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Q9Z0R0 (HASP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase haspin

EC=2.7.11.1
Alternative name(s):
Germ cell-specific gene 2 protein
Haploid germ cell-specific nuclear protein kinase
Gene names
Name:Gsg2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length754 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine-protein kinase that phosphorylates histone H3 at 'Ser-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle. Ref.2 Ref.6

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.2

Cofactor

Magnesium. Ref.2

Enzyme regulation

Constitutive activity that does not require phosphorylation. Specifically inhibited by 3-(1H-indazol-5-yl)-N-propylimidazo[1,2-b]pyridazin-6-amine (CHR-6494) By similarity.

Subcellular location

Nucleus. Chromosome By similarity. Cytoplasmcytoskeletonspindle By similarity. Note: Nuclear during interphase and associates with the chromosomes and spindle apparatus during mitosis By similarity. Ref.2

Tissue specificity

Expressed in germ cells within the testis of adults and of embryos from day 24 onwards. Also present in adult thymus and weakly expressed in spleen, lung and whole embryo. Ref.1 Ref.2 Ref.4

Post-translational modification

Autophosphorylated on both serine and threonine residues By similarity. Strongly phosphorylated during mitosis but this does not appear to significantly affect its intrinsic kinase activity. Phosphorylation by AURKB is required for full activity toward histone H3 at 'Ser-3' in mitosis By similarity. Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processCell cycle
   Cellular componentChromosome
Cytoplasm
Cytoskeleton
Nucleus
   LigandATP-binding
Magnesium
Nucleotide-binding
   Molecular functionChromatin regulator
Kinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processhistone H3-T3 phosphorylation involved in chromosome passenger complex localization to kinetochore

Inferred from sequence or structural similarity. Source: UniProtKB

intracellular signal transduction

Inferred from direct assay Ref.2. Source: UniProtKB

mitotic sister chromatid cohesion

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of G1/S transition of mitotic cell cycle

Inferred from direct assay Ref.2. Source: MGI

protein localization to chromosome, centromeric region

Inferred from sequence or structural similarity. Source: UniProtKB

protein phosphorylation

Inferred from direct assay Ref.2. Source: UniProtKB

regulation of spindle checkpoint

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcentrosome

Inferred from sequence or structural similarity. Source: UniProtKB

chromosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

nuclear membrane

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay Ref.2. Source: UniProtKB

spindle

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from direct assay Ref.2. Source: UniProtKB

DNA binding

Inferred from direct assay Ref.2. Source: MGI

histone kinase activity (H3-T3 specific)

Inferred from sequence or structural similarity. Source: UniProtKB

protein serine/threonine kinase activity

Inferred from direct assay Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 754754Serine/threonine-protein kinase haspin
PRO_0000085990

Regions

Domain440 – 754315Protein kinase
Nucleotide binding446 – 4549ATP By similarity UniProtKB P24941
Nucleotide binding562 – 5676ATP By similarity
Nucleotide binding605 – 6106ATP By similarity
Nucleotide binding643 – 6453ATP By similarity

Sites

Active site1271Proton acceptor By similarity UniProtKB P24941
Active site6051Proton acceptor By similarity
Binding site4671ATP By similarity UniProtKB P24941

Amino acid modifications

Modified residue551Phosphoserine By similarity

Experimental info

Sequence conflict1041P → L in BAA75494. Ref.1
Sequence conflict1041P → L in CAI24789. Ref.5
Sequence conflict3051Q → R in BAB00640. Ref.2
Sequence conflict3051Q → R in AAK30301. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9Z0R0 [UniParc].

Last modified March 29, 2005. Version 3.
Checksum: 4D093D7C95E65308

FASTA75484,181
        10         20         30         40         50         60 
MAQAHPRSGT RLFRTYAARG VRGSQRQPGG LAEQWFQPPN LKRAFSSSLS DSNESPAVAS 

        70         80         90        100        110        120 
DDPDDPDFPG SLVGQRRRRP RGSGSRNQRT LTNTPRVQRL RPRPPQKCST PCSRLRPPPF 

       130        140        150        160        170        180 
PNCSPGCLGS DHSVCIQSRD SNELGTSASL FSSPASPGAP DPLYADSAVP GSFHLPAASL 

       190        200        210        220        230        240 
SEPSVPCPQV AATGDRYTGR ALRAEASFRS SLFSLVNSGA TEENKFGTDG ENVKESCCER 

       250        260        270        280        290        300 
RQQMGNRLTD PDLTSPGKRK AACKKVVSQG VDQRDYEESS ACKDLRVPGE ISRPKRTGPL 

       310        320        330        340        350        360 
RKRKQQEATG TPPRHYHQSK KKRKASVSLW NLNTSQRDSW TKTRASFGFH KKKIITSVIE 

       370        380        390        400        410        420 
VCSSVASSSS RSLLSECSTP PIKNRAHLTV SSRCSSVYLL SPLKTLHVTD QRPSYAEKVY 

       430        440        450        460        470        480 
GECNQEGPIP FSDCLSTEKL ERCEKIGEGV FGEVFQIIND QAPVALKIIA IEGLDLVNGS 

       490        500        510        520        530        540 
HQKTFEEILP EIIISKELSL LSSEAYNRTE GFIGLNSVHC VQGLYPPLLL KAWDHYNTTK 

       550        560        570        580        590        600 
RSANDRPDFF QEDQLFIILE FEFGGVDLER MKTKLSSVAT AKSILHQITA SLAVAEASLH 

       610        620        630        640        650        660 
FEHRDLHWGN VLLKKTNLKE LRYTLNGKTS TIPTHGLQVN IIDYTLSRLE RDGIVVFCDI 

       670        680        690        700        710        720 
SAEEDLFTGE GDYQFEIYRL MRKENKNCWG EYHPYNNVLW LHYLTDKILN KMKFKTKCQS 

       730        740        750 
AAMKQIRKNL QHFHRTVLSF SSATDLLCQH SLFR 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of cDNA clones specifically expressed in testicular germ cells."
Tanaka H., Yoshimura Y., Nishina Y., Nozaki M., Nojima H., Nishimune Y.
FEBS Lett. 355:4-10(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Testis.
[2]"Identification and characterization of a haploid germ cell-specific nuclear protein kinase (Haspin) in spermatid nuclei and its effects on somatic cells."
Tanaka H., Yoshimura Y., Nozaki M., Yomogida K., Tsuchida J., Tosaka Y., Habu T., Nakanishi T., Okada M., Nojima H., Nishimune Y.
J. Biol. Chem. 274:17049-17057(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Strain: C57BL/6.
Tissue: Testis.
[3]"Nested genomic structure of haploid germ cell specific haspin gene."
Yoshimura Y., Tanaka H., Nozaki M., Yomogida K., Yasunaga T., Nishimune Y.
Gene 267:49-54(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The Haspin gene: location in an intron of the Integrin Alpha-E gene, associated transcription of an Integrin alpha-E-derived RNA and expression in diploid as well as haploid cells."
Higgins J.M.G.
Gene 267:55-69(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
Strain: 129/Sv.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]"The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment."
Dai J., Sultan S., Taylor S.S., Higgins J.M.G.
Genes Dev. 19:472-488(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87326 mRNA. Translation: BAA75494.1.
AB036930 Genomic DNA. Translation: BAB00640.1.
AF289866 Genomic DNA. Translation: AAK30301.1.
AL670399 Genomic DNA. Translation: CAI24789.1.
RefSeqNP_034483.1. NM_010353.2.
UniGeneMm.42045.

3D structure databases

ProteinModelPortalQ9Z0R0.
SMRQ9Z0R0. Positions 426-754.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9Z0R0. 1 interaction.
STRING10090.ENSMUSP00000055806.

PTM databases

PhosphoSiteQ9Z0R0.

Proteomic databases

PRIDEQ9Z0R0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000052140; ENSMUSP00000055806; ENSMUSG00000050107.
GeneID14841.
KEGGmmu:14841.
UCSCuc007jzz.2. mouse.

Organism-specific databases

CTD83903.
MGIMGI:1194498. Gsg2.

Phylogenomic databases

eggNOGCOG5072.
GeneTreeENSGT00390000013015.
HOGENOMHOG000168256.
HOVERGENHBG049009.
InParanoidQ9Z0R0.
KOK16315.
OrthoDBEOG744T89.
PhylomeDBQ9Z0R0.
TreeFamTF313895.

Gene expression databases

ArrayExpressQ9Z0R0.
BgeeQ9Z0R0.
CleanExMM_GSG2.
GenevestigatorQ9Z0R0.

Family and domain databases

InterProIPR024604. DUF3635.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamPF12330. DUF3635. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287063.
PROQ9Z0R0.
SOURCESearch...

Entry information

Entry nameHASP_MOUSE
AccessionPrimary (citable) accession number: Q9Z0R0
Secondary accession number(s): Q99MU9, Q9JJJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 29, 2005
Last modified: April 16, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot