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Protein

Trophoblast glycoprotein

Gene

Tpbg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.By similarity

GO - Biological processi

  • positive regulation of synapse assembly Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Trophoblast glycoprotein
Alternative name(s):
5T4 oncofetal trophoblast glycoprotein
Short name:
5T4 oncotrophoblast glycoprotein
Wnt-activated inhibitory factor 1
Short name:
WAIF1
Gene namesi
Name:Tpbg
Synonyms:5t4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1341264. Tpbg.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 361330ExtracellularSequence AnalysisAdd
BLAST
Transmembranei362 – 38221HelicalSequence AnalysisAdd
BLAST
Topological domaini383 – 42644CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: MGI
  • cytoplasm Source: MGI
  • endoplasmic reticulum Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 426395Trophoblast glycoproteinPRO_0000019593Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi62 ↔ 68By similarity
Disulfide bondi66 ↔ 77By similarity
Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi281 – 2811N-linked (GlcNAc...)1 Publication
Disulfide bondi304 ↔ 329By similarity
Disulfide bondi306 ↔ 350By similarity

Post-translational modificationi

Highly glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9Z0L0.
PaxDbiQ9Z0L0.
PRIDEiQ9Z0L0.

PTM databases

PhosphoSiteiQ9Z0L0.

Expressioni

Tissue specificityi

Highly expressed in embryo and placenta. In adult, expressed only in brain and ovary. Not detected in kidney small intestine, heart, spleen, testis, liver, lung, thymus and stomach.1 Publication

Gene expression databases

BgeeiQ9Z0L0.
CleanExiMM_TPBG.
ExpressionAtlasiQ9Z0L0. baseline and differential.
GenevestigatoriQ9Z0L0.

Structurei

3D structure databases

ProteinModelPortaliQ9Z0L0.
SMRiQ9Z0L0. Positions 59-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 9139LRRNTAdd
BLAST
Repeati92 – 11322LRR 1Add
BLAST
Repeati116 – 13924LRR 2Add
BLAST
Repeati141 – 16323LRR 3Add
BLAST
Repeati172 – 21039LRR 4Add
BLAST
Repeati215 – 23824LRR 5Add
BLAST
Repeati239 – 26123LRR 6Add
BLAST
Repeati262 – 28120LRR 7Add
BLAST
Domaini289 – 35264LRRCTAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi32 – 5322Ser-richAdd
BLAST

Domaini

The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway.By similarity

Sequence similaritiesi

Contains 7 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG301461.
GeneTreeiENSGT00620000088023.
HOGENOMiHOG000013090.
HOVERGENiHBG053843.
InParanoidiQ9Z0L0.
OMAiTYVSFRN.
OrthoDBiEOG7QNVM8.
TreeFamiTF351115.

Family and domain databases

InterProiIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF01462. LRRNT. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z0L0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGAGSRGPS AGDGRLRLAR LALVLLGWVS ASAPSSSVPS SSTSPAAFLA
60 70 80 90 100
SGSAQPPPAE RCPAACECSE AARTVKCVNR NLLEVPADLP PYVRNLFLTG
110 120 130 140 150
NQMTVLPAGA FARQPPLADL EALNLSGNHL KEVCAGAFEH LPGLRRLDLS
160 170 180 190 200
HNPLTNLSAF AFAGSNASVS APSPLEELIL NHIVPPEDQR QNGSFEGMVA
210 220 230 240 250
FEGMVAAALR SGLALRGLTR LELASNHFLF LPRDLLAQLP SLRYLDLRNN
260 270 280 290 300
SLVSLTYASF RNLTHLESLH LEDNALKVLH NSTLAEWHGL AHVKVFLDNN
310 320 330 340 350
PWVCDCYMAD MVAWLKETEV VPDKARLTCA FPEKMRNRGL LDLNSSDLDC
360 370 380 390 400
DAVLPQSLQT SYVFLGIVLA LIGAIFLLVL YLNRKGIKKW MHNIRDACRD
410 420
HMEGYHYRYE INADPRLTNL SSNSDV
Length:426
Mass (Da):46,451
Last modified:July 27, 2011 - v3
Checksum:iA03A76377F68D2A4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471A → D in CAA09931 (PubMed:10366710).Curated
Sequence conflicti161 – 1611A → V in CAA09931 (PubMed:10366710).Curated
Sequence conflicti220 – 2201R → C in AAH58198 (PubMed:15489334).Curated
Sequence conflicti288 – 2881H → Q in CAA09931 (PubMed:10366710).Curated
Sequence conflicti288 – 2881H → Q in AAH58198 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012160 Genomic DNA. Translation: CAA09931.1.
AK051162 mRNA. Translation: BAC34540.1.
AK143519 mRNA. Translation: BAE25413.1.
BC058198 mRNA. Translation: AAH58198.1.
CCDSiCCDS23380.1.
RefSeqiNP_001158264.1. NM_001164792.1.
NP_035757.2. NM_011627.4.
UniGeneiMm.20864.
Mm.484180.

Genome annotation databases

EnsembliENSMUST00000006559; ENSMUSP00000006559; ENSMUSG00000035274.
ENSMUST00000098500; ENSMUSP00000096101; ENSMUSG00000035274.
GeneIDi21983.
KEGGimmu:21983.
UCSCiuc009qwz.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ012160 Genomic DNA. Translation: CAA09931.1.
AK051162 mRNA. Translation: BAC34540.1.
AK143519 mRNA. Translation: BAE25413.1.
BC058198 mRNA. Translation: AAH58198.1.
CCDSiCCDS23380.1.
RefSeqiNP_001158264.1. NM_001164792.1.
NP_035757.2. NM_011627.4.
UniGeneiMm.20864.
Mm.484180.

3D structure databases

ProteinModelPortaliQ9Z0L0.
SMRiQ9Z0L0. Positions 59-350.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9Z0L0.

Proteomic databases

MaxQBiQ9Z0L0.
PaxDbiQ9Z0L0.
PRIDEiQ9Z0L0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006559; ENSMUSP00000006559; ENSMUSG00000035274.
ENSMUST00000098500; ENSMUSP00000096101; ENSMUSG00000035274.
GeneIDi21983.
KEGGimmu:21983.
UCSCiuc009qwz.2. mouse.

Organism-specific databases

CTDi7162.
MGIiMGI:1341264. Tpbg.

Phylogenomic databases

eggNOGiNOG301461.
GeneTreeiENSGT00620000088023.
HOGENOMiHOG000013090.
HOVERGENiHBG053843.
InParanoidiQ9Z0L0.
OMAiTYVSFRN.
OrthoDBiEOG7QNVM8.
TreeFamiTF351115.

Miscellaneous databases

NextBioi301700.
PROiQ9Z0L0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z0L0.
CleanExiMM_TPBG.
ExpressionAtlasiQ9Z0L0. baseline and differential.
GenevestigatoriQ9Z0L0.

Family and domain databases

InterProiIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRR-contain_N.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF01462. LRRNT. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
SM00082. LRRCT. 1 hit.
SM00013. LRRNT. 1 hit.
[Graphical view]
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Organisation of the mouse and human 5T4 oncofetal leucine-rich glycoprotein gene and expression in foetal and adult murine tissues."
    King K.W., Sheppard F.C., Westwater C., Stern P.L., Myers K.A.
    Biochim. Biophys. Acta 1445:257-270(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: 129/Sv.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Spinal ganglion.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  4. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-281.

Entry informationi

Entry nameiTPBG_MOUSE
AccessioniPrimary (citable) accession number: Q9Z0L0
Secondary accession number(s): Q3UPI2, Q6PE98, Q8BQA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: July 27, 2011
Last modified: April 29, 2015
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.