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Protein

Serine/threonine-protein kinase haspin

Gene

Gsg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that phosphorylates histone H3 at 'Ser-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+1 Publication

Enzyme regulationi

Constitutive activity that does not require phosphorylation. Specifically inhibited by 3-(1H-indazol-5-yl)-N-propylimidazo[1,2-b]pyridazin-6-amine (CHR-6494) (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation
Binding sitei467 – 4671ATPPROSITE-ProRule annotationBy similarity
Active sitei605 – 6051Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi446 – 4549ATPPROSITE-ProRule annotationBy similarity
Nucleotide bindingi562 – 5676ATPPROSITE-ProRule annotation
Nucleotide bindingi605 – 6106ATPPROSITE-ProRule annotation
Nucleotide bindingi643 – 6453ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. DNA binding Source: MGI
  3. histone kinase activity (H3-T3 specific) Source: UniProtKB
  4. protein kinase activity Source: MGI
  5. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. histone H3-T3 phosphorylation involved in chromosome passenger complex localization to kinetochore Source: UniProtKB
  2. intracellular signal transduction Source: UniProtKB
  3. mitotic sister chromatid cohesion Source: UniProtKB
  4. negative regulation of G1/S transition of mitotic cell cycle Source: MGI
  5. protein localization to chromosome, centromeric region Source: UniProtKB
  6. protein phosphorylation Source: UniProtKB
  7. regulation of spindle checkpoint Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase haspin (EC:2.7.11.1)
Alternative name(s):
Germ cell-specific gene 2 protein
Haploid germ cell-specific nuclear protein kinase
Gene namesi
Name:Gsg2
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1194498. Gsg2.

Subcellular locationi

Nucleus 1 Publication. Chromosome By similarity. Cytoplasmcytoskeletonspindle By similarity
Note: Nuclear during interphase and associates with the chromosomes and spindle apparatus during mitosis.By similarity

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. chromosome Source: UniProtKB-SubCell
  3. cytoplasm Source: GO_Central
  4. nucleus Source: UniProtKB
  5. spindle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Serine/threonine-protein kinase haspinPRO_0000085990Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei55 – 551PhosphoserineBy similarity

Post-translational modificationi

Autophosphorylated on both serine and threonine residues (By similarity). Strongly phosphorylated during mitosis but this does not appear to significantly affect its intrinsic kinase activity. Phosphorylation by AURKB is required for full activity toward histone H3 at 'Ser-3' in mitosis (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z0R0.
PRIDEiQ9Z0R0.

PTM databases

PhosphoSiteiQ9Z0R0.

Expressioni

Tissue specificityi

Expressed in germ cells within the testis of adults and of embryos from day 24 onwards. Also present in adult thymus and weakly expressed in spleen, lung and whole embryo.3 Publications

Gene expression databases

BgeeiQ9Z0R0.
CleanExiMM_GSG2.
ExpressionAtlasiQ9Z0R0. baseline and differential.
GenevestigatoriQ9Z0R0.

Interactioni

Protein-protein interaction databases

IntActiQ9Z0R0. 1 interaction.
STRINGi10090.ENSMUSP00000055806.

Structurei

3D structure databases

SMRiQ9Z0R0. Positions 425-754.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini440 – 754315Protein kinasePROSITE-ProRule annotationCuratedAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5072.
HOGENOMiHOG000168256.
HOVERGENiHBG049009.
InParanoidiQ9Z0R0.
KOiK16315.
OrthoDBiEOG744T89.
PhylomeDBiQ9Z0R0.
TreeFamiTF313895.

Family and domain databases

InterProiIPR024604. DUF3635.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12330. DUF3635. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z0R0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQAHPRSGT RLFRTYAARG VRGSQRQPGG LAEQWFQPPN LKRAFSSSLS
60 70 80 90 100
DSNESPAVAS DDPDDPDFPG SLVGQRRRRP RGSGSRNQRT LTNTPRVQRL
110 120 130 140 150
RPRPPQKCST PCSRLRPPPF PNCSPGCLGS DHSVCIQSRD SNELGTSASL
160 170 180 190 200
FSSPASPGAP DPLYADSAVP GSFHLPAASL SEPSVPCPQV AATGDRYTGR
210 220 230 240 250
ALRAEASFRS SLFSLVNSGA TEENKFGTDG ENVKESCCER RQQMGNRLTD
260 270 280 290 300
PDLTSPGKRK AACKKVVSQG VDQRDYEESS ACKDLRVPGE ISRPKRTGPL
310 320 330 340 350
RKRKQQEATG TPPRHYHQSK KKRKASVSLW NLNTSQRDSW TKTRASFGFH
360 370 380 390 400
KKKIITSVIE VCSSVASSSS RSLLSECSTP PIKNRAHLTV SSRCSSVYLL
410 420 430 440 450
SPLKTLHVTD QRPSYAEKVY GECNQEGPIP FSDCLSTEKL ERCEKIGEGV
460 470 480 490 500
FGEVFQIIND QAPVALKIIA IEGLDLVNGS HQKTFEEILP EIIISKELSL
510 520 530 540 550
LSSEAYNRTE GFIGLNSVHC VQGLYPPLLL KAWDHYNTTK RSANDRPDFF
560 570 580 590 600
QEDQLFIILE FEFGGVDLER MKTKLSSVAT AKSILHQITA SLAVAEASLH
610 620 630 640 650
FEHRDLHWGN VLLKKTNLKE LRYTLNGKTS TIPTHGLQVN IIDYTLSRLE
660 670 680 690 700
RDGIVVFCDI SAEEDLFTGE GDYQFEIYRL MRKENKNCWG EYHPYNNVLW
710 720 730 740 750
LHYLTDKILN KMKFKTKCQS AAMKQIRKNL QHFHRTVLSF SSATDLLCQH

SLFR
Length:754
Mass (Da):84,181
Last modified:March 28, 2005 - v3
Checksum:i4D093D7C95E65308
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti104 – 1041P → L in BAA75494 (PubMed:7957958).Curated
Sequence conflicti104 – 1041P → L in CAI24789 (PubMed:19468303).Curated
Sequence conflicti305 – 3051Q → R in BAB00640 (PubMed:10358056).Curated
Sequence conflicti305 – 3051Q → R in AAK30301 (PubMed:11311556).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87326 mRNA. Translation: BAA75494.1.
AB036930 Genomic DNA. Translation: BAB00640.1.
AF289866 Genomic DNA. Translation: AAK30301.1.
AL670399 Genomic DNA. Translation: CAI24789.1.
CCDSiCCDS24997.1.
RefSeqiNP_034483.1. NM_010353.2.
UniGeneiMm.42045.

Genome annotation databases

GeneIDi14841.
KEGGimmu:14841.
UCSCiuc007jzz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87326 mRNA. Translation: BAA75494.1.
AB036930 Genomic DNA. Translation: BAB00640.1.
AF289866 Genomic DNA. Translation: AAK30301.1.
AL670399 Genomic DNA. Translation: CAI24789.1.
CCDSiCCDS24997.1.
RefSeqiNP_034483.1. NM_010353.2.
UniGeneiMm.42045.

3D structure databases

SMRiQ9Z0R0. Positions 425-754.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z0R0. 1 interaction.
STRINGi10090.ENSMUSP00000055806.

PTM databases

PhosphoSiteiQ9Z0R0.

Proteomic databases

MaxQBiQ9Z0R0.
PRIDEiQ9Z0R0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi14841.
KEGGimmu:14841.
UCSCiuc007jzz.2. mouse.

Organism-specific databases

CTDi83903.
MGIiMGI:1194498. Gsg2.

Phylogenomic databases

eggNOGiCOG5072.
HOGENOMiHOG000168256.
HOVERGENiHBG049009.
InParanoidiQ9Z0R0.
KOiK16315.
OrthoDBiEOG744T89.
PhylomeDBiQ9Z0R0.
TreeFamiTF313895.

Miscellaneous databases

NextBioi287063.
PROiQ9Z0R0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z0R0.
CleanExiMM_GSG2.
ExpressionAtlasiQ9Z0R0. baseline and differential.
GenevestigatoriQ9Z0R0.

Family and domain databases

InterProiIPR024604. DUF3635.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF12330. DUF3635. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of cDNA clones specifically expressed in testicular germ cells."
    Tanaka H., Yoshimura Y., Nishina Y., Nozaki M., Nojima H., Nishimune Y.
    FEBS Lett. 355:4-10(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/61 Publication.
    Tissue: TestisImported.
  2. "Identification and characterization of a haploid germ cell-specific nuclear protein kinase (Haspin) in spermatid nuclei and its effects on somatic cells."
    Tanaka H., Yoshimura Y., Nozaki M., Yomogida K., Tsuchida J., Tosaka Y., Habu T., Nakanishi T., Okada M., Nojima H., Nishimune Y.
    J. Biol. Chem. 274:17049-17057(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: C57BL/61 Publication.
    Tissue: TestisImported.
  3. "Nested genomic structure of haploid germ cell specific haspin gene."
    Yoshimura Y., Tanaka H., Nozaki M., Yomogida K., Yasunaga T., Nishimune Y.
    Gene 267:49-54(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The Haspin gene: location in an intron of the Integrin Alpha-E gene, associated transcription of an Integrin alpha-E-derived RNA and expression in diploid as well as haploid cells."
    Higgins J.M.G.
    Gene 267:55-69(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/SvImported.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. "The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment."
    Dai J., Sultan S., Taylor S.S., Higgins J.M.G.
    Genes Dev. 19:472-488(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiHASP_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0R0
Secondary accession number(s): Q99MU9, Q9JJJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 29, 2004
Last sequence update: March 28, 2005
Last modified: March 31, 2015
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.