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Q9Z0R0

- HASP_MOUSE

UniProt

Q9Z0R0 - HASP_MOUSE

Protein

Serine/threonine-protein kinase haspin

Gene

Gsg2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 3 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Serine/threonine-protein kinase that phosphorylates histone H3 at 'Ser-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.1 Publication

    Enzyme regulationi

    Constitutive activity that does not require phosphorylation. Specifically inhibited by 3-(1H-indazol-5-yl)-N-propylimidazo[1,2-b]pyridazin-6-amine (CHR-6494) By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation
    Binding sitei467 – 4671ATPBy similarityPROSITE-ProRule annotation
    Active sitei605 – 6051Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi446 – 4549ATPBy similarityPROSITE-ProRule annotation
    Nucleotide bindingi562 – 5676ATPPROSITE-ProRule annotation
    Nucleotide bindingi605 – 6106ATPPROSITE-ProRule annotation
    Nucleotide bindingi643 – 6453ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. DNA binding Source: MGI
    3. histone kinase activity (H3-T3 specific) Source: UniProtKB
    4. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. histone H3-T3 phosphorylation involved in chromosome passenger complex localization to kinetochore Source: UniProtKB
    2. intracellular signal transduction Source: UniProtKB
    3. mitotic sister chromatid cohesion Source: UniProtKB
    4. negative regulation of G1/S transition of mitotic cell cycle Source: MGI
    5. protein localization to chromosome, centromeric region Source: UniProtKB
    6. protein phosphorylation Source: UniProtKB
    7. regulation of spindle checkpoint Source: UniProtKB

    Keywords - Molecular functioni

    Chromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase haspin (EC:2.7.11.1)
    Alternative name(s):
    Germ cell-specific gene 2 protein
    Haploid germ cell-specific nuclear protein kinase
    Gene namesi
    Name:Gsg2
    OrganismiMus musculus (Mouse)Imported
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1194498. Gsg2.

    Subcellular locationi

    Nucleus 1 Publication. Chromosome By similarity. Cytoplasmcytoskeletonspindle By similarity
    Note: Nuclear during interphase and associates with the chromosomes and spindle apparatus during mitosis.By similarity

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. chromosome Source: UniProtKB-SubCell
    3. cytoplasm Source: UniProtKB-KW
    4. nuclear membrane Source: Ensembl
    5. nucleus Source: UniProtKB
    6. spindle Source: UniProtKB

    Keywords - Cellular componenti

    Chromosome, Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 754754Serine/threonine-protein kinase haspinPRO_0000085990Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei55 – 551PhosphoserineBy similarity

    Post-translational modificationi

    Autophosphorylated on both serine and threonine residues By similarity. Strongly phosphorylated during mitosis but this does not appear to significantly affect its intrinsic kinase activity. Phosphorylation by AURKB is required for full activity toward histone H3 at 'Ser-3' in mitosis By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9Z0R0.

    PTM databases

    PhosphoSiteiQ9Z0R0.

    Expressioni

    Tissue specificityi

    Expressed in germ cells within the testis of adults and of embryos from day 24 onwards. Also present in adult thymus and weakly expressed in spleen, lung and whole embryo.3 Publications

    Gene expression databases

    ArrayExpressiQ9Z0R0.
    BgeeiQ9Z0R0.
    CleanExiMM_GSG2.
    GenevestigatoriQ9Z0R0.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9Z0R0. 1 interaction.
    STRINGi10090.ENSMUSP00000055806.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z0R0.
    SMRiQ9Z0R0. Positions 427-754.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini440 – 754315Protein kinaseCuratedPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5072.
    GeneTreeiENSGT00390000013015.
    HOGENOMiHOG000168256.
    HOVERGENiHBG049009.
    InParanoidiQ9Z0R0.
    KOiK16315.
    OrthoDBiEOG744T89.
    PhylomeDBiQ9Z0R0.
    TreeFamiTF313895.

    Family and domain databases

    InterProiIPR024604. DUF3635.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    [Graphical view]
    PfamiPF12330. DUF3635. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z0R0-1 [UniParc]FASTAAdd to Basket

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    MAQAHPRSGT RLFRTYAARG VRGSQRQPGG LAEQWFQPPN LKRAFSSSLS    50
    DSNESPAVAS DDPDDPDFPG SLVGQRRRRP RGSGSRNQRT LTNTPRVQRL 100
    RPRPPQKCST PCSRLRPPPF PNCSPGCLGS DHSVCIQSRD SNELGTSASL 150
    FSSPASPGAP DPLYADSAVP GSFHLPAASL SEPSVPCPQV AATGDRYTGR 200
    ALRAEASFRS SLFSLVNSGA TEENKFGTDG ENVKESCCER RQQMGNRLTD 250
    PDLTSPGKRK AACKKVVSQG VDQRDYEESS ACKDLRVPGE ISRPKRTGPL 300
    RKRKQQEATG TPPRHYHQSK KKRKASVSLW NLNTSQRDSW TKTRASFGFH 350
    KKKIITSVIE VCSSVASSSS RSLLSECSTP PIKNRAHLTV SSRCSSVYLL 400
    SPLKTLHVTD QRPSYAEKVY GECNQEGPIP FSDCLSTEKL ERCEKIGEGV 450
    FGEVFQIIND QAPVALKIIA IEGLDLVNGS HQKTFEEILP EIIISKELSL 500
    LSSEAYNRTE GFIGLNSVHC VQGLYPPLLL KAWDHYNTTK RSANDRPDFF 550
    QEDQLFIILE FEFGGVDLER MKTKLSSVAT AKSILHQITA SLAVAEASLH 600
    FEHRDLHWGN VLLKKTNLKE LRYTLNGKTS TIPTHGLQVN IIDYTLSRLE 650
    RDGIVVFCDI SAEEDLFTGE GDYQFEIYRL MRKENKNCWG EYHPYNNVLW 700
    LHYLTDKILN KMKFKTKCQS AAMKQIRKNL QHFHRTVLSF SSATDLLCQH 750
    SLFR 754
    Length:754
    Mass (Da):84,181
    Last modified:March 29, 2005 - v3
    Checksum:i4D093D7C95E65308
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti104 – 1041P → L in BAA75494. (PubMed:7957958)Curated
    Sequence conflicti104 – 1041P → L in CAI24789. (PubMed:19468303)Curated
    Sequence conflicti305 – 3051Q → R in BAB00640. (PubMed:10358056)Curated
    Sequence conflicti305 – 3051Q → R in AAK30301. (PubMed:11311556)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87326 mRNA. Translation: BAA75494.1.
    AB036930 Genomic DNA. Translation: BAB00640.1.
    AF289866 Genomic DNA. Translation: AAK30301.1.
    AL670399 Genomic DNA. Translation: CAI24789.1.
    CCDSiCCDS24997.1.
    RefSeqiNP_034483.1. NM_010353.2.
    UniGeneiMm.42045.

    Genome annotation databases

    EnsembliENSMUST00000052140; ENSMUSP00000055806; ENSMUSG00000050107.
    GeneIDi14841.
    KEGGimmu:14841.
    UCSCiuc007jzz.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87326 mRNA. Translation: BAA75494.1 .
    AB036930 Genomic DNA. Translation: BAB00640.1 .
    AF289866 Genomic DNA. Translation: AAK30301.1 .
    AL670399 Genomic DNA. Translation: CAI24789.1 .
    CCDSi CCDS24997.1.
    RefSeqi NP_034483.1. NM_010353.2.
    UniGenei Mm.42045.

    3D structure databases

    ProteinModelPortali Q9Z0R0.
    SMRi Q9Z0R0. Positions 427-754.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9Z0R0. 1 interaction.
    STRINGi 10090.ENSMUSP00000055806.

    PTM databases

    PhosphoSitei Q9Z0R0.

    Proteomic databases

    PRIDEi Q9Z0R0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000052140 ; ENSMUSP00000055806 ; ENSMUSG00000050107 .
    GeneIDi 14841.
    KEGGi mmu:14841.
    UCSCi uc007jzz.2. mouse.

    Organism-specific databases

    CTDi 83903.
    MGIi MGI:1194498. Gsg2.

    Phylogenomic databases

    eggNOGi COG5072.
    GeneTreei ENSGT00390000013015.
    HOGENOMi HOG000168256.
    HOVERGENi HBG049009.
    InParanoidi Q9Z0R0.
    KOi K16315.
    OrthoDBi EOG744T89.
    PhylomeDBi Q9Z0R0.
    TreeFami TF313895.

    Miscellaneous databases

    NextBioi 287063.
    PROi Q9Z0R0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z0R0.
    Bgeei Q9Z0R0.
    CleanExi MM_GSG2.
    Genevestigatori Q9Z0R0.

    Family and domain databases

    InterProi IPR024604. DUF3635.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    [Graphical view ]
    Pfami PF12330. DUF3635. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of cDNA clones specifically expressed in testicular germ cells."
      Tanaka H., Yoshimura Y., Nishina Y., Nozaki M., Nojima H., Nishimune Y.
      FEBS Lett. 355:4-10(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: C57BL/61 Publication.
      Tissue: TestisImported.
    2. "Identification and characterization of a haploid germ cell-specific nuclear protein kinase (Haspin) in spermatid nuclei and its effects on somatic cells."
      Tanaka H., Yoshimura Y., Nozaki M., Yomogida K., Tsuchida J., Tosaka Y., Habu T., Nakanishi T., Okada M., Nojima H., Nishimune Y.
      J. Biol. Chem. 274:17049-17057(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PHOSPHORYLATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: C57BL/61 Publication.
      Tissue: TestisImported.
    3. "Nested genomic structure of haploid germ cell specific haspin gene."
      Yoshimura Y., Tanaka H., Nozaki M., Yomogida K., Yasunaga T., Nishimune Y.
      Gene 267:49-54(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The Haspin gene: location in an intron of the Integrin Alpha-E gene, associated transcription of an Integrin alpha-E-derived RNA and expression in diploid as well as haploid cells."
      Higgins J.M.G.
      Gene 267:55-69(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
      Strain: 129/SvImported.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    6. "The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment."
      Dai J., Sultan S., Taylor S.S., Higgins J.M.G.
      Genes Dev. 19:472-488(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiHASP_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z0R0
    Secondary accession number(s): Q99MU9, Q9JJJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 110 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3