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Protein

Eukaryotic translation initiation factor 2 subunit 3, X-linked

Gene

Eif2s3x

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage (PubMed:26823431).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi48 – 558GTPBy similarity
Nucleotide bindingi134 – 1385GTPBy similarity
Nucleotide bindingi190 – 1934GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-MMU-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit 3, X-linked
Alternative name(s):
Eukaryotic translation initiation factor 2 subunit gamma, X-linked
Short name:
eIF-2-gamma X
Gene namesi
Name:Eif2s3x
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1349431. Eif2s3x.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 472471Eukaryotic translation initiation factor 2 subunit 3, X-linkedPRO_0000137439Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei16 – 161PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z0N1.
MaxQBiQ9Z0N1.
PaxDbiQ9Z0N1.
PRIDEiQ9Z0N1.

PTM databases

iPTMnetiQ9Z0N1.
PhosphoSiteiQ9Z0N1.
SwissPalmiQ9Z0N1.

Expressioni

Tissue specificityi

Widely expressed. In the brain, high mRNA levels are observed in specific regions, including the habenula, anterodorsal thalamic nucleus, hippocampus, hypothalamus, and cerebellum. Also expressed in the embryonic brain. There is a differential expression between males and females, wich is tissue-specific. Females tend to have higher expression levels than males in the brain (cortex, hippocampus and paraventricular nucleus, but not in the habenula), as well as in other tissues. The up-regulation observed in females at the mRNA level may be due to the presence of 2 active copies of the gene.3 Publications

Gene expression databases

BgeeiQ9Z0N1.
ExpressionAtlasiQ9Z0N1. baseline and differential.
GenevisibleiQ9Z0N1. MM.

Interactioni

Subunit structurei

Heterotrimer composed of an alpha subunit, also called subunit 1 (encoded by EIF2S1), a beta subunit, also called subunit 2 (encoded by EIF2S2) and a gamma subunit, also called subunit 3 (encoded by 2 homologous genes Eif2s3x and Eif2s3y).Curated

Protein-protein interaction databases

BioGridi205054. 2 interactions.
IntActiQ9Z0N1. 3 interactions.
MINTiMINT-1855999.
STRINGi10090.ENSMUSP00000059395.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0N1.
SMRiQ9Z0N1. Positions 39-461.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 248210tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni48 – 558G1PROSITE-ProRule annotation
Regioni76 – 805G2PROSITE-ProRule annotation
Regioni134 – 1374G3PROSITE-ProRule annotation
Regioni190 – 1934G4PROSITE-ProRule annotation
Regioni225 – 2273G5PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0466. Eukaryota.
COG5257. LUCA.
GeneTreeiENSGT00550000074801.
HOGENOMiHOG000229292.
HOVERGENiHBG006123.
InParanoidiQ9Z0N1.
KOiK03242.
OMAiIAANEPC.
OrthoDBiEOG7H1JK8.
PhylomeDBiQ9Z0N1.
TreeFamiTF101513.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR015256. TIF2_gsu_C.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PfamiPF09173. eIF2_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0N1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGEGGVTL GQPHLSRQDL ATLDVTKLTP LSHEVISRQA TINIGTIGHV
60 70 80 90 100
AHGKSTVVKA ISGVHTVRFK NELERNITIK LGYANAKIYK LDDPSCPRPE
110 120 130 140 150
CYRSCGSSTP DEFPTDIPGT KGNFKLVRHV SFVDCPGHDI LMATMLNGAA
160 170 180 190 200
VMDAALLLIA GNESCPQPQT SEHLAAIEIM KLKHILILQN KIDLVKESQA
210 220 230 240 250
KEQYEQILAF VQGTVAEGAP IIPISAQLKY NIEVVCEYIV KKIPVPPRDF
260 270 280 290 300
TSEPRLIVIR SFDVNKPGCE VDDLKGGVAG GSILKGVLKV GQEIEVRPGI
310 320 330 340 350
VSKDSEGKLM CKPIFSKIVS LFAEHNDLQY AAPGGLIGVG TKIDPTLCRA
360 370 380 390 400
DRMVGQVLGA VGALPEIFTE LEISYFLLRR LLGVRTEGDK KAAKVQKLSK
410 420 430 440 450
NEVLMVNIGS LSTGGRVSAV KADLGKIVLT NPVCTEVGEK IALSRRVEKH
460 470
WRLIGWGQIR RGVTIKPTVD DD
Length:472
Mass (Da):51,065
Last modified:January 23, 2007 - v2
Checksum:i63CA694C06ED4CB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006587 mRNA. Translation: CAA07099.1.
BC063755 mRNA. Translation: AAH63755.1.
CCDSiCCDS30277.1.
RefSeqiNP_036140.1. NM_012010.3.
UniGeneiMm.218851.

Genome annotation databases

EnsembliENSMUST00000050328; ENSMUSP00000059395; ENSMUSG00000035150.
GeneIDi26905.
KEGGimmu:26905.
UCSCiuc009ttc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006587 mRNA. Translation: CAA07099.1.
BC063755 mRNA. Translation: AAH63755.1.
CCDSiCCDS30277.1.
RefSeqiNP_036140.1. NM_012010.3.
UniGeneiMm.218851.

3D structure databases

ProteinModelPortaliQ9Z0N1.
SMRiQ9Z0N1. Positions 39-461.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205054. 2 interactions.
IntActiQ9Z0N1. 3 interactions.
MINTiMINT-1855999.
STRINGi10090.ENSMUSP00000059395.

PTM databases

iPTMnetiQ9Z0N1.
PhosphoSiteiQ9Z0N1.
SwissPalmiQ9Z0N1.

Proteomic databases

EPDiQ9Z0N1.
MaxQBiQ9Z0N1.
PaxDbiQ9Z0N1.
PRIDEiQ9Z0N1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000050328; ENSMUSP00000059395; ENSMUSG00000035150.
GeneIDi26905.
KEGGimmu:26905.
UCSCiuc009ttc.1. mouse.

Organism-specific databases

CTDi26905.
MGIiMGI:1349431. Eif2s3x.

Phylogenomic databases

eggNOGiKOG0466. Eukaryota.
COG5257. LUCA.
GeneTreeiENSGT00550000074801.
HOGENOMiHOG000229292.
HOVERGENiHBG006123.
InParanoidiQ9Z0N1.
KOiK03242.
OMAiIAANEPC.
OrthoDBiEOG7H1JK8.
PhylomeDBiQ9Z0N1.
TreeFamiTF101513.

Enzyme and pathway databases

ReactomeiR-MMU-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-72649. Translation initiation complex formation.
R-MMU-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-MMU-72702. Ribosomal scanning and start codon recognition.
R-MMU-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-MMU-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiQ9Z0N1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z0N1.
ExpressionAtlasiQ9Z0N1. baseline and differential.
GenevisibleiQ9Z0N1. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR015256. TIF2_gsu_C.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PfamiPF09173. eIF2_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of genes encoding translation initiation factor eIF-2gamma in mouse and human: sex chromosome localization, escape from X-inactivation and evolution."
    Ehrmann I.E., Ellis P.S., Mazeyrat S., Duthie S., Brockdorff N., Mattei M.-G., Gavin M.A., Affara N.A., Brown G.M., Simpson E., Mitchell M.J., Scott D.M.
    Hum. Mol. Genet. 7:1725-1737(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, ESCAPE FROM X-INACTIVATION.
    Strain: C57BL/6 X CBA.
    Tissue: Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Limb.
  3. "Sex differences in sex chromosome gene expression in mouse brain."
    Xu J., Burgoyne P.S., Arnold A.P.
    Hum. Mol. Genet. 11:1409-1419(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Sexually dimorphic expression of the X-linked gene Eif2s3x mRNA but not protein in mouse brain."
    Xu J., Watkins R., Arnold A.P.
    Gene Expr. Patterns 6:146-155(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-16, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Liver, Pancreas, Spleen and Testis.
  7. "Two genes substitute for the mouse Y chromosome for spermatogenesis and reproduction."
    Yamauchi Y., Riel J.M., Ruthig V.A., Ortega E.A., Mitchell M.J., Ward M.A.
    Science 351:514-516(2016) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiIF2G_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Encoded by an chromosome X-linked gene which escapes inactivation. Has a homolog on chromosome Y (Eif2s3y).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.