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Protein

CD97 antigen

Gene

Cd97

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processCell adhesion
LigandCalcium

Protein family/group databases

MEROPSiP02.033.

Names & Taxonomyi

Protein namesi
Recommended name:
CD97 antigen
Alternative name(s):
CD_antigen: CD97
Cleaved into the following 2 chains:
Gene namesi
Name:Cd97
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1347095. Cd97.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 533ExtracellularSequence analysisAdd BLAST510
Transmembranei534 – 554Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini555 – 562CytoplasmicSequence analysis8
Transmembranei563 – 583Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini584 – 602ExtracellularSequence analysisAdd BLAST19
Transmembranei603 – 623Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini624 – 637CytoplasmicSequence analysisAdd BLAST14
Transmembranei638 – 658Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini659 – 679ExtracellularSequence analysisAdd BLAST21
Transmembranei680 – 700Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini701 – 723CytoplasmicSequence analysisAdd BLAST23
Transmembranei724 – 744Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini745 – 752ExtracellularSequence analysis8
Transmembranei753 – 773Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini774 – 818CytoplasmicSequence analysisAdd BLAST45

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001286924 – 818CD97 antigenAdd BLAST795
ChainiPRO_000043512624 – 513CD97 antigen subunit alphaBy similarityAdd BLAST490
ChainiPRO_0000435127514 – 818CD97 antigen subunit betaBy similarityAdd BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 41PROSITE-ProRule annotation
Disulfide bondi35 ↔ 47PROSITE-ProRule annotation
Glycosylationi44N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi49 ↔ 67PROSITE-ProRule annotation
Disulfide bondi73 ↔ 86PROSITE-ProRule annotation
Disulfide bondi80 ↔ 95PROSITE-ProRule annotation
Disulfide bondi97 ↔ 118PROSITE-ProRule annotation
Glycosylationi112N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi169 ↔ 182PROSITE-ProRule annotation
Disulfide bondi176 ↔ 191PROSITE-ProRule annotation
Disulfide bondi193 ↔ 212PROSITE-ProRule annotation
Disulfide bondi218 ↔ 231PROSITE-ProRule annotation
Disulfide bondi225 ↔ 240PROSITE-ProRule annotation
Glycosylationi227N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi242 ↔ 260PROSITE-ProRule annotation
Glycosylationi299N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi395N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi407N-linked (GlcNAc...) asparagine; atypical2 Publications1
Modified residuei425PhosphoserineCombined sources1
Glycosylationi461N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi502N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei798PhosphoserineBy similarity1
Modified residuei808PhosphothreonineBy similarity1
Modified residuei814PhosphoserineCombined sources1
Modified residuei816PhosphoserineBy similarity1

Post-translational modificationi

Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei513 – 514CleavageBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9Z0M6.
PRIDEiQ9Z0M6.

PTM databases

iPTMnetiQ9Z0M6.
PhosphoSitePlusiQ9Z0M6.

Expressioni

Tissue specificityi

Although predominantly expressed by cells of the immune system is expressed ubiquitously, with particularly high levels of expression in the lung and the thymus gland. In the spleen, expression is detected on most myeloid cells and variable portions of T-cells, B-cells and NK cells. In the bone marrow, expressed in nearly all myeloid cells, whereas little if any expression is found on erythroid cells.

Inductioni

Up-regulated during lymphocyte activation.

Gene expression databases

CleanExiMM_CD97.

Interactioni

Subunit structurei

Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit). Interacts with complement decay-accelerating factor (DAF). The largest isoform (isoform 1) do not interact with DAF. Interacts also with chondroitin sulfate (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9Z0M6. 1 interactor.
MINTiMINT-4090290.
STRINGi10090.ENSMUSP00000075240.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0M6.
SMRiQ9Z0M6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 68EGF-like 1PROSITE-ProRule annotationAdd BLAST42
Domaini69 – 119EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd BLAST51
Domaini165 – 213EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST49
Domaini214 – 261EGF-like 4; calcium-bindingPROSITE-ProRule annotationAdd BLAST48
Domaini479 – 524GPSPROSITE-ProRule annotationAdd BLAST46

Domaini

The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region (By similarity).By similarity
Binding to chondroitin sulfate is mediated by the fourth EGF domain.By similarity

Sequence similaritiesi

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ9Z0M6.
KOiK08446.
PhylomeDBiQ9Z0M6.

