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Protein

Kallikrein-4

Gene

Klk4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a major role in enamel formation. Required during the maturation stage of tooth development for clearance of enamel proteins and normal structural patterning of the crystalline matrix.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi41 – 411ZincBy similarity
Active sitei72 – 721Charge relay systemPROSITE-ProRule annotation
Metal bindingi92 – 921ZincBy similarity
Active sitei117 – 1171Charge relay systemPROSITE-ProRule annotation
Active sitei208 – 2081Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • amelogenesis Source: MGI
  • biomineral tissue development Source: UniProtKB-KW
  • extracellular matrix disassembly Source: MGI
  • protein catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Biomineralization

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.21.B12. 3474.

Protein family/group databases

MEROPSiS01.251.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-4By similarity (EC:3.4.21.-PROSITE-ProRule annotation)
Alternative name(s):
Enamel matrix serine proteinase 11 Publication
Kallikrein-like protein 1By similarity
Serine protease 171 Publication
Gene namesi
Name:Klk4Imported
Synonyms:EMSP11 Publication, KLK-L1Imported, Prss171 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1861379. Klk4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Viable and fertile, when reared on a soft diet. Tooth morphology is grossly normal but the enamel surface is fragile and rapidly abraded. Although formation of the enamel layer is initially normal, the crystallites fail to thicken and interlock. The enamel proteins enamelin and amelogenin are not cleared and persist in the matrix during the maturation stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Propeptidei26 – 316By similarityPRO_0000436024
Chaini32 – 255224Kallikrein-4Sequence analysisPRO_5006493980Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 73PROSITE-ProRule annotation
Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence analysis
Disulfide bondi149 ↔ 214PROSITE-ProRule annotation
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence analysis
Disulfide bondi179 ↔ 193PROSITE-ProRule annotation
Disulfide bondi204 ↔ 229PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated. The N-glycan structures are of complex diantennary or triantennary type, which may be further modified with up to 2 sialic acid residues.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9JIS2.
PRIDEiQ9JIS2.

PTM databases

PhosphoSiteiQ9Z0M1.

Expressioni

Developmental stagei

In developing teeth, expressed in ameloblasts during transition and maturation stages (PubMed:10863090, PubMed:10690663, PubMed:19578120). Expressed weakly in odontoblasts (PubMed:10863090). Not detected in odontoblasts (PubMed:19578120). Detected in the epithelium surrounding the erupted first molar (PubMed:10690663).3 Publications

Gene expression databases

BgeeiENSMUSG00000006948.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000007161.

Structurei

3D structure databases

SMRiQ9Z0M1. Positions 32-253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 253222Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
KOiK08666.
OMAiASEETCR.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ9Z0M1.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0M1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVTARTPWG WFLGCLILEV TGASASSVSS RIIQGQDCSP HSQPWQAALF
60 70 80 90 100
SEDGFFCSGV LVHPQWVLSA AHCLQESYIV GLGLHNLKGS QEPGSRMLEA
110 120 130 140 150
HLSIQHPNFN DPSFANDLML IKLNESVIES NTIRSIPVAT QCPTPGDTCL
160 170 180 190 200
VSGWGQLKNG KLPSLLQCVN LSVASEETCR LLYDPVYHLS MFCAGGGQDQ
210 220 230 240 250
KDSCNGDSGG PIVCNRSLQG LVSMGQGKCG QPGIPSVYTN LCKFTNWIQT

IIQTN
Length:255
Mass (Da):27,488
Last modified:May 1, 1999 - v1
Checksum:i6FD2E7DEA0660A2A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151C → Y in AAF85937 (PubMed:10863090).Curated
Sequence conflicti251 – 2511I → T in AAF85937 (PubMed:10863090).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198031 Genomic DNA. Translation: AAF85937.1.
AF019979 mRNA. Translation: AAC98894.1.
AC134858 Genomic DNA. No translation available.
CH466603 Genomic DNA. Translation: EDL22687.1.
BC100716 mRNA. Translation: AAI00717.1.
BC100717 mRNA. Translation: AAI00718.1.
BC100718 mRNA. Translation: AAI00719.1.
BC100719 mRNA. Translation: AAI00720.1.
CCDSiCCDS21186.1.
RefSeqiNP_064312.1. NM_019928.1.
UniGeneiMm.42080.

Genome annotation databases

EnsembliENSMUST00000007161; ENSMUSP00000007161; ENSMUSG00000006948.
GeneIDi56640.
KEGGimmu:56640.
UCSCiuc009gny.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198031 Genomic DNA. Translation: AAF85937.1.
AF019979 mRNA. Translation: AAC98894.1.
AC134858 Genomic DNA. No translation available.
CH466603 Genomic DNA. Translation: EDL22687.1.
BC100716 mRNA. Translation: AAI00717.1.
BC100717 mRNA. Translation: AAI00718.1.
BC100718 mRNA. Translation: AAI00719.1.
BC100719 mRNA. Translation: AAI00720.1.
CCDSiCCDS21186.1.
RefSeqiNP_064312.1. NM_019928.1.
UniGeneiMm.42080.

3D structure databases

SMRiQ9Z0M1. Positions 32-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000007161.

Protein family/group databases

MEROPSiS01.251.

PTM databases

PhosphoSiteiQ9Z0M1.

Proteomic databases

PaxDbiQ9JIS2.
PRIDEiQ9JIS2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007161; ENSMUSP00000007161; ENSMUSG00000006948.
GeneIDi56640.
KEGGimmu:56640.
UCSCiuc009gny.1. mouse.

Organism-specific databases

CTDi9622.
MGIiMGI:1861379. Klk4.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
KOiK08666.
OMAiASEETCR.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ9Z0M1.
TreeFamiTF331065.

Enzyme and pathway databases

BRENDAi3.4.21.B12. 3474.

Miscellaneous databases

SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006948.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKLK4_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0M1
Secondary accession number(s): Q9JIS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2016
Last sequence update: May 1, 1999
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.