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Protein

Eukaryotic translation initiation factor 2 subunit 3, X-linked

Gene

Eif2s3x

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage (PubMed:26823431).By similarity1 Publication

Miscellaneous

Encoded by an chromosome X-linked gene which escapes inactivation. Has a homolog on chromosome Y (Eif2s3y).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 55GTPBy similarity8
Nucleotide bindingi134 – 138GTPBy similarity5
Nucleotide bindingi190 – 193GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-MMU-382556 ABC-family proteins mediated transport
R-MMU-72649 Translation initiation complex formation
R-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition
R-MMU-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit 3, X-linked
Alternative name(s):
Eukaryotic translation initiation factor 2 subunit gamma, X-linked
Short name:
eIF-2-gamma X
Gene namesi
Name:Eif2s3x
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1349431 Eif2s3x

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001374392 – 472Eukaryotic translation initiation factor 2 subunit 3, X-linkedAdd BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei16PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z0N1
MaxQBiQ9Z0N1
PaxDbiQ9Z0N1
PRIDEiQ9Z0N1

PTM databases

iPTMnetiQ9Z0N1
PhosphoSitePlusiQ9Z0N1
SwissPalmiQ9Z0N1

Expressioni

Tissue specificityi

Widely expressed. In the brain, high mRNA levels are observed in specific regions, including the habenula, anterodorsal thalamic nucleus, hippocampus, hypothalamus, and cerebellum. Also expressed in the embryonic brain. There is a differential expression between males and females, wich is tissue-specific. Females tend to have higher expression levels than males in the brain (cortex, hippocampus and paraventricular nucleus, but not in the habenula), as well as in other tissues. The up-regulation observed in females at the mRNA level may be due to the presence of 2 active copies of the gene.3 Publications

Gene expression databases

BgeeiENSMUSG00000035150
ExpressionAtlasiQ9Z0N1 baseline and differential
GenevisibleiQ9Z0N1 MM

Interactioni

Subunit structurei

The eukaryotic translation initiation factor 2 complex/eIF2 is a heterotrimer composed of an alpha subunit, also called subunit 1 (encoded by EIF2S1), a beta subunit, also called subunit 2 (encoded by EIF2S2) and a gamma subunit, also called subunit 3 (encoded by 2 homologous genes Eif2s3x and Eif2s3y).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205054, 2 interactors
IntActiQ9Z0N1, 3 interactors
MINTiQ9Z0N1
STRINGi10090.ENSMUSP00000059395

Structurei

3D structure databases

ProteinModelPortaliQ9Z0N1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 248tr-type GPROSITE-ProRule annotationAdd BLAST210

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 55G1PROSITE-ProRule annotation8
Regioni76 – 80G2PROSITE-ProRule annotation5
Regioni134 – 137G3PROSITE-ProRule annotation4
Regioni190 – 193G4PROSITE-ProRule annotation4
Regioni225 – 227G5PROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0466 Eukaryota
COG5257 LUCA
GeneTreeiENSGT00550000074801
HOGENOMiHOG000229292
HOVERGENiHBG006123
InParanoidiQ9Z0N1
KOiK03242
OMAiCPQPQTK
OrthoDBiEOG091G0624
PhylomeDBiQ9Z0N1
TreeFamiTF101513

Family and domain databases

CDDicd15490 eIF2_gamma_III, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR015256 TIF2_gsu_C
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
PfamiView protein in Pfam
PF09173 eIF2_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0N1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGEGGVTL GQPHLSRQDL ATLDVTKLTP LSHEVISRQA TINIGTIGHV
60 70 80 90 100
AHGKSTVVKA ISGVHTVRFK NELERNITIK LGYANAKIYK LDDPSCPRPE
110 120 130 140 150
CYRSCGSSTP DEFPTDIPGT KGNFKLVRHV SFVDCPGHDI LMATMLNGAA
160 170 180 190 200
VMDAALLLIA GNESCPQPQT SEHLAAIEIM KLKHILILQN KIDLVKESQA
210 220 230 240 250
KEQYEQILAF VQGTVAEGAP IIPISAQLKY NIEVVCEYIV KKIPVPPRDF
260 270 280 290 300
TSEPRLIVIR SFDVNKPGCE VDDLKGGVAG GSILKGVLKV GQEIEVRPGI
310 320 330 340 350
VSKDSEGKLM CKPIFSKIVS LFAEHNDLQY AAPGGLIGVG TKIDPTLCRA
360 370 380 390 400
DRMVGQVLGA VGALPEIFTE LEISYFLLRR LLGVRTEGDK KAAKVQKLSK
410 420 430 440 450
NEVLMVNIGS LSTGGRVSAV KADLGKIVLT NPVCTEVGEK IALSRRVEKH
460 470
WRLIGWGQIR RGVTIKPTVD DD
Length:472
Mass (Da):51,065
Last modified:January 23, 2007 - v2
Checksum:i63CA694C06ED4CB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006587 mRNA Translation: CAA07099.1
BC063755 mRNA Translation: AAH63755.1
CCDSiCCDS30277.1
RefSeqiNP_036140.1, NM_012010.3
UniGeneiMm.218851

Genome annotation databases

EnsembliENSMUST00000050328; ENSMUSP00000059395; ENSMUSG00000035150
GeneIDi26905
KEGGimmu:26905
UCSCiuc009ttc.1 mouse

Similar proteinsi

Entry informationi

Entry nameiIF2G_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0N1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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