Family and domain databases

Gene3Di2.40.155.10. 2 hits.
InterProiView protein in InterPro
IPR001881. EGF-like_Ca-bd_dom.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009017. GFP.
IPR017981. GPCR_2-like.
IPR003056. GPCR_2_CD97.
IPR000832. GPCR_2_secretin-like.
IPR000203. GPS.
PfamiView protein in Pfam
PF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 3 hits.
PF01825. GPS. 1 hit.
PRINTSiPR01278. CD97PROTEIN.
PR00249. GPCRSECRETIN.
SMARTiView protein in SMART
SM00181. EGF. 4 hits.
SM00179. EGF_CA. 3 hits.
SM00303. GPS. 1 hit.
PROSITEiView protein in PROSITE
PS00010. ASX_HYDROXYL. 3 hits.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 3 hits.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z0M6-1) [UniParc]FASTAAdd to basket
Also known as: EGF(1,2,X,3,4)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGVRCPGLL VVCILLSLSG AGTQKAESKN CAKWCPINSK CVSNRSCVCK
60 70 80 90 100
PGFSSEKELI TNPAESCEDI NECLLPGFSC GDFAMCKNSE GSYTCVCNLG
110 120 130 140 150
YKLLSGAESF VNESENTCQA SVNTGTTPVP SRIHTVTTAP GNLPEQTTTV
160 170 180 190 200
HQTQMGDSEE RTPKDVNECI SGQNHCHQST HCINKLGGYS CICRQGWKPV
210 220 230 240 250
PGSPNGPVST VCEDVDECSS GQHQCHNSTV CKNTVGSYKC HCRPGWKPTS
260 270 280 290 300
GSLRGPDTIC QEPPFPTWTL LPTAHSQTLL RFSVEVQNLL RDFNPATVNY
310 320 330 340 350
TIQKLIEAVD KLLEDPMETQ TQQVAAQLLS NLEQSLRTLA QFLPKGPFTY
360 370 380 390 400
TSPSNTELSL MVKEQDNKDV TTVHHGQTWM ELDWAVTAGA KISENGSSVA
410 420 430 440 450
GILSSPNMEK LLGNTPLNLE QRRASLEDFY GSPIPSVSLK LLSNINSVFL
460 470 480 490 500
TNTNTEKLAS NVTFKFDFTS VESIEPRHEL ICAFWKAHNG NGYWDTDGCS
510 520 530 540 550
MNGTGFCHCN HLTSFAILMA QYHVQDPRLE LITKVGLLLS LICLLLCILT
560 570 580 590 600
FLLVKPIQSS RTMVHLHLCI CLFLGSIIFL VGVENEGGEV GLRCRLVAMM
610 620 630 640 650
LHFCFLAAFC WMALEGVELY FLVVRVFQGQ GLSTWQRCLI GYGVPLLIVA
660 670 680 690 700
ISMAVVKMDG YGHATYCWLD FRKQGFLWSF SGPVAFIIFC NAAIFVITVW
710 720 730 740 750
KLTKKFSEIN PNMKKLRKAR VLTITAIAQL LVLGCTWGFG LFLFNPHSTW
760 770 780 790 800
LSYIFTLLNC LQGLFLYVML CLLNKKVREE YWKWACMVTG SKYTEFNSST
810
TGTGTSQTRA LRSSESGM
Length:818
Mass (Da):90,413
Last modified:February 16, 2004 - v2
Checksum:iE59A292F2CF626C2
GO
Isoform 2 (identifier: Q9Z0M6-2) [UniParc]FASTAAdd to basket
Also known as: EGF(1,2,4)

The sequence of this isoform differs from the canonical sequence as follows:
     120-213: Missing.

Show »
Length:724
Mass (Da):80,433
Checksum:i7DA1962863ED5A5F
GO
Isoform 3 (identifier: Q9Z0M6-3) [UniParc]FASTAAdd to basket
Also known as: EGF(1,2,3,4)

The sequence of this isoform differs from the canonical sequence as follows:
     120-164: Missing.

Show »
Length:773
Mass (Da):85,657
Checksum:iE18D892177ECC3F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3G → S in CAB38246 (PubMed:10744645).Curated1
Sequence conflicti28S → I in CAB38246 (PubMed:10744645).Curated1
Sequence conflicti320Q → E in AAH06676 (PubMed:15489334).Curated1
Sequence conflicti506F → S in AAF67800 (PubMed:10540231).Curated1
Sequence conflicti599M → V in AAH06676 (PubMed:15489334).Curated1
Sequence conflicti726A → S (PubMed:15489334).Curated1
Sequence conflicti726A → S (PubMed:16141072).Curated1
Sequence conflicti796F → I in BAB25461 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009413120 – 213Missing in isoform 2. 3 PublicationsAdd BLAST94
Alternative sequenceiVSP_009414120 – 164Missing in isoform 3. 2 PublicationsAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y18365 mRNA. Translation: CAB38246.1.
AF146344 mRNA. Translation: AAF67800.1.
BC006676 mRNA. Translation: AAH06676.1.
AK008101 mRNA. Translation: BAB25461.1.
CCDSiCCDS22461.1. [Q9Z0M6-1]
CCDS52610.1. [Q9Z0M6-2]
CCDS52611.1. [Q9Z0M6-3]
RefSeqiNP_001156501.1. NM_001163029.1.
UniGeneiMm.334648.

Genome annotation databases

GeneIDi26364.
KEGGimmu:26364.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCD97_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0M6
Secondary accession number(s): Q923A1, Q9CVI5, Q9JLQ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: February 16, 2004
Last modified: October 25, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families