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Protein

Lysosomal acid lipase/cholesteryl ester hydrolase

Gene

Lipa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation (By similarity).By similarity

Catalytic activityi

A steryl ester + H2O = a sterol + a fatty acid.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei172Charge relay systemPROSITE-ProRule annotation1
Active sitei372Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cell morphogenesis Source: MGI
  • cell proliferation Source: MGI
  • cytokine production Source: MGI
  • homeostasis of number of cells within a tissue Source: MGI
  • inflammatory response Source: MGI
  • lipid catabolic process Source: UniProtKB-KW
  • lung development Source: MGI
  • tissue remodeling Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-171052. LDL-mediated lipid transport.

Protein family/group databases

ESTHERimouse-1llip. Acidic_Lipase.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal acid lipase/cholesteryl ester hydrolase (EC:3.1.1.13)
Short name:
Acid cholesteryl ester hydrolase
Short name:
LAL
Alternative name(s):
Cholesteryl esterase
Lipase A
Sterol esterase
Gene namesi
Name:Lipa
Synonyms:Lip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:96789. Lipa.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000001780026 – 397Lysosomal acid lipase/cholesteryl ester hydrolaseAdd BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi34N-linked (GlcNAc...)Sequence analysis1
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Glycosylationi159N-linked (GlcNAc...)Sequence analysis1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi319N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9Z0M5.
MaxQBiQ9Z0M5.
PaxDbiQ9Z0M5.
PRIDEiQ9Z0M5.

PTM databases

iPTMnetiQ9Z0M5.
PhosphoSitePlusiQ9Z0M5.

Expressioni

Tissue specificityi

Expressed at low levels in most tissues. High level expression is found in hepatocytes and splenic and thymic cells. Very high level expression is observed in cells of the small intestinal villi, the zona fasciculata and reticularis of the adrenal cortex, pancreatic acini, and renal tubular epithelium.

Gene expression databases

BgeeiENSMUSG00000024781.
CleanExiMM_LIPA.
GenevisibleiQ9Z0M5. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9Z0M5. 2 interactors.
MINTiMINT-4114169.
STRINGi10090.ENSMUSP00000053270.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0M5.
SMRiQ9Z0M5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 378AB hydrolase-1Sequence analysisAdd BLAST301

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2624. Eukaryota.
COG0596. LUCA.
GeneTreeiENSGT00550000074328.
HOGENOMiHOG000240694.
HOVERGENiHBG006265.
InParanoidiQ9Z0M5.
KOiK01052.
OMAiVCTHVIM.
OrthoDBiEOG091G09AX.
TreeFamiTF315485.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR025483. Lipase_euk.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000862. Steryl_ester_lip. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0M5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLQGLVFVF TIGILLSRVP TGTVSAVDPE VNMNVTEIIM RWGYPGEEHS
60 70 80 90 100
VLTGDGYILS IHRIPRGRKN HFGKGPRPVV YLQHGLLADS SNWVTNIDNS
110 120 130 140 150
SLGFLLADAG FDVWMGNSRG NTWSLKHKTL SVSQDEFWAF SFDEMAKYDL
160 170 180 190 200
PASINYILNK TGQEQIYYVG HSQGCTIGFI AFSQMPELAK KIKMFLVLAP
210 220 230 240 250
VLSLNFASGP LLQLGRLPDP LLKDMFGQKQ FLPQSAMLKW LSIHVCTHVI
260 270 280 290 300
MKELCANVFF LLCGFNEKNL NMSRVDVYTT HCPAGTSVQN MLHWGQVFKY
310 320 330 340 350
RKLQAFDWGS SEKNYFHYNQ SFPPSYNIKN MRLPTALWSG GRDWLADIND
360 370 380 390
ITILLTQIPK LVYHKNIPEW DHLDFIWGLD APWKLYDEII SLMKKYQ
Length:397
Mass (Da):45,325
Last modified:July 27, 2011 - v2
Checksum:i866B123A1A058134
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68R → W in CAA83494 (PubMed:8725147).Curated1
Sequence conflicti109A → R in CAA83494 (PubMed:8725147).Curated1
Sequence conflicti285 – 287GTS → ELL in CAA83494 (PubMed:8725147).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z31689 mRNA. Translation: CAA83494.1.
AK088659 mRNA. Translation: BAC40484.1.
AK149791 mRNA. Translation: BAE29088.1.
AK164007 mRNA. Translation: BAE37585.1.
CH466534 Genomic DNA. Translation: EDL41750.1.
CCDSiCCDS29760.1.
RefSeqiNP_001104570.1. NM_001111100.1.
NP_067435.3. NM_021460.3.
UniGeneiMm.157545.

Genome annotation databases

EnsembliENSMUST00000049572; ENSMUSP00000053270; ENSMUSG00000024781.
ENSMUST00000178114; ENSMUSP00000136967; ENSMUSG00000024781.
GeneIDi16889.
KEGGimmu:16889.
UCSCiuc008hgm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z31689 mRNA. Translation: CAA83494.1.
AK088659 mRNA. Translation: BAC40484.1.
AK149791 mRNA. Translation: BAE29088.1.
AK164007 mRNA. Translation: BAE37585.1.
CH466534 Genomic DNA. Translation: EDL41750.1.
CCDSiCCDS29760.1.
RefSeqiNP_001104570.1. NM_001111100.1.
NP_067435.3. NM_021460.3.
UniGeneiMm.157545.

3D structure databases

ProteinModelPortaliQ9Z0M5.
SMRiQ9Z0M5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z0M5. 2 interactors.
MINTiMINT-4114169.
STRINGi10090.ENSMUSP00000053270.

Protein family/group databases

ESTHERimouse-1llip. Acidic_Lipase.

PTM databases

iPTMnetiQ9Z0M5.
PhosphoSitePlusiQ9Z0M5.

Proteomic databases

EPDiQ9Z0M5.
MaxQBiQ9Z0M5.
PaxDbiQ9Z0M5.
PRIDEiQ9Z0M5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049572; ENSMUSP00000053270; ENSMUSG00000024781.
ENSMUST00000178114; ENSMUSP00000136967; ENSMUSG00000024781.
GeneIDi16889.
KEGGimmu:16889.
UCSCiuc008hgm.2. mouse.

Organism-specific databases

CTDi3988.
MGIiMGI:96789. Lipa.

Phylogenomic databases

eggNOGiKOG2624. Eukaryota.
COG0596. LUCA.
GeneTreeiENSGT00550000074328.
HOGENOMiHOG000240694.
HOVERGENiHBG006265.
InParanoidiQ9Z0M5.
KOiK01052.
OMAiVCTHVIM.
OrthoDBiEOG091G09AX.
TreeFamiTF315485.

Enzyme and pathway databases

ReactomeiR-MMU-171052. LDL-mediated lipid transport.

Miscellaneous databases

ChiTaRSiLipa. mouse.
PROiQ9Z0M5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024781.
CleanExiMM_LIPA.
GenevisibleiQ9Z0M5. MM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR025483. Lipase_euk.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000862. Steryl_ester_lip. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLICH_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0M5
Secondary accession number(s): Q8C2G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.