3 phosphorylation involved in chromosome passenger complex localization to kinetochore Inferred from sequence or structural similarity. Source: UniProtKB intracellular protein kinase cascade Inferred from direct assay Ref.2. Source: UniProtKB mitotic sister chromatid cohesion Inferred from sequence or structural similarity. Source: UniProtKB protein localization to chromosome, centromeric region Inferred from sequence or structural similarity. Source: UniProtKB regulation of spindle checkpoint Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	centrosome Inferred from sequence or structural similarity. Source: UniProtKB chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW nuclear membrane Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay Ref.2. Source: UniProtKB spindle Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from direct assay Ref.2. Source: UniProtKB DNA binding Inferred from direct assay Ref.2. Source: MGI histone kinase activity (H3-T3 specific) Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 754

754

Serine/threonine-protein kinase haspin

PRO_0000085990

Regions

Domain

440 – 754

315

Protein kinase

Nucleotide binding

446 – 454

ATP By similarity UniProtKB P24941

Nucleotide binding

562 – 567

ATP By similarity

Nucleotide binding

605 – 610

ATP By similarity

Nucleotide binding

643 – 645

ATP By similarity

Sites

Active site

127

Proton acceptor By similarity UniProtKB P24941

Active site

605

Proton acceptor By similarity

Binding site

467

ATP By similarity UniProtKB P24941

Amino acid modifications

Modified residue

Phosphoserine By similarity

Experimental info

Sequence conflict

104

P → L in BAA75494. Ref.1

Sequence conflict

104

P → L in CAI24789. Ref.5

Sequence conflict

305

Q → R in BAB00640. Ref.2

Sequence conflict

305

Q → R in AAK30301. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

Q9Z0R0 [UniParc].

Last modified March 29, 2005. Version 3.
Checksum: 4D093D7C95E65308
Show »

FASTA

754

84,181

        10         20         30         40         50         60 
MAQAHPRSGT RLFRTYAARG VRGSQRQPGG LAEQWFQPPN LKRAFSSSLS DSNESPAVAS 

        70         80         90        100        110        120 
DDPDDPDFPG SLVGQRRRRP RGSGSRNQRT LTNTPRVQRL RPRPPQKCST PCSRLRPPPF 

       130        140        150        160        170        180 
PNCSPGCLGS DHSVCIQSRD SNELGTSASL FSSPASPGAP DPLYADSAVP GSFHLPAASL 

       190        200        210        220        230        240 
SEPSVPCPQV AATGDRYTGR ALRAEASFRS SLFSLVNSGA TEENKFGTDG ENVKESCCER 

       250        260        270        280        290        300 
RQQMGNRLTD PDLTSPGKRK AACKKVVSQG VDQRDYEESS ACKDLRVPGE ISRPKRTGPL 

       310        320        330        340        350        360 
RKRKQQEATG TPPRHYHQSK KKRKASVSLW NLNTSQRDSW TKTRASFGFH KKKIITSVIE 

       370        380        390        400        410        420 
VCSSVASSSS RSLLSECSTP PIKNRAHLTV SSRCSSVYLL SPLKTLHVTD QRPSYAEKVY 

       430        440        450        460        470        480 
GECNQEGPIP FSDCLSTEKL ERCEKIGEGV FGEVFQIIND QAPVALKIIA IEGLDLVNGS 

       490        500        510        520        530        540 
HQKTFEEILP EIIISKELSL LSSEAYNRTE GFIGLNSVHC VQGLYPPLLL KAWDHYNTTK 

       550        560        570        580        590        600 
RSANDRPDFF QEDQLFIILE FEFGGVDLER MKTKLSSVAT AKSILHQITA SLAVAEASLH 

       610        620        630        640        650        660 
FEHRDLHWGN VLLKKTNLKE LRYTLNGKTS TIPTHGLQVN IIDYTLSRLE RDGIVVFCDI 

       670        680        690        700        710        720 
SAEEDLFTGE GDYQFEIYRL MRKENKNCWG EYHPYNNVLW LHYLTDKILN KMKFKTKCQS 

       730        740        750 
AAMKQIRKNL QHFHRTVLSF SSATDLLCQH SLFR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of cDNA clones specifically expressed in testicular germ cells." Tanaka H., Yoshimura Y., Nishina Y., Nozaki M., Nojima H., Nishimune Y. FEBS Lett. 355:4-10(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Testis.
[2]	"Identification and characterization of a haploid germ cell-specific nuclear protein kinase (Haspin) in spermatid nuclei and its effects on somatic cells." Tanaka H., Yoshimura Y., Nozaki M., Yomogida K., Tsuchida J., Tosaka Y., Habu T., Nakanishi T., Okada M., Nojima H., Nishimune Y. J. Biol. Chem. 274:17049-17057(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Strain: C57BL/6. Tissue: Testis.
[3]	"Nested genomic structure of haploid germ cell specific haspin gene." Yoshimura Y., Tanaka H., Nozaki M., Yomogida K., Yasunaga T., Nishimune Y. Gene 267:49-54(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The Haspin gene: location in an intron of the Integrin Alpha-E gene, associated transcription of an Integrin alpha-E-derived RNA and expression in diploid as well as haploid cells." Higgins J.M.G. Gene 267:55-69(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY. Strain: 129/Sv.
[5]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[6]	"The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment." Dai J., Sultan S., Taylor S.S., Higgins J.M.G. Genes Dev. 19:472-488(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87326 mRNA. Translation: BAA75494.1. AB036930 Genomic DNA. Translation: BAB00640.1. AF289866 Genomic DNA. Translation: AAK30301.1. AL670399 Genomic DNA. Translation: CAI24789.1.
IPI	IPI00129351.
RefSeq	NP_034483.1. NM_010353.2.
UniGene	Mm.42045.
3D structure databases
ProteinModelPortal	Q9Z0R0.
SMR	Q9Z0R0. Positions 427-754.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000055806.
PTM databases
PhosphoSite	Q9Z0R0.
Proteomic databases
PRIDE	Q9Z0R0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000052140; ENSMUSP00000055806; ENSMUSG00000050107.
GeneID	14841.
KEGG	mmu:14841.
UCSC	uc007jzz.2. mouse.
Organism-specific databases
CTD	83903.
MGI	MGI:1194498. Gsg2.
Phylogenomic databases
eggNOG	COG5072.
GeneTree	ENSGT00390000013015.
HOGENOM	HOG000168256.
HOVERGEN	HBG049009.
InParanoid	Q9Z0R0.
KO	K16315.
OrthoDB	EOG4548Z0.
Gene expression databases
ArrayExpress	Q9Z0R0.
Bgee	Q9Z0R0.
CleanEx	MM_GSG2.
Genevestigator	Q9Z0R0.
GermOnline	ENSMUSG00000050107. Mus musculus.
Family and domain databases
InterPro	IPR024604. DUF3635. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. [Graphical view]
Pfam	PF12330. DUF3635. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	287063.
SOURCE	Search...

Entry information

Entry name

HASP_MOUSE

Accession

Primary (citable) accession number: Q9Z0R0
Secondary accession number(s): Q99MU9, Q9JJJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 99 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents