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Q9Z0M6 (CD97_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CD97 antigen
Alternative name(s):
CD_antigen=CD97
Gene names
Name:Cd97
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length818 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Ref.5

Subunit structure

Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit). Interacts with complement decay-accelerating factor (DAF). The largest isoform (isoform1) do not interact with DAF. Interacts also with chondroitin sulfate By similarity. Ref.2

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.2.

Tissue specificity

Although predominantly expressed by cells of the immune system is expressed ubiquitously, with particularly high levels of expression in the lung and the thymus gland. In the spleen, expression is detected on most myeloid cells and variable portions of T-cells, B-cells and NK cells. In the bone marrow, expressed in nearly all myeloid cells, whereas little if any expression is found on erythroid cells.

Induction

Up-regulated during lymphocyte activation.

Domain

The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region By similarity.

Binding to chondroitin sulfate is mediated by the fourth EGF domain By similarity.

Post-translational modification

Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.

Contains 4 EGF-like domains.

Contains 1 GPS domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Z0M6-1)

Also known as: EGF(1,2,X,3,4);

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Z0M6-2)

Also known as: EGF(1,2,4);

The sequence of this isoform differs from the canonical sequence as follows:
     120-213: Missing.
Isoform 3 (identifier: Q9Z0M6-3)

Also known as: EGF(1,2,3,4);

The sequence of this isoform differs from the canonical sequence as follows:
     120-164: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 818795CD97 antigen
PRO_0000012869

Regions

Topological domain24 – 533510Extracellular Potential
Transmembrane534 – 55421Helical; Name=1; Potential
Topological domain555 – 5628Cytoplasmic Potential
Transmembrane563 – 58321Helical; Name=2; Potential
Topological domain584 – 60219Extracellular Potential
Transmembrane603 – 62321Helical; Name=3; Potential
Topological domain624 – 63714Cytoplasmic Potential
Transmembrane638 – 65821Helical; Name=4; Potential
Topological domain659 – 67921Extracellular Potential
Transmembrane680 – 70021Helical; Name=5; Potential
Topological domain701 – 72323Cytoplasmic Potential
Transmembrane724 – 74421Helical; Name=6; Potential
Topological domain745 – 7528Extracellular Potential
Transmembrane753 – 77321Helical; Name=7; Potential
Topological domain774 – 81845Cytoplasmic Potential
Domain27 – 6842EGF-like 1
Domain69 – 11951EGF-like 2; calcium-binding Potential
Domain165 – 21349EGF-like 3; calcium-binding Potential
Domain214 – 26148EGF-like 4; calcium-binding Potential
Domain479 – 52446GPS

Sites

Site513 – 5142Cleavage By similarity

Amino acid modifications

Modified residue8141Phosphoserine By similarity
Modified residue8161Phosphoserine By similarity
Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation1121N-linked (GlcNAc...) Potential
Glycosylation2271N-linked (GlcNAc...) Potential
Glycosylation2991N-linked (GlcNAc...) Ref.6 Ref.7
Glycosylation3951N-linked (GlcNAc...) Ref.6 Ref.7
Glycosylation4071N-linked (GlcNAc...); atypical Ref.6 Ref.7
Glycosylation4611N-linked (GlcNAc...) Ref.6
Glycosylation5021N-linked (GlcNAc...) Potential
Disulfide bond31 ↔ 41 By similarity
Disulfide bond35 ↔ 47 By similarity
Disulfide bond49 ↔ 67 By similarity
Disulfide bond73 ↔ 86 By similarity
Disulfide bond80 ↔ 95 By similarity
Disulfide bond97 ↔ 118 By similarity
Disulfide bond169 ↔ 182 By similarity
Disulfide bond176 ↔ 191 By similarity
Disulfide bond193 ↔ 212 By similarity
Disulfide bond218 ↔ 231 By similarity
Disulfide bond225 ↔ 240 By similarity
Disulfide bond242 ↔ 260 By similarity

Natural variations

Alternative sequence120 – 21394Missing in isoform 2.
VSP_009413
Alternative sequence120 – 16445Missing in isoform 3.
VSP_009414

Experimental info

Sequence conflict31G → S in CAB38246. Ref.1
Sequence conflict281S → I in CAB38246. Ref.1
Sequence conflict3201Q → E in AAH06676. Ref.3
Sequence conflict5061F → S in AAF67800. Ref.2
Sequence conflict5991M → V in AAH06676. Ref.3
Sequence conflict7261A → S Ref.3
Sequence conflict7261A → S Ref.4
Sequence conflict7961F → I in BAB25461. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (EGF(1,2,X,3,4)) [UniParc].

Last modified February 16, 2004. Version 2.
Checksum: E59A292F2CF626C2

FASTA81890,413
        10         20         30         40         50         60 
MRGVRCPGLL VVCILLSLSG AGTQKAESKN CAKWCPINSK CVSNRSCVCK PGFSSEKELI 

        70         80         90        100        110        120 
TNPAESCEDI NECLLPGFSC GDFAMCKNSE GSYTCVCNLG YKLLSGAESF VNESENTCQA 

       130        140        150        160        170        180 
SVNTGTTPVP SRIHTVTTAP GNLPEQTTTV HQTQMGDSEE RTPKDVNECI SGQNHCHQST 

       190        200        210        220        230        240 
HCINKLGGYS CICRQGWKPV PGSPNGPVST VCEDVDECSS GQHQCHNSTV CKNTVGSYKC 

       250        260        270        280        290        300 
HCRPGWKPTS GSLRGPDTIC QEPPFPTWTL LPTAHSQTLL RFSVEVQNLL RDFNPATVNY 

       310        320        330        340        350        360 
TIQKLIEAVD KLLEDPMETQ TQQVAAQLLS NLEQSLRTLA QFLPKGPFTY TSPSNTELSL 

       370        380        390        400        410        420 
MVKEQDNKDV TTVHHGQTWM ELDWAVTAGA KISENGSSVA GILSSPNMEK LLGNTPLNLE 

       430        440        450        460        470        480 
QRRASLEDFY GSPIPSVSLK LLSNINSVFL TNTNTEKLAS NVTFKFDFTS VESIEPRHEL 

       490        500        510        520        530        540 
ICAFWKAHNG NGYWDTDGCS MNGTGFCHCN HLTSFAILMA QYHVQDPRLE LITKVGLLLS 

       550        560        570        580        590        600 
LICLLLCILT FLLVKPIQSS RTMVHLHLCI CLFLGSIIFL VGVENEGGEV GLRCRLVAMM 

       610        620        630        640        650        660 
LHFCFLAAFC WMALEGVELY FLVVRVFQGQ GLSTWQRCLI GYGVPLLIVA ISMAVVKMDG 

       670        680        690        700        710        720 
YGHATYCWLD FRKQGFLWSF SGPVAFIIFC NAAIFVITVW KLTKKFSEIN PNMKKLRKAR 

       730        740        750        760        770        780 
VLTITAIAQL LVLGCTWGFG LFLFNPHSTW LSYIFTLLNC LQGLFLYVML CLLNKKVREE 

       790        800        810 
YWKWACMVTG SKYTEFNSST TGTGTSQTRA LRSSESGM 

« Hide

Isoform 2 (EGF(1,2,4)) [UniParc].

Checksum: 7DA1962863ED5A5F
Show »

FASTA72480,433
Isoform 3 (EGF(1,2,3,4)) [UniParc].

Checksum: E18D892177ECC3F9
Show »

FASTA77385,657

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of mouse CD97."
Hamann J., van Zventer C., Bijl A., Molenaar C., Tesselaar K., van Lier R.A.W.
Int. Immunol. 12:439-448(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
[2]"Structural characterization of mouse CD97 and study of its specific interaction with the murine decay-accelerating factor (DAF, CD55)."
Qian Y.-M., Haino M., Kelly K., Song W.-C.
Immunology 98:303-311(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3), INTERACTION WITH DAF, SUBCELLULAR LOCATION.
Tissue: Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 607-818.
Strain: C57BL/6J.
Tissue: Small intestine.
[5]"The epidermal growth factor-seven transmembrane (EGF-TM7) receptor CD97 is required for neutrophil migration and host defense."
Leemans J.C., te Velde A.A., Florquin S., Bennink R.J., de Bruin K., van Lier R.A.W., van der Poll T., Hamann J.
J. Immunol. 172:1125-1131(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-299; ASN-395; ASN-407 AND ASN-461.
Tissue: Myoblast.
[7]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-299; ASN-395 AND ASN-407.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y18365 mRNA. Translation: CAB38246.1.
AF146344 mRNA. Translation: AAF67800.1.
BC006676 mRNA. Translation: AAH06676.1.
AK008101 mRNA. Translation: BAB25461.1.
RefSeqNP_001156501.1. NM_001163029.1.
UniGeneMm.334648.

3D structure databases

ProteinModelPortalQ9Z0M6.
SMRQ9Z0M6. Positions 29-261, 518-793.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9Z0M6. 1 interaction.
MINTMINT-4090290.
STRING10090.ENSMUSP00000075240.

Protein family/group databases

MEROPSS63.036.
GPCRDBSearch...

PTM databases

PhosphoSiteQ9Z0M6.

Proteomic databases

PaxDbQ9Z0M6.
PRIDEQ9Z0M6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002964; ENSMUSP00000002964; ENSMUSG00000002885.
GeneID26364.
KEGGmmu:26364.

Organism-specific databases

CTD976.
MGIMGI:1347095. Cd97.

Phylogenomic databases

eggNOGNOG320737.
GeneTreeENSGT00710000106562.
HOGENOMHOG000294115.
HOVERGENHBG048917.
InParanoidQ9Z0M6.
KOK08446.
OrthoDBEOG75J0MK.
PhylomeDBQ9Z0M6.

Gene expression databases

ArrayExpressQ9Z0M6.
BgeeQ9Z0M6.
CleanExMM_CD97.
GenevestigatorQ9Z0M6.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR003056. GPCR_2_CD97.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 3 hits.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSPR01278. CD97PROTEIN.
PR00249. GPCRSECRETIN.
SMARTSM00181. EGF. 1 hit.
SM00179. EGF_CA. 3 hits.
SM00303. GPS. 1 hit.
[Graphical view]
PROSITEPS00010. ASX_HYDROXYL. 3 hits.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 3 hits.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCD97. mouse.
NextBio304231.
PROQ9Z0M6.
SOURCESearch...

Entry information

Entry nameCD97_MOUSE
AccessionPrimary (citable) accession number: Q9Z0M6
Secondary accession number(s): Q923A1, Q9CVI5, Q9JLQ8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: April 16, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries