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Protein

Potassium voltage-gated channel subfamily KQT member 1

Gene

Kcnq1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon (By similarity). Associates with KCNE beta subunits that modulates current kinetics (By similarity) (PubMed:21911611). Induces a voltage-dependent by rapidly activating and slowly deactivating potassium-selective outward current (By similarity) (PubMed:11220365). Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (PubMed:11220365). During beta-adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks) (By similarity) (PubMed:11220365). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (By similarity). When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (By similarity). This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (PubMed:21911611). During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (By similarity). Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (By similarity). When associated with KCNE2, forms a heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (By similarity). When associated with KCNE4, inhibits voltage-gated potassium channel activity (By similarity). When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (By similarity). Also forms a heterotetramer with KCNQ5 that has a voltage-gated potassium channel activity (By similarity). Binds with phosphatidylinositol 4,5-bisphosphate (By similarity).By similarity2 Publications

GO - Molecular functioni

  • delayed rectifier potassium channel activity Source: UniProtKB
  • outward rectifier potassium channel activity Source: UniProtKB
  • phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB
  • potassium channel activity Source: RGD
  • protein homodimerization activity Source: RGD

GO - Biological processi

  • inner ear development Source: UniProtKB
  • intestinal absorption Source: UniProtKB
  • male gonad development Source: RGD
  • membrane repolarization Source: UniProtKB
  • negative regulation of insulin secretion Source: RGD
  • positive regulation of gastric acid secretion Source: RGD
  • potassium ion transport Source: RGD
  • regulation of gastric acid secretion Source: UniProtKB
  • regulation of membrane potential Source: RGD
  • renal absorption Source: UniProtKB
  • response to anesthetic Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Calmodulin-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily KQT member 1Curated
Alternative name(s):
IKs producing slow voltage-gated potassium channel subunit alpha KvLQT11 Publication
KQT-like 1By similarity
Voltage-gated potassium channel subunit Kv7.1By similarity
Gene namesi
Name:Kcnq1Imported
Synonyms:Kcna9By similarity, Kvlqt11 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621503. Kcnq1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Cytoplasmic vesicle membrane By similarity
  • Early endosome 1 Publication
  • Membrane raft By similarity
  • Endoplasmic reticulum By similarity
  • Basolateral cell membrane By similarity

  • Note: Colocalized with KCNE3 at the plasma membrane. Upon 17beta-oestradiol treatment, colocalizes with RAB5A at early endosome. Heterotetramer with KCNQ5 is highly retained at the endoplasmic reticulum and is localized outside of lipid raft microdomains. During the early stages of epithelial cell polarization induced by the calcium switch it removed from plasma membrane to the endoplasmic reticulum where it retained and it is redistributed to the basolateral cell surface in a PI3K-dependent manner at a later stage.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei122 – 14221Helical; Name=Segment S1Sequence analysisAdd
BLAST
Topological domaini143 – 1475ExtracellularSequence analysis
Transmembranei148 – 16821Helical; Name=Segment S2Sequence analysisAdd
BLAST
Topological domaini169 – 19628CytoplasmicSequence analysisAdd
BLAST
Transmembranei197 – 21721Helical; Name=Segment S3Sequence analysisAdd
BLAST
Topological domaini218 – 2258ExtracellularSequence analysis
Transmembranei226 – 24823Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd
BLAST
Topological domaini249 – 26113CytoplasmicSequence analysisAdd
BLAST
Transmembranei262 – 28221Helical; Name=Segment S5Sequence analysisAdd
BLAST
Topological domaini283 – 29917ExtracellularSequence analysisAdd
BLAST
Intramembranei300 – 32021Pore-forming; Name=Segment H5Sequence analysisAdd
BLAST
Topological domaini321 – 33111ExtracellularSequence analysisAdd
BLAST
Transmembranei332 – 35221Helical; Name=Segment S6Sequence analysisAdd
BLAST
Topological domaini353 – 669317CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: UniProtKB
  • cytoplasmic vesicle membrane Source: RGD
  • early endosome Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: UniProtKB
  • integral component of plasma membrane Source: RGD
  • membrane raft Source: UniProtKB
  • sarcolemma Source: RGD
  • voltage-gated potassium channel complex Source: RGD
  • zymogen granule membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 669669Potassium voltage-gated channel subfamily KQT member 1PRO_0000054027Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271Phosphoserine; by PKABy similarity
Glycosylationi289 – 2891N-linked (GlcNAc...)Sequence analysis
Modified residuei407 – 4071PhosphoserineBy similarity
Modified residuei409 – 4091PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation at Ser-27 by PKA; increases delayed rectifier potassium channel activity of the KCNQ1-KCNE1 complex through a macromolecular complex that includes PKA, PP1, and the targeting protein AKAP9.By similarity
Ubiquitinated by NEDD4L; promotes internalization. The ubiquitinylated form is internalized through a clathrin-mediated endocytosis by interacting with AP2M1 and is recycled back to the cell membrane via RAB4A and RAB11A.By similarity
Deubiquitinated by USP2; counteracts the NEDD4L-specific down-regulation of I(Ks) and restores the membrane localization.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9Z0N7.

Expressioni

Gene expression databases

BgeeiENSRNOG00000020532.

Interactioni

Subunit structurei

Tetramer. Heterotetramer with KCNE1; targets to the membrane raft. Interacts (via C-terminus) with CALM; forms an heterotetramer in a calcium-independent manner. Interacts with AKAP9; targets protein kinase A (PKA) catalytic and regulatory subunits and protein phosphatase 1 (PP1) to the KCNQ1-KCNE1 complex, allowing PKA-mediated phosphorylation and increase of delayed rectifier potassium channel activity. Interacts with KCNE2; form a heterooligomer complex that targets to the membrane raft and leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current. Interacts with AP2M1; mediates estrogen-induced internalization via clathrin-coated vesicles. Interacts with NEDD4L; promotes internalization and decreases I(Ks) currents. Interacts with USP2; counteracts the NEDD4L-specific down-regulation of I(Ks) and restore plasma membrane localization. Heterotetramer with KCNQ5; has a voltage-gated potassium channel activity (By similarity). Interacts with KCNE3; produces a current with nearly instantaneous activation with a linear current-voltage relationship and alters membrane raft localization (By similarity) (PubMed:21911611). Interacts with KCNE4; impairs KCNQ1 localization in lipid rafts and inhibits voltage-gated potassium channel activity. Interacts with KCNE5; impairs KCNQ1 localization in lipid rafts and only conducts current upon strong and continued depolarization (By similarity).By similarity1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

BioGridi249886. 1 interaction.
STRINGi10116.ENSRNOP00000027875.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0N7.
SMRiQ9Z0N7. Positions 584-620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni535 – 57238Interaction with KCNE1 C-terminusBy similarityAdd
BLAST
Regioni588 – 61629Interaction with AKAP9By similarityAdd
BLAST
Regioni589 – 62032C-terminal assembly domainBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili585 – 62137By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi312 – 3176Selectivity filterBy similarity

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.By similarity
The coiled-coil domain mediates tetramerization.By similarity
The segment S6 is involved in the inhibition of voltage-gated potassium channel activity by KCNE4.By similarity
The C-terminal assembly domain promotes self-interactiona; allows functional channel.By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1419. Eukaryota.
COG1226. LUCA.
HOGENOMiHOG000220839.
HOVERGENiHBG059014.
InParanoidiQ9Z0N7.
KOiK04926.
PhylomeDBiQ9Z0N7.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
IPR003937. K_chnl_volt-dep_KCNQ.
IPR013821. K_chnl_volt-dep_KCNQ_C.
IPR005827. K_chnl_volt-dep_KCQN1.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 2 hits.
PTHR11537:SF151. PTHR11537:SF151. 2 hits.
PfamiPF00520. Ion_trans. 1 hit.
PF03520. KCNQ_channel. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01460. KCNQ1CHANNEL.
PR01459. KCNQCHANNEL.

Sequencei

Sequence statusi: Complete.

Q9Z0N7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTASSPPNA ERKRAGWGRL LGARRGSAGL AKKCPFSLEL AEGGPTGGTV
60 70 80 90 100
YAPIAPTGAP GLAPPMSPPV SPVPAPADLG PRPRVSLDPR VSIYSTRRPL
110 120 130 140 150
LARTHIQGRV YNFLERPTGW KCFVYHFTVF LIVLVCLIFS VLSTIEQYAA
160 170 180 190 200
LATGTLFWME IVLVVFFGTE YVVRLWSAGC RSKYVGIWGR LRFARKPISI
210 220 230 240 250
IDLIVVVASM VVLCVGSKGQ VFATSAIRGI RFLQILRMLH VDRQGGTWRL
260 270 280 290 300
LGSVVFIHRQ ELITTLYIGF LGLIFSSYFV YLAEKDAVNE SGRIEFGSYA
310 320 330 340 350
DALWWGVVTV TTIGYGDKVP QTWVGKTIAS CFSVFAISFF ALPAGILGSG
360 370 380 390 400
FALKVQQKQR QKHFNRQIPA AASLIQTAWR CYAAENPDSS TWKIYVRKPA
410 420 430 440 450
RSHTLLSPSP KPKKSVMVKK KKFKLDKDNG LSPGEKIFNV PHITCDPPED
460 470 480 490 500
RRPDHFSIDG YDSSVRKSPT LLEVSTPHFL RTNSFAEDLD LEGETLLTPI
510 520 530 540 550
THVSQLRDHH RATIKVIRRM QYFVAKKKFQ QARKPYDVRD VIEQYSQGHL
560 570 580 590 600
NLMVRIKELQ RRLDQSIGKP SLFIPISEKS KDRGSNTIGA RLNRVEDKVT
610 620 630 640 650
QLDQRLVIIT DMLHQLLSLQ QGGPTCNNRS QVVASDERGS INPELFLPSN
660
SLPTYEQLTV PQTGPDEGS
Length:669
Mass (Da):74,579
Last modified:May 1, 1999 - v1
Checksum:i2374CA1290020939
GO

Sequence cautioni

The sequence AAB51395 differs from that shown. Reason: Frameshift at position 321. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1701E → K in AAB51395 (PubMed:9314834).Curated
Sequence conflicti182 – 1821S → T in AAB51395 (PubMed:9314834).Curated
Sequence conflicti185 – 1851V → L in AAB51395 (PubMed:9314834).Curated
Sequence conflicti255 – 2551V → F in AAB51395 (PubMed:9314834).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133685 mRNA. Translation: CAB38863.1.
U92655 mRNA. Translation: AAB51395.1. Frameshift.
RefSeqiNP_114462.1. NM_032073.1.
UniGeneiRn.9779.

Genome annotation databases

GeneIDi84020.
KEGGirno:84020.
UCSCiRGD:621503. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133685 mRNA. Translation: CAB38863.1.
U92655 mRNA. Translation: AAB51395.1. Frameshift.
RefSeqiNP_114462.1. NM_032073.1.
UniGeneiRn.9779.

3D structure databases

ProteinModelPortaliQ9Z0N7.
SMRiQ9Z0N7. Positions 584-620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249886. 1 interaction.
STRINGi10116.ENSRNOP00000027875.

Proteomic databases

PaxDbiQ9Z0N7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84020.
KEGGirno:84020.
UCSCiRGD:621503. rat.

Organism-specific databases

CTDi3784.
RGDi621503. Kcnq1.

Phylogenomic databases

eggNOGiKOG1419. Eukaryota.
COG1226. LUCA.
HOGENOMiHOG000220839.
HOVERGENiHBG059014.
InParanoidiQ9Z0N7.
KOiK04926.
PhylomeDBiQ9Z0N7.

Miscellaneous databases

PROiQ9Z0N7.

Gene expression databases

BgeeiENSRNOG00000020532.

Family and domain databases

InterProiIPR005821. Ion_trans_dom.
IPR003937. K_chnl_volt-dep_KCNQ.
IPR013821. K_chnl_volt-dep_KCNQ_C.
IPR005827. K_chnl_volt-dep_KCQN1.
IPR028325. VG_K_chnl.
[Graphical view]
PANTHERiPTHR11537. PTHR11537. 2 hits.
PTHR11537:SF151. PTHR11537:SF151. 2 hits.
PfamiPF00520. Ion_trans. 1 hit.
PF03520. KCNQ_channel. 1 hit.
[Graphical view]
PRINTSiPR00169. KCHANNEL.
PR01460. KCNQ1CHANNEL.
PR01459. KCNQCHANNEL.
ProtoNetiSearch...

Entry informationi

Entry nameiKCNQ1_RAT
AccessioniPrimary (citable) accession number: Q9Z0N7
Secondary accession number(s): O08655
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 1999
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